Crops ›› 2023, Vol. 39 ›› Issue (5): 98-103.doi: 10.16035/j.issn.1001-7283.2023.05.014

Previous Articles     Next Articles

Effects of Dark and Strong Light Dehydration on the Photosystem Activity in Wheat Leaves in Vitro

Yang Cheng1(), Zhang Deqi1(), Du Simeng1, Zhang Lijia2, Jin Haiyang1, Li Ying1, Shao Yunhui1, Wang Hanfang1, Fang Baoting1, Li Xiangdong1(), Liu Meijun2()   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences/Henan Engineering Research Center for Wheat Yield-Quality Simultaneous Improvement/Zhengzhou University Graduate Training Base/Scientific Observing and Experimental Station of Crop Cultivation in Central Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory of Wheat Biology, Zhengzhou 450002, Henan, China
    2College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • Received:2022-03-29 Revised:2022-04-06 Online:2023-10-15 Published:2023-10-16

RichHTML

2

PDF (PC)

103

Abstract:

Dehydration is the natural process of wheat leaves under drought conditions. In order to explore the mechanism of dark and strong light dehydration on photosynthetic mechanism and their differences in detached wheat leaves, the effects of dehydration with dark and strong light on the activity of wheat photosystem were determined and analyzed by using chlorophyll fluorescence dynamics technology. The results showed that the strong light accelerated the leaf dehydration process. Under the condition of dark dehydration, the maximum photochemical efficiency did not decrease significantly with the increase of dehydration degree, but decreased significantly under strong light, and the decline rate gradually accelerated with the increase of dehydration degree. Under the condition of strong light dehydration, the electron transfer from QA to QB was inhibited, and the degree of inhibition was greater than that of dark dehydration. There was no significant change in photosystem II (PSII) donor side activity under either dark or strong light dehydration conditions. The coupling degree between PSII units did not change significantly under dark conditions, but decreased significantly under strong light dehydration. Finally, it showed that the decline of PSII activity and photosynthetic performance caused by dehydration depended on light, under strong light, with the increase of dehydration degree, the absorption and transfer activity of PSII to light energy and the degree of energy coupling between PSII units gradually decline, while under dark conditions, only severe dehydration will lead to the maximum photochemical efficiency, the number of reaction centers and the electron transfer activity from QA to QB decreased slightly.

Key words: Wheat, Dehydration, Drought, Photosynthesis, Photosystem II

Table 1

Parameters and formulas of rapid chlorophyll fluorescence inducing kinetic curve"

参数Parameter 计算方法Method of calculation
FM 暗适应后照光获得的最大荧光强度
FO 叶绿素荧光诱导动力学曲线20μs的荧光强度
Fv Fv=FM?FO
Ft t时的荧光强度F
FK K点（0.3ms）的荧光强度
FJ J点（3ms）的荧光强度
FI I点（30ms）的荧光强度
VJ VJ=(FJ?FO)/(FM?FO)
VI VI=(FI ?FO)/(FM ?FO)
MO MO=4(F300μs?FO)/(FM?FO)
ΨO ψO=1?VJ
φPO φPO=Fv/FM=(FM?FO)/FM
φEO φEo=ETo/ABS=[1?(FO/FM)]?ψO
φDO φDO=1?φPO
φRO φRO=φPO×(1?VI)
σRO σRO=(1?VI)/(1?VJ)
ABS/RC ABS/RC=MO?(1/ VJ)?(1/φPO)
RC/CSm RC/CSm=φPO×(VJ/MO)×FM
PIABS PIABS=(RC/ABS)×[φPO/(1?φPO)]×[ψO/(1?ψO)]

Fig.1

RWCs of wheat leaves at different times under strong light and dark dehydration"

Fig.2

OJIP curves of wheat leaves at different times under dark and strong light dehydration"

Fig.3

O-J part of OJIP curves of wheat leaves at different times under dark and strong light dehydration"

Fig.4

Effects of dark and strong light dehydration on O-K part of OJIP curves of wheat leaves at different times"

Fig.5

Changes of fluorescence parameters in wheat leaves with different RWC under dark and strong light dehydration treatments"

[1] 杨程, 张德奇, 杜思梦, 等. 黑暗诱导衰老对不同年代冬小麦品种旗叶光系统Ⅱ功能的影响. 应用生态学报, 2018, 29(8):2525-2531.
[2] Yang C, Zhang D, Li X, et al. Drought effects on photosynthetic performance of two wheat cultivars contrasting in drought. New Zealand Journal of Crop and Horticultural Science, 2021, 49(1):17-29.
doi: 10.1080/01140671.2020.1851264
[3] 陈新宜, 宋宇航, 张孟寒, 等. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用. 作物学报, 2022, 48(2):478-487.
doi: 10.3724/SP.J.1006.2022.11026
[4] 和娟, 唐燕, 李晓瑞, 等. 水分亏缺对小麦芒和旗叶光合特性及蔗糖、淀粉合成的影响. 干旱地区农业研究, 2021, 39(6):53-61,78.
[5] 孙爽, 杨晓光, 张镇涛, 等. 华北平原不同等级干旱对冬小麦产量的影响. 农业工程学报, 2021, 37(14):69-78.
[6] 胡阳阳, 卢红芳, 刘卫星, 等. 灌浆期高温与干旱胁迫对小麦籽粒淀粉合成关键酶活性及淀粉积累的影响. 作物学报, 2018, 44(4):591-600.
[7] 毛浩田, 陈梦莹, 吴楠, 等. 干旱胁迫对不同倍性小麦和八倍体小黑麦苗期光合能力与抗氧化系统的影响. 麦类作物学报, 2018, 38(10):1246-1254.
[8] Luisa C S M, Barbara L, Adriana B, et al. Antioxidant system in Boea hygroscopica: Changes in response to desiccation and red ration. Phytochemistry, 1994, 35(3):561-565.
doi: 10.1016/S0031-9422(00)90561-2
[9] 李鹏民, 高辉远,Reto J S. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用. 植物生理与分子生物学学报, 2005, 31(6):559-566.
[10] Rahma G A B C, Arafet M B, Walid D A B, et al. Comparative analysis of salt stress, duration and intensity, on the chloroplast ultrastructure and photosynthetic apparatus in Thellungiella salsuginea. Journal of Photochemistry and Photobiology,B:Biology, 2018, 183(6):275-287.
doi: 10.1016/j.jphotobiol.2018.04.047
[11] Yang C, Zhang Z, Gao H, et al. Mechanisms by which the infection of Sclerotinia sclerotiorum (Lib.) de Bary affects the photosynthetic performance in tobacco leaves. BMC Plant Biology, 2014, 14(1):240.
doi: 10.1186/s12870-014-0240-4
[12] Zhou R, Kan X, Chen J, et al. Drought-induced changes in photosynthetic electron transport in maize probed by prompt fluorescence, delayed fluorescenc, P700 and cyclic electron flow signals. Environmental and Experimental Botany, 2019, 158(2):51-62.
doi: 10.1016/j.envexpbot.2018.11.005
[13] Zeng F, Wang G, Liang Y, et al. Disentangling the photosynthesis performance in japonica rice during natural leaf senescence using OJIP fluorescence transient analysis. Functional Plant Biology, 2021, 48(2):206-217.
doi: 10.1071/FP20104 pmid: 33099327
[14] Jin L, Che X, Zhang Z, et al. The mechanisms by which phenanthrene affects the photosynthetic apparatus of cucumber leaves. Chemosphere, 2017, 168(2):1498-1505.
doi: 10.1016/j.chemosphere.2016.12.002
[15] Badr A, Brüggemann W. Comparative analysis of drought stress response of maize genotypes using chlorophyll fluorescence measurements and leaf relative water content. Photosynthetica. 2020, 58:638-645.
doi: 10.32615/ps.2020.014
[16] Chen S, Yang J, Zhang M, et al. Classification and characteristics of heat tolerance in Ageratina adenophora populations using fast chlorophyll a fluorescence rise O-J-I-P. Environmental and Experimental Botany, 2016, 122(2):126-140.
doi: 10.1016/j.envexpbot.2015.09.011
[17] Li P, Ma F. Different effects of light irradiation on the photosynthetic electron transport chain during apple tree leaf dehydration. Plant Physiology and Biochemistry, 2012, 55:16-22.
doi: 10.1016/j.plaphy.2012.03.007 pmid: 22484842
[18] Mihaljevi I, Lepedu H, Imi D, et al. Photochemical efficiency of photosystem II in two apple cultivars affected by elevated temperature and excess light in vivo. South African Journal of Botany, 2020, 130:316-326.
doi: 10.1016/j.sajb.2020.01.017
[19] Mathur S, Jajoo A, Mehta P, et al. Analysis of elevated temperature-induced inhibition of photosystem II using chlorophyll a fluorescence induction kinetics in wheat leaves (Triticum aestivum). Plant Biology, 2010, 13(1):1-6.
[20] 金立桥, 车兴凯, 张子山, 等. 高温、强光下黄瓜叶片PSII供体侧和受体侧的伤害程度与快速荧光参数Wk变化的关系. 植物生理学报, 2015, 51(6):969-976.
[1] Wang Yifan, Ren Ning, Dong Xiangyang, Zhao Yanan, Ye Youliang, Wang Yang, Huang Yufang. Effects of Controlled-Release and Ordinary Urea on Wheat Yield, Nitrogen Absorption and Economic Benefit [J]. Crops, 2023, 39(5): 117-123.
[2] Yang Mei, Yang Weijun, Gao Wencui, Jia Yonghong, Zhang Jinshan. Effects of Combined Application of Biochar and Nitrogen Fertilizer on Dry Matter Transport, Agronomic Characteristics and Yield of Winter Wheat in Irrigation Area [J]. Crops, 2023, 39(5): 138-144.
[3] Huang Jie, Ge Changbin, Wang Jun, Cao Yanyan, Qiao Jiliang, Liao Pingʼan, Song Danyang, Lu Wenying. Simulation Model of Relative Meteorological 1000-Grain Weight of Wheat of Luohe Based on Principal Component Regression [J]. Crops, 2023, 39(5): 212-218.
[4] Liu Hui, Long Xueyi, Jiao Yan, Wang Lihong. Effects of Combined Application of Biochar and Phosphate Fertilizer on Rice Growth and Yield [J]. Crops, 2023, 39(5): 238-248.
[5] Liu Shuhan, Chen Lei, Zhang Jianchao, Hu Gan, Sun Junyan, Liu Dongtao, Wang Junwei. Gene Differential Expression Analysis of TMS5 in the Fertility Conversion of Wheat BNS Sterile Line [J]. Crops, 2023, 39(5): 24-29.
[6] Zhang Dongxu, Hu Danzhu, Yan Jinlong, Feng Liyun, Wu Zhiyuan, Zhang Junling, Li Yanhua. Effects of Spraying Streptomyces on Yield and Photosynthetic Characteristics of Late-Sown Wheat under Different Crop Rotations [J]. Crops, 2023, 39(5): 255-263.
[7] Song Guicheng, Yu Guihong, Zhang Peng, Ma Hongxiang. Evaluation on Waterlogging Resistance of Different Wheat Varieties (Lines) at Jointing Stage [J]. Crops, 2023, 39(5): 30-36.
[8] Ge Changbin, Qin Suyan, Qiao Jiliang, Wang Jun, Qi Shuangli, Lu Wenying, Zhang Zhenyong. Comparative Analysis of Agronomic Traits, Quality and Disease Evolution of Approved Wheat Varieties in Southern Henan and Southern Huai River in Jiangsu from 2001 to 2021 [J]. Crops, 2023, 39(5): 49-58.
[9] Zhang Mingwei, Ding Jinfeng, Zhu Xinkai, Guo Wenshan. Analysis of High-Yielding Planting Density and Nitrogen Application in Super-Late Sowing Wheat Following Rice [J]. Crops, 2023, 39(4): 126-135.
[10] Song Xiao, Zhang Keke, Yue Ke, Huang Chenchen, Huang Shaomin, Sun Jianguo, Guo Tengfei, Guo Doudou, Zhang Shuiqing, Pei Minnan. Differences of Enzyme Activities and Bacterial Communities in Rhizosphere Soil of Wheat Varieties with Different Nitrogen Efficiency [J]. Crops, 2023, 39(4): 188-194.
[11] Jiang Shan, Liu Jia, Cao Liang, Ren Chunyuan, Jin Xijun, Zhang Yuxian. Effects of Exogenous Melatonin on Growth and Yield of Adzuki Bean under Drought Stress at Seedling Stage [J]. Crops, 2023, 39(4): 202-209.
[12] Fu Xiaoyi, Wang Hongguang, Liu Zhilian, Li Dongxiao, He Mingqi, Li Ruiqi. Effects of Water Stress on Growth of Different Wheat Varieties at Seedling Stage and Selection of Drought Resistant Varieties [J]. Crops, 2023, 39(4): 224-229.
[13] Chen Yuanyuan, Li Guangsheng, Liu Yang, He Yuqi, Zhou Meiliang, Fang Zhengwu. Molecular Cloning and Functional Identification of Resistance Gene FtTIR of Tartary Buckwheat to Blight [J]. Crops, 2023, 39(4): 44-51.
[14] Liu Ying, Gu Yunyi, Zhang Weiyang, Yang Jianchang. Research Advances in the Effects of Water and Nitrogen and Their Interaction on the Grain Yield, Water and Nitrogen Use Efficiencies of Wheat [J]. Crops, 2023, 39(4): 7-15.
[15] Li Hongsheng, Li Shaoxiang, Yang Zhonghui, Yang Jiali, Liu Kun, Xiong Shian, Li Fuqian, Guo Hui, Yang Mujun. Comparison ofPhenotype and Marker Detection in Seed Purity of Thermo-Photo Sensitive Two-Line WheatHybrids [J]. Crops, 2023, 39(4): 71-76.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!