Crops ›› 2016, Vol. 32 ›› Issue (2): 21-26.doi: 10.16035/j.issn.1001-7283.2016.02.004

;

Previous Articles     Next Articles

Identification of Cold Tolerance at Germination Stage in Maize Inbred Lines

Zhang Xiaocong1,Zhou Yu1,Zhang Lin1,Di Hong1,Weng Jianfeng2,Li Xinhai2,Wang Zhenhua1   

  1. 1 College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
    2 Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Crop Molecular Breeding,Beijing 100081,China
  • Received:2016-01-27 Revised:2016-02-23 Online:2016-04-15 Published:2018-08-26
  • Contact: Xinhai Li,Zhenhua Wang

RichHTML

12

PDF (PC)

164

Abstract:

Cold stress is one of the major meteorological disasters in the spring maize-growing area in northern China. Cold tolerance of eight inbred lines widely used in northeastern China was evaluated through a laboratory germination experiment under low temperature stress. Germination potential, germination rate, average time to germination, germination index, dry weight of seedlings, and vigor index at normal temperature (25℃) and low temperature (10℃) were measured, respectively, and the ratios (relative values) of trait values at low temperature to those at normal temperature were used as indicators for cold tolerance. The cluster analysis revealed that the eight inbred lines could be classified into three groups: PH4CV, Ji 419, and Ji 495 belonged to high cold tolerance, H21 belonged to intermediate cold tolerance, and W9706, Huangzaosi, Danhuang 02, and Si 279 belonged to low cold tolerance. The correlation analysis showed that the relative values of germination rate, germination index, dry weight of seedlings, and vigor index were highly significantly correlated with each other, and they were suitable indicators for cold tolerance. This experiment provided guidance to identify and screen maize germplasm with cold tolerance at the germination stage on a large scale.

Key words: Maize, Germination, Cold tolerance, Cluster analysis, Correlation analysis

Table 1

Changes of germination potential and germination rate in maize inbred lines under low temperature stress %"

自交系
Inbred line		 发芽势Germination potential 发芽率Germination rate
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 98.89a 32.22ab 0.33 100.00a 96.67a 0.97
吉495 Ji 495 96.67a 26.67b 0.28 97.78a 95.56a 0.98
吉419 Ji 419 80.00c 33.33a 0.42 90.00c 86.67b 0.96
H21 95.56ab 28.89b 0.30 97.78a 72.22c 0.74
四279 Si 279 91.11b 36.67a 0.40 96.67ab 62.22d 0.64
丹黄02Danhuang 02 78.89c 12.22c 0.15 90.00c 61.11d 0.68
W9706 75.56c 13.33c 0.18 93.33bc 47.78e 0.51
黄早四Huangzaosi 76.67c 14.44c 0.19 94.44bc 56.67d 0.60

Table 2

Changes of average time to germination and germination index in maize inbred lines under low temperature stress"

自交系
Inbred line		 平均发芽时间（d）
Average time to germination		 发芽指数
Germination index
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 3.09c 18.05c 5.84 9.78ab 1.76a 0.18
吉495 Ji 495 3.37bc 20.15c 5.98 8.24cd 1.40ab 0.17
吉419 Ji 419 3.65ab 21.24bc 5.82 7.35de 1.25bc 0.17
H21 3.49b 21.22bc 6.08 10.47a 1.43ab 0.14
四279 Si 279 3.62ab 23.24b 6.42 8.93bc 1.16bc 0.13
丹黄02Danhuang 02 3.90a 26.71a 6.85 7.41de 0.89cd 0.12
W9706 3.81a 23.47ab 6.16 6.53e 0.74d 0.11
黄早四Huangzaosi 3.95a 23.98ab 6.07 7.37de 0.88cd 0.12

Table 3

Changes of dry weight of seedlings and vigor index in maize inbred lines under low temperature stress g"

自交系
Inbred line		 幼苗干重Dry weight of seedlings 活力指数Vigor index
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 0.12ab 0.05a 0.38 1.19a 0.08a 0.07
吉495 Ji 495 0.13a 0.04ab 0.32 1.11a 0.06ab 0.05
吉419 Ji 419 0.12ab 0.04ab 0.35 0.91ab 0.05ab 0.06
H21 0.10c 0.02c 0.22 1.09a 0.03b 0.03
四279 Si 279 0.08d 0.01cd 0.18 0.76bc 0.02b 0.02
丹黄02Danhuang 02 0.09cd 0.01cd 0.16 0.70bc 0.01b 0.02
W9706 0.09cd 0.02c 0.17 0.61c 0.01b 0.02
黄早四Huangzaosi 0.08d 0.02c 0.18 0.61c 0.01b 0.02

Fig.1

Cluster analysis of eight maize inbred lines"

Table 4

The mean relative values of traits in three groups of maize inbred lines with different cold tolerance"

类群
Group		 相对发芽势
Relative germination potential		 相对发芽率
Relative germination
rate		 相对平均发芽时间
Relative average time
to germination		 相对发芽指数
Relative germination
index		 相对幼苗干重
Relative dry weight
of seedlings		 相对活力指数
Relative vigor
index
I 0.34 0.97 5.88 0.17 0.35 0.06
II 0.30 0.74 6.08 0.14 0.22 0.03
III 0.23 0.61 6.38 0.12 0.17 0.02

Table 5

Correlation coefficients among relative values of traits in maize inbred lines"

项目Item 相对发芽势Relative germination potential 相对发芽率Relative germination rate 相对发芽指数Relative germination index 相对平均发芽时间Relative average time to germination 相对幼苗干重Relative dry weight of seedlings
相对发芽率 0.56
相对发芽指数 0.64 0.98**
相对平均发芽时间 -0.48 -0.55 -0.66
相对幼苗干重 0.59 0.94** 0.98** -0.75
相对活力指数 0.56 0.93** 0.96** -0.71 0.99**
[1] Cutforth H W, Shaykewich C F . A temperature response function for corn development. Agriculture and Forest Meteorology, 1990,50(3):159-171.
doi: 10.1016/0168-1923(90)90051-7
[2] 胡海军, 史振声, 王志斌 , 等. 抗低温处理对玉米种子萌发特性的影响.中国种业, 2009(5):44-45.
[3] 谭振波, 刘昕, 曹鸣庆 . 玉米抗寒性的研究进展. 玉米科学, 2002,10(20):56-60.
[4] Verheul M J, Picatto C, Stamp P . Growth and development of maize (Zea mays L.) seedlings under chilling conditions in the field. European Journal of Agronomy, 1996,5(1-2):31-43.
doi: 10.1016/S1161-0301(96)02007-2
[5] Hund A, Richner W, Soldati A , et al. Root morphology and photosynthetic performance of maize inbred lines at low temperature. European Journal of Agronomy, 2007,27(1):52-61.
doi: 10.1016/j.eja.2007.01.003
[6] Farooq M, Aziz T, Wahid A , et al. Chilling tolerance in maize:agronomic and physiological approaches. Crop and Pasture Science, 2009,60(6):501-516.
doi: 10.1071/CP08427
[7] 马延华, 王庆祥, 陈绍江 . 玉米耐寒性鉴定研究进展.作物杂志, 2012(4):1-8.
[8] 高素华, 郭建平, 王连敏 , 等. 低温对玉米种子发芽的影响. 气象, 1998,24(5):52-55.
[9] 马树庆, 袭祝香, 王琪 . 中国东北地区玉米低温冷害风险评估研究. 自然灾害学报, 2003,12(3):137-141.
[10] 朱海霞, 陈莉, 王秋京 , 等. 1980-2009 年期间黑龙江省玉米低温冷害年判定. 灾害学, 2012,27(1):44-47.
[11] 杨光, 刘宏魁, 李世鹏 , 等. 玉米抗冷种质资源的筛选与鉴定. 玉米科学, 2012,20(1):57-60,66.
[12] 张晓聪, 雍洪军, 张焕欣 , 等. 玉米芽期和苗期耐冷性研究进展.作物杂志, 2012(6):8-14.
[13] Bhosale S U, Rymen B , Beemster G T S, et al.Chilling tolerance of central European maize lines and their factorial crosses. Annals of Botany, 2007,100:1315-1321.
doi: 10.1093/aob/mcm215
[14] McConnell R L, Gardner C O . Selection for cold germination in two corn populations. Crop Science, 1979,19(6):765-768.
doi: 10.2135/cropsci1979.0011183X001900060004x
[15] 赵玉田, 胡荣海 . 玉米抗冷性鉴定II—筛选方法和指标.中国农业科学, 1986(2):18-22.
[16] Hodges D M, Hamilton R I, Charest C . A chilling resistance test for inbred maize lines. Canadian Journal of Plant Science, 1994,74(4):687-691.
doi: 10.4141/cjps94-124
[17] 李红飞, 郭薇, 覃光恒 , 等. 玉米耐低温种质资源的初步鉴定和筛选.中国种业, 2014(6):30-32.
[18] 郑昀晔, 胡晋, 张胜 , 等. 玉米自交系发芽期和苗期耐寒性的鉴定. 浙江大学学报(农业与生命科学版), 2006,32(1):41-45.
[19] Hope H J, White R P, Dwyer L M , et al. Low temperature emergence potential of short season corn hybrids grown under controlled environment and plot conditions. Canadian Journal of Plant Science, 1992,72(1):83-91.
doi: 10.4141/cjps92-009
[20] 扈光辉, 王天宇, 苏俊 , 等. 玉米种质苗期耐冷性状的遗传分析. 中国农学通报, 2009,25(6):101-106.
[21] 胡新民, 王建华 . 玉米种子萌发与幼苗期耐冷性鉴定研究. 中国作物学会学术年会论文摘要集, 2011: 32.
[22] 马延华, 王庆祥, 孙德全 , 等. 玉米自交系芽期耐寒性的鉴定与评价. 玉米科学, 2013,21(2):88-92.
[23] 肖永瑚 . 玉米不同生育期耐冷性研究. 作物学报, 1984,10(1):41-49.
[24] 扈光辉 . 耐冷玉米种质资源的筛选与鉴定. 杂粮作物, 2008,28(6):370-373.
[25] 张雪峰, 张立军, 胡滨 . 玉米萌发期种子耐低温鉴定指标的筛选. 辽宁农业科学, 2011(1):25-30.
[26] 赵玉田, 梁博文, 张晓玲 , 等. 我国寒冷地区玉米品种(系)抗冷性筛选原理与技术体系的应用研究. 中国农学通报, 1993,9(2):23-27.
[27] 李俊明 . 玉米自交系苗期耐冷性鉴定. 华北农学报, 1988,3(1):7-12.
doi: 10.3321/j.issn:1000-7091.1988.01.002
[28] 胡荣海, 赵玉田, 高吉寅 . 用质膜透性鉴定玉米苗期抗寒性.植物生理学通讯, 1981(6):35-37.
[29] 毕辛华, 戴心维 .种子学 .北京: 中国农业出版社, 1993: 79.
[30] 陈士林 . 玉米种子活力与田间苗期性状相关性研究.种子, 2003(4):35-37.
[31] Greaves J A . Improving suboptimal temperature tolerance in maize-the search for variation. Journal of Experimental Botany, 1996,47(3):307-323.
doi: 10.1093/jxb/47.3.307
[32] 马延华, 王庆祥, 陈绍江 . 玉米耐寒性生理生化机理与分子遗传研究进展. 玉米科学, 2013,21(3):76-81.
[33] Hodges D M, Andrews C J, Johnson D A , et al. Sensitivity of maize hybrids to chilling and their combining abilities at two developmental stages. Crop Science, 1997,37:850-856.
doi: 10.2135/cropsci1997.0011183X003700030026x
[34] Leipner J, Stamp P . Chilling stress in maize seedlings. Biomedical and Life Sciences, 2009,10:291-310.
[35] Revilla P, Malvar R A, Cartea M E , et al. Inheritance of cold tolerance at emergence and during early season growth in maize. Crop Science, 2000,40(6):1579-1585.
doi: 10.2135/cropsci2000.4061579x
[1] Wu Ruixiang, Yang Jianchun, Wang Liqin, Guo Xiujuan. Evaluation of the Adaptability of Flax Drought#br# Resistance Based on Multiple Statistics Analysis [J]. Crops, 2018, 34(5): 10-16.
[2] Chen Guangzhou, Wang Guangfu, Qu Jianzhou, Si Leiyong, . Study on Grain Dehydration Rate and#br# Correlation Analysis of Major Related#br# Characters in Different Maize Inbred Lines [J]. Crops, 2018, 34(5): 33-39.
[3] Wang Lei, Zhang Xiangping, Li Runxi, Niu Xiaoxia, . Multivariate Analysis and Evaluation on Agronomic#br# Traits and Grain Amylopectin Content of Barley [J]. Crops, 2018, 34(5): 71-76.
[4] Zhang Yizhong, Zhou Fuping, Zhang Xiaojuan, . Identification and Cluster Analysis of Photosynthetic#br# Characters and WUE in Sorghum Germplasm [J]. Crops, 2018, 34(5): 45-53.
[5] Su Guihua, Li Chunlei, Su Yichen. Evaluation of 22 Main Popularized Varieties#br# by Variety Regional Trails in Jilin Province [J]. Crops, 2018, 34(5): 63-70.
[6] Wu Ronghua, Zhuang Kezhang, Liu Peng, Zhang Chunyan. Response of Summer Maize Yield to#br# Meteorological Factors in Lunan Region [J]. Crops, 2018, 34(5): 104-109.
[7] Li Shaokun, Zhang Wanxu, Wang Keru, Han Dongsheng, . Study on Maize Mechanical Grain#br# Harvest in Northern Xinjiang [J]. Crops, 2018, 34(5): 127-131.
[8] Gao Wenjun, Yang Guoyi, Gao Xinzhong, Yu Zhu, . The Effects of Nitrogen, Phosphorus, or Potassium#br# Fertilizer on the Yield and Silage Quality of Maize [J]. Crops, 2018, 34(5): 144-149.
[9] Hongyan Li,Yonghong Wang,Rulang Zhao,Wenjie Zhang,Bo Ming,Ruizhi Xie,Keru Wang,Lulu Li,Shang Gao,Shaokun Li. The Construction and Application of Maize Grain Dehydration Model in Yellow River Irrigation and Pumping Irrigation District in Ningxia [J]. Crops, 2018, 34(4): 149-153.
[10] Xiaoyu Liang, Chunyu Lin, Shumei Ma, Yang Wang. Mining Elite Alleles for Germination Ability in Rice (Oryza sativa L.) under Salt and Alkaline Stress [J]. Crops, 2018, 34(4): 48-52.
[11] Shaokun Li,Wanxu Zhang,Keru Wang,Wanbing Yu,Yongsheng Chen,Dongsheng Han,Xiaoxia Yang,Chaowei Liu,Guoqiang Zhang,Yizhou Wang,Fenghe Liu,Jianglu Chen,Jingjing Yang,Ruizhi Xie,Peng Hou,Bo Ming. The Selection of High Yield Maize Cultivars Suitable for Dense Planting and Grain Mechanical Harvesting in North of Xinjiang [J]. Crops, 2018, 34(4): 62-68.
[12] Yanli Fan,Hui Dong,Baishan Lu,Yaxing Shi,Ning Gao,Yamin Shi,Li Xu,Shengli Xi,Cuifen Zhang,Yanhui Liu. Effects of Sowing Date on Starch Gelatinization Characteristics of Different Waxy Maize Varieties [J]. Crops, 2018, 34(4): 79-83.
[13] Shaokun Li,Keru Wang,Yanbo Wang,Haiyan Zhao,Yuzhong Shen,Dandan Cai,Wanxin Xiao,Wenye Jiang,Zhaofu Huang,Lichao Zhai,Ruizhi Xie,Peng Hou,Bo Ming. The Quality of Mechanical Harvesting Maize Grain and Its Influencing Factors in Central Liaoning Province [J]. Crops, 2018, 34(3): 162-167.
[14] Bin Zhang,Jinxiu Li,Zhen Wang,Hao Feng,Jinbang Li. Correlation and Cluster Analysis of Agronomic Traits in Wheat Lines [J]. Crops, 2018, 34(3): 57-60.
[15] Lei Shi,Guohong Wang,Yanbo Wang,Dawei Wang,Haiyan Zhao. Preliminary Study on Grain Dehydration Rate of Maize Hybrids and Their Parents [J]. Crops, 2018, 34(3): 84-89.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .