Crops ›› 2016, Vol. 32 ›› Issue (6): 107-111.doi: 10.16035/j.issn.1001-7283.2016.06.018

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The Effects of PEG Pretreatment on the Isozymes and Expression of Antioxidant Enzymes in Rice Leaves under Water Stress

Li Xuemei,Liu Chang,Liu Qianwen,Zhang Yudi,Li Xuemei   

  1. College of Life Science,Shenyang Normal University,Shenyang 110034,Liaoning,China
  • Received:2016-07-04 Revised:2016-08-24 Online:2016-12-15 Published:2018-08-26

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Abstract:

In this paper, polyethylene glycol (PEG6000) was used to simulate water stress, and changes of superoxide dismutase (SOD), peroxidase (POD), isoenzyme of catalase (CAT) and gene expression in rice leaves were studied in 10% PEG pretreatment, rewater and stronger water stress (15% PEG-6000). The results showed that PEG pretreatment improved the production of SOD, CAT and POD isozymes in leaves, and induced new isozymes. When subjected to a stronger stress, the activity of SOD isoenzyme bands II and IV increased, and new isoenzyme band III was observed; POD isoenzyme band II increased while band V decreased, and band VI of new POD isoenzyme was induced; Different isoenzymes of CAT showed different changes. In addition, Cu/Zn SOD, APX1 and CAT1 gene of direct 15% PEG-6000 stress (control group 2) at the transcriptional level were significantly up-regulated, whereas pretreatment complex water group and pretreatment CAT1 group compared with the control group 1 decreased slightly, pretreatment group APX1 similar to the control group 1. This showed that the 15% PEG-6000 made rice be hurt, but pretreatment of rice could improve resistance to anti oxidase gene and the control group 1 had a similar expression, indicating that when encountered strong water stress, the plants could resist water stress by regulating gene expression.

Key words: Rice, Water stress, Isozyme, Gene expression

Table 1

PCR amplified upstream and downstream primers nucleic acid sequences"

基因Gene 上游引物5′-3′Upstream primer 5′-3′ 下游引物5′-3′Downstream primer 5′-3′
Action GAAGATCACTGCCTTGCTCC CGATAACAGCTCCTCTTGGC
Cu/Zn-SOD AATGGTGAAGGCTGTTGCTGT TCTAACCCTGGAGTCCGATGA
APX1 AGGAAGGGAGGACAAACC TATTACAGAGGCGACTCACA
CAT1 GCCGTAGTCGTGAATAAAAT CTGTATCAGTCTTATTATCTCTTTC

Fig.1

The effect of PEG pretreatment on SOD isozymes of rice leaves under water stress1-Control group 1; 2-Pretreatment complex water group; 3-Control group 2; 4-Pretreatment group;the same below"

Fig.2

The effect of PEG pretreatment on POD isozymes of rice leaves under water stress"

Fig.3

The effect of PEG pretreatment on CAT isozymes of rice leaves under water stress"

Fig.4

The effect of PEG pretreatment on antioxidant enzyme gene expression of rice leaves under water stress"

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