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作物杂志,2026, 第4期: 46–56 doi: 10.16035/j.issn.1001-7283.2026.04.006

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

向日葵Trihelix转录因子家族鉴定及表达分析

张曼1(), 董海潇2, 田娟1, 冯博1, 于学鹏1, 王祉诺1, 张雷1, 巴桑平措1, 李雪英1(), 李慧英1()   

  1. 1 吉林省白城市农业科学院， 137000， 吉林白城
    2 吉林大学植物科学学院， 130062， 吉林长春
  • 收稿日期:2025-05-07 修回日期:2025-08-19 出版日期:2026-08-15 发布日期:2026-08-11
  • 通讯作者: 李雪英,李慧英
  • 作者简介:张曼，主要从事作物遗传育种研究，E-mail：18844696509@163.com
  • 基金资助:
    国家特色油料产业技术体系(CARS-14)

Identification and Expression Analysis of Trihelix Transcription Factor Family in Sunflower

Zhang Man1(), Dong Haixiao2, Tian Juan1, Feng Bo1, Yu Xuepeng1, Wang Zhinuo1, Zhang Lei1, Basangpingcuo 1, Li Xueying1(), Li Huiying1()   

  1. 1 Baicheng Academy of Agricultural Sciences, Baicheng 137000, Jilin, China
    2 College of Plant Science, Jilin University, Changchun 130062, Jilin, China
  • Received:2025-05-07 Revised:2025-08-19 Online:2026-08-15 Published:2026-08-11
  • Contact: Li Xueying,Li Huiying

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摘要：

Trihelix基因家族在植物生长发育和逆境胁迫响应中具有重要作用，但目前缺乏针对向日葵（Helianthus annuus L.）trihelix家族在全基因组水平的系统鉴定与表达特征研究。通过生物信息学方法，对向日葵trihelix基因家族进行全基因组鉴定，并对其染色体定位、基因复制、系统进化、基因结构、保守基序和胁迫条件下的表达模式进行分析。结果表明，在向日葵基因组中共鉴定到46个trihelix家族成员，它们不均匀分布在15条染色体上。其中有3对串联复制基因和6对片段复制基因。系统进化分析分为5个亚家族，且同一亚家族的家族成员具有相似的基因结构和保守基序。36个向日葵trihelix基因在根、茎、叶、花和芽中具有表达活性。基于转录组测序（RNA-seq）数据分析，29个trihelix基因显著响应向日葵列当寄生胁迫。

关键词: 向日葵, Trihelix基因家族, 生物信息学, 列当, 表达模式

Abstract:

Trihelix transcription factors play important roles in plant growth, development, and various stress responses. However, few studies have been conducted on the systematic identification and expression characteristics of trihelix gene family in sunflower (Helianthus annuus L.). In this study, based on bioinformatics methods, the trihelix genes in sunflower genome were identified and characterized, and chromosomal localization, duplication events, phylogenetic analysis, gene structures, conserved motifs and expression patterns under stress conditions were analyzed. The results showed that a total of 46 trihelix genes were identified in the sunflower genome, unevenly distributed across 15 chromosomes. Among them, three pairs of tandemly duplicated genes and six pairs of segmentally duplicated genes were detected. According to phylogenetic analysis, sunflower trihelix family was divided into five subfamilies, and the members of the same subfamily have similar gene structures and conserved motifs. Thirty-six sunflower trihelix genes were expressed in all five organs (root, stem, leaf, flower and bud). Based on transcriptome sequencing (RNA-seq) data, 29 trihelix genes significantly responded to Orobanche cumana Wallr. stress.

Key words: Sunflower, Trihelix gene family, Bioinformatics, Orobanche cumana Wallr., Expression pattern

表1

qRT-PCR所需引物

基因名称Gene name 正向引物（5’-3’）Forward primer (5’-3’) 反向引物（5’-3’）Reverse primer (5’-3’)
HaTrihelix6 GCCGTGGCAGAGGATGAA GCCGTATCGGAAACTTGAT
HaTrihelix12 ACGTCGTCTCAGCTCCATC TCACCGTCATCAGTAAATCCA
HaTrihelix15 TTGAAGCGATTGTGAAAGAG TGCCGAGACAGACGATAA
HaTrihelix16 ATGACTGAACCTCCACCG CAAATCCCTCGTCTCCTG
HaTrihelix19 ACAATGCGGGAAGTAGAG GCGAAGAGCTTAGCGATC
HaTrihelix20 CCACCACAACCGACACCA CGGATACCGATACAGATACAGGA
HaTrihelix26 ACCCGAACCCTGATGTGA CCCTCGCCAACTCCTTAT
HaTrihelix27 TGAATGAAACCGCTCTGC ACCGTTGTCATCATCCCT
HaTrihelix28 GCCCGCAATGCAGTTATT GTCGGTTGTGCTGGTGGT
HaTrihelix31 CCACCAAGTCAGCCTCATG CAGCACTCAGACTCGCAGTT
18S rRNA CTACCACATCCAAGGAAGGCAG CGACAGAAGGGACGAGTAAACC

表2

向日葵trihelix家族蛋白的理化性质

基因名称
Gene name		 基因登录号
Gene ID		 氨基酸数目
Amino acid
number		 分子量
Molecular
weight (kDa)		 等电点
Isoelectric
point		 不稳定系数
Instability
index		 亲水系数
Hydrophilicity
coefficient		 亚细胞定位预测
Subcellular location
prediction
HaTrihelix1 HanXRQr2_Chr01g0009681 251 30.12 7.44 57.26 -1.127 细胞核
HaTrihelix2 HanXRQr2_Chr01g0010711 306 33.75 8.19 46.31 -0.802 细胞核
HaTrihelix3 HanXRQr2_Chr01g0028301 290 33.40 8.60 54.58 -0.883 叶绿体、细胞核
HaTrihelix4 HanXRQr2_Chr01g0028311 347 38.41 8.88 48.68 -0.801 细胞核
HaTrihelix5 HanXRQr2_Chr01g0031811 371 42.72 5.57 47.30 -1.065 细胞核
HaTrihelix6 HanXRQr2_Chr01g0041281 546 62.21 8.28 49.09 -0.622 叶绿体、细胞核
HaTrihelix7 HanXRQr2_Chr01g0041321 188 20.81 9.24 34.10 -0.713 细胞核
HaTrihelix8 HanXRQr2_Chr01g0041331 517 56.29 7.52 32.24 -0.449 叶绿体
HaTrihelix9 HanXRQr2_Chr01g0041541 426 49.22 7.46 60.04 -0.763 叶绿体、细胞核
HaTrihelix10 HanXRQr2_Chr02g0047701 390 44.23 4.63 42.36 -1.203 细胞核
HaTrihelix11 HanXRQr2_Chr02g0064991 53 6.11 7.26 36.06 -0.743 细胞核
HaTrihelix12 HanXRQr2_Chr02g0065041 593 65.41 6.04 61.03 -0.859 细胞核
HaTrihelix13 HanXRQr2_Chr03g0088951 379 44.61 4.55 41.14 -1.195 细胞核
HaTrihelix14 HanXRQr2_Chr03g0095111 916 102.44 8.26 47.23 -0.391 叶绿体
HaTrihelix15 HanXRQr2_Chr03g0123561 248 27.74 4.54 57.25 -0.617 细胞核
HaTrihelix16 HanXRQr2_Chr03g0124371 315 36.67 8.66 61.91 -1.210 细胞核
HaTrihelix17 HanXRQr2_Chr03g0132841 380 42.89 10.08 42.61 -0.979 细胞核
HaTrihelix18 HanXRQr2_Chr04g0169001 596 66.77 6.10 87.15 -1.220 细胞核
HaTrihelix19 HanXRQr2_Chr04g0172241 369 41.03 9.89 59.84 -0.763 细胞核
HaTrihelix20 HanXRQr2_Chr05g0216931 494 52.72 8.45 37.93 -0.142 叶绿体
HaTrihelix21 HanXRQr2_Chr05g0227381 339 38.80 4.99 50.22 -1.011 细胞核
HaTrihelix22 HanXRQr2_Chr05g0236141 650 72.51 7.50 72.61 -0.979 细胞核
HaTrihelix23 HanXRQr2_Chr06g0242381 375 42.34 6.80 42.90 -0.893 叶绿体、细胞核
HaTrihelix24 HanXRQr2_Chr06g0253741 373 42.14 6.28 37.49 -0.643 叶绿体、细胞核
HaTrihelix25 HanXRQr2_Chr07g0299811 412 46.36 10.00 73.35 -1.217 细胞核
HaTrihelix26 HanXRQr2_Chr09g0382641 249 28.48 10.28 26.94 -1.052 叶绿体、细胞核
HaTrihelix27 HanXRQr2_Chr09g0415361 352 40.65 4.55 42.12 -1.334 细胞核
HaTrihelix28 HanXRQr2_Chr10g0420111 632 70.22 6.29 75.62 -0.956 细胞核
HaTrihelix29 HanXRQr2_Chr10g0430831 239 28.97 8.65 71.37 -1.215 细胞核
HaTrihelix30 HanXRQr2_Chr11g0466401 290 33.40 8.60 54.58 -0.883 叶绿体、细胞核
HaTrihelix31 HanXRQr2_Chr11g0505381 309 34.94 9.43 41.31 -0.877 叶绿体、细胞核
HaTrihelix32 HanXRQr2_Chr11g0515851 411 46.36 6.02 53.48 -1.042 细胞核
HaTrihelix33 HanXRQr2_Chr11g0516471 424 48.17 7.83 45.52 -0.859 细胞质、过氧化物酶体
HaTrihelix34 HanXRQr2_Chr12g0555331 296 33.83 9.04 65.97 -1.024 细胞核
HaTrihelix35 HanXRQr2_Chr12g0564671 304 34.18 5.03 61.58 -0.782 细胞核
HaTrihelix36 HanXRQr2_Chr13g0619331 510 59.83 6.59 59.59 -1.035 细胞核
HaTrihelix37 HanXRQr2_Chr15g0709391 393 44.78 8.24 47.19 -0.932 细胞核
HaTrihelix38 HanXRQr2_Chr15g0715481 430 49.47 6.33 55.32 -0.881 细胞核
HaTrihelix39 HanXRQr2_Chr16g0726631 362 41.50 4.47 33.58 -1.129 细胞核
HaTrihelix40 HanXRQr2_Chr16g0741871 273 32.45 5.78 56.67 -1.149 细胞核
HaTrihelix41 HanXRQr2_Chr16g0753561 422 48.34 6.33 63.42 -0.872 细胞核
HaTrihelix42 HanXRQr2_Chr16g0774461 273 30.76 5.13 49.31 -0.806 细胞核
HaTrihelix43 HanXRQr2_Chr17g0779611 345 39.11 9.44 31.35 -0.766 叶绿体
HaTrihelix44 HanXRQr2_Chr17g0786401 254 28.67 5.60 44.26 -0.729 细胞核
HaTrihelix45 HanXRQr2_Chr17g0786421 252 28.38 5.59 43.24 -0.728 细胞核
HaTrihelix46 HanXRQr2_Chr17g0814401 368 40.99 10.06 60.70 -0.867 细胞核

图1

向日葵trihelix基因家族的共线性分析 红色字体的基因为串联复制基因对，长红线表示片段复制基因对。

图2

3个物种间trihelix家族基因共线性分析

图3

向日葵、拟南芥和水稻trihelix基因家族的系统进化树分析

图4

向日葵trihelix家族成员基因结构和保守基序分析

图5

向日葵trihelix转录因子三螺旋结构域氨基酸序列比对

图6

向日葵trihelix家族基因组织表达模式分析 不同小写字母表示在P < 0.05水平差异显著。

图7

向日葵trihelix家族基因在列当胁迫下的表达分析 hpi为接种后小时数；“*”：P < 0.05，“**”：P < 0.01。

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