Crops ›› 2024, Vol. 40 ›› Issue (2): 71-79.doi: 10.16035/j.issn.1001-7283.2024.02.009

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Effects of Different Concentrations of Exogenous Amino Acids on Growth and Related Physiological Indicators of Rice Seedlings

Liu Fanchao1,2(), Fang Shumei1,2, Wang Qingyan1,2, Wang Hanxin1, Niu Juanjuan1,2, Liang Xilong1,2()   

  1. 1College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2Heilongjiang Plant Growth Regulator Engineering Technology Research Center, Daqing 163319, Heilongjiang, China
  • Received:2023-03-24 Revised:2023-04-13 Online:2024-04-15 Published:2024-04-15

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Abstract:

With the test material of Kenjing 8, the effects of different exogenous amino acids (L-leucine, L-aspartic acid, L-glutamic acid, L-arginine) on rice seedlings growth and related physiological indicators were studied through greenhouse experiment. The results showed that, the addition of exogenous leucine (4 g/L), aspartic acid (1.00 g/L) and glutamic acid (0.9 g/L) could increase the accumulation of dry matter content, chlorophyll content, osmotic substance content, increase antioxidant enzyme activities and reduce malondialdehyde content, further promote root growth of seedlings, and improve the quality of seedlings. Specifically, the plant height of seedlings increased by 55.14%, 57.25% and 37.90%, respectively, the stem base width increased by 10.53%, 12.78% and 42.85%, respectively, the total dry matter mass increased by 66.91%, 42.75% and 38.66%, respectively, and the total chlorophyll content increased by 64.41%, 20.85% and 1.84%, respectively. The exogenous arginine mixed soil treatment showed that the root growth and underground dry matter accumulation of seedlings were significantly inhibited in the concentration range of seedlings. This study showed that exogenous leucine, aspartic acid and glutamic acid had significant regulatory effects. In particular, 4 g/L exogenous leucine had an outstanding effect, which could be used as a technical means to control seedling growth in production of cultivating high-quality rice seedlings.

Key words: Amino acid, Rice, Seedling quality, Agronomic traits, Resistance

Table 1

Experimental design scheme"

组别
Group		 处理
Treatment		 浓度
Concentration (g/L)
亮氨酸Leucine L1 1
L2 2
L3 4
L4 8
天门冬氨酸Aspartic acid T1 0.25
T2 0.50
T3 1.00
T4 5.00
谷氨酸Glutamic acid G1 0.9
G2 1.8
G3 3.6
G4 7.2
精氨酸Arginine J1 0.85
J2 1.70
J3 3.40
J4 10.20
对照Control CK 0.00

Table 2

Effects of different amino acid treatments on agronomic traits of rice seedlings"

组别
Group		 处理
Treatment		 株高
Plant
height
(cm)		 茎基宽
Stem base
width
(mm)		 叶面积
Leaf
area
(cm2)		 地上部
干物质量
Above ground
dry weight
(mg)		 地下部
干物质量
Underground
dry weight
(mg)		 根系总长度
Total root
length
(cm)		 总根表面积
Total root
surface area
(cm2)		 总根体积
Total root
volume
(cm3)		 充实度
Fullness
(mg/cm)		 生长函数
Growth
function
(mg/d)		 根冠比
Root-
shoot
ratio
亮氨酸
Leucine
 CK 13.80±0.95b 1.33±0.06a 0.85±0.07b 6.09±0.14b 7.36±1.09b 80.98±3.69cd 7.07±0.31b 0.05±0.002b 0.44±0.03cd 0.75±0.07c 1.21±0.15a
L1 15.69±0.67b 1.39±0.08a 1.89±0.39a 7.35±0.48b 8.35±0.15b 88.07±2.41c 7.87±0.65b 0.05±0.008b 0.47±0.01c 0.82±0.04c 1.32±0.07a
L2 16.22±1.57b 1.39±0.17a 1.53±0.19a 9.32±1.39a 9.37±0.80b 142.26±9.59a 12.15±1.40a 0.08±0.015a 0.57±0.03a 0.98±0.12b 1.02±0.05ab
L3 21.41±0.96a 1.47±0.16a 1.70±0.25a 10.65±0.47a 11.88±0.72a 125.78±10.01b 11.07±1.33a 0.08±0.012a 0.50±0.01b 1.01±0.05b 1.12±0.02a
L4 21.11±3.30a 1.48±0.17a 1.65±0.20a 9.92±1.04a 7.51±0.45b 70.97±6.11d 6.99±0.37b 0.05±0.005b 0.47±0.02bc 1.21±0.09a 0.76±0.09b
天门冬
氨酸
Aspartic
acid		 CK 13.80±0.95c 1.33±0.06a 0.85±0.07c 6.09±0.14c 7.36±1.09ab 80.98±3.69b 7.07±0.31bc 0.05±0.002bc 0.44±0.03b 0.75±0.06b 1.21±0.15a
T1 18.77±2.34b 1.38±0.09a 1.75±0.10b 6.80±0.28c 5.88±0.34b 76.87±4.33b 6.55±0.20c 0.04±0.001c 0.36±0.03c 0.71±0.04b 0.86±0.02b
T2 15.37±1.36c 1.37±0.16a 2.07±0.31ab 6.87±0.29c 8.51±0.98a 86.47±7.48b 7.44±0.70b 0.05±0.003b 0.38±0.02c 0.80±0.07b 1.24±0.13a
T3 21.70±0.54a 1.50±0.11a 2.47±0.43a 9.83±0.64a 9.37±0.92a 107.23±8.89a 9.54±0.42a 0.07±0.004a 0.45±0.02b 1.01±0.08a 0.95±0.04ab
T4 14.65±1.64c 1.39±0.16a 1.67±0.30b 8.25±1.16b 6.96±0.66ab 60.03±5.95c 5.71±0.70d 0.04±0.006c 0.54±0.05a 0.85±0.10b 0.84±0.04b
谷氨酸
Glutamic
acid
 CK 13.80±0.95d 1.33±0.06b 0.85±0.07c 6.09±0.14c 7.36±1.09b 80.98±3.69b 7.07±0.31b 0.05±0.002a 0.44±0.03b 0.75±0.07c 1.21±0.15a
G1 19.03±0.92c 1.90±0.17a 1.73±0.15b 8.87±0.44b 9.78±1.07a 97.94±7.40a 7.73±0.20b 0.05±0.001a 0.47±0.01b 0.86±0.05bc 1.10±0.03a
G2 21.42±0.83b 1.66±0.04a 2.74±0.32a 9.11±0.53a 6.57±0.46b 53.93±2.99c 5.01±0.50c 0.04±0.006b 0.46±0.01b 0.91±0.07b 0.72±0.03b
G3 24.11±0.92a 1.87±0.10a 2.65±0.56a 9.38±0.78a 6.45±0.66b 58.42±2.81c 5.50±0.61c 0.04±0.008ab 0.39±0.02c 0.87±0.06bc 0.69±0.03b
G4 19.24±1.46c 1.79±0.18a 2.45±0.27a 10.96±0.79a 6.24±0.39b 46.15±3.39d 5.89±2.25a 0.04±0.005ab 0.56±0.03a 1.15±0.10a 0.57±0.05c
精氨酸
Arginine
 CK 13.80±0.95b 1.33±0.06cd 0.85±0.07c 6.09±0.14c 7.36±1.09a 80.98±3.69a 7.07±0.31a 0.05±0.002a 0.44±0.03c 0.75±0.07c 1.21±0.15a
J1 15.08±0.58b 1.61±0.03b 2.11±0.34b 7.54±0.44b 6.32±0.41ab 61.04±3.27b 5.69±0.45b 0.04±0.002a 0.50±0.04b 0.77±0.04bc 0.84±0.01b
J2 22.74±0.16a 1.82±0.04a 2.79±0.29a 11.97±0.69a 5.89±0.49bc 49.43±4.92c 4.81±0.28c 0.04±0.003a 0.53±0.03b 0.99±0.07a 0.49±0.02c
J3 20.32±2.73a 1.39±0.13c 3.22±0.13a 10.84±1.00a 4.89±0.53c 44.92±4.24c 4.41±0.61c 0.03±0.006a 0.53±0.03b 0.87±0.08b 0.45±0.04c
J4 10.78±1.15c 1.18±0.12d 1.04±0.32c 6.72±0.80bc 2.95±0.23d 21.01±2.60d 2.72±0.34d 0.01±0.001a 0.62±0.03a 0.54±0.05d 0.44±0.05c

Fig.1

Effects of different treatments on root vigor of rice seedlings Different lowercase letters indicates the significant difference among different treatments (P < 0.05), the same below."

Table 3

Effects of different treatments on chlorophyll contents in rice seedlings"

组别
Group		 处理
Treatment		 叶绿素a含量
Chlorophyll a content (mg/g)		 叶绿素b含量
Chlorophyll b content (mg/g)		 叶绿素总含量
Total chlorophyll content (mg/g)		 叶绿素a/b
Chlorophyll a/b
亮氨酸Leucine CK 1.30±0.02c 0.32±0.01c 1.63±0.03c 4.00±0.06a
L1 1.14±0.02c 0.32±0.01c 1.46±0.01c 3.55±0.16b
L2 1.79±0.01b 0.53±0.01b 2.32±0.01b 3.38±0.09bc
L3 2.04±0.41b 0.63±0.17b 2.68±0.59b 3.27±0.26c
L4 2.41±0.01a 0.86±0.01a 3.27±0.01a 2.79±0.02d
天门冬氨酸Aspartic acid CK 1.30±0.02b 0.32±0.01c 1.63±0.03b 4.00±0.06a
T1 1.14±0.11c 0.31±0.01c 1.46±0.13c 3.65±0.21b
T2 1.33±0.06b 0.37±0.03b 1.69±0.09b 3.63±0.11b
T3 1.54±0.06a 0.42±0.02a 1.97±0.08a 3.66±0.05b
T4 1.16±0.02c 0.32±0.01c 1.48±0.03c 3.69±1.10b
谷氨酸Glutamic acid CK 1.30±0.02bc 0.32±0.01c 1.63±0.03c 4.00±0.06a
G1 1.37±0.11bc 0.29±0.03c 1.66±0.14c 4.02±0.11a
G2 1.49±0.12b 0.41±0.05c 1.89±0.17b 3.66±0.17ab
G3 1.51±0.30b 0.56±0.14b 2.07±0.17b 2.91±1.11b
G4 2.20±0.03a 0.70±0.01a 2.90±0.03a 3.15±0.05ab
精氨酸Arginine CK 1.30±0.02d 0.32±0.01c 1.63±0.03d 4.00±0.06a
J1 0.70±0.04e 0.19±0.03d 0.89±0.07e 3.79±0.37ab
J2 2.10±0.01a 0.62±0.01a 2.72±0.01a 3.41±0.03b
J3 1.80±0.06c 0.52±0.02b 2.32±0.07c 3.48±0.08ab
J4 1.97±0.10b 0.59±0.04a 2.56±0.14b 3.32±0.08b

Fig.2

Effects of different treatments on carbon metabolite of rice seedlings"

Fig.3

Effects of different treatments on protective enzyme activities of rice seedlings"

Fig.4

Effects of different treatments on osmoregulation substances contents of rice seedlings"

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