Crops ›› 2024, Vol. 40 ›› Issue (2): 249-254.doi: 10.16035/j.issn.1001-7283.2024.02.031

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Germplasm Creation and New Variety Breeding for Fusarium Wilt Resistance in Mung Bean

Zhang Zhixiao(), Shen Yingchao, Fan Baojie, Wang Yan, Liu Changyou, Wang Shen, Cao Zhimin, Su Qiuzhu, Shi Huiying, Tian Jing()   

  1. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences /Hebei Crop Genetics and Breeding Laboratory, Shijiazhuang 050035, Hebei, China
  • Received:2022-12-27 Revised:2023-09-17 Online:2024-04-15 Published:2024-04-15

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Abstract:

Fusarium wilt is one of the most serious diseases affecting the yield of mung bean. Chlamydospores of the pathogen have strong resistance to stress and long survival duration in soil, so it is difficult to control them by agricultural and chemical methods. The most economic and effective method is to screen and create germplasm and breed new varieties with disease resistance. The 81 bred lines of mung bean were identified for their resistance to Fusarium wilt by root-cutting and root-soaking inoculation in seedling stage. The results showed that there were obvious resistant differences among the strains. Jilü 23, a new mung bean variety with high resistance to Fusarium wilt, high and stable yield and good quality, was bred through primary yield test, high yield appraisal test, provincial regional test and production test.

Key words: Mung bean, Fusarium wilt, Resistance, New variety

Fig.1

Phenotypes after 14 days of inoculation of disease-resistant and disease-susceptible materials of new strains of mung bean with F.oxysporum"

Table 1

Identification of resistance to Fusarium wilt in new mung bean lines at seedling stage"

序号
Number		 品系
Line		 病情指数
DI		 抗性评价
Resistance evaluation		 序号
Number		 品系
Line		 病情指数
DI		 抗性评价
Resistance evaluation
1 HN0913-6-6-1-4-4-1 4.47 HR 8 1105-12 18.02 R
2 HN0913-6-6-1-4-1-1 11.43 HR 9 HN0802-1-2-2-1-1-4 26.55 R
3 0914-13-1-2 4.93 HR 10 HN0913-6-10-3-2-1-3 29.50 R
4 1111反-1 5.91 HR 11 1015-4-1-1 31.59 R
5 HN1012-5-4-2 9.60 HR 12 1110反-3 33.10 R
6 HN1012-6-2-2 14.81 HR 13 1014反-2-3-2 33.47 R
7 1105-7 16.64 R 14 1109-8 34.72 R

Table 2

Agronomic traits and yield performance of resistant lines"

序号
Number		 品种（系）
Variety (line)		 生育期
Growth
period (d)		 株高
Plant height
(cm)		 单株分枝数
Branch number
per plant		 单株荚数
Pods
per plant		 单荚粒数
Seeds per
pod		 百粒重
100-seed
weight (g)		 产量
Yield
(kg/hm2)		 较对照增产率
Yield increase
than control (%)
1 HN0802-1-2-2-1-1-4 65 68.0 4.4 23.4 14.5 6.85 1554.00 23.75*
2 0914-13-1-2 65 74.0 3.2 18.9 10.1 6.63 1438.20 14.53*
3 1015-4-1-1 65 64.0 3.3 12.3 7.5 6.68 1212.60 -3.43
4 1111反-1 65 74.0 3.8 19.6 10.4 6.40 1209.00 -3.73
5 1105-7 65 70.0 3.9 16.3 8.6 6.42 1155.90 -7.96
6 1105-12 65 67.0 3.5 14.5 7.8 6.30 1126.95 -10.26
7 1110反-3 65 86.0 3.4 9.5 5.5 6.93 899.70 -28.36
8 冀绿7号(CK) 65 67.0 3.1 17.5 8.9 6.43 1255.80

Fig.2

Vascular bundle and plant phenotype of mung bean line materials at harvest stage in field disease nursery (a) Vascular bundle at stem base of susceptible line; (b) Plant phenotype of susceptible line at maturity; (c) Vascular bundle at stem base of resistant line; (d) Plant phenotype of resistant line at maturity."

Table 3

Multiple comparison results of yield difference of mung bean varieties (lines) in Hebei regional trial"

序号
Number		 品种（系）
Variety (line)		 产量
Yield
(kg/hm2)		 比对照增产
Yield increase
than control (%)
1 HN0802-1-2-2-1-1-4 1817.6a 9.80
2 保绿201321-7 1765.8ab 6.67
3 保绿942（CK） 1655.4bc 0.00
4 保绿201347-6 1631.2c -1.46
5 廊绿2号 1632.6c -1.38
6 廊绿1号 1621.6c -2.04
7 冀绿0911-1-2 1611.2c -2.67
8 冀绿抗1004-38-2-3-2-3 1569.6c -5.18
9 保绿201326-1 1565.4c -5.44

Table 4

Yield results in different sites of HN0802-1-2-2-1-1-4"

区域
Region		 试点
Site		 2019年平均产量
Average yield
in 2019 (kg/hm2)		 2019年增产率
Yield increase
in 2019 (%)		 2020年平均产量
Average yield in
2020 (kg/hm2)		 2020年增产率
Yield increase
in 2020 (%)		 2年平均产量
Average yield in
two years (kg/hm2)		 2年平均增产率
Average yield increase
in two years (%)		 位次
Ranking
夏播区
Summer sowing area		 石家庄 2078.00 12.26 1885.70 2.82 1981.90 7.57 1
保定 1985.00 0.40 1787.00 0.51 1886.00 0.45 5
唐山 1655.00 -2.42 1769.20 8.35 1712.10 2.86 3
廊坊 1157.00 3.58 1736.40 8.11 1446.70 6.25 1
衡水 553.00 -27.90 1750.00 7.51 1151.50 -3.83 6
邯郸 2740.00 3.01 1310.00 22.43 2025.00 8.58 1
平均Average 1694.70 -1.85 1706.38 8.29 1700.53 3.65 1
春播区
Spring sowing area		 张家口 1983.30 -16.00 2081.80 37.95 2032.60 5.04 1
阳原 2690.00 56.40 1920.00 50.00 2305.00 53.67 1
平均Average 2336.65 20.20 2000.90 43.98 2168.80 29.36 1

Table 5

Analysis of yield and stability of test varieties"

序号
Number		 品种（系）
Variety (line)		 丰产性参数Yield index 稳定性参数Stability index 适应地区
Suitable sites		 综合评价
Comprehensive evaluation
产量Yield (kg/hm2) 效应 方差 变异度
1 HN0802-1-2-2-1-1-4 1817.6 0.167 0.024 8.402 E1~E8 很好
2 保绿201321-7 1765.8 0.119 0.021 8.082 E1~E8 好
3 保绿942（CK） 1655.4 0.004 0.024 9.147 E1~E8 一般
4 保绿201347-6 1631.2 -0.022 0.023 9.094 E1~E8 一般
5 廊绿2号 1632.6 -0.023 0.010 6.138 E1~E8 一般
6 廊绿1号 1621.6 -0.033 0.009 5.663 E1~E8 一般
7 冀绿0911-1-2 1611.2 -0.042 0.061 15.177 E1~E8 一般
8 冀绿抗1004-38-2-3-2-3 1569.6 -0.083 0.028 10.459 E1~E8 较差
9 保绿201326-1 1565.4 -0.088 0.016 8.014 E1~E8 较差

Table 6

Agronomic characteristics of HN0802-1-2-2-1-1-4"

区域
Region		 生育期
Growth
period (d)		 株高
Plant height
(cm)		 单株分枝数
Branch number
per plant		 主茎节数
The number of main
stem sections		 单株荚数
Pod number
per plant		 荚长
Pod length
(cm)		 单荚粒数
Grain number
per pod		 百粒重
100-seed
weight (g)
春播区Spring sowing area 77.0 46.6 4.1 9.9 30.7 11.8 9.9 7.0
夏播区Summer sowing area 68.0 55.6 3.9 9.0 27.6 12.2 10.5 6.8

Table 7

Yield performance in production test of HN0802-1-2-2-1-1-4"

试点
Site		 产量
Yield
(kg/hm2)		 对照种产量
Yield of CK
(kg/hm2)		 较CK增产率
Yield increase
than CK (%)
石家庄Shijiazhuang 2508.00 1653.00 51.72
保定Baoding 1750.80 1434.90 22.02
唐山Tangshan 1794.00 1662.00 7.94
廊坊Langfang 1545.00 1509.00 2.39
张家口Zhangjiakou 2088.00 1566.00 33.33
平均Average 1937.10 1565.10 23.77
[1] 林汝法, 柴岩, 廖琴, 等. 中国小杂粮. 北京: 中国农业科学技术出版社, 2002.
[2] 程须珍. 绿豆生产技术. 北京: 北京出版集团公司, 2016.
[3] 左拴秀, 郭忠. 立足资源优势发展小杂粮产业. 中国农业资源与区划, 2005, 4(26):42-46.
[4] 梁双波, 程汝宏. 小杂粮在中国种质结构调整中的地位与发展策略. 河北农业科学, 2005, 9(2):93-95.
[5] Kelly L. Fusarium species associated with grain sorghum and mung bean in Queensland. Brisbane: The University of Queensland, 2018.
[6] Sun F F, Sun S L, Zhu L, et al. Confirmation of Fusarium oxysporum as acausal agent of mung bean wilt in China. Crop Protection, 2019, 117:77-85.
doi: 10.1016/j.cropro.2018.11.017
[7] 朱振东, 段灿星. 绿豆病虫害鉴定与防治手册. 北京: 中国农业科学技术出版社, 2012.
[8] Koike S T, Gordon T R. Management of Fusarium wilt of strawberry. Crop Protection, 2015, 73:67-72.
doi: 10.1016/j.cropro.2015.02.003
[9] Bennett R S, Spurgeon D W, DeTar W R, et al. Effcacy of four soil treatments against Fusarium oxysporum f. sp. vasinfectum race 4 on cotton. Plant Disease, 2011, 95(8):967-976.
doi: 10.1094/PDIS-09-10-0696 pmid: 30732107
[10] Dubey S C, Suresh M, Singh B. Evaluation of trichoderma species against Fusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biological Control, 2007, 40(1):118-127.
doi: 10.1016/j.biocontrol.2006.06.006
[11] Mansoor F, Sultana V, Ehteshamul-Haque S. Enhancement of biocontrol potential of Pseudomonas aeruginosa and Paecilomyces lilacinus against root rot of mung bean by a medicinal plant Launaea nudicaulis L.. Pakistan Journal of Botany, 2007, 39(6):2113-2119.
[12] Dhingra O D, Netto R A C. Reservoir and non-reservoir hosts of bean-wilt pathogen, Fusarium oxysporum f. sp. phaseoli. Journal of Phytopathology, 2008, 149(7/8):463-467.
[13] 张海斌, 蒙美莲, 刘坤雨, 等. 不同轮作模式对马铃薯干物质积累、病害发生及产量的影响. 作物杂志, 2019(4):170-175.
[14] 曾莉莎, 林威鹏, 吕顺, 等. 香蕉―甘蔗轮作模式防控香蕉枯萎病的持续效果与土壤微生态机理(Ⅰ). 中国生态农业学报, 2019, 27(2):257-266.
[15] 林威鹏, 曾莉莎, 吕顺, 等. 香蕉―甘蔗轮作模式防控香蕉枯萎病的持续效果与土壤微生态机理(Ⅱ). 中国生态农业学报, 2021, 27(3):348-357.
[16] 郭予元, 吴孔明. 中国农作物病虫害. 北京: 中国农业科学技术出版社, 2014:1001-1003.
[17] Nair R, Schafleitner R, Easdown W, et al. Legume improvement program at AVRDC-the world vegetable center: impact and future prospects. Ratarstvo i Povrtarstvo, 2014, 51(1):55-61.
doi: 10.5937/ratpov51-5488
[18] 杨宇红, 吕红豪, 杨翠荣, 等. 甘蓝枯萎病苗期抗性鉴定技术及抗源筛选. 植物保护学报, 2011, 38(5):425-431.
[19] 李园, 武晶. 普通菜豆镰孢菌枯萎病研究进展. 植物遗传资源学报, 2021, 22(3):603-608.
doi: 10.13430/j.cnki.jpgr.20200930001
[20] Cianchetta A N, Davis R M. Fusarium wilt of cotton: management strategies. Crop Protection, 2015, 73:40-44.
doi: 10.1016/j.cropro.2015.01.014
[21] 朱琳, 孙素丽, 孙菲菲, 等. 绿豆尖镰孢枯萎病抗性鉴定方法. 植物遗传资源学报, 2017, 18(4):696-703.
[22] Sun S L, Zhu L, Sun F F, et al. Pathotype diversity of Fusarium oxysporum f. sp. mungcola causing wilt on mungbean (Vigna radiata). Crop and Pasture Science, 2020, 71(10):873.
doi: 10.1071/CP20164
[23] Ulloa M, Hutmacher R B, Roberts P A, et al. Breeding for Fusarium wilt race 4 resistance in cotton under field and greenhouse conditions. Journal of Cotton Science, 2006, 10(2):114-127.
[24] Yuan S K, Zhou M G. A major gene for resistance to carbendazim, in field isolates of Gibberella zeae. Canadian Journal of Plant Pathology, 2005, 27(1):58-63.
doi: 10.1080/07060660509507194
[25] 孙文姬, 陈其焕, 马存, 等. 棉花种质资源抗枯、黄萎病鉴定. 中国农业科学, 1990, 23(1):89-90.
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