Crops ›› 2016, Vol. 32 ›› Issue (5): 31-37.doi: 10.16035/j.issn.1001-7283.2016.05.006

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QTL Mapping of Phenotypic Traits under Drought Stress Simulated by PEG-6000 in Rice Seedlings

Jiang Xue1,2,Ma Xiaosong2,Luo Lijun2,Liu Hongyan2   

  1. 1 Guizhou Rice Institute,Guiyang 550006,Guizhou,China
    2 Shanghai Agrobiological Gene Center,Shanghai 201106,China
  • Received:2016-07-21 Revised:2016-08-29 Online:2016-10-15 Published:2018-08-26
  • Contact: Hongyan Liu

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Abstract:

A recombination inbred line (RIL) population with 159 lines derived from Zhengshan97B (ZS97B) and IRAT109 were treated with normal water condition and 18% polyethylene glycol (PEG-6000). Five traits including seedling height, root length, seedling height growth rate, root length seedling height ratio and leaf rolling score were measured and further used for QTL analysis. A total of 24 QTLs were detected and each locus explained 7.35%-39.30% of phenotypic variance.13 QTLs were detected on chromosomes 1, 2, 3, 5, 6, 10 and 12 under normal water condition, while 11 QTLs on chromosomes 1, 3, 5, 7, 10 and 12 under drought stress were detected. The QTLs detected under two treatments showed significant differences, which there was different genetic mechanisms controlling rice phenotypes under two different conditions. Furthermore important QTLs for many seedling traits were detected between RM472 and RM104 on chromosome 1, and this interval contained QTL for seedling height under both two different water conditions simultaneously.

Key words: Rice, At seedling stage, Drought tolerance, QTL mapping, Phenotypic traits

Fig.1

The seedling height and root length of parents under normal and stress condition “**” represents the difference with significance of 1% levels between normal and stress condition"

Table 1

Performance of RIL population for seedling height and root length under stress and normal condition"

处理
Treatments		 性状Trait RIL群体RIL population
平均值Means 标准差Standard deviation 变异范围Range
正常条件Normal 苗高Plant height(cm) 23.63 4.34 12.44~33.89
根长Root length(cm) 18.62 2.82 9.85~25.39
模拟干旱Stress 苗高Plant height(cm) 18.62** 2.85 11.17~24.75
根长Root length(cm) 12.66** 2.03 5.24~18.19

Fig.2

Frequency distribution of RIL population"

Table 2

QTL for relative traits under normal and stress condition"

条件Conditions 性状Traits QTL Loci 标记区间Marker Interval 加性效应Add LOD 贡献率PVE(%)
正常条件Normal 苗高Seedling height qSH-1 RM472-RM104 0.93 8.77 34.31
qSH-3 RM16-RM426 0.72 3.36 17.74
qSH-5-1 RM163-RM459 -0.55 4.05 10.34
qSH-5-2 RM459-RM161 -0.68 6.73 17.16
最大根长Maximum root length qRL-1-1 RM302-RM476B 0.65 3.59 10.36
qRL-1-2 RM476B-RM315 0.61 3.91 10.70
qRL-12 RM247-RM512 0.75 3.99 14.63
苗高生长速率Seedling height growth rate qGRSH-1 RM472-RM104 0.13 5.43 21.16
qGRSH-2 RM526-RM525 0.10 4.41 12.74
根长苗高比Root length seedling height ratio qRS-3-1 RM231-RM489 0.05 3.11 7.35
qRS-3-2 RM489-RM545 0.05 3.24 8.75
qRS-6 RM528-RM30 0.06 3.63 8.75
qRS-10 RM222-RM216 -0.07 5.29 14.36
胁迫处理Stress 苗高Seedling height qSH-1 RM472-RM104 1.96 9.59 39.30
qSH-5-1 RM163-RM459 -0.92 4.38 11.41
qSH-5-2 RM459-RM161 -0.89 4.03 9.87
qSH-10 RM222-RM216 1.05 4.93 12.74
最大根长Maximum root length qRL-1-3 RM472-RM104 0.85 3.37 9.08
qRL-7 RM18-RM478 0.75 4.06 10.89
qRL-12 RM415-RM4A 0.95 4.12 20.27
根长苗高比Root length seedling height ratio qRS-1 RM472-RM104 -0.06 7.43 20.00
qRS-3-3 RM16-RM426 -0.07 3.22 19.53
qRS-7 RM478-RM134 0.05 4.13 10.38
叶卷曲Leaf rolling score qLRS-1 RM302-RM476B -0.95 3.06 10.74

Fig.3

QTL for seedling traits of the RIL population"

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