细菌HT-6的鉴定及其对马铃薯致病疫霉抑制稳定性的研究

doi:10.16035/j.issn.1001-7283.2019.06.026

摘要/Abstract

摘要：

为明确马铃薯致病疫霉拮抗菌HT-6的生物学特性及抑菌潜力,通过对其在不同培养基上的菌落特征观察、生理生化特性检测及16S rDNA序列分析,确定该菌株的分类地位,并测试了不同温度、pH、紫外线以及传代对该菌株菌液抑制致病疫霉菌丝生长的影响。综合菌落特征、生理生化特性及16S rDNA序列比对结果,确定细菌HT-6为枯草芽孢杆菌（Bacillus subtilis）;该菌株菌液中抑制致病疫霉菌丝生长的物质经100℃处理60min、pH值6~8处理24h、30W紫外线照射8h、连续传代10次,抑菌率仍保持在80%以上。这些结果表明,枯草芽孢杆菌HT-6及其产生的抑菌物质除不耐酸碱外,耐热、抗紫外线且适于长期保存,将其开发成预防马铃薯晚疫病的生防制剂具有巨大潜力。

关键词: 马铃薯, 菌种鉴定, 16S rDNA, 抑菌稳定性, 枯草芽孢杆菌

Abstract:

In order to clarify the biological characteristics and antagonistic stability of bacterium HT-6 strain on Phytophthora infestans, this experiment was conducted to observe its colony features on different media, detect physiological and biochemical characteristics and analyze 16S rDNA sequence, to determine its classification status. The effects of different temperature, pH, ultraviolet light, and continuous passage on the mycelial growth of Phytophthora infestans were tested. The HT-6 strain was identified as Bacillus subtilis based on results of its colony properties, physiological and biochemical characteristics and 16S rDNA sequence. The inhibition rates of HT-6 strain and its metabolites on P. infestans could keep more than 80% after treatment with 100℃ for 60min, pH 6-8 for 24h, 30W ultraviolet irradiation for 8h, and continuous 10 passages. These results showed that HT-6 strain and its metabolites had thermostability, ultraviolet resistance, and could store for a long time, except toleration acid and alkali. It has great potential value to develop it into a biological control agent for the prevention of potato late blight.

Key words: Potato, Strain identification, 16S rDNA, Bacteriostatic stability, Bacillus subtilis

张聪颖,蒋继志,梁娇,乔柳,黄杰. 细菌HT-6的鉴定及其对马铃薯致病疫霉抑制稳定性的研究[J]. 作物杂志, 2019 (6): 162–167

Zhang Congying,Jiang Jizhi,Liang Jiao,Qiao Liu,Huang Jie. Identification of Bacterium HT-6 and Its Antagonistic Stability against Phytophthora infestans[J]. Crops, 2019(6): 162–167

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试验Test	HT-6	BS224^[16]	LXY-6^[17]	JY-1^[18]
淀粉水解试验 Starch hydrolysis test	+	+	+	+
L-阿拉伯糖利用试验 L-arabionse utilization test	+	+	+	+
葡萄糖利用试验 Glucose utilization test	+	+	+	+
乳糖利用试验 Lactose utilization test	+	+	ND	-
木糖利用试验 Xylose utilization test	+	+	+	+
V. P试验V. P test	+	+	-	+
接触酶试验 Contact enzyme test	+	+	+	+
H₂S试验H₂S test	+	+	+	ND
溶血试验Hemolysis test	+	+	+	+
5% NaCl耐受试验 5% NaCl tolerance test	+	+	+	+
14% NaCl耐受试验 14% NaCl tolerance test	-	-	+	-
30% NaCl耐受试验 30% NaCl tolerance test	-	-	+	-
蛋白水解试验 Proteolysis test	+	+	ND	ND
色素试验Pigment test	-	-	ND	ND