Crops ›› 2020, Vol. 36 ›› Issue (2): 112-118.doi: 10.16035/j.issn.1001-7283.2020.02.017

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Pollen Viability, Morphology and Physiological Indexes of Three Tobacco Varieties at Different Storage Temperatures

Pan Lei,Xu Jie,Yang Shuai,Chen Yunsong,Chen Lianhong,Ma Wenguang()   

  1. Yuxi Zhongyan Tobacco Seed Co.,Ltd, Yuxi 653100, Yunnan, China
  • Received:2019-09-02 Revised:2019-12-05 Online:2020-04-15 Published:2020-04-13
  • Contact: Wenguang Ma E-mail:2570315899@qq.com

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Abstract:

The tobacco varieties N. rustica, Maryland 609 and Yunyan 85 pollen were used as experimental materials to study the changes of pollen viability, morphology and related physiological indicator of tobacco varieties under different storage temperatures. Taking the tobacco pollen viability test results of N. rustica as an example, the pollen viability stored for 16 months at -196℃ and -80℃ was 0.64±0.06 and 0.65±0.09 respectively, and there was no significant decrease compared with that before storage (0.66±0.04). The pollen lost its vitality after 5 months storage at 4℃. Electron micrographs of the pollen stored under conditions of -196℃ and -80℃ showed that the pollen morphology was still full, while the pollens stored at 4℃ were shrunken and deformed, the germination grooves were distorted. Solule sugar content (309.276mg/g), soluble protein content (55.216mg/g), total free amino acid content (15.817μmol/mg), free proline content (1.596mg/g), SOD activity (4.284U/g) of pollen stored at -196℃ was significantly higher than that at 4℃. Pollen free proline content (1.626mg/g) and SOD activity (5.546U/g) stored at -80℃ were higher than that of -196℃. The difference maybe due to the freezing speed at different temperature conditions. The results showed that preservation at -196℃ and -80℃ were more favorable for preserving pollen viability of tobacco.

Key words: Tobacco, Pollen storage, Pollen viability, Pollen morphology, Total free amino acids, SOD activity

Table 1

Results of pollen vitality of the tested tobacco varieties under different storage temperature conditions"

检测时间
Time of detection		 -196℃ -80℃ 4℃
N. rustica Maryland
609		 云烟85
Yunyan 85		 N. rustica Maryland
609		 云烟85
Yunyan 85		 N. rustica Maryland
609		 云烟85
Yunyan 85
2017年2月 0.66±0.04a 0.66±0.07a 0.64±0.09a 0.65±0.06a 0.64±0.12a 0.63±0.08a 0.65±0.10a 0.63±0.07a 0.60±0.09a
2017年3月 0.64±0.06a 0.66±0.05a 0.63±0.06a 0.64±0.08a 0.65±0.05a 0.63±0.06a 0.56±0.13b 0.51±0.19b 0.48±0.06b
2017年4月 0.67±0.03a 0.65±0.05a 0.64±0.04a 0.65±0.05a 0.63±0.06a 0.64±0.04a 0.50±0.11b 0.48±0.16b 0.43±0.12b
2017年5月 0.65±0.09a 0.64±0.04a 0.64±0.10a 0.64±0.07a 0.63±0.06a 0.62±0.12a 0.18±0.04c 0.16±0.05c 0.12±0.04c
2017年6月 0.64±0.04a 0.66±0.11a 0.65±0.11a 0.66±0.07a 0.64±0.09a 0.64±0.11a 0.08±0.02d 0.03±0.00d 0.02±0.00d
2017年7月 0.67±0.05a 0.64±0.05a 0.63±0.10a 0.63±0.07a 0.64±0.07a 0.64±0.05a — — —
2017年8月 0.64±0.06a 0.64±0.03a 0.66±0.04a 0.65±0.02a 0.65±0.04a 0.63±0.04a — — —
2017年9月 0.66±0.04a 0.66±0.07a 0.65±0.02a 0.66±0.09a 0.64±0.06a 0.62±0.07a — — —
2017年10月 0.65±0.11a 0.65±0.02a 0.64±0.08a 0.64±0.04a 0.63±0.07a 0.64±0.07a — — —
2017年11月 0.66±0.08a 0.64±0.09a 0.62±0.06a 0.63±0.09a 0.65±0.07a 0.63±0.05a — — —
2017年12月 0.64±0.04a 0.65±0.10a 0.66±0.05a 0.65±0.04a 0.64±0.06a 0.65±0.06a — — —
2018年1月 0.67±0.05a 0.65±0.08a 0.63±0.05a 0.64±0.12a 0.65±0.08a 0.62±0.04a — — —
2018年2月 0.65±0.03a 0.62±0.10a 0.64±0.05a 0.66±0.08a 0.63±0.05a 0.65±0.07a — — —
2018年3月 0.65±0.09a 0.67±0.04a 0.64±0.07a 0.63±0.08a 0.64±0.05a 0.64±0.10a — — —
2018年4月 0.65±0.03a 0.64±0.05a 0.64±0.12a 0.66±0.05a 0.65±0.04a 0.64±0.04a — — —
2018年5月 0.67±0.04a 0.66±0.05a 0.62±0.10a 0.64±0.06a 0.63±0.08a 0.63±0.12a — — —
2018年6月 0.64±0.06a 0.65±0.08a 0.64±0.07a 0.65±0.09a 0.64±0.11a 0.64±0.05a — — —

Fig.1

Electron micrograph of tobacco pollen population under the different storage conditions"

Table 2

Soluble sugar content of tobacco pollen under the different storage temperatures mg/g"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 309.276±2.989a 287.652±4.180a 295.849±9.185a
-80℃ 286.808±8.476a 270.906±1.036a 272.807±3.455a
4℃ 158.056±8.484b 136.702±1.647b 128.197±4.926b

Table 3

Total amount of free amino acids in tobacco pollen under the different storage temperatures μmol/mg"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 15.817±2.136b 18.008±0.149b 17.121±0.480b
-80℃ 24.152±1.395a 26.194±1.505a 24.173±0.453a
4℃ 12.574±0.132c 12.386±0.245c 13.354±0.964c

Table 4

Proline content of tobacco pollen under the different storage temperatures mg/g"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 1.596±0.076a 1.608±0.026a 1.581±0.036a
-80℃ 1.626±0.061a 1.691±0.057a 1.673±0.028a
4℃ 1.328±0.053b 1.365±0.147b 1.352±0.140b

Table 5

Soluble protein content of tobacco pollen under the different storage temperatures mg/g"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 55.216±1.615a 55.633±2.847a 54.859±2.595a
-80℃ 53.001±1.605a 51.397±2.282a 51.718±2.228a
4℃ 42.410±2.072b 42.758±1.446b 42.100±2.560b

Table 6

Tobacco pollen SOD activity under the different storage temperatures U/g"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 4.284±0.208b 4.984±0.038b 4.344±0.419b
-80℃ 5.546±0.621a 7.154±0.771a 6.729±0.690a
4℃ 2.853±0.493c 2.644±0.079c 2.680±0.474c

Table 7

MDA content of tobacco pollen at the different storage temperatures μmol/g"

贮藏温度
Storage temperature		 N. rustica Maryland 609 云烟85
Yunyan 85
-196℃ 2.205±0.092b 2.305±0.132b 2.821±0.612b
-80℃ 2.250±0.168b 2.370±0.552b 2.529±0.215b
4℃ 8.134±0.349a 4.903±0.313a 4.744±0.247a
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