Crops ›› 2020, Vol. 36 ›› Issue (1): 47-54.doi: 10.16035/j.issn.1001-7283.2020.01.009

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Effects of EMS Mutagenesis on Emergence and Agronomic Traits in Sorghum

Fan Xinqi1,Wang Haiyan2,Nie Meng′en1,Zhao Xingkui1,Zhang Yizhong1,Yang Huiyong1,Zhang Xiaojuan1,Liang Du1,Duan Yonghong3,Liu Qingshan1()   

  1. 1Institute of Sorghum, Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Jinzhong 030600, Shanxi, China
    2Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, Shanxi, China
    3Agriculture College of Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2019-06-06 Revised:2019-11-20 Online:2020-02-15 Published:2020-02-23
  • Contact: Qingshan Liu E-mail:673673@163.com

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Abstract:

In order to study the effects of EMS treatment on seed germination, plant height, leaf, panicle type, fertility and mature period of sorghum and determine the optimal conditions of EMS treatment, the seeds of “Jinliang 5” and “V4B” were treated with different concentrations of EMS for various times. The results showed that the germination rate of Jinliang 5 and V4B increased with the increasing of EMS concentration and treatment time. Plant height aberration rate, fertility aberration rate and maturation period variation rate of two genotypes increased with the increasing of EMS concentration, while the affect of EMS treatment on leaf shape aberration rate and panicle type aberration rate of the two materials were different. When EMS was used for mutation breeding in sorghum, 0.20% EMS treatment for 12h was identified as a suitable condition for the construction of a mutant library of Jinliang 5 and V4B. 0.20% and 0.25% EMS treatment for 12h was recognized as the suitable conditions for screening plant height mutants of Jinliang 5 and V4B, respectively. 0.25% EMS treatment for 12h can be adopted as the suitable conditions for screening panicle type mutants of Jinliang 5 and V4B and screening leaf mutants of Jinliang 5, and 0.35% EMS for 12h can be adopted as the suitable conditions for screening maturity stage mutants of Jinliang 5 and V4B.

Key words: Sorghum (Sorghum bicolor L.), EMS mutation, Seedling emergence, Agronomic trait

Table 1

Effects of the different EMS treatments on emergence rates of Jinliang 5 and V4B %"

EMS浓度(%)
EMS
concentration		 12h 16h 20h
晋粱5号
Jinliang 5		 V4B
V4B		 种质间差异
Germplasm difference		 晋粱5号
Jinliang 5		 V4B
V4B		 种质间差异
Germplasm difference		 晋粱5号
Jinliang 5		 V4B
V4B		 种质间差异
Germplasm difference
0.00 80 82 80 82 81 82
0.20 85** 83 82 78* * 79 69** **
0.25 86** 78 ** 83 75** ** 79 65** **
0.30 86** 76* ** 85* 73** ** 77 64** **
0.35 83 73** ** 83 72** ** 74** 64** **
0.40 82 72** ** 81 71** ** 72** 64** **
0.45 79 71** ** 77 64** ** 73** 59** **
0.50 75** 64** ** 74** 62** ** 67** 54** **

Fig.1

Effects of different EMS treatments on relative seedling survival rate of sorghum "**" represents extremely significant difference (P<0.01), the same below"

Fig.2

Effects of different EMS treatments on plant height variation rate of sorghum"

Fig.3

Effects of different EMS treatments on plant fertility aberration rates of Jinliang 5 (a) and V4B (b)"

Fig.4

Effects of different EMS treatments on plant leaf shape, panicle type and mature period of sorghum"

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