Crops ›› 2018, Vol. 34 ›› Issue (1): 160-165.doi: 10.16035/j.issn.1001-7283.2018.01.026

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Effects of Starane on the Community Diversity of Maize Root Endophytes Analyzed Using High-Throughput Sequencing Technology

Wang Chunlei1,Fang Zhijun2,Xu Yanrui1,Lu Xiaoping1,Mu Chunhua2,Shan Kai1,Hao Lujiang1   

  1. 1 Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shangdong, China
    2 Maize Research Institute, Shandong Academic of Agricultural Sciences, Jinan 250100, Shangdong, China
  • Received:2017-09-07 Revised:2017-12-13 Online:2018-02-20 Published:2018-08-24

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Abstract:

The Illumina Miseq high-throughput sequencing technology was used to determine the V4-V5 variable region sequence of 16S rRNA and the effect of growing weed or weeding control with starane on the composition of the community structure of maize root endophytes in different periods. The research received a total of 166 647 effective sequences, 145 298 high-quality sequences, 4 464 OTUs from 5 maize root samples. The results of the analysis of Alpha diversity showed that the community diversity of maize root endophytes increased with the growth of maize plants. By weeding control with starane, the richness and evenness of the root community were decreased slightly at florescence stage, and obviously at maturation stage. Community composition analysis showed that by weeding control with starane, the distribution of bacteria in phylum and genus level changed greatly at florescence and maturation stages compared with the grass groups. The analysis of the gene function of endophytes showed that by weeding control with starane, the abundance of xenobiotics biodegradation metabolism was higher than the group of growing weed at florescence stage, and the abundance of carbohydrate metabolism increased at maturation stage. In brief, the result indicated that the spraying starane reduced the diversity of bacteria in maize roots, and the proportion of endophytic bacteria changed. The increase in the proportion of Pseudomonas was relevant with the xenobiotics biodegradation metabolism at florescence stage, and the increase in proportion of Enterobacter and Bacillus was relevant with the increase of carbohydrate metabolism.

Key words: High-throughput sequencing, Starane, Maize, Roots endophytes, Bacterial diversity

Table 1

Maize roots samples information"

编号
Number		 使它隆处理
Starane treatment		 采集时期
Collection stage		 采集日期
Collection date
LR81CK - 苗期Seedling 2016-06-24
LR82 + 花期Florescence 2016-08-24
LR82CK - 花期Florescence 2016-08-24
LR83 + 成熟期Maturation 2016-09-17
LR83CK - 成熟期Maturation 2016-09-17

Table 2

Sequence data statistics of samples"

样品
Sample		 有效序列数
Effective
sequence number		 高质量序列
High quality sequence		 比例(%)
Ratio		 OTU数
OTUs
LR81CK 28 916 27 226 94.16 485
LR82 36 203 31 590 87.26 924
LR82CK 34 414 27 056 78.62 958
LR83 32 665 29 873 91.45 805
LR83CK 34 449 29 553 85.79 1 292
合计Total 166 647 145 298 87.19 4 464

Fig.1

High quality sequences length distribution"

Table 3

Biological diversity indexes of roots endophytes"

样品Sample Chao1 ACE Simpson Shannon
LR81CK 279.00 379.60 0.60 2.34
LR82 711.00 852.91 0.96 6.07
LR82CK 736.00 839.58 0.96 6.59
LR83 620.00 714.06 0.93 5.50
LR83CK 1 120.00 1 194.33 0.96 6.77

Table 4

Sample flora distribution in phylum level %"

门Phylum LR81CK LR82 LR82CK LR83 LR83CK
变形菌门Proteobacteria 30.022 48.047 40.834 51.995 50.362
厚壁菌门Firmicutes 3.133 25.657 29.960 29.889 17.223
蓝藻菌门Cyanobacteria 60.596 10.908 12.934 5.843 9.915
放线菌门Actinobacteria 5.030 7.649 6.444 5.046 7.445
拟杆菌门Bacteroidetes 0.570 5.365 7.321 4.392 2.861
酸杆菌门Acidobacteria 0.328 1.694 1.275 1.894 5.546
绿弯菌门Chloroflexi 0.119 0.065 0.126 0.133 1.809
芽单胞菌门Gemmatimonadetes 0.041 0.026 0.008 0.102 1.559
浮霉菌门Planctomycetes 0.019 0.132 0.126 0.164 0.966
硝化螺旋菌门Nitrospirae 0.034 0.006 0.008 0.034 0.825
柔膜菌门Tenericutes 0.067 0.239 0.333 0.102 0.116
疣微菌门Verrucomicrobia 0.019 0.052 0.463 0.048 0.119
泉古菌门Crenarchaeota 0.007 0.013 0.015 0.055 0.470
装甲菌门Armatimonadetes 0.000 0.000 0.000 0.102 0.260
梭杆菌门Fusobacteria 0.000 0.023 0.031 0.024 0.029
广古菌门Euryarchaeota 0.000 0.013 0.008 0.014 0.022
迷踪菌门Elusimicrobia 0.000 0.000 0.000 0.000 0.033
螺旋体门Spirochaetes 0.000 0.000 0.011 0.000 0.000
互养菌门Synergistetes 0.000 0.000 0.011 0.000 0.000
绿菌门Chlorobi 0.000 0.000 0.000 0.000 0.011

Table 5

Sample flora distribution in genus level %"

属Genus LR81CK LR82 LR82CK LR83 LR83CK
链球菌属Streptococcus 6.835 17.833 14.959 23.453 16.950
肠杆菌属Enterobacter 40.055 6.388 15.440 21.940 4.577
莫拉氏菌属Moraxella 4.062 10.714 1.402 5.535 17.210
丛毛单胞菌属Comamonas 3.620 6.897 5.556 5.044 5.525
根瘤菌属Rhizobium 0.059 5.943 5.248 4.353 14.549
拟诺卡菌属Nocardiopsis 23.537 5.726 4.835 0.258 0.141
假单胞菌属Pseudomonas 1.505 4.390 3.334 4.751 4.144
黄单胞菌属Xanthomonas 3.194 6.593 4.105 2.145 2.662
芽孢杆菌属Bacillus 1.586 2.879 2.236 4.353 2.765
曼嗜甲壳菌属Chitinophaga 1.867 4.161 2.619 3.550 2.702
诺卡氏菌属Nocardia 1.327 2.113 1.933 3.132 2.411
慢生根瘤菌属Bradyrhizobium 0.825 1.664 0.810 1.888 2.089
动性球菌属Planococcus 3.436 1.637 2.231 0.402 0.338
草酸杆菌属Oxalobacter 1.084 4.563 4.601 1.517 1.385
鞘脂单胞菌属Sphingomonas 0.200 1.490 1.739 0.820 1.198
伯克氏菌属Burkholderia 0.944 1.136 2.033 1.899 1.000
毛螺菌属Lachnospira 0.987 1.704 4.706 0.452 0.630
布鲁氏菌属Brucella 0.583 0.927 0.810 1.006 1.271
生丝微菌属Hyphomicrobium 0.081 0.659 0.850 1.311 1.426
其他Others (128) 4.213(15) 12.584(48) 20.553(47) 12.192(53) 17.027(102)

Fig.2

The second level distribution of KEGG predicted by PICRUSt"

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