Crops ›› 2016, Vol. 32 ›› Issue (6): 107-111.doi: 10.16035/j.issn.1001-7283.2016.06.018

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The Effects of PEG Pretreatment on the Isozymes and Expression of Antioxidant Enzymes in Rice Leaves under Water Stress

Li Xuemei,Liu Chang,Liu Qianwen,Zhang Yudi,Li Xuemei   

  1. College of Life Science,Shenyang Normal University,Shenyang 110034,Liaoning,China
  • Received:2016-07-04 Revised:2016-08-24 Online:2016-12-15 Published:2018-08-26

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Abstract:

In this paper, polyethylene glycol (PEG6000) was used to simulate water stress, and changes of superoxide dismutase (SOD), peroxidase (POD), isoenzyme of catalase (CAT) and gene expression in rice leaves were studied in 10% PEG pretreatment, rewater and stronger water stress (15% PEG-6000). The results showed that PEG pretreatment improved the production of SOD, CAT and POD isozymes in leaves, and induced new isozymes. When subjected to a stronger stress, the activity of SOD isoenzyme bands II and IV increased, and new isoenzyme band III was observed; POD isoenzyme band II increased while band V decreased, and band VI of new POD isoenzyme was induced; Different isoenzymes of CAT showed different changes. In addition, Cu/Zn SOD, APX1 and CAT1 gene of direct 15% PEG-6000 stress (control group 2) at the transcriptional level were significantly up-regulated, whereas pretreatment complex water group and pretreatment CAT1 group compared with the control group 1 decreased slightly, pretreatment group APX1 similar to the control group 1. This showed that the 15% PEG-6000 made rice be hurt, but pretreatment of rice could improve resistance to anti oxidase gene and the control group 1 had a similar expression, indicating that when encountered strong water stress, the plants could resist water stress by regulating gene expression.

Key words: Rice, Water stress, Isozyme, Gene expression

Table 1

PCR amplified upstream and downstream primers nucleic acid sequences"

基因Gene 上游引物5′-3′Upstream primer 5′-3′ 下游引物5′-3′Downstream primer 5′-3′
Action GAAGATCACTGCCTTGCTCC CGATAACAGCTCCTCTTGGC
Cu/Zn-SOD AATGGTGAAGGCTGTTGCTGT TCTAACCCTGGAGTCCGATGA
APX1 AGGAAGGGAGGACAAACC TATTACAGAGGCGACTCACA
CAT1 GCCGTAGTCGTGAATAAAAT CTGTATCAGTCTTATTATCTCTTTC

Fig.1

The effect of PEG pretreatment on SOD isozymes of rice leaves under water stress1-Control group 1; 2-Pretreatment complex water group; 3-Control group 2; 4-Pretreatment group;the same below"

Fig.2

The effect of PEG pretreatment on POD isozymes of rice leaves under water stress"

Fig.3

The effect of PEG pretreatment on CAT isozymes of rice leaves under water stress"

Fig.4

The effect of PEG pretreatment on antioxidant enzyme gene expression of rice leaves under water stress"

[1] 雷东阳 . 水稻PFP基因的克隆及其遗传转化. 湖南农业大学学报, 2016,42(2):113-117.
doi: 10.13331/j.cnki.jhau.2016.02.001
[2] 王小萍, 王云, 唐晓波 , 等. 茶树抗旱机理和抗旱育种研究进展. 中国农学通报, 2016,32(13):12-17.
[3] 施溯筠, 陈翠云 . 外源一氧化氮供体SNP对UV-B辐射下红芸豆叶片中SOD、CAT和POD同工酶的影响. 兰州大学学报, 2009,45(4):78-82.
[4] 何兵, 王亚男, 李安奇 , 等. 土荆芥组培根分泌物对大豆根尖细胞的氧化损伤. 四川师范大学学报, 2013,36(3):440-444.
doi: 10.3969/j.issn.1001-8395.2013.03.028
[5] 孙彩霞, 刘志刚, 荆艳东 . 水分胁迫对玉米叶片关键防御酶系活性及其同工酶的影响. 玉米科学, 2003,11(1):63-66.
doi: 10.3969/j.issn.1005-0906.2003.01.021
[6] 周瑞莲, 杨淑琴, 黄清荣 , 等. 小叶锦鸡儿抗沙埋生长与抗氧化酶及同工酶变化的关系. 生态学报, 2015,35(9):3014-3022.
doi: 10.5846/stxb201306131701
[7] Bian S M, Jiang Y W . Reactive oxygen species,antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Scientia Horticulturae, 2009,120(10):264-270.
doi: 10.1016/j.scienta.2008.10.014
[8] 郭秀林, 刘子会, 赵会嶶 , 等. 热锻炼和高温胁迫下冬小麦热激蛋白与抗氧化酶基因表达. 北农学报, 2014,29(4):13-18.
doi: 10.7668/hbnxb.2014.04.003
[9] 张诗婉, 梅映学, 魏玮 , 等. PEG对Cd胁迫下水稻幼苗抗氧化酶活性的影响. 沈阳师范大学学报, 2015,33(3):437-442.
[10] 赵璞, 李梦, 及增发 , 等. 植物干旱响应生理对策研究进展. 中国农学通报, 2016,32(15):86-92.
[11] Bruce T J A, Matthes M C, Napier J A , et al. Stressful “memories” of plants:Evidence and possible mechanisms. Plant Science, 2007,173:603-608.
doi: 10.1016/j.plantsci.2007.09.002
[12] 邹琦 . 植物生理学试验指导.北京: 中国农业出版社, 2001.
[13] 胡能书 . 同工酶技术及其应用.湖南: 科学技术出版社, 1985.
[14] 孙德权, 郭启高, 胡玉林 , 等. 改良Trizol法提取香蕉叶片总RNA.广东农业科学, 2009(5):162-164.
[15] 刘世鹏, 曹娟云, 陈国梁 , 等. 干旱对组培枣苗过氧化物酶活性及其同工酶酶谱的影响. 安徽农业科学, 2007,35(5):1269-1271.
[16] 董守坤, 李雪凝, 赵坤 , 等. 干旱胁迫对春大豆根系保护酶活性的影响.作物杂志, 2015(2):163-165.
[17] Toyomasu T, Zennyoxi A . On the application of isoenzyme electrophoresis to identification of strains in Leurinus edodes. Mushroom Science, 1981,11:675-684.
[18] 王素平, 孙艳军 . H2O2预处理对低温下黄瓜幼苗抗氧化酶同工酶的影响. 江苏农业科学, 2013,41(6):123-125.
[19] 陈斌, 王亚男, 马丹炜 , 等. 土荆芥化感胁迫对玉米幼根抗氧化酶活性和基因表达的影响. 生态环境学报, 2015,24(10):1640-1646.
doi: 10.16258/j.cnki.1674-5906.2015.10.008
[20] 张秀海, 黄丛林, 沈元月 , 等. 植物抗旱基因研究进展.生物技术通报, 2001(4):21.
doi: 10.3969/j.issn.1002-5464.2001.04.005
[21] 许力, 李姣, 宋文强 , 等. 水杨酸通过提高抗氧化酶活性及基因表达缓解番茄低钾胁迫. 华北农学报, 2016,31(1):96-101.
doi: 10.7668/hbnxb.2016.01.016
[22] 李钰, 郑文寅, 冯春 , 等. 非生物逆境胁迫下普通小麦烟农19幼苗FeSOD基因表达分析.作物杂志, 2016(4):75-79.
doi: 10.16035/j.issn.1001-7283.2016.04.012
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