Crops ›› 2017, Vol. 33 ›› Issue (3): 69-74.doi: 10.16035/j.issn.1001-7283.2017.03.013

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Water Deficit Stress and Osmotic Substances Accumulation of Vicia sativa L. under Cadmium Stress

Rui Haiyun1,2,Zhang Xingxing2,Shen Zhenguo2,Zhang Fenqin3   

  1. 1 College of Pharmacy and Chemistry & Chemical Engineering,Taizhou University,Taizhou 225300,Jiangsu,China;
    2 College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
    3 College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,Gansu,China
  • Received:2017-04-11 Revised:2017-04-27 Online:2017-06-15 Published:2018-08-26

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Abstract:

We investigated the effects of cadmium stress on water content and osmotic substances accumulation in two Vicia sativa varieties (cadmium-tolerant variety L3, cadmium-sensitive variety ZM), using solution culture experiments, and studied the tolerance of two varieties to water deficit stress. The results showed that water content decreased 1.27% and 4.66% in the roots, and 4.06% and 5.46% in the overground part of L3 and ZM, respectively. Content of total amino acid, proline and reducing sugar increased in the roots by two varieties and content of total amino acid increased in the stems by two varieties under cadmium stress, and the changes were more significant in ZM than in L3; Content of proline and reducing sugar increased significantly in the leaf of ZM but not in the leaf of L3. Meanwhile, Cd-sensitive variety ZM was more tolerant to water deficit stress than Cd-tolerant variety L3. The results indicated that accumulation of osmotic substances in V. sativa was a positive response to water deficit caused by cadmium stress. The tolerance to water deficit stress was not the main factor influencing the cadmium tolerance of V. sativa.

Key words: Cadmium, Vicia sativa L., Water deficit stess, Osmotic adjustment

Fig.1

Changes of water content of roots and overground part in two V. sativa varieties under cadmium stress"

Fig.2

Changes of total amino acids content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Fig.3

Changes of proline content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Fig.4

Changes of reducing sugar content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Table 1

The changes of biomass of two V. sativa varieties under water deficit stress"

品种Variety 处理
Treatment		 根长(cm)
Root length		 株高(cm)
Plant height		 根的鲜重(mg/plant)
Root fresh weight		 地上部鲜重(mg/plant)
Overground part fresh weight		 根的干重(mg/plant)
Root dry weight		 地上部干重(mg/plant)
Overground part dry weight
L3 对照Control 30.25±1.10a 38.91±0.74b 314.73±32.28a 463.37±18.57c 16.92±2.20a 51.81±0.45a
10%PEG-6000 22.74±0.45b 35.51±1.47c 253.16±8.86b 402.83±4.62d 17.04±0.31a 46.55±1.02b
ZM 对照Control 31.22±0.13a 44.08±2.02a 299.73±6.01a 565.33±11.85a 15.25±0.52a 54.77±1.06a
10%PEG-6000 20.55±1.74c 41.89±1.85ab 260.06±15.13b 527.03±22.02b 16.29±0.12a 54.48±1.99a
[1] Thapa G, Sadhukhan A, Panda S K , et al. Molecular mechanistic model of plant heavy metal tolerance. Biometals, 2012,25, 489-505.
doi: 10.1007/s10534-012-9541-y pmid: 22481367
[2] Mohamed A A, Castagna A, Ranieri L S , et al. Cadmium tolerance in Brassica juncea roots and shoots is affected by antioxidant status and phytochelatin biosynthesis. Plant Physiology and Biochemistry, 2012,57:15-22.
doi: 10.1016/j.plaphy.2012.05.002
[3] Barcelò J, Poschenrieder C . Plant-water relations as affected by heavy metal stress:a review. Journal of Plant Nutrition, 1990,13:1-37.
doi: 10.1080/01904169009364057
[4] Costa G, Morel J L . Water relations,gas exchange and amino acid content in Cd-treated lettuce. Plant Physiology and Biochemistry, 1994,32:561-570.
doi: 10.1104/pp.105.3.1027
[5] Perfus-Barbeoch L, Leonhard N, Vavasseur A , et al. Heavy metal toxicity:cadmium permeates through calcium channels and disturbs the plant water status. The Plant Journal, 2002,32(4):539-548.
doi: 10.1046/j.1365-313X.2002.01442.x
[6] 刘艳, 陈贵林, 蔡贵芳 , 等. 干旱胁迫对甘草幼苗生长和渗透调节物质含量的影响. 西北植物学报, 2011,31(11):2259-2264.
[7] Silva E N , Ferreira-Silva S L,Viégas R A,et al.The role of organic and inorganic solutes in the osmotic adjustment of drought-stressed Jatropha curcas plants. Environmental and Experimental Botany, 2010,69:279-285.
doi: 10.1016/j.envexpbot.2010.05.001
[8] 张芬琴 . 镉胁迫对二种不同耐性豆科植物生长与活性氧代谢的影响及水杨酸对镉毒害的缓解效应. 南京:南京农业大学, 2009.
[9] 李合生, 孙群, 赵世杰 , 等. 植物生理生化实验原理和技术.北京: 高等教育出版社, 2000.
[10] Sanita di Toppi L, Gabbrielli R . Response to cadmium in higher plants. Environmental and Experimental Botany, 1999,41:105-130.
doi: 10.1016/S0098-8472(98)00058-6
[11] Zoghlami L B, Djebali W, Abbes Z , et al. Metabolite modifications in Solanum lycopersicum roots and leaves under cadmium stress. African Journal of Biotechnology, 2011,10(4):567-579.
[12] Hsu Y T, Kao C H . Changes in protein and amino acid contents in two cultivars of rice seedlings with different apparent tolerance to cadmium. Plant Growth Regulation, 2003,40:147-155.
doi: 10.1023/A:1024248021314
[13] Shah K, Dubey R S . Cadmium alters phosphate level and suppresses activity of phosphorolytic enzymes in germinating rice seeds. Journal of Agronomy and Crop Science, 1997,179:35-45.
doi: 10.1111/j.1439-037X.1997.tb01145.x
[14] Xu J, Yin H X, Li X . Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator Solanum nigrum L. Plant Cell Reports, 2009,28:325-333.
doi: 10.1007/s00299-008-0643-5
[15] Polge C, Jaquinod M, Holzer F , et al. Evidence for the existence in Arabidopsis thaliana of the proteasome proteolytic pathway-activation in response to cadmium. Journal of Biological Chemistry, 2009,284:35412-35424.
doi: 10.1074/jbc.M109.035394
[16] Sarry J E, Kuhn L, Ducruix C , et al. The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses. Proteomics, 2006,6(7):2180-2198.
doi: 10.1002/(ISSN)1615-9861
[17] 芮海云, 庄凯, 沈振国 , 等. 两个箭舌豌豆品种根响应镉胁迫的蛋白质组学分析. 植物生理学报, 2016,52(7):1089-1098.
[18] Talanova V V, Titov A F, Boeva N P . Effect of increasing concentrations of lead and cadmium on cucumber seedlings. Biologia Plantarum, 2000,43:441-444.
doi: 10.1023/A:1026735603890
[19] Zengin F K, Munzuroglu O . Effects of some heavy metals on content of chlorophyll,proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biologica Cracoviensia Series Botanica, 2005,47:157-164.
[20] Chen G X, Wang N, Shao Z G , et al. Effects of phosphorus nutrition on physiological activity of Nymphaea tetragona Georgi and Trapa bispinosa Roxb.leaves. Journal of Nanjing Normal University (Natural Science) , 2002,25(1):71-77.
[21] Schat H, Sharma S S, Vooijs R . Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris. Physiologia Plantarum, 1997,101:477-482.
doi: 10.1111/ppl.1997.101.issue-3
[22] Kastori R, Petrovic M, Petrovic N . Effect of excess lead,cadmium,copper and zinc on water relations in sunflower. Journal of Plant Nutrition, 1992,15:2427-2439.
doi: 10.1080/01904169209364485
[23] Zhang F, Li X, Wang C , et al. Effect of cadmium on autoxidation rate of tissue and inducing accumulation of free proline in seedlings of mung bean. Journal of Plant Nutrition, 2000,23:356-368.
[24] Sharma S S, Dietz K J . The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany, 2007,57(4):711-726.
[25] Kaur G, Asthir B . Proline:a key player in plant abiotic stress tolerance. Biologia Plantarum, 2015,59(4):609-619.
doi: 10.1007/s10535-015-0549-3
[26] Couée I, Sulmon C, Gouesbet G , et al. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany, 2006,57(3):449-459.
doi: 10.1093/jxb/erj027
[27] Silva E N, Ribeiro R V , Ferreira-Silva S L,et al.Coordinate changes in photosynthesis,sugar accumulation and antioxidative enzymes improve the performance of Jatropha curcas plants under drought stress. Biomass and Bioenergy, 2012,5:270-279.
[28] Kieffer P, Dommes J, Hoffmann L , et al. Quantitative changes in protein expression of cadmium-exposed poplar plants. Proteomics, 2009,8(12):2514-2530.
doi: 10.1002/pmic.200701110 pmid: 18563750
[29] McCormick A J, Watt D A, Cramer M D . Supply and demand:sink regulation of sugar accumulation in sugarcane. Journal of Experimental Botany, 2009,60(2):357-364.
doi: 10.1093/jxb/ern310 pmid: 19050062
[30] Oono Y, Yazawa T, Kawahara Y , et al. Genome-wide transcriptome analysis reveals that cadmium stress signaling controls the expression of genes in drought stress signal pathways in rice. Plos One, 2014,9(5):e96946.
doi: 10.1371/journal.pone.0096946
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