Crops ›› 2023, Vol. 39 ›› Issue (6): 41-46.doi: 10.16035/j.issn.1001-7283.2023.06.006

Previous Articles     Next Articles

Molecular Detection and Geographic Distribution of Stripe Rust Resistance Gene Loci in Yunnan Wheat Landraces

Chen Dan1(), Xiong Furong2(), Wu Shaoyun1, Bai Xiaodong3, Zhou Guoyan1, Wu Xiaoyang1, Cai Qing1()   

  1. 1Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southwest China, Ministry of Agriculture and Rural Affairs/Yunnan Key Laboratory of Agricultural Biotechnology/National Crop Germplasm Bank-Yunnan Branch,Kunming 650205, Yunnan, China
    2School of Biology and Chemistry, Pu'er University, Pu'er 665000, Yunnan,China
    3Wuding County Maojie Town Agricultural and Rural Service Center of Chuxiong Prefecture,Yunnan Province, Chuxiong 651607, Yunnan, China
  • Received:2022-07-25 Revised:2022-09-05 Online:2023-12-15 Published:2023-12-15

RichHTML

3

PDF (PC)

81

Abstract:

The Yunnan wheat germplasm resources stored in the Yunnan Province Crop Germplasm Resources Bank are rich and diverse, and is a precious genetic resource for modern wheat breeding and improvement. Evaluation of exploration of the superior resistance genes from them are crucial steps in the enhancement of current wheat breeding. This study used molecular markers which were closely linked or co-isolated with resistance genes of Yr5, Yr10 and Yr18 to detect the gene loci in 208 yunnan wheat landrace, to clarify the distribution of stripe rust resistance gene loci of Yunnan wheat landrace, and provide theoretical reference for the selection and breeding of stripe rust resistance resources. The results showed that, Yr10 and Yr18 resistance genes were widely distributed in Yunnan wheat landrace, and a total of 143 test materials carried the two resistance genes above, accounting for 68.75%. Among them, the distribution frequency of Yr10 was the highest, with 104 materials carrying target fragments, there were 39 materials containing Yr18, however, Yr5 was less distributed, with only 12, accounting for 5.77%. At the same time, the geographical distribution of three disease resistance genes were studied. It was found that three resistance genes could be detected simultaneously in Baoshan Longyang District, Changning, Weishan, Fengqing and Lancang of Yunnan province. We speculate that this region might be the concentrated distribution region of Yr5, Yr10 and Yr18 genes.

Key words: Wheat, Landrace, Stripe rust resistance gene, Geographic distribution

Table 1

Molecular markers for detection of wheat stripe rust resistance genes"

基因
Gene		 标记名称
Marker name		 引物序列
Primer sequence		 连锁距离
Linkage distance (cM)		 退火温度
Annealing temperature (℃)		 文献
Reference
Yr5
 S1320
 F：CAATAGTTAGGCAAATTACATCG
R：GCTCAGTCAAACCGCTACTTCT		 0.3、0.0
 60
 [12]
Yr10
 SC200
 F：CTGCAGAGTGACATCATACA
R：TCGAACTAGTAGATGCTGGC		 0.5
 57
 [13]
Yr18
 csLV34
 F：GTTGGTTAAGACTGGTGATGG
R：TGCTTGCTATTGCTGAATAGT		 0.4、2.5
 60
 [14]

Table 2

Distribution of Yr5, Yr10 and Yr18 resistance genes in Yunnan wheat landraces"

编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip
1 + + 54 + 107 + 160 +
2 + 55 + 108 + 161 +
3 + 56 + 109 + 162 +
4 + 57 + 110 + 163 +
5 + 58 + 111 + 164 +
6 + 59 + 112 + 165 +
7 + 60 + 113 166 +
8 + 61 + 114 + + 167 +
9 + 62 + 115 + + 168 +
10 + + 63 + 116 + 169 +
11 + 64 + 117 + + 170 +
12 + 65 + 118 + 171 +
13 + 66 + 119 + 172 +
14 + 67 + 120 + 173 +
15 + 68 + 121 + + 174 +
16 + 69 + 122 + 175 +
17 + 70 + 123 + 176 +
18 + 71 + 124 + 177 +
19 + 72 + 125 + + 178 +
20 + 73 + 126 + 179 +
21 + 74 + 127 + 180 +
22 + 75 + 128 + 181 +
23 + 76 + 129 + 182 +
24 + 77 + 130 + 183 +
25 + 78 + 131 + 184 + +
26 + 79 + 132 + 185 + +
27 + 80 + 133 + + 186 +
28 + 81 + 134 + 187 +
29 + 82 + + 135 + 188 +
30 + 83 + 136 + 189 +
31 + 84 + 137 + 190 +
32 + 85 + 138 + + 191 +
33 + 86 + 139 + 192 +
34 + 87 + 140 + + 193 +
35 + 88 + 141 + 194 +
36 + 89 + 142 + 195 +
37 + 90 + 143 + 196 +
38 + 91 + 144 + 197 +
39 + 92 + 145 + 198 +
40 + 93 + + + 146 + 199 +
41 + 94 + 147 + 200 +
42 + 95 + 148 + 201 +
43 + 96 + 149 + 202 +
44 + 97 + 150 + 203 +
45 + 98 + 151 + 204 +
46 + 99 + 152 + 205 +
47 + 100 + 153 + 206 +
48 + 101 + 154 + 207 +
49 + 102 + 155 + 208 +
50 103 + 156 + Avocet S - - -
51 + + 104 + 157 + 阳性对照
Positive control		 + + +
52 + 105 + 158 +
53 + 106 + 159 +

Table 3

Distribution proportion of Yr5, Yr10 and Yr18 resistance genes in Yunnan wheat landraces"

项目Item Yr5 Yr10 Yr18 Yr18+Yr10 Yr18+Yr5 Yr10+Yr5 Yr18+Y10+Yr5 无带No strip
携带目标条带品种数量
Number of landraces with target bands		 12 104 39 5 6 3 1 67
携带目标条带品种占比
Percentage of the landraces with target bands (%)		 5.77 50.00 18.75 2.40 2.88 1.44 0.48 32.21

Fig.1

Detection results of molecular markers of Yr5, Yr10 and Yr18 resistance genes M: DL2000 marker; (a) 1-22 are part of samples, 23-24 are negative control Avocet S, 25 is positive control Avocet S*6/Yr5; (b) 1-28 are part of samples, 29-30 are negative control Avocet S, 31 is positive control Avocet S*6/Yr10; (c) 1-26 are part of samples, 27 is negative control Avocet S, 28-29 are positive control Avocet S*6/Yr18"

Table 4

Statistics on the number of Yunnan wheat landraces carrying resistance genes"

收集地
Select place		 Yr5 Yr10 Yr18 Yr18+
Yr10		 Yr10+
Yr5		 Yr18+
Yr5		 Yr18+
Yr10+Yr5
保山市隆阳区
Longyang District, Baoshan		 1 7 4 0 0 1 0
昌宁Changning 1 1 3 0 0 0 1
龙陵Longling 1 1 0 0 0 0 0
腾冲Tengchong 0 1 0 0 0 0 0
宾川Binchuan 0 0 0 0 0 0 0
剑川Jianchuan 0 0 1 0 0 0 0
南涧Nanjian 0 0 0 0 0 0 0
巍山Weishan 2 3 2 1 1 1 0
云龙Yunlong 0 0 1 0 0 0 0
德钦Deqin 0 0 0 0 0 0 0
维西Weixi 0 5 1 1 0 0 0
香格里拉
Shangri-La		 1 0 2 0 0 1 0
石屏Shiping 0 0 0 0 0 0 0
晋宁Jinning 0 1 2 1 0 0 0
永胜Yongsheng 0 1 0 0 0 0 0
凤庆Fengqing 1 24 8 0 0 1 0
耿马Gengma 0 2 0 0 0 0 0
临沧Lincang 0 4 2 0 0 0 0
双江Shuangjiang 0 8 0 0 0 0 0
永德Yongde 3 20 0 0 2 0 0
云县Yunxian 0 9 4 0 0 0 0
镇康Zhenkang 0 9 1 1 0 0 0
贡山Gongshan 0 1 0 0 0 0 0
兰坪Lanping 1 0 1 0 0 1 0
澜沧Lancang 1 6 4 0 0 1 0
普洱Pu?er 0 1 2 1 0 0 0
曲靖市麒麟区
Qilin District, Qujing		 0 0 0 0 0 0 0
玉溪红塔区
Hongta District, Yuxi		 0 0 1 0 0 0 0
[1] Wellings C R. Global status of stripe rust: a review of historical and current threats. Euphytica, 2011, 179(1):129-141.
doi: 10.1007/s10681-011-0360-y
[2] 康振生, 王晓杰, 赵杰, 等. 小麦条锈菌致病性及其变异研究进展. 中国农业科学, 2015, 48(17):3439-3453.
doi: 10.3864/j.issn.0578-1752.2015.17.011
[3] 吕建平, 卢俊, 何云松, 等. 云南省小麦条锈病发生流行原因及其治理对策初探. 中国农学通报, 2004, 20(3):232-234.
[4] 吴立人, 牛永春. 我国小麦条锈病持续控制的策略. 中国农业科学, 2000, 33(5):46-53.
doi: 10.3864/j.issn.0578-1752.2000-33-5-48-55
[5] Li J B, Dundas L, Dong C M, et al. Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. Theoretical and Applied Genetics, 2020, 133:1095-1107.
doi: 10.1007/s00122-020-03534-y
[6] 韩德俊, 康振生. 中国小麦品种抗条锈病现状及存在问题与对策. 植物保护, 2018, 44(5):1-12.
[7] 徐默然, 蔺瑞明, 王凤涛, 等. 103份小麦品种(系)抗条锈性和遗传多样性评价及基因检测. 中国农业科学, 2020, 53(4):748-760.
doi: 10.3864/j.issn.0578-1752.2020.04.007
[8] 陈向东, 吴晓军, 胡铁柱, 等. 国内外小麦种质抗条锈病评价及相关基因分子检测. 河南农业科学, 2019, 48(9):103-110.
[9] 董娜, 胡海燕, 胡铁柱, 等. 348份小麦种质中抗条锈病基因Yr5、Yr10和Yr18的分子标记检测与分布. 西北农业学报, 2019, 28(12):1960-1968.
[10] 尉法刚, 王光浩, 王长有, 等. 400份小麦品种(系)条锈病成株期抗性鉴定与评价. 植物遗传资源学报, 2020, 21(4):846-854.
[11] 张晓祥, 王玲, 寿路路. 改良CTAB方法快速提取小麦基因组DNA. 农业科学与技术, 2013, 14(7):946-949.
[12] 陈晓红, 牛永春, 胡宝忠. 用变性PAGE-银染法鉴定小麦抗条锈病基因Yr5的RAPD标记. 遗传学报, 2004, 31(3):270-274.
[13] 邵映田, 牛永春, 朱立煌, 等. 小麦抗条锈病基因Yr10的AFLP标记. 科学通报, 2001, 46(8):669-672.
[14] Lagudah E S, McFadden H, Singh R P, et al. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics, 2006, 114(1):21-30.
doi: 10.1007/s00122-006-0406-z pmid: 17008991
[15] 李明菊, 伍少云. 云南小麦品质改良亲缘材料成株期抗条锈性评价. 麦类作物学报, 2006, 26(1):113-116.
[16] 黄锦, 王志伟, 于亚雄, 等. 云南铁壳麦及其他小麦种质中慢锈基因Lr34/Yr18的分子检测. 西南农业学报, 2012, 25(4):1138-1142.
[17] 杨金华, 黄锦, 王志伟, 等. 云南铁壳麦对条锈病主要流行小种的抗性鉴定及遗传分析. 西南农业学报, 2012, 25(6):2074-2077.
[18] 李菁, 姚方杰, 龙黎, 等. 中国特有的3个普通小麦亚种抗条锈病评价及其抗性基因分子检测. 植物病理学报, 2020, 50 (4):426-441.
[19] 习玲, 王昱琦, 杨修, 等. 243份云南普通小麦地方品种抗条锈病鉴定及分子标记检测. 中国农业科学, 2021, 54(4):684-695.
doi: 10.3864/j.issn.0578-1752.2021.04.002
[1] Zhang Rong, Jiang Enxi, Chen Si, Yu Xurun, Chen Gang, Ran Liping, Xiong Fei. Study on the Grain Formation in Wheat Spike Regulated by Ethephon and 1-Methylcyclopropene [J]. Crops, 2023, 39(6): 101-107.
[2] Liu Zhewen, Guo Dandan, Chang Xuhong, Wang Demei, Wang Yanjie, Yang Yushuang, Liu Xiwei, Wang Yujiao, Shi Shubing, Zhao Guangcai. Response of Nitrogen Accumulation and Translocation after Anthesis in Strong Gluten Wheat to Nitrogen Topdressing Period and Proportion [J]. Crops, 2023, 39(6): 114-120.
[3] Liu Xiwei, Wang Demei, Wang Yanjie, Yang Yushuang, Zhao Guangcai, Chang Xuhong. Impacts Mechanism of Drought and Heat Stress in the Middle and Late Growing Period on Wheat Grain Yield Formation Process and Mitigation Measures [J]. Crops, 2023, 39(6): 17-25.
[4] Wang Yifan, Ren Ning, Dong Xiangyang, Zhao Yanan, Ye Youliang, Wang Yang, Huang Yufang. Effects of Controlled-Release and Ordinary Urea on Wheat Yield, Nitrogen Absorption and Economic Benefit [J]. Crops, 2023, 39(5): 117-123.
[5] Yang Mei, Yang Weijun, Gao Wencui, Jia Yonghong, Zhang Jinshan. Effects of Combined Application of Biochar and Nitrogen Fertilizer on Dry Matter Transport, Agronomic Characteristics and Yield of Winter Wheat in Irrigation Area [J]. Crops, 2023, 39(5): 138-144.
[6] Huang Jie, Ge Changbin, Wang Jun, Cao Yanyan, Qiao Jiliang, Liao Pingʼan, Song Danyang, Lu Wenying. Simulation Model of Relative Meteorological 1000-Grain Weight of Wheat of Luohe Based on Principal Component Regression [J]. Crops, 2023, 39(5): 212-218.
[7] Liu Shuhan, Chen Lei, Zhang Jianchao, Hu Gan, Sun Junyan, Liu Dongtao, Wang Junwei. Gene Differential Expression Analysis of TMS5 in the Fertility Conversion of Wheat BNS Sterile Line [J]. Crops, 2023, 39(5): 24-29.
[8] Zhang Dongxu, Hu Danzhu, Yan Jinlong, Feng Liyun, Wu Zhiyuan, Zhang Junling, Li Yanhua. Effects of Spraying Streptomyces on Yield and Photosynthetic Characteristics of Late-Sown Wheat under Different Crop Rotations [J]. Crops, 2023, 39(5): 255-263.
[9] Song Guicheng, Yu Guihong, Zhang Peng, Ma Hongxiang. Evaluation on Waterlogging Resistance of Different Wheat Varieties (Lines) at Jointing Stage [J]. Crops, 2023, 39(5): 30-36.
[10] Ge Changbin, Qin Suyan, Qiao Jiliang, Wang Jun, Qi Shuangli, Lu Wenying, Zhang Zhenyong. Comparative Analysis of Agronomic Traits, Quality and Disease Evolution of Approved Wheat Varieties in Southern Henan and Southern Huai River in Jiangsu from 2001 to 2021 [J]. Crops, 2023, 39(5): 49-58.
[11] Yang Cheng, Zhang Deqi, Du Simeng, Zhang Lijia, Jin Haiyang, Li Ying, Shao Yunhui, Wang Hanfang, Fang Baoting, Li Xiangdong, Liu Meijun. Effects of Dark and Strong Light Dehydration on the Photosystem Activity in Wheat Leaves in Vitro [J]. Crops, 2023, 39(5): 98-103.
[12] Zhang Mingwei, Ding Jinfeng, Zhu Xinkai, Guo Wenshan. Analysis of High-Yielding Planting Density and Nitrogen Application in Super-Late Sowing Wheat Following Rice [J]. Crops, 2023, 39(4): 126-135.
[13] Song Xiao, Zhang Keke, Yue Ke, Huang Chenchen, Huang Shaomin, Sun Jianguo, Guo Tengfei, Guo Doudou, Zhang Shuiqing, Pei Minnan. Differences of Enzyme Activities and Bacterial Communities in Rhizosphere Soil of Wheat Varieties with Different Nitrogen Efficiency [J]. Crops, 2023, 39(4): 188-194.
[14] Fu Xiaoyi, Wang Hongguang, Liu Zhilian, Li Dongxiao, He Mingqi, Li Ruiqi. Effects of Water Stress on Growth of Different Wheat Varieties at Seedling Stage and Selection of Drought Resistant Varieties [J]. Crops, 2023, 39(4): 224-229.
[15] Chen Yuanyuan, Li Guangsheng, Liu Yang, He Yuqi, Zhou Meiliang, Fang Zhengwu. Molecular Cloning and Functional Identification of Resistance Gene FtTIR of Tartary Buckwheat to Blight [J]. Crops, 2023, 39(4): 44-51.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!