Crops ›› 2025, Vol. 41 ›› Issue (2): 79-85.doi: 10.16035/j.issn.1001-7283.2025.02.011

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Identification and Comprehensive Evaluation of Salt Tolerance in Different Growth Periods under Na2SO4 Stress in White Mustard

Fan Huiling1,2(), Zhao Xiaojiang1, Lu Yan1   

  1. 1College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, Gansu, China
    2College of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, China
  • Received:2023-12-01 Revised:2024-03-05 Online:2025-04-15 Published:2025-04-16

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Abstract:

In order to investigate germination, the morphological and physiological characteristics of Sinapis alba L. in response to Na2SO4 stress at different growth stages, and to screen out white mustard of strong salt tolerance, the filter paper bed germination method was adopted. Treatments were carried out with 0, 30, 60, 90, 120, 150, 180, 210 mmol/L of Na2SO4, and the germination rate, radical length, hypocotyl length, salt-tolerance critical value and salt-tolerance limit value were determined. In addition, the pot cultivation method was employed. The white mustard strains were stressed with 90, 120, 150 mmol/L of Na2SO4, and malondialdehyde, proline, photosynthetic pigment, water content and plant height were determined. Both principal component analysis and fuzzy mathematical membership function method were used for comprehensive evaluation. The results showed that 90 mmol/L Na2SO4 stress was suitable for screening white mustard germplasm, the threshold value of salt tolerance was 97-118 mmol/L, and the limit value of salt tolerance was 239-251 mmol/L. There were little correlation among the salt tolerance at the germination stage, seedling stage and flowering stage, the strongest salt tolerance was 11 baijie 3-1 line at germination stage and 12 baijie 15-7 line at flowering stage. The content of leaf proline, water content and chlorophyll a could be used as the reliable indexes to identify salt tolerance of white mustard.

Key words: White mustard (Sinapis alba), Na2SO4 stress, Physilogical index, Principal component analysis, Membership function method

Table 1

Effects of Na2SO4 on germination rate, hypocotyl length and radical length in three S.alba lines"

品系Line 盐浓度Salt concentration (mmol/L) 胚根长Radical length (cm) 胚轴长Hypocotyl length (cm) 发芽率Germination rate (%)
11白芥3-1
11 baijie 3-1		 0 1.78±0.30a 0.78±0.70a 86.72±9.41a
30 2.20±0.06a 3.36±0.81b 93.30±9.40a
60 1.36±0.41ab 2.84±0.61c 93.30±9.41a
90 0.52±0.20ab 2.40±0.80c 70.00±4.71b
120 0.17±0.10b 1.53±0.60d 36.67±4.70c
150 0.00±0.00b 0.00±0.00e 23.31±4.70c
180 0.00±0.00b 0.00±0.00e 6.67±0.03d
210 0.00±0.00b 0.00±0.00e 3.33±4.70d
12白芥15-7
12 baijie 15-7		 0 2.50±0.80a 3.80±0.51a 86.71±1.40b
30 2.40±0.20b 3.60±0.52a 100.00±0.00a
60 2.50±0.11a 3.60±0.80a 100.00±0.01a
90 1.90±0.52c 3.00±0.60ab 56.70±2.70c
120 0.20±0.10d 0.70±0.20c 33.30±2.30d
150 0.20±0.10d 0.00±0.00d 16.71±4.70e
180 0.00±0.00d 0.00±0.00d 0.00±0.00f
210 0.00±0.00d 0.00±0.00d 0.00±0.00f
13白芥15-2
13 baijie 15-2		 0 1.62±0.81b 3.50±0.50a 96.73±4.71a
30 2.44±0.20a 3.80±0.50a 90.00±4.70a
60 2.02±0.60b 3.74±0.70a 70.00±1.73b
90 0.38±0.20c 1.83±0.10c 50.00±2.71c
120 0.40±0.60c 3.50±1.10a 13.33±0.00e
150 0.30±0.00c 3.00±1.10ab 26.17±2.30d
180 0.20±0.20cd 2.00±0.10c 6.73±0.90f
210 0.00±0.00d 0.00±0.00d 0.00±0.00g

Table 2

Salt tolerance critical values and salt tolerance limit values of three S.alba lines under Na2SO4 stress"

品系
Line		 临界值
Critical value
(mmol/L)		 极限值
Limit value
(mmol/L)		 排序
Sequence
11白芥3-1 11 baijie 3-1 118 251 1
12白芥15-7 12 baijie 15-7 113 243 2
13白芥15-2 13 baijie 15-2 97 239 3

Fig.1

Changes of MDA content, water content, proline, and plant height under Na2SO4 stress in S.alba leaves Different lowercase letters indicate significant difference (P < 0.05)."

Table 3

Contents of chlorophyll a, b and carotenoid under Na2SO4 stress in S.alba leaves"

品系
Line		 盐浓度
Salt concentration (mmol/L)		 叶绿素a
Chlorophyll a (mg/g)		 叶绿素b
Chlorophyll b (mg/g)		 类胡萝卜素
Carotenoid (mg/g)
11白芥3-1
11 baijie 3-1		 0 14.53a 4.88a 3.17a
90 13.20b 4.78a 2.71b
120 12.54c 3.95b 2.63b
150 11.20d 3.02c 2.26c
12白芥15-7
12 baijie 15-7		 0 16.29a 6.31a 2.85a
90 13.58b 5.12b 2.56a
120 12.51c 4.36c 2.39a
150 10.95d 3.75d 2.04b
13白芥15-2
13 baijie 15-2		 0 16.84a 6.09a 2.59a
90 13.78b 6.05a 2.51a
120 12.14c 4.25b 2.49a
150 8.84d 3.11c 2.27b

Fig.2

Effects of Na2SO4 stress on growth and development of S.alba"

Table 4

Principal component analysis of traits"

指标
Index		 主成分Principal component
1 2
脯氨酸Proline -0.979 0.043
叶绿素a Chl a 0.971 0.141
叶绿素b Chl b 0.970 -0.939
相对含水量Relative water content 0.939 0.183
株高Plant height 0.898 -0.202
MDA 0.839 -0.433
类胡萝卜素Carotenoid 0.419 0.882
特征值Eigenvalue 5.411 1.071
累计贡献率
Cumulative contribution rate (%)		 77.302
 92.599

Table 5

Evaluation of tolerance to salt by membership function method"

指标
Index		 隶属值Membership function value
11白芥3-1 11 baijie 3-1 12白芥15-7 12 baijie 15-7 13白芥15-2 13 baijie 15-2
脯氨酸Proline 0.541 0.548 0.507
叶绿素b Chl b 0.641 0.559 0.553
叶绿素a Chl a 0.525 0.527 0.526
相对含水量Relative water content 0.516 0.458 0.541
株高Plant height 0.515 0.575 0.487
MDA 0.407 0.459 0.519
类胡萝卜素Carotenoid 0.344 0.450 0.466
隶属平均值Membership average 0.498 0.517 0.514
耐盐性排序Order of salt resistance 3 1 2
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