Crops ›› 2017, Vol. 33 ›› Issue (3): 69-74.doi: 10.16035/j.issn.1001-7283.2017.03.013

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Water Deficit Stress and Osmotic Substances Accumulation of Vicia sativa L. under Cadmium Stress

Rui Haiyun1,2,Zhang Xingxing2,Shen Zhenguo2,Zhang Fenqin3   

  1. 1 College of Pharmacy and Chemistry & Chemical Engineering,Taizhou University,Taizhou 225300,Jiangsu,China;
    2 College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
    3 College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,Gansu,China
  • Received:2017-04-11 Revised:2017-04-27 Online:2017-06-15 Published:2018-08-26

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Abstract:

We investigated the effects of cadmium stress on water content and osmotic substances accumulation in two Vicia sativa varieties (cadmium-tolerant variety L3, cadmium-sensitive variety ZM), using solution culture experiments, and studied the tolerance of two varieties to water deficit stress. The results showed that water content decreased 1.27% and 4.66% in the roots, and 4.06% and 5.46% in the overground part of L3 and ZM, respectively. Content of total amino acid, proline and reducing sugar increased in the roots by two varieties and content of total amino acid increased in the stems by two varieties under cadmium stress, and the changes were more significant in ZM than in L3; Content of proline and reducing sugar increased significantly in the leaf of ZM but not in the leaf of L3. Meanwhile, Cd-sensitive variety ZM was more tolerant to water deficit stress than Cd-tolerant variety L3. The results indicated that accumulation of osmotic substances in V. sativa was a positive response to water deficit caused by cadmium stress. The tolerance to water deficit stress was not the main factor influencing the cadmium tolerance of V. sativa.

Key words: Cadmium, Vicia sativa L., Water deficit stess, Osmotic adjustment

Fig.1

Changes of water content of roots and overground part in two V. sativa varieties under cadmium stress"

Fig.2

Changes of total amino acids content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Fig.3

Changes of proline content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Fig.4

Changes of reducing sugar content of roots, stems and leaves in two V. sativa varieties under cadmium stress"

Table 1

The changes of biomass of two V. sativa varieties under water deficit stress"

品种Variety 处理
Treatment		 根长(cm)
Root length		 株高(cm)
Plant height		 根的鲜重(mg/plant)
Root fresh weight		 地上部鲜重(mg/plant)
Overground part fresh weight		 根的干重(mg/plant)
Root dry weight		 地上部干重(mg/plant)
Overground part dry weight
L3 对照Control 30.25±1.10a 38.91±0.74b 314.73±32.28a 463.37±18.57c 16.92±2.20a 51.81±0.45a
10%PEG-6000 22.74±0.45b 35.51±1.47c 253.16±8.86b 402.83±4.62d 17.04±0.31a 46.55±1.02b
ZM 对照Control 31.22±0.13a 44.08±2.02a 299.73±6.01a 565.33±11.85a 15.25±0.52a 54.77±1.06a
10%PEG-6000 20.55±1.74c 41.89±1.85ab 260.06±15.13b 527.03±22.02b 16.29±0.12a 54.48±1.99a
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