Crops ›› 2020, Vol. 36 ›› Issue (2): 20-27.doi: 10.16035/j.issn.1001-7283.2020.02.004

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Identification of Stearyl -ACP Desaturase Gene (SbSAD) Family and Their Expression Analysis at Different Developmental Stages in Sorghum

Zhao Xunchao,Xu Jingyu(),Gai Shengnan,Wei Yulei,Xu Xiaoxuan,Ding Dong,Liu Meng,Zhang Jinjie,Shao Wenjing   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University/Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, Daqing 163319, Heilongjiang, China
  • Received:2019-08-19 Revised:2019-10-14 Online:2020-04-15 Published:2020-04-13
  • Contact: Jingyu Xu E-mail:xujingyu2003@hotmail.com

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Abstract:

Stearyl-ACP desaturase is a key enzyme to form unsaturated fatty acids. A total of 11 full-length SbSADs genes were identified by Blastp from the NCBI and Phytozome database using the SbSADs genes as queries. The protein properties, phylogenetics, tissue-specific expression patterns of SbSADs, gene structure, conserved motifs, chromosome mapping, secondary and tertiary structures, cis-elements in promoter regions and their expression level at various seed development stages were analyzed. The results showed that open reading frame of SbSADs genes was among 1 134-1 290bp, the number of amino acids of their encoded proteins ranged from 377 to 449, the maximum molecular weight was 48.0kDa and the minimum was 39.8kDa, and the isoelectric point was 5.26-8.76. According to phylogenetic analysis, SbSADs were divided into 2 sub-groups, and the expression level of sub-group I was higher in different tissues. SbSADs genes were unequally mapped on 1, 2, 3, 4, 6, 7 and 10 chromosomes of sorghum. The prediction of secondary structure showed that SbSADs proteins was mainly composed of α-helix and irregular coil, while the tertiary structures prediction showed that except SbSAD8, the rest of SbSADs proteins were highly similar. In the SbSADs gene family, there were a number of cis-elements related to low temperature stress. The expression of SbSAD5 gene was higher at 5 and 10d of seed development.

Key words: Sorghum, Stearyl-ACP desaturase, Bioinformatics, Gene family

Table 1

Analysis of protein characterization of SbSADs in sorghum"

基因
Gene		 登录号
ID		 编码区(bp)
Open reading frame		 氨基酸数
Number of amino acid		 分子量(kDa)
Molecular weight		 等电点
Isoelectric point
SbSAD1 Sobic.001G265300 1 290 429 47.5 8.76
SbSAD2 Sobic.001G280700 1 194 397 44.7 7.15
SbSAD3 Sobic.001G334501 1 350 449 48.0 6.47
SbSAD4 Sobic.002G258500 1 245 414 44.5 6.79
SbSAD5 Sobic.003G377100 1 143 380 42.7 6.62
SbSAD6 Sobic.003G404000 1 194 397 45.1 6.44
SbSAD7 Sobic.004G153300 1 251 416 46.7 6.62
SbSAD8 Sobic.004G157550 1 134 377 39.8 5.26
SbSAD9 Sobic.006G048700 1 179 392 44.7 6.53
SbSAD10 Sobic.007G071600 1 272 423 46.9 8.18
SbSAD11 Sobic.010G235300 1 233 410 45.6 6.71

Fig.1

Phylogenetic relationships of SAD proteins from rice, maize, Arabidopsis and sorghum"

Fig.2

The expression profile of SbSADs gene family at different developmental stages and tissues in sorghum"

Fig.3

Genetic structure analysis of SbSAD gene family in sorghum"

Fig.4

Conserved motif analysis of SbSADs protein"

Fig.5

Chromosomal location of SbSADs"

Table 2

Amino acid number and percentage of secondary structure of SbSADs protein"

蛋白
Protein		 α-螺旋α-helix 延长链Extension chain β-折叠β-sheet 无规则卷曲Random coil
数量
Amount		 比例(%)
Proportion		 数量
Amount		 比例(%)
Proportion		 数量
Amount		 比例(%)
Proportion		 数量
Amount		 比例(%)
Proportion
SbSAD1 221 51.52 46 10.72 45 10.49 117 27.27
SbSAD2 206 51.89 40 10.08 29 7.30 122 30.73
SbSAD3 216 48.11 57 12.69 54 12.03 122 27.17
SbSAD4 212 51.21 49 11.84 38 9.18 115 27.78
SbSAD5 215 56.58 40 10.53 39 10.26 86 22.63
SbSAD6 210 52.90 49 12.34 29 7.30 109 27.46
SbSAD7 219 52.64 32 7.69 30 7.21 135 32.45
SbSAD8 153 40.58 54 14.32 24 6.37 146 38.73
SbSAD9 205 53.66 38 9.95 32 8.38 107 38.73
SbSAD10 225 53.19 51 12.06 39 9.22 108 25.53
SbSAD11 243 59.27 31 7.56 43 10.49 93 22.68

Fig.6

Prediction results of secondary structure (A) and tertiary structure (B) of SbSADs protein"

Fig.7

Distribution of cis-elements in SbSADs gene promoters"

Fig.8

Expression of SbSADs gene at different development days of seeds"

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