Crops ›› 2023, Vol. 39 ›› Issue (5): 179-186.doi: 10.16035/j.issn.1001-7283.2023.05.026

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Effects of Nitrogen Fertilizer Management on Rice Root Growth and Development and Its Relationships with Nitrogen Fertilizer Uptake and Utilization

Hu Rui(), Hu Xiangyu, Fu Youqiang, Ye Qunhuan, Pan Junfeng, Liang Kaiming, Li Meijuan, Liu Yanzhuo, Zhong Xuhua()   

  1. Rice Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory, Guangzhou 510640, Guangdong, China
  • Received:2022-05-14 Revised:2022-06-12 Online:2023-10-15 Published:2023-10-16

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Abstract:

The “Three Controls” nutrient management technology (TC) of rice is an optimized nitrogen management fertilization technology. Field experiments were conducted with TC and farmers’ fertilization practice (FP) as treatments. Root morphological traits, root activity, nitrogen uptake, dry matter accumulation, and grain yield were investigated. Under TC treatment, the total nitrogen input was 180kg N/ha in the late season and 150kg N/ha in the early season, respectively. Under FP treatment, the total nitrogen input was 210kg N/ha in the late season and 180kg N/ha in the early season, respectively. TC nitrogen fertilizer was applied according to base fertilizer 40%, tillering fertilizer 20%, ear fertilizer 30%, grain fertilizer 10%. FP nitrogen fertilizer was applied according to the proportion of base fertilizer 30%, return fertilizer 20%, tillering fertilizer 30%, long coarse fertilizer (applied 18-20d after transplanting) 20%. The results showed that compared to FP, the nitrogen recovery efficiency of TC was increased by 25.9 and 15.2 percentage points in 2019 and 2020, respectively. The nitrogen agronomic efficiency was increased by 89.6% and 74.4% in 2019 and 2020, respectively. Compared to FP, the partial factor productivity of TC was increased by 30.8% and 38.3% in 2019 and 2020, respectively. The root length, root surface area and root volume at booting stage of TC were significantly higher than those of FP. Compared to FP, the root activities of TC at booting stage were increased by 41.6% and 13.6% in 2019 and 2020, respectively. The root-shoot ratio of TC at tillering stage was significantly higher than that of FP in 2019. Correlation analysis results indicated that nitrogen recovery efficiency was positively correlated with the root vigor at booting stage. Nitrogen agronomic efficiency was positively correlated with the root length and root dry weight at booting stage. Partial factor productivity of nitrogen was positively correlated with root surface area at booting stage. These results indicated that the enhanced root growth and root vigor during panicle development contribute to the improvement of nitrogen use efficiency under TC.

Key words: Rice, Nitrogen fertilizer management, “Three Controls” nutrient management technology, Nitrogen use efficiency, Root

Fig.1

Temperature and solar radiation during the whole growth period of rice in the experimental base"

Table 1

Effects of nitrogen fertilizer management on rice material production and rice yield kg/hm2"

年份
Year		 处理
Treatment		 干物质积累Dry matter accumulation 籽粒产量
Grain yield
穗分化始期Panicle initiation 抽穗期Heading 成熟期Maturity
2019 N0 2594b 8286b 10 737b 5506a
FP 4099a 12 514a 13 276ab 6236a
TC 3657ab 11 751ab 15 812a 6920a
2020 N0 860b 3372b 7883c 4269b
FP 2702a 7176a 11 657b 6452a
TC 2180a 6577a 13 264a 7429a
方差分析ANOVA
年份Year (Y) ** ** ** ns
处理Treatment (T) ** ** ** **
年份×处理Y×T ns ns ns ns

Fig.2

Effects of nitrogen fertilizer management on the growth rate of rice Different letters indicate significant difference at the 0.05 level, the same below"

Table 2

Effeccts of nitrogen fertilizer management on nitrogen uptake of rice kg/hm2"

年份
Year		 处理
Treatment		 吸氮量Nitrogen uptake
穗分化始期
Panicle initiation		 抽穗期
Heading		 成熟期
Maturity
2019 N0 51.7b 85.3b 100.2b
FP 120.3a 184.9a 170.1a
TC 100.9a 194.7a 206.7a
2020 N0 13.3b 32.9b 67.7c
FP 37.3a 60.4a 94.9b
TC 27.6a 68.4a 113.1a
方差分析ANOVA
年份Year (Y) ** ** **
处理Treatment (T) ** ** **
年份×处理Y×T * ** **

Fig.3

Effects of nitrogen fertilizer management on nitrogen uptake rate of rice"

Table 3

Effects of nitrogen fertilizer management on nitrogen use efficiency of rice"

年份
Year		 处理
Treatment		 RE
(%)		 AE
(kg/kg)		 PEP
(kg/kg)
2019 FP 33.3a 6.7b 34.7b
TC 59.2a 12.7a 45.4a
2020 FP 15.1a 12.1a 35.8a
TC 30.3a 21.1a 49.5a
方差分析ANOVA
年份Year (Y) ** * ns
处理Treatment (T) * ** **
年份×处理Y×T ns ns ns

Table 4

Effects of nitrogen fertilizer management on root morphology and root vigor of rice"

年份
Year		 处理
Treatment		 分蘖期Tillering stage 孕穗期Booting stage 抽穗期Heading stage
根长
Root
length
(cm)		 根干重
Root
dry
matter
(g)		 根系
表面
积
Root
surface
area
(cm2)		 根系
体积
Root
volume
(cm3)		 根长
Root
length
(cm)		 根干重
Root
dry
matter
(g)		 根系
表面
积
Root
surface
area
(cm2)		 根系
体积
Root
volume
(cm3)		 根系
活力
Root
vigor
[μg α-NA/
(g DW·h)]		 颖花
根活量
Spikelet-
root activity
[μg α-NA/
(spikelet·h)]		 伤流量
Xylem
sap
(g)		 颖花根活量
[g/(穗·h)]
Spikelet-root
activity
[g/(spikelet·h)]
2019 FP 18.7a 0.54a 558a 4.9a 22.2b 2.07a 1969a 25.8a 305a 4.31a 2.51a 1.43a
TC 21.3a 0.58a 594a 5.4a 27.5a 2.32a 2270a 30.9a 432a 6.60a 2.87a 1.58a
2020 FP 17.0a 0.28a 317a 2.5a 33.7a 2.58a 2072a 26.0a 147a 2.39a 7.09a 1.26a
TC 15.9a 0.24a 270b 2.2a 33.2a 2.68a 2237a 28.5a 167a 2.46a 7.02a 1.26a
方差分析ANOVA
年份Year (Y) * ** ** ** ** ** ns ns ** ** ** *
处理Treatment (T) ns ns ns ns ** ns * * ns ns ns ns
年份×处理Y×T ns ns ns ns ** ns ns ns ns ns ns ns

Fig.4

Effects of nitrogen fertilizer management on root-shoot ratio of rice"

Table 5

Correlation coefficients between root morphology or root vigor and nitrogen use efficiency (n=12)"

生育期
Growth stage		 指标
Index		 RE AE PFP
分蘖期
Tillering stage		 根冠比 -0.375 0.604* 0.296
根长 0.528 -0.406 -0.096
根干重 0.679* -0.509 -0.127
根系表面积 0.643* -0.508 -0.144
根系体积 0.654* -0.478 -0.110
孕穗期
Booting stage		 根冠比 -0.412 0.683* 0.375
根长 -0.349 0.656* 0.354
根干重 -0.255 0.723** 0.471
根系表面积 0.257 0.536 0.710**
根系体积 0.478 0.287 0.544
根系活力 0.637* -0.308 0.128
颖花根活量 0.590* -0.291 0.115
抽穗期
Heading stage		 伤流量 -0.529 0.469 0.166
颖花根活量 0.372 -0.411 -0.140
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