EMS诱变刺荞的形态突变体鉴定与分析

doi:10.16035/j.issn.1001-7283.2019.03.007

作物杂志,2019, 第3期: 37–41 doi: 10.16035/j.issn.1001-7283.2019.03.007

• 遗传育种·种质资源·生物技术 • 上一篇下一篇

EMS诱变刺荞的形态突变体鉴定与分析

马名川,刘龙龙,张丽君,崔林,周建萍

山西省农业科学院农作物品种资源研究所/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,030031,山西太原

收稿日期:2019-01-02 修回日期:2019-04-04 出版日期:2019-06-15 发布日期:2019-06-12
通讯作者: 周建萍
作者简介:马名川,助理研究员,主要从事荞麦燕麦种质资源创新与利用研究;
基金资助:
国家燕麦荞麦产业技术体系(CARS-07-A2);山西省农业科学院特色农业技术攻关项目(YGG17063);山西省重点研发计划重点项目子项目(201603D21102);山西省农作物种质资源共享服务平台运行补助(201705D121019-5);山西省农作物种质资源补充征集(YGG17061);山西省农作物种质资源共享服务平台(201605D121018)

Morphological Identification and Analysis of EMS-Induced Mutants from Ciqiao

Ma Mingchuan,Liu Longlong,Zhang Lijun,Cui Lin,Zhou Jianping

Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, Shanxi, China

Received:2019-01-02 Revised:2019-04-04 Online:2019-06-15 Published:2019-06-12
Contact: Jianping Zhou

摘要/Abstract

摘要：

为构建苦荞EMS突变体库和为苦荞功能基因组学研究准备基础材料,利用化学诱变剂甲基磺酸乙酯（ethyl methane sulfonate,EMS）诱变处理刺荞种子,对获得的M₂代材料进行生物学性状与农艺性状鉴定,并对部分M₂代材料播种家系进行验证。对M₂代全生育期田间表型进行观察鉴定,共获得480份突变体材料,包括子粒、叶片、茎秆及生育期等生物学特性与主要农艺性状突变体,变异类型丰富,尤其是首次发现了子粒多棱苦荞新材料,并发现了自然突变中少见的变异类型,如子粒开裂等变异类型。所构建的刺荞EMS突变群体较理想,可望有效用于苦荞功能基因组研究和苦荞遗传改良。

关键词: 苦荞, EMS, 突变体, 鉴定

Abstract:

The mutant library provide the basic material of functional genomics. In this study, seeds of tartary buckwheat Ciqiao was treated by chemical mutagen Ethyl methane sulfonate (EMS). Mutation plants were screened in M₂generation in terms of morphology. Some of mutants identified from M₂ plants were validated through M₃ generation. The 480 mutant individuals were obtained including grain, leaf, and stem mutants, mature period were obtained with the comparison to the wild type. Some of them were related to important agronomic traits and biological characters, specifically, the mutant of polygonal grain was the first discovered and some mutants were rarely observed in natural variation, such as grain cracking. The mutant population of Ciqiao induced with EMS were ideal mutant population and they would be efficiently used in functional genome research and genetic improvement of tartary buckwheat.

Key words: Tartary buckwheat, Ethyl methane sulfonate (EMS), Mutant, Identification

马名川,刘龙龙,张丽君,崔林,周建萍. EMS诱变刺荞的形态突变体鉴定与分析[J]. 作物杂志, 2019 (3): 37–41

Ma Mingchuan,Liu Longlong,Zhang Lijun,Cui Lin,Zhou Jianping. Morphological Identification and Analysis of EMS-Induced Mutants from Ciqiao[J]. Crops, 2019(3): 37–41

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EMS诱变刺荞的形态突变体鉴定与分析

Morphological Identification and Analysis of EMS-Induced Mutants from Ciqiao

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