根系分泌物对根际土壤关键氮转化过程的影响

doi:10.16035/j.issn.1001-7283.2021.06.001

Abstract

Abstract:

Root exudates play an important role in soil nitrogen cycle, N₂O emissions, and plants nitrogen use efficiency, and it is also at the forefront of soil science, plant nutrition, environmental science and interdisciplinary. In order to understand the effects of root exudates on the nitrogen cycle in the soil, this position paper presents the fractions and the methods to study root exudates. The effects of root exudates on the most important nitrogen conversion processes in soils were investigated. The ability to suppress soil nitrification, denitrification and N₂O emissions through the release of exudates from plant roots is highlighted and the further prospects are suggested. This work will provide some references for the investigation of the nitrogen cycle mechanism of interaction effects between soil-plant-microorganisms to further improve the utilization rate of nitrogen fertilizer and reduce the environmental pollution caused by nitrogen fertilizer.

Key words: Root exudates, Nitrogen, Mineralization and immobilization, Nitrification, Denitrification, N₂O emission

Wang Rui, Chen Shiyong, Chen Zhiqing, Cui Peiyuan, Lu Hao, Yang Yanju, Zhang Haipeng, Zhang Hongcheng. Effects of Root Exudates on Key Processes of Soil Nitrogen Cycling: A Review[J].Crops, 2021, 37(6): 1-8.

Figures/Tables 2

Fig.1

Table 1

References 85

[1]	Sutton M A, Oenema O, Erisman J W, et al. Too much of a good thing. Nature, 2011, 472(2):159-161. doi: 10.1038/472159a
[2]	Hungate B A, Dukes J S, Shaw M R, et al. Nitrogen and climate change. Science, 2003, 302(5650):1512-1513. pmid: 14645831
[3]	Cai Z C, Shan Y H, Xu H. Effects of nitrogen fertilization on CH₄ emissions from rice fields. Soil Science and Plant Nutrition, 2007, 53(4):353-361. doi: 10.1111/j.1747-0765.2007.00153.x
[4]	Date O P. Guatemala：FAO/WFP crop and food security assessment mission to Guatemala. (2010-02-23) [2021-04-01]. http://www.fao.org/giews/.
[5]	Peng S B, Buresh R J, Huang J L, et al. Strategies for overcoming low agronomic nitrogen use efficiency in irrigated rice systems in China. Field Crops Research, 2006, 96(1):37-47. doi: 10.1016/j.fcr.2005.05.004
[6]	Chen D, Suter H C, Islam A, et al. Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture:a review of enhanced efficiency fertilisers. Australian Journal of Soil Research, 2008, 46(4):289-301.
[7]	Geng J B, Chen J Q, Sun Y B, et al. Controlled release urea improved nitrogen use efficiency and yield of wheat and corn. Soil Fertility and Crop Nutrition, 2016, 108(4):1666-1673.
[8]	Zhu T B, Zhang J B, Cai Z C. The contribution of nitrogen transformation processes to total N₂O emissions from soils used for intensive vegetable cultivation. Plant and Soil, 2011, 343(1):313-327. doi: 10.1007/s11104-011-0720-3
[9]	Subbarao G V, Sahrawat K L, Nakahara K, et al. A paradigm shift towards low-nitrifying production systems:the role of biological nitrification inhibition. Annals of Botany, 2013, 112(3):297-316. doi: 10.1093/aob/mcs230
[10]	Ju X T, Xing G X, Chen X P, et al. Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proceedings of National Academy of Sciences of the United States of America, 2009, 106(2):3041-3046.
[11]	Tilman D, Fargione J, Wolff B, et al. Forecasting agriculturally driven global environmental change. Science, 2001, 292(5515):281-284. pmid: 11303102
[12]	Glass A D M. Nitrogen use efficiency of crop plants:physiological constraints upon nitrogen absorption. Critical Reviews in Plant Sciences, 2003, 22(5):453-470. doi: 10.1080/07352680390243512
[13]	Schafer A, Victor D G. Global passenger travel:implications for carbon dioxide emissions. Energy, 1999, 24(8):657-679. doi: 10.1016/S0360-5442(99)00019-5
[14]	Raun W R, Johnson G V. Improving nitrogen use efficiency for cereal production. Agronomy Journal, 1999, 91(3):357-363. doi: 10.2134/agronj1999.00021962009100030001x
[15]	Li Y, Ouyang J, Wang Y Y, et al. Disruption of the rice nitrate transporter OsNPF2.2 hinders root-to-shoot nitrate transport and vascular development. Scientific Reports, 2015, 5(1):1-10.
[16]	Schlesinger W H. On the fate of anthropogenic nitrogen. Proceedings of National Academy of Sciences of the United States of America, 2009, 106(1):203-208.
[17]	Hofstra N, Bouwman A F. Denitrification in agricultural soils:summarizing published data and estimating global annual rates. Nutrient Cycling Agroecosystems, 2005, 72(3):267-278. doi: 10.1007/s10705-005-3109-y
[18]	Burney J A, Davis S J, Lobell D B. Greenhouse gas mitigation by agricultural intensification. Proceedings of National Academy of Sciences of the United States of America, 2010, 107(26):12052-12057.
[19]	吴林坤, 林向民, 林文雄. 根系分泌物介导下植株-土壤-微生物互作关系研究进展与展望. 植物生态学报, 2014, 38(3):298-310. doi: 10.3724/SP.J.1258.2014.00027
[20]	Paterson E, Gebbing T, Abel C, et al. Rhizodeposition shapes rhizosphere microbial community structure in organic soil. New Phytologist, 2007, 173(3):600-610. doi: 10.1111/j.1469-8137.2006.01931.x pmid: 17244055
[21]	East R. Microbiome:soil science comes to life. Nature, 2013, 501(26):S18-S19. doi: 10.1038/501S18a
[22]	陆玉芳, 施卫明. 生物硝化抑制剂的研究进展及其农业应用前景. 土壤学报, 2021, 58(3):545-557.
[23]	Wu H W, Haig T, Pratley J, et al. Allelo-chemicals in wheat (Triticum aestivum L.):cultivar difference in the exudation of phenolic acids. Journal of Agricultural and Food Chemistry, 2001, 27(1):125-135.
[24]	Materechera S A, Dexter A R, Alston A M. Formation of aggregates by plant roots in homogenized soils. Plant and Soil, 1992, 142(1):69-79. doi: 10.1007/BF00010176
[25]	Soloducho J, Cabaj J. Phenolic compounds hybrid detectors. Journal of Biomaterials and Nanobiotechology, 2013, 4(3):17-27.
[26]	Dessureault-Rompré J, Nowack B, Schulin D, et al. Modified micro suction cup rhizobox approach for the in-situ detection of organic acids in rhizosphere soil solution. Plant and Soil, 2006, 286(1):99-107. doi: 10.1007/s11104-006-9029-z
[27]	Greogory P J, Hinsinger P. New approaches to studying chemical and physical changes in the rhizosphere:An overview. Plant and Soil, 1999, 211(24):1-9. doi: 10.1023/A:1004547401951
[28]	Landi L, Valori F, Ascher J, et al. Root exudate effects on the bacterial communities,CO₂ evolution,nitrogen transformations and ATP content of rhizosphere and bulk soils. Soil Biology and Biochemistry, 2006, 38(3):509-516. doi: 10.1016/j.soilbio.2005.05.021
[29]	Landi L, Badalucco L, Nannipieri P. Changes in inorganic N and CO₂ evolution in soil induced by L-methionine-suphoximine. Soil Biology and Biochemistry, 1995, 27(10):1345-1351. doi: 10.1016/0038-0717(95)00052-G
[30]	Jilling A, Keiluweit M, Gutknecht J, et al. Priming mechanisms providing plants and microbes access to mineral-associated organic matter. Soil Biology and Biochemistry, 2021, 158:108265. doi: 10.1016/j.soilbio.2021.108265
[31]	Nardi S, Concheri G, Pizzeghello D, et al. Soil organic matter mobilization by root exudates. Chemosphere, 2000, 41(5):653-658. pmid: 10834364
[32]	Neal A L, Ahmad S, Gordon-Weeks R, et al. Benzoxazinoids in root exudates of maize attract Pseudomonas putida to the rhizosphere. PLoS ONE, 2012, 7(4):e35489. doi: 10.1371/journal.pone.0035489
[33]	Rasmann S, Turlings T C J. Root signals that mediate mutualistic interactions in the rhizosphere. Current Opinion in Plant Biology, 2016, 32(8):62-68. doi: 10.1016/j.pbi.2016.06.017
[34]	Nardi N, Reniero F, Concheri G. Soil organic matter mobilization by root exudates of three maize hybrids. Chemosphere, 1997, 35(10):2237-2244. doi: 10.1016/S0045-6535(97)00302-0
[35]	Meier I C, Pritchard S G, Brzostek E R, et al. The rhizosphere and hyphosphere differ in their impacts on carbon and nitrogen cycling in forests exposed to elevated CO₂. New Phytologist, 2015, 205(3):1164-1174. doi: 10.1111/nph.13122 pmid: 25348688
[36]	Pathan S I, Ceccherini M T, Pietramellara G, et al. Enzyme activity and microbial community structure in the rhizosphere of two maize lines differing in N use efficiency. Plant and Soil, 2015, 387(28):413-424. doi: 10.1007/s11104-014-2306-3
[37]	Narula N, Kothe E, Behl R K. Role of root exudates in plant-microbe interactions. Journal of Applied Botany and Food Quality, 2009, 82(6):122-130.
[38]	Morris K A, Stark J M, Bugbee B, et al. The invasive annual cheat grass releases more nitrogen than crested wheatgrass through root exudation and senescence. Oecologia, 2016, 181(4):971-983. doi: 10.1007/s00442-015-3544-7
[39]	Taylor A E, Zeglin L H, Dooley S, et al. Evidence for different contributions of archaea and bacteria to the ammonia-oxidizing potential of diverse Oregon soils. Applied Environmental Microbiology, 2010, 76(23):7691-7698. doi: 10.1128/AEM.01324-10
[40]	Dinnes D L, Karlen D L, Jaynes D B, et al. Nitrogen management strategies to reduce nitrate leaching in tile drained Mid-Western soils. Agronomy Journal, 2002, 94(8):153-171. doi: 10.2134/agronj2002.1530
[41]	Hodge A, Robinson D, Fitter A H. Are microorganisms more effective than plants at competing for nitrogen? Trends in Plant Science, 2000, 5(7):304-308. pmid: 10871903
[42]	Chuckran Peter F, Fofanov V, Hungate B A, et al. Rapid response of nitrogen cycling gene transcription to labile carbon amendments in a soil microbial community. mSystems, 2021, 6(3):e00161.
[43]	Subbarao G V, Ishikawa T, Ito O, et al. A bioluminescence assay to detect nitrification inhibitors released from plant roots:a case study with Brachiaria humidicola. Plant and Soil, 2006, 288(1/2):101-112. doi: 10.1007/s11104-006-9094-3
[44]	Subbarao G V, Wang H Y, Ito O, et al. NH₄⁺ triggers the synthesis and release of biological nitrification inhibition compounds in Brachiaria humidicola roots. Plant and Soil, 2007, 290(1):245-257. doi: 10.1007/s11104-006-9156-6
[45]	Leninger S, Urich T, Schloter M, et al. Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature, 2006, 442(17):806-809. doi: 10.1038/nature04983
[46]	Zakir H A K M, Subbarao G V, Pearse S J, et al. Detection,isolation and characterization of a root-exuded compound,methyl 3-(4-hydroxyphenyl) propionate,responsible for biological nitrification inhibition by sorghum (Sorghum bicolor). New Phytologist, 2008, 180(2):442-451. doi: 10.1111/nph.2008.180.issue-2
[47]	Zhu X M, Liu D Y, Yin H J. Roots regulate microbial N processes to achieve an efficient NH₄⁺ supply in the rhizosphere of alpine coniferous forests. Biogeochemistry, 2021, 12:1-19.
[48]	Boudsocq S, Lata J C, Mathieu J, et al. Modelling approach to analyses the effects of nitrification inhibition on primary production. Functional Ecology, 2009, 23(1):220-230. doi: 10.1111/fec.2009.23.issue-1
[49]	Frank D A, Groffman P M. Plant rhizosphere N processes:what we don’t know and why we should care. Ecology, 2009, 90(6):1512-1519. doi: 10.1890/08-0789.1
[50]	Subbarao G V, Kishii M, Nakahara K, et al. Biological nitrification inhibition (BNI)-is there potential for genetic interventions in the Triticeae?. Breeding Science, 2009, 59(5):529-545. doi: 10.1270/jsbbs.59.529
[51]	Thilakarathna S K, Hernandez-Ramirez G. How does management legacy, nitrogen addition, and nitrification inhibition affect soil organic matter priming and nitrous oxide production?. Journal of Environmental Quality, 2020, 50(1):78-93. doi: 10.1002/jeq2.v50.1
[52]	Raaijmakers J M, Paulitz T C, Steinberg C, et al. The rhizosphere:a playground and battle-field for soil borne pathogens and beneficial microorganisms. Plant and Soil, 2009, 321(1):341-361. doi: 10.1007/s11104-008-9568-6
[53]	Walker T S, Bais H P, Grotewold E, et al. Root exudation and rhizosphere biology. Plant Physiology, 2003, 132(1):44-51. doi: 10.1104/pp.102.019661
[54]	Rengel Z, Marschner P. Nutrient availability and management in the rhizosphere:exploiting genotypic differences. New Phytologist, 2005, 168(2):305-312. pmid: 16219070
[55]	Wattenburger C J, Gutknecht J, Zhang Q, et al. The rhizosphere and cropping system,but not arbuscular mycorrhizae,affect ammonia oxidizing archaea and bacteria abundances in two agricultural soils. Applied Soil Ecology, 2020, 151:103540. doi: 10.1016/j.apsoil.2020.103540
[56]	Lata J C, Degrange V, Raynaud X, et al. Grass populations control nitrification in savanna soils. Functional Ecology, 2004, 18(4):605-611. doi: 10.1111/fec.2004.18.issue-4
[57]	Lu Y F, Zhang X N, Jiang J F, et al. Effect of the biological nitrification inhibitor 1,9-decanediol on nitrification and ammonia oxidizers in three agricultural soils. Soil Biology and Biochemistry, 2019, 129:48-59. doi: 10.1016/j.soilbio.2018.11.008
[58]	Myrold D D, Tiedje J M. Establishment of denitrification capacity in soil:effects of carbon,nitrate and moisture. Soil Biology and Biochemistry, 1985, 17(6):819-822. doi: 10.1016/0038-0717(85)90140-3
[59]	张晓楠, 陆玉芳, 杨婷, 等. 水稻生物硝化抑制剂1,9,-癸二醇的定量方法优化. 土壤, 2020, 52(6):1152-1157.
[60]	Subbarao G V, Yoshashi T, Worthington M, et al. Suppression of soil nitrification by plants. Plant Science, 2015, 233(1):155-164. doi: 10.1016/j.plantsci.2015.01.012
[61]	Subbarao G V, Rondon M, Ito O, et al. Biological nitrification inhibition (BNI)- is it a widespread phenomenon?. Plant and Soil, 2007, 294(1):5-18. doi: 10.1007/s11104-006-9159-3
[62]	Subbarao G V, Ban T, Masahiro K, et al. Can biological nitrification inhibition (BNI) genes from perennial Leymus racemosus (Triticeae) combat nitrification in wheat farming?. Plant and Soil, 2007, 299(1):55-64. doi: 10.1007/s11104-007-9360-z
[63]	Subbarao G V, Nakahara K, Hurtado M P, et al. Evidence for biological nitrification inhibition in Brachiaria pastures. Proceedings of National Academy of Sciences of the United States of America, 2009, 106(41):17302-17307.
[64]	Subbarao G V, Nakahara K, Ishikawa T, et al. Free fatty acids from the pasture grass Brachiaria humidicola and one of their methyl esters as inhibitors of nitrification. Plant and Soil, 2008, 313(2):89-99. doi: 10.1007/s11104-008-9682-5
[65]	Coskun D, Britto D T, Shi W M, et al. Nitrogen transformations in modern agriculture and the role of biological nitrification inhibition. Nature Plants, 2017, 3(6):1-9.
[66]	Subbarao G V, Nakahara K, Ishikawa T, et al. Biological nitrification inhibition (BNI) activity in sorghum and its characterization. Plant and Soil, 2013, 366(1):243-259. doi: 10.1007/s11104-012-1419-9
[67]	Wolt J D. A meta-evaluation of nitrapyrin agronomic and environmental effectiveness with emphasis on corn production in the Mid-Western USA. Nutrient Cycling in Agroecosystems, 2004, 69(1):23-41. doi: 10.1023/B:FRES.0000025287.52565.99
[68]	Gopalakrishnan S, Watanabe T, Pearse S J, et al. Biological nitrification inhibition by Brachiaria humidicola roots varies with soil type and inhibits nitrifying bacteria,but not other major soil microorganisms. Soil Science and Plant Nutrition, 2009, 55(5):725-733. doi: 10.1111/j.1747-0765.2009.00398.x
[69]	Cooper A B. Suppression of nitrate formation with an exotic conifer plantation. Plant and Soil, 1986, 93(3):383-394. doi: 10.1007/BF02374289
[70]	Canfield D E, Glazer A N, Falkowski P G. The evolution and future of earth’s nitrogen cycle. Science, 2010, 330(4):192-196. doi: 10.1126/science.1186120
[71]	McCarty G W. Modes of action of nitrification inhibitors. Biology and Fertility of Soils, 1999, 29(1):1-9. doi: 10.1007/s003740050518
[72]	Weng B, Xie X Y, Yang J J, et al. Research on the nitrogen cycle in rhizosphere of Kandelia obovate under ammonium and nitrate addition. Marine Pollution Bulletin, 2013, 76(4):227-240. doi: 10.1016/j.marpolbul.2013.08.034
[73]	Shi S J, Richardson A E, O’Callaghan M, et al. Effects of selected root exudate components on soil bacterial communities. FEMS Microbiology Ecology, 2011, 77(3):600-610. doi: 10.1111/j.1574-6941.2011.01150.x
[74]	Bais H P, Weir T L, Perry L G, et al. The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology, 2006, 57(1):233-266. doi: 10.1146/arplant.2006.57.issue-1
[75]	Li B, Li Y Y, Wu H M, et al. Root exudates drive interspecific facilitation by enhancing nodulation and N₂ fixation. Proceedings of National Academy of Sciences, 2016, 113(23):6496-6501.
[76]	Jalonen R, Nygren P, Sierra J. Root exudates of a legume tree as a nitrogen source for a tropical fodder grass. Nutrition Cycling and Agroecosystem, 2009, 85(3):203-213.
[77]	Zhu Y H, Zhang S Z, Huang H L, et al. Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils. Journal of Environmental Sciences, 2009, 21(7):920-926. doi: 10.1016/S1001-0742(08)62362-1
[78]	Yuan H Z, Zhu Z K, Liu S L, et al. Microbial utilization of rice root exudates：¹³C labeling and PLFA composition. Biology and Fertility of Soils, 2016, 52(5):615-627. doi: 10.1007/s00374-016-1101-0
[79]	Yin H J, Wheeler E, Phillips R P. Root-induced changes in nutrient cycling in forests depend on exudation rates. Soil Biology and Biochemistry, 2014, 78(6):213-221. doi: 10.1016/j.soilbio.2014.07.022
[80]	Vranova V, Rejsek K, Skene K R, et al. Methods of collection of plant root exudates in relation to plant metabolism and purpose:a review. Journal of Plant Nutrition Soil Science, 2013, 176(2):175-199. doi: 10.1002/jpln.v176.2
[81]	Michalet S, Rohr J, Warshan D, et al. Phytochemical analysis of mature tree root exudates in situ and their role in shaping soil microbial communities in relation to tree N-acquisition strategy. Plant Physiology and Biochemistry, 2013, 72(3):169-177. doi: 10.1016/j.plaphy.2013.05.003
[82]	Suo B, Chen Q, Wu W X, et al. Chemotactic responses of Phytophthora sojae zoospores to amino acids and sugars in root exudates. Journal of General Plant Pathology, 2016, 82(2):142-148. doi: 10.1007/s10327-016-0651-1
[83]	Sun L, Lu Y F, Kronzucker H J, et al. Quantification and enzyme targets of fatty acid amides from duckweed root exudates involved in the stimulation of denitrification. Journal of Plant Physiology, 2016, 198(1):81-88. doi: 10.1016/j.jplph.2016.04.010
[84]	Li H, Yang X R, Weng B, et al. The phenological stage of rice growth determines anaerobic ammonium oxidation activity in rhizosphere soil. Soil Biology and Biochemistry, 2016, 100(1):59-65. doi: 10.1016/j.soilbio.2016.05.015
[85]	Roque-Malo S, Woo D K, Kumar P. Modeling the role of root exudation in critical zone nutrient dynamics. Water Resources Research, 2020, 56:1-23.

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根系分泌物成分 Root exudate component	植物 Plant	抑制途径 Inhibition pathway	参考文献 Reference
苯丙酯MHPP	玉凤花	AMO	[48]
亚油酸Linoleic acid	玉凤花	AMO和HR	[49]
亚麻酸Linolenic acid	高梁	AMO和HR	[52-53]
脂肪醇1,9-decanediol	水稻	AMO	[59-60]
脂肪酸化合物Sorgoleone	高粱	AMO和HR	[61]
樱花素Sakuranetin	高粱	AMO和HR	[62]
柠檬烯Limonene	西黄松	AMO	[63]

Effects of Root Exudates on Key Processes of Soil Nitrogen Cycling: A Review

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