Crops ›› 2017, Vol. 33 ›› Issue (2): 29-33.doi: 10.16035/j.issn.1001-7283.2017.02.005

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The Relationship and Cluster Analysis on Polysaccharides and Cellulose of Different Varieties of Dendrobium officinale

He Huyi,Tan Guanning,He Xinmin,Yang Xin,Tang Zhouping,Li Lishu   

  1. Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China
  • Received:2017-01-17 Revised:2017-02-10 Online:2017-04-15 Published:2018-08-26

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Abstract:

Dendrobium polysaccharide and cellulose content of different Dendrobium officinale varieties and the same variety in different altitudes were determined. The content of Dendrobium polysaccharide was determined by sulphuric acid phenol colorimetric method and the cellulose content was determined by drying method. The results showed that there were significant differences for Dendrobium polysaccharide and cellulose contents among different Dendrobium officinale varieties. The coefficient of variation of Dendrobium polysaccharide was less than that of cellulose, but PIC of Dendrobium polysaccharide was greater than that of cellulose. Dendrobium polysaccharide content was negatively correlated with the cellulose content. With the increase of altitude, Dendrobium polysaccharide content was increased but the cellulose content was decreased. Nine Dendrobium officinale varieties were clustered into two groups. One group was similar to Guangnan variety in Guiping city and another was similar to Duqiaoshan variety in Rongxian county.

Key words: Dendrobium officinale, Polysaccharides, Cellulose, Correlation analysis, Cluster analysis

Table 1

The evaluation standard of dendrobium polysaccharide and cellulose"

性状类型Character type 石斛多糖Polysaccharide 纤维素Cellulose
“1” <20.0% <1.00%
“2” 20.0%~24.9% 1.00%~1.99%
“3” 25.0%~29.9% 2.00%~2.99%
“4” 30.0%~34.9% 3.00%~3.99%
“5” 35.0%~39.9% 4.00%~4.99%
“6” 40.0%~44.9% 5.00%~5.99%
“7” >45.0% >6.00%

Table 2

Comparison of polysaccharide and cellulose content of different dendrobium varieties"

品种
Variety		 鲜茎Fresh stem 干茎Dry stem
石斛多糖Polysaccharide 纤维素Cellulose 石斛多糖Polysaccharide 纤维素Cellulose
桂斛2号Guihu 2 34.7% 2.26% 40.6% 6.16%
广南种Guangnanzhong 25.0% 2.14% 31.1% 4.07%
桂经斛003 Guijinghu 003 31.0% 2.13% 35.4% 6.88%
桂经斛002 Guijinghu 002 35.2% 1.27% 29.0% 3.13%
都峤山1号Duqiaoshan 1 26.0% 2.74% 44.7% 5.50%
都峤山2号Duqiaoshan 2 31.6% 1.88% 46.6% 5.80%
都峤山3号Duqiaoshan 3 30.8% 2.80% 46.2% 5.60%
桂斛1号（CK）Guihu 1 28.0% 1.96% 45.3% 3.78%
桂经斛001 Guijinghu 001 35.9% 1.58% 45.9% 3.30%
平均值Mean 30.9% 2.08% 40.5% 4.91%
标准差Standard deviation 0.0396 0.0049 0.0695 0.0136
变异系数Coefficient of variation (%) 12.82 23.70 17.14 27.69

Table 3

Polymorphism analysis of polysaccharide and cellulose from different dendrobium varieties"

性状类型
Character type		 鲜茎Fresh stem
石斛多糖
Polysaccharide		 纤维素
Cellulose
“1” - -
“2” - 4
“3” 3 5
“4” 4 -
“5” 2 -
“6” - -
“7” - -
样本数Sample size 9 9
性状类型数Character type number 3 2
PIC 0.642 0.494
对照类型Control type “3” “2”
对照类型Control type (%) 33.33 44.44

Table 4

Correlation analysis of polysaccharide and cellulose from different dendrobium varieties"

石斛多糖（鲜茎）
Polysaccharide (Fresh stem)		 纤维素（鲜茎）
Cellulose (Fresh stem)		 石斛多糖（干茎）
Polysaccharide (Dry stem)		 纤维素（干茎）
Cellulose (Dry stem)
石斛多糖（鲜茎）Polysaccharide (Fresh stem) 1.0000
纤维素（鲜茎）Cellulose (Fresh stem) -0.5583* 1.0000
石斛多糖（干茎）Polysaccharide (Dry stem) 0.0094 0.3967 1.0000
纤维素（干茎）Cellulose (Dry stem) -0.1127* 0.6256* 0.2138 1.0000

Table 5

Comparison of dendrobium polysaccharide and cellulose in Guijinghu 001 at different altitudes %"

海拔
Altitude (m)		 鲜茎Fresh stem 干茎Dry stem
石斛多糖
Polysaccharide		 纤维素
Cellulose		 石斛多糖
Polysaccharide		 纤维素
Cellulose
100 28.4b 2.89b 38.1b 6.06a
300 34.5a 2.37c 43.5a 5.88a
500 35.6a 1.61d 45.4a 5.34b
700 36.4a 1.50d 46.6a 5.12b
900 39.5a 1.32d 48.3a 4.38c
1100 23.4c 3.68a 37.6b 6.62a

Fig.1

Cluster analysis of different dendrobium varieties"

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