萌发期耐莠去津谷子种质资源筛选及评价

doi:10.16035/j.issn.1001-7283.2021.05.030

Abstract

Abstract:

The resistance of 100 foxtail millet varieties to atrazine during germination was studied, and the changes of germination potential, germination rate, bud length and root length under atrazine stress were analyzed. The results showed that the optimal concentration of atrazine was 2.0mL/L during the germination of foxtail millet. The variation coefficients of the relative values of 100 foxtail millet material in the germination period were as follows, germination relative to atrazine stress rate > relative germination potential > relative activity index > relative germination index > relative bud length > relative germination rate > relative root length under 2.0mL/L atrazine condition. According to the cluster analysis based on T value, the tested varieties were divided into four atrazine-tolerance classes, 24 high-tolerance varieties, 28 medium-tolerance varieties, 31 medium-sensitivity varieties and 17 high-sensitivity varieties. Fenxuan 3, Zigenguzi, Gong’ai 2, Shanxiwugu, Hebeixianggu and Caoguzi were the resources with strong atrazine-tolerance.

Key words: Foxtail millet, Atrazine tolerance, Germination stage, Comprehensive evaluation

Wang Yuting, Miao Xingfen, Wang Di. Screening and Evaluation of Atrazine-Resistant Germplasm Resources of Foxtail Millet at Germination Stage[J].Crops, 2021, 37(5): 194-204.

Figures/Tables 10

Table 1

Table 2

Table 3

Table 4

Trait relative values of 100 materials of foxtail millet under 2.0mL/L atrazine stress at germination stage %"

编号 Number	材料 Material		相对发芽势 Relative germination potential		相对发芽率 Relative germination rate		相对发芽指数 Relative germination index		相对活力指数 Relative activity index		相对根长 Relative root length		相对芽长 Relative bud length		发芽相对莠去津胁迫率 Germination relative to atrazine stress rate
1	嫩选15号		47.06		76.09		57.57		54.16		74.13		94.08		23.91
2	黄沙谷		70.63		84.25		77.83		68.32		84.23		87.42		15.75
3	赤谷10		77.17		88.97		82.83		60.86		83.28		87.00		11.03
4	龙谷33		75.00		89.60		84.95		63.22		83.28		52.10		10.40
5	超小粒51		70.63		85.61		76.53		61.71		80.23		90.17		14.39
6	千斤红		68.86		90.41		77.83		68.32		83.38		88.04		9.59
7	龙谷25		56.52		76.09		67.35		35.05		60.94		77.69		23.91
8	宇谷1号		73.45		86.40		83.40		69.72		96.35		98.32		13.60
9	龙谷34		76.98		79.43		82.86		72.48		83.87		79.78		20.57
10	钻头白		56.25		78.01		70.25		51.20		74.28		72.93		21.99
11	红粘谷		79.85		87.77		84.46		71.88		62.28		87.72		12.23
12	晋谷26		98.25		96.90		88.46		82.55		86.28		93.49		3.10
13	汾选3号		177.92		95.77		126.13		114.72		85.04		90.53		4.23
编号 Number	材料 Material		相对发芽势 Relative germination potential		相对发芽率 Relative germination rate		相对发芽指数 Relative germination index		相对活力指数 Relative activity index		相对根长 Relative root length		相对芽长 Relative bud length		发芽相对莠去津胁迫率 Germination relative to atrazine stress rate
14	六一谷		77.36		86.36		74.09		64.13		76.65		73.47		13.64
15	金穗黄金谷		65.69		76.98		72.93		52.40		73.18		72.41		23.02
16	龙谷31		67.86		62.12		67.19		49.23		74.18		72.91		37.88
17	红谷		78.45		85.92		85.82		77.81		80.25		74.46		14.08
18	神谷红谷		77.94		88.65		80.87		66.38		84.71		80.78		11.35
19	龙谷15		78.74		90.71		84.61		76.11		84.34		87.11		9.29
21	晋谷35		81.01		83.76		77.45		60.16		84.74		83.47		16.24
22	晋谷29		78.95		90.14		91.08		79.03		74.60		87.60		9.86
23	龙谷35		77.00		86.43		83.71		73.04		84.25		85.62		13.57
24	龙谷27		72.73		91.85		80.90		70.58		87.15		90.25		8.15
25	嫩选16		78.20		87.14		77.80		54.41		88.13		82.11		12.86
26	黄毛谷		79.86		92.36		97.60		76.61		85.41		89.96		7.64
27	嫩红谷		79.70		90.65		81.39		68.75		82.78		88.87		9.35
28	龙蛇谷		80.47		92.25		87.83		78.12		75.76		86.61		7.75
29	晋谷25		74.63		84.29		69.90		62.89		85.08		87.26		15.71
30	燕谷16		78.20		93.01		86.42		76.41		79.09		87.32		6.99
31	九谷20		79.09		90.34		88.19		67.97		82.57		78.48		9.66
32	红久谷		77.88		85.19		78.11		59.88		84.09		84.23		14.81
33	紫根谷子		100.00		95.77		95.06		86.49		86.80		90.99		4.23
34	金谷2号		75.74		93.10		80.44		65.20		87.14		89.00		6.90
35	糟谷		72.92		92.36		83.10		71.23		85.72		89.97		7.64
36	小红谷		74.10		92.36		82.36		71.32		85.07		88.32		7.64
37	公矮5号		64.39		73.05		66.06		59.55		70.78		69.94		26.95
38	嫩选17		79.21		84.11		76.49		59.89		81.73		76.89		15.89
39	济谷16		74.14		80.77		67.88		53.52		83.94		81.05		19.23
40	红吨谷		68.87		81.67		66.22		57.14		84.36		85.75		18.33
41	小黄谷		75.54		92.36		87.49		77.86		85.82		86.73		7.64
42	大叶青粘谷		78.69		92.25		85.15		65.55		85.71		86.21		7.75
43	栗谷		77.69		89.78		85.18		68.14		84.51		89.02		10.22
44	冀谷25		71.74		85.25		78.60		68.85		81.44		76.66		14.75
45	冀谷11		78.95		85.82		72.17		53.01		85.15		79.98		14.18
46	大粒系		68.53		93.15		84.62		75.00		83.96		87.73		6.85
47	赤谷6号		74.79		92.42		84.17		66.70		86.21		90.27		7.58
48	铁变16		67.61		93.20		81.44		72.42		84.79		79.28		6.80
49	石火谷子		76.79		92.80		85.46		60.20		76.66		88.89		7.20
50	晋谷40		89.15		96.15		84.19		75.79		90.40		90.05		3.85
51	白苗白流沙		70.87		85.05		67.05		60.22		89.06		70.36		14.95
52	大白谷		83.33		96.18		85.27		78.78		85.79		92.24		3.82
53	嫩09-1		70.54		88.64		81.22		73.35		92.92		89.33		11.36
54	公矮2		93.80		97.84		93.29		84.43		86.61		90.55		2.16
55	赤谷4		79.38		89.00		75.43		67.46		81.18		90.16		11.00
56	九谷8		69.72		82.14		68.54		62.10		83.89		89.46		17.86
57	红谷2014		76.76		93.38		82.37		69.43		84.06		90.43		6.62
58	黄谷		73.76		87.86		79.72		69.67		78.30		84.36		12.14
59	当地种		80.29		91.11		83.42		65.68		81.58		78.63		8.89
60	临秋变		76.22		93.79		80.07		70.23		86.44		87.65		6.21
61	晋21		78.72		93.06		83.90		73.26		85.77		87.01		6.94
62	大青苗		75.68		93.88		84.40		76.76		85.43		90.99		6.12
63	公谷66		79.09		93.13		80.32		68.84		79.80		85.88		6.87
64	大糟谷		78.03		93.33		83.33		74.49		87.02		89.32		6.67
编号 Number		材料 Material		相对发芽势 Relative germination potential		相对发芽率 Relative germination rate		相对发芽指数 Relative germination index		相对活力指数 Relative activity index		相对根长 Relative root length		相对芽长 Relative bud length		发芽相对莠去津胁迫率 Germination relative to atrazine stress rate
65		龙谷30		76.92		92.86		78.12		60.44		84.62		77.53		7.14
66		小苗谷		72.46		92.41		85.59		74.61		83.62		87.25		7.59
67		四六钱		74.82		93.10		83.18		64.49		85.10		77.48		6.90
68		龙谷32		74.07		94.12		83.96		82.58		87.49		98.40		5.88
69		昭杂10号		75.24		85.98		76.60		53.13		82.40		68.99		14.02
70		PZ55dw2		79.13		94.03		91.02		70.50		88.77		77.51		5.97
71		龙谷35		75.96		83.70		75.87		65.94		77.37		86.37		16.30
72		张杂6		77.42		87.05		80.07		68.33		84.28		84.82		12.95
73		黄金吨谷		62.50		77.04		66.01		58.14		73.48		77.18		22.96
74		山东留山		91.20		95.56		84.91		73.96		85.04		87.13		4.44
75		晋育红谷		72.92		93.06		80.46		68.86		84.97		85.58		6.94
76		燕谷二号		69.00		73.83		77.28		67.27		74.34		73.49		26.17
77		小白谷二		78.03		91.11		78.45		68.52		85.43		87.67		8.89
78		昭杂11号		78.00		83.59		69.03		60.27		87.09		87.27		16.41
79		红谷鸟不弹		78.79		94.48		87.86		79.22		85.44		90.14		5.52
80		山西乌谷		93.39		95.83		88.67		80.55		94.73		90.90		4.17
81		赤谷8号		78.26		93.24		84.59		73.09		84.93		86.40		6.76
82		红谷1号		78.70		81.25		77.24		64.03		87.27		82.93		18.75
83		金谷1		60.20		80.56		66.88		59.68		90.08		89.36		19.44
84		河北香谷		81.29		95.86		85.96		82.14		94.79		95.33		4.14
85		红谷09-1		79.71		93.75		79.10		61.50		78.71		77.78		6.25
86		红钙谷		79.29		94.48		84.69		80.79		84.15		95.42		5.52
87		黑谷		79.26		93.01		81.16		72.17		82.38		88.91		6.99
88		金谷8		62.77		84.72		72.03		61.83		80.69		85.31		15.28
89		草谷子		80.00		95.92		80.76		73.73		86.56		91.32		4.08
90		刀把齐		79.17		81.44		86.07		70.66		84.84		74.85		18.56
91		小香谷		79.07		92.11		86.07		70.66		83.91		82.15		7.89
92		勾根		76.64		84.09		81.86		71.21		86.17		87.05		15.91
93		公矮8号		80.15		92.91		82.75		72.51		84.15		87.56		7.09
94		紫根白		69.30		77.44		74.52		73.12		74.38		78.39		22.56
95		龙谷20		79.10		93.06		82.52		72.75		83.92		87.43		6.94
96		白根红粘谷		67.67		76.06		68.41		54.64		67.11		78.78		23.94
97		红谷75		69.81		88.70		70.91		62.39		87.25		87.99		11.30
98		燕谷18		71.84		88.64		77.33		68.32		79.56		88.28		11.36
99		胜谷1号		68.80		83.21		65.75		51.98		85.29		78.70		16.79
100		嫩江16		71.15		84.35		67.77		59.87		84.04		88.43		15.65
最大值Max.				177.92		97.67		126.13		114.72		96.35		98.40		37.88
最小值Min.				47.06		62.12		57.57		36.19		60.94		52.10		2.33
均值Mean				76.69		83.33		80.43		68.32		82.71		84.73		11.67
标准差Standard deviation				12.78		6.46		8.75		10.11		5.99		7.16		6.45
变异系数 Coefficient of variation (%)				16.66		7.31		10.81		14.72		7.24		8.45		55.29

Table 4

Table 5

Fig.1

Table 6

Table 7

Table 8

Comprehensive evaluation of resistance to atrazine of foxtail millet during germination period"

编号Number		材料Material		u(1)		u(2)		u(3)		u(4)		u(5)		u(6)	T值T value			耐药性Herbicide resistance
1		嫩选15号		0.050		0.419		0.065		0.067		0.472		0.281	0.160			高感
2		黄沙谷		0.637		0.709		0.539		0.548		0.000		0.548	0.616			中耐
3		赤谷10		0.621		0.806		0.501		0.321		0.369		0.516	0.627			中耐
4		龙谷33		0.340		0.532		0.167		0.404		0.387		0.288	0.369			中感
5		超小粒51		0.369		0.548		0.385		0.563		0.236		0.491	0.421			中感
6		千斤红		0.657		0.855		0.552		0.696		0.458		0.868	0.690			中耐
7		龙谷25		0.185		0.419		0.078		0.122		0.491		0.295	0.237			高感
8		宇谷1号		0.305		0.467		0.308		0.500		0.440		0.442	0.361			中感
9		龙谷34		0.565		0.532		0.616		0.721		0.460		0.413	0.567			中感
10		钻头白		0.280		0.500		0.283		0.310		0.489		0.526	0.346			中感
11		红粘谷		0.554		0.693		0.616		0.690		0.101		0.512	0.587			中耐
12		晋谷26		0.486		0.742		0.680		0.657		1.000		0.838	0.604			中耐
13		汾选3号		1.000		0.919		1.000		1.000		0.886		0.808	0.975			高耐
14		六一谷		0.197		0.564		0.296		0.261		0.912		0.326	0.329			中感
15		金穗黄金谷		0.205		0.451		0.103		0.299		0.942		0.574	0.287			高感
16		龙谷31		0.133		0.048		0.219		0.207		0.626		0.477	0.147			高感
17		红谷		0.548		0.693		0.411		0.631		0.200		0.617	0.559			中感
18		神谷红谷		0.748		0.742		0.603		0.782		0.439		0.823	0.720			中耐
19		龙谷15		0.508		0.774		0.526		0.661		0.486		0.743	0.585			中耐
20		磨里谷		0.594		0.806		0.334		0.872		0.127		0.582	0.611			中耐
21		晋谷35		0.048		0.306		0.065		0.072		0.475		0.792	0.137			高感
22		晋谷29		0.740		0.790		0.590		0.928		0.336		0.846	0.730			高耐
23		龙谷35		0.508		0.677		0.231		0.622		0.511		0.825	0.524			中感
24		龙谷27		0.521		0.726		0.270		0.811		0.351		0.746	0.550			中感
25		嫩选16		0.409		0.693		0.578		0.000		0.402		0.767	0.484			中感
26		黄毛谷		0.817		0.871		0.667		0.783		0.400		0.822	0.796			高耐
27		嫩红谷		0.563		0.758		0.603		0.616		0.502		1.000	0.621			中耐
28		龙蛇谷		0.607		0.839		0.565		0.651		0.313		0.931	0.654			中耐
29		晋谷25		0.425		0.629		0.526		0.536		0.369		0.770	0.495			中感
30		燕谷16		0.726		0.871		0.578		0.818		0.349		1.000	0.737			高耐
31		九谷20		0.469		0.839		0.360		0.317		0.299		0.721	0.535			中感
32		红久谷		0.091		0.209		0.283		0.174		0.353		0.715	0.164			高感
33		紫根谷子		0.907		0.919		0.975		0.988		0.680		0.865	0.915			高耐
34		金谷2号		0.638		0.903		0.565		0.615		0.293		0.725	0.682			中耐
35		糟谷		0.731		0.871		0.590		0.718		0.225		0.729	0.730			高耐
36		小红谷		0.773		0.871		0.565		0.824		0.200		0.821	0.754			高耐
37		公矮5号		0.376		0.387		0.334		0.337		0.428		0.000	0.370			中感
38		嫩选17		0.000		0.177		0.270		0.193		0.473		0.457	0.108			高感
39		济谷16		0.168		0.419		0.347		0.228		0.267		0.660	0.264			高感
40		红吨谷		0.150		0.306		0.180		0.312		0.260		0.710	0.210			高感
41		小黄谷		0.742		0.871		0.590		0.740		0.443		0.795	0.745			高耐
42		大叶青粘谷		0.501		0.645		0.475		0.459		0.259		0.650	0.524			中感
43		栗谷		0.687		0.709		0.539		0.494		0.239		0.727	0.650			中耐
44		冀谷25		0.286		0.403		0.090		0.335		0.056		0.562	0.287			高感
45		冀谷11		0.416		0.677		0.590		0.509		0.446		0.491	0.509			中感
46		大粒系		0.699		0.919		0.501		0.713		0.361		0.561	0.716			中耐
47		赤谷6号		0.593		0.693		0.385		0.421		0.508		0.730	0.582			中耐
48		铁变16		0.736		0.935		0.475		0.822		0.385		0.459	0.741			高耐
49		石火谷子		0.413		0.596		0.347		0.234		0.449		0.820	0.447			中感
50		晋谷40		0.498		0.742		0.719		0.548		0.715		0.687	0.598			中耐
编号Number	材料Material		u(1)		u(2)		u(3)		u(4)		u(5)		u(6)			T值T value	耐药性Herbicide resistance
51	白苗白流沙		0.146		0.193		0.180		0.241		0.499		0.321			0.181	高感
52	大白谷		0.545		0.758		0.693		0.475		0.296		0.556			0.605	中耐
53	嫩09-1		0.436		0.613		0.257		0.547		0.521		0.522			0.466	中感
54	公矮2		0.823		0.919		0.796		0.396		0.472		0.318			0.806	高耐
55	赤谷4		0.097		0.161		0.231		0.351		0.373		0.735			0.158	高感
56	九谷8		0.204		0.209		0.219		0.308		0.800		0.786			0.239	高感
57	红谷2014		0.712		0.774		0.642		0.770		0.206		0.588			0.703	中耐
58	黄谷		0.640		0.709		0.578		0.519		0.138		0.782			0.627	中耐
59	当地种		0.774		0.709		0.654		0.720		0.121		0.682			0.717	中耐
60	临秋变		0.753		0.919		0.642		0.655		0.221		0.628			0.755	高耐
61	晋21		0.750		0.887		0.667		0.666		0.139		0.638			0.747	高耐
62	大青苗		0.908		0.952		0.680		0.712		0.140		0.594			0.851	高耐
63	公谷66		0.369		0.693		0.360		0.436		0.130		0.641			0.445	中感
64	大糟谷		0.755		0.758		0.565		0.595		0.189		0.561			0.703	中耐
65	龙谷30		0.574		0.822		0.526		0.507		0.427		0.575			0.621	中耐
66	小苗谷		0.752		0.887		0.526		0.743		0.411		0.724			0.744	高耐
67	四六钱		0.722		0.903		0.578		0.647		0.352		0.638			0.731	高耐
68	龙谷32		0.665		0.790		0.526		0.627		0.435		0.655			0.669	中耐
69	昭杂10号		0.108		0.209		0.257		0.277		0.578		0.634			0.182	高感
70	PZ55dw2		0.567		0.758		0.411		0.549		0.420		0.633			0.589	中耐
71	龙谷35		0.421		0.548		0.257		0.488		0.593		0.669			0.443	中感
72	张杂6		0.555		0.677		0.475		0.549		0.232		0.641			0.564	中感
73	黄金吨谷		0.299		0.403		0.334		0.544		0.580		0.402			0.352	中感
74	山东留山		0.655		0.806		0.706		0.603		0.726		0.635			0.699	中耐
75	晋育红谷		0.799		0.887		0.590		0.747		0.361		0.696			0.776	高耐
76	燕谷二号		0.128		0.000		0.129		0.221		0.519		0.702			0.121	高感
77	小白谷二		0.587		0.709		0.565		0.746		0.685		0.861			0.629	中耐
78	昭杂11号		0.235		0.451		0.244		0.274		0.739		0.503			0.312	中感
79	红谷鸟不弹		0.726		0.935		0.578		0.790		0.673		0.793			0.761	高耐
80	山西乌谷		0.747		0.952		0.693		0.876		0.623		0.870			0.794	高耐
81	赤谷8号		0.692		0.952		0.629		0.718		0.462		0.739			0.742	高耐
82	红谷1号		0.512		0.613		0.334		0.614		0.458		0.758			0.520	中感
83	金谷1		0.303		0.596		0.000		0.552		0.601		0.877			0.365	中感
84	河北香谷		0.836		0.968		0.693		0.978		0.775		0.905			0.856	高耐
85	红谷09-1		0.735		0.903		0.654		0.743		0.760		0.735			0.768	高耐
86	红钙谷		0.858		0.935		0.667		0.979		0.392		0.891			0.843	高耐
87	黑谷		0.748		0.871		0.616		0.790		0.508		0.777			0.756	高耐
88	金谷8		0.583		0.693		0.347		0.446		0.385		0.491			0.566	中感
89	草谷子		0.809		1.000		0.731		0.915		0.874		0.864			0.854	高耐
90	刀把齐		0.129		0.000		0.219		0.329		0.680		0.707			0.144	高感
91	小香谷		0.302		0.419		0.116		0.467		0.552		0.748			0.328	中感
92	勾根		0.650		0.516		0.590		0.678		0.334		0.725			0.601	中耐
93	公矮8号		0.685		0.839		0.590		0.770		0.541		0.802			0.711	中耐
94	紫根白		0.412		0.387		0.257		0.320		0.574		0.591			0.389	中感
95	龙谷20		0.665		0.887		0.603		0.681		0.492		0.708			0.708	中耐
96	白根红粘谷		0.504		0.467		0.398		0.341		0.019		0.485			0.457	中感
97	红谷75		0.289		0.370		0.193		0.596		0.785		0.960			0.336	中感
98	燕谷18		0.406		0.613		0.193		0.602		0.526		0.838			0.448	中感
99	胜谷1号		0.323		0.483		0.347		0.407		0.472		0.633			0.378	中感
100	嫩江16		0.139		0.290		0.193		0.406		0.613		0.833			0.220	高感
权重Weight			0.534		0.245		0.128		0.049		0.035		0.010

Table 8

Fig. 2

References 20

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材料 Material	发芽势Germination potential					发芽率Germination rate
材料 Material	0.0mL/L	0.5mL/L	1.0mL/L	2.0mL/L	3.0mL/L	0.0mL/L	0.5mL/L	1.0mL/L	2.0mL/L	3.0mL/L
嫩选15 Nenxuan 15	89.33aA	86.00aA	82.67aA	79.33aA	52.00bB	92.67aA	91.33aAB	88.00abAB	83.33bB	69.33cC
龙谷25 Longgu 25	76.67aA	68.00bB	60.67cC	54.67dD	40.67eE	92.00aA	88.00abA	85.33bA	74.67cB	59.33dC
公矮5号Gong’ai 5	81.33aA	78.00abA	70.67bA	54.00cB	44.00dB	90.67aA	82.67bB	77.33cB	68.00dC	50.00eD
均值Mean	82.44	77.33	71.34	62.67	45.56	91.78	87.33	83.55	75.33	59.55
变异系数 Coefficient of variation (%)	8.03	10.82	14.30	21.17	14.85	2.54	4.58	6.31	9.20	15.37

材料 Material	死亡天数Days of death
材料 Material	0.0mL/L	0.5mL/L	1.0mL/L	2.0mL/L	3.0mL/L
嫩选15 Nenxuan 15	0	0	0	11	10
龙谷25 Longgu 25	0	0	0	12	10
公矮5号Gong’ai 5	0	0	0	15	12
均值Mean	0	0	0	12.67	10.67
变异系数 Coefficient of variation (%)	0	0	0	14.21	9.37

性状 Trait	相对发芽势 Relative germination potential	相对发芽率 Relative germination rate	相对发芽指数 Relative germination index	相对活力指数 Relative activity index	相对根长 Relative root length	相对芽长 Relative bud length	发芽相对莠去津胁迫率 Germination relative to atrazine stress rate
相对发芽势Relative germination potential	1
相对发芽率Relative germination rate	0.48^**	1
相对发芽指数Relative germination index	0.78^**	0.69^**	1
相对活力指数Relative activity index	0.70^**	0.66^**	0.85^**	1
相对根长Relative root length	0.27^**	0.46^**	0.27^**	0.34^**	1
相对芽长Relative bud length	0.22^*	0.49^**	0.29^**	0.48^**	0.35^**	1
发芽相对莠去津胁迫率 Germination relative to atrazine stress rate	-0.48^**	-0.98^**	-0.69^**	-0.66^**	-0.46^**	-0.49^**	1

指标 Index	主成分1 Principal component 1	主成分2 Principal component 2
发芽势 Germination potential	0.88	0.00
发芽率 Germination rate	0.92	-0.12
发芽指数 Germination index	0.96	-0.14
活力指数 Vitality index	0.89	0.12
根长Root length	-0.10	0.87
芽长Bud length	0.48	0.60

Screening and Evaluation of Atrazine-Resistant Germplasm Resources of Foxtail Millet at Germination Stage

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主成分 Principal component	特征值 Eigen value	贡献率 Contribution rate (%)	累计贡献率 Accumulated contribution rate (%)
1	3.59	59.88	59.88
2	1.16	19.41	79.29
3	0.70	11.60	90.88
4	0.28	4.67	95.55
5	0.21	3.47	99.02
6	0.06	0.98	100.00

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