Crops ›› 2018, Vol. 34 ›› Issue (3): 123-128.doi: 10.16035/j.issn.1001-7283.2018.03.019

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and Antioxidant Activity of Tobacco Seedlings under Salt Stress

Zhang Kailun1,Chen Shouming2,Yin Hong3,Li Bin4,Xie Liangwen5,He Fan1   

  1. 1 College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2 Panzhihua Company, Sichuan Tobacco Company, Panzhihua 617000, Sichuan, China
    3 Liangshan Company, Sichuan Tobacco Company, Liangshan 615000, Sichuan, China
    4 Science and Technology Department, Sichuan Tobacco Corporation, China Tobacco Corporation, Chengdu 610041, Sichuan, China
    5 Tobacco Production and Management Department, Sichuan Tobacco Corporation, China Tobacco Corporation, Chengdu 610041, Sichuan, China
  • Received:2018-01-15 Revised:2018-03-27 Online:2018-06-20 Published:2018-06-20

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Abstract:

In order to explore the effect of exogenous nitric oxide (NO) on tobacco seedlings under salt stress and provide some references to understand the mechanism of salt resistance in tobacco production, tobacco variety K326 was used as research object and sodium nitroprusside (SNP) exogenous nitric oxide donor was used to study the physiological and antioxidant activities of tobacco seedlings under different treatments. The results showed that salt stress could significantly inhibit the normal growth and development of seedlings. After pretreatment with different concentrations of SNP, the leaf water content of tobacco seedlings increased significantly and the root activity increased, which increased the chlorophyll content and osmotic substance content. The antioxidant enzyme content in seedlings significantly increased, but hydrogen peroxide content and MDA content decreased, reducing the degree of membrane lipid peroxidation, protecting the cell structure against destroying, reducing leaf cell permeability. The results showed that exogenous nitric oxide could effectively improve the salt tolerance of tobacco seedlings, and had a significant concentration effect. Pretreatment using 0.10mmol/L SNP was the best for improving the salt tolerance of tobacco.

Key words: Nitric oxide, Tobacco, Salt stress, Physiology and biochemistry

Fig.1

Effects of exogenous NO on leaf water content, chlorophyll content and root vitality of tobacco seedlings under salt stress"

Fig.2

Effects of exogenous NO on contents of soluble sugar, proline and soluble protein in leaves of tobacco seedlings under salt stress"

Fig.3

Effects of exogenous NO on relative conductivity, hydrogen peroxide and malondialdehyde content in leaves of tobacco seedling under salt stress"

Fig.4

Effects of exogenous NO on the activities of SOD, POD, CAT and APX in tobacco seedlings under salt stress Effects of Exogenous Nitric Oxide on Physiology"

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