Crops ›› 2018, Vol. 34 ›› Issue (5): 121-126.doi: 10.16035/j.issn.1001-7283.2018.05.019

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Regulation of Exogenous 5-Aminolevulinic Acid on Photosynthesis and D1 Protein of Wheat Seedlings under Drought Stress

Wang Jianan,Li Xiaoyan,Wei Shimei,Zhao Huijie,Zhao Mingqi,Wang Yuexia   

  1. College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-05-04 Revised:2018-08-31 Online:2018-10-15 Published:2018-10-12
  • Contact: Yuexia Wang

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Abstract:

The objectives of this study were to elucidate the alleviation mechanism of exogenous 5-aminolevulinic acid (5-ALA) on the damage of wheat seedlings exposed to drought stress. The Aikang 58 wheat seedlings with two fully expanded leaves were used as the experimental materials. Three days after foliar application of 100mg/L 5-ALA, 20% polyethylene glycol 6000 (PEG-6000) was treated on the root to simulate the drought stress, followed by the photosynthetic physiological and biochemical characteristics and psbA gene expression analysis. The result showed that drought stress significantly decreased the relative water content and the chlorophyll content in wheat leaves. The pre-treatment of 5-ALA could obviously alleviate the injury caused by drought stress, inhibiting the decrease of net photosynthetic rate (Pn), actual photochemical efficiency (ΦPSⅡ), primary photochemical efficiency (Fv/Fm) and photochemical quenching coefficient (qP) values, as well as retarding the increase of non-photochemical quenching coefficient (qN) value. Meanwhile, the transcription of psbA gene and D1 protein content under drought stress were also improved by ALA pre-treatment. The present results proposed that the exogenous application of 5-ALA played a positive regulatory role in the photosynthetic rate and improved the expression level of psbA gene of wheat seedlings under the drought stress, thus accelerated the turnover of the damaged D1 protein and inhibits the degradation of chlorophyll in wheat leaves, which was the benefit of promoting wheat photosynthesis, thereby alleviating the damages caused by drought stress damages on wheat seedling.

Key words: Wheat, Photosynthesis, 5-aminolevulinic acid, psbA gene, D1 protein, PSⅡ, Chlorophyll fluorescence parameters

Fig.1

Relative water content of wheat seedlings under different treatmentsThe error bar is the standard error of 3 repetitions, different letters on the top of bars represent significant difference at P=0.05 level"

Fig.2

Effects of different treatments on chlorophyll content of wheat seedlings"

Fig.3

Effects of different treatments on the PSⅡ function of wheat seedlings"

Fig.4

Effects of different treatments on net photosynthetic rate of wheat seedlings"

Fig.5

Relative expression of psbA gene in wheat seedlings under different treatments"

Fig.6

D1 protein expression in leaves of wheat seedlings under different treatmentsA: SDS-PAGE electrophoretogram; B: Western blot"

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