Crops ›› 2022, Vol. 38 ›› Issue (4): 236-241.doi: 10.16035/j.issn.1001-7283.2022.04.033

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Effects of H2O2 on the Seed Germination of Peanut under Imbibition and Chilling Injury

Cui Yaʼnan(), Zhang Man, Zhang Penglei, Liu Bing, Hao Xi, Zang Xiuwang()   

  1. Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2021-10-27 Revised:2022-02-10 Online:2022-08-15 Published:2022-08-22
  • Contact: Zang Xiuwang E-mail:2369172533@qq.com;18638697801@163.com

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Abstract:

To study the effects of soaking seeds with H2O2 on peanut seed germination and physiological metabolism under imbibition and chilling injury, peanut varieties, Yuhua 9326 and Yuhua 37, were treated as materials soaking with 50mmol/L H2O2 and sterilized water as a low temperature control (5℃). The results showed that the germination vigor, germination rate and germination index of Yuhua 9326 treated with H2O2 increased by 31.25%, 18.52%, 50.53%, Yuhua 37 increased by 14.58%, 3.64%, 36.13%, respectively, seed electrical conductivities were significantly reduced by 47.67% and 23.67%, respectively, and the growth rate of germination indicators of Yuhua 9326 was higher than that of Yuhua 37. Compared with the control, the malondialdehyde content of Yuhua 9326 was decreased by 0.22%, 7.05% and 32.55% on the 0th, 4th and 6th day of germination, respectively, the soluble sugar contents were significantly reduced by 48.27%, 53.42% and 52.29%, respectively, the soluble protein contents were significantly increased by 59.66%, 13.04% and 43.76%, respectively, the abscisic acid (ABA) content were decreased and then increased, and the gibberellin content (GA) and the GA/ABA ratio showed a significant increasing trend. H2O2 seed soaking could improve chilling tolerance during the germination phase by maintaining the integrity of cell membranes, increasing soluble matter levels and regulating endogenous hormone levels, further increasing chilling resistance of seed under imbibition and chilling injury, so as to improve germination rate, germination vigor and germination index. This study provided a method and theoretical basis for the research and development of techniques to regulate chilling tolerance during the germination period of peanuts.

Key words: Peanut, Seed soaking with H2O2, Chilling imbibition, Germination, Chilling injury

Fig.1

Effects of H2O2 soaking treatments on electrical conductivity of peanut seed Different capital and lowercase letters indicate significant differences between treatments at the 0.01 and 0.05 level, respectively, the same below"

Table 1

Effects of H2O2 soaking treatments on percentage of seed radicle breaking through the testa %"

品种
Variety		 处理
Treatment		 露白率Percentage of seed radicle breaking through the testa
2d 3d 4d 5d 6d
豫花9326
Yuhua9326		 CK 31.82±4.55d 75.76±5.25c 87.88±2.62b 89.39±2.62ab 89.39±2.62ab
A1 80.30±9.46bc 96.97±5.25a 96.97±5.25a 96.97±5.25a 96.97±5.25a
豫花37
Yuhua37		 CK 19.05±4.76c 66.67±9.53b 88.89±5.49a 90.48±4.76a 92.06±5.50a
A1 53.97±16.72b 85.71±8.25a 88.89±5.50a 90.48±8.25a 92.06±7.27a

Table 2

Effects of H2O2 soaking treatments on the germination characteristics of peanut seed"

品种Variety 处理Treatment 发芽势Germination vigor (%) 发芽率Germination rate (%) 发芽指数Germination index
豫花9326
Yuhua 9326		 CK 72.73±0.00Bb 81.82±7.87Ab 2.95±0.15Bb
A1 95.45±7.87Aa 96.97±5.25Aa 4.45±0.42Aa
豫花37
Yuhua 37		 CK 76.19±14.29Aa 87.30±7.27Aa 2.84±0.41Ab
A1 87.30±7.28Aa 90.48±8.25Aa 3.87±0.35Aa

Fig.2

Effects of H2O2 soaking treatments on MDA content of peanut seed"

Fig.3

Effects of H2O2 soaking treatments on osmotic regulating substances content of peanut seed"

Fig.4

Effects of H2O2 soaking treatments on endogenous hormones content of peanut seed"

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