Crops ›› 2022, Vol. 38 ›› Issue (5): 141-145.doi: 10.16035/j.issn.1001-7283.2022.05.020

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Effects of Isosteviol on Growth of Wheat Seedlings under Salt Stress

Wang Yan(), Li Tingyou, Wang Dou, Li Jiawei, Peng Wenlu, Rui Haiyun()   

  1. Taizhou University, Taizhou 225300, Jiangsu, China
  • Received:2021-05-13 Revised:2021-09-13 Online:2022-10-15 Published:2022-10-19

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Abstract:

To study the influence of early seedling growth of wheat under salt stress by gibberellin structure analogues isosteviol, it is proposed to develop a new plant growth hormone which can improve the salt resistance of wheat. Using filter paper culture method, NaCl solution were simulated as the salt stress condition separately, 10-10~10-7mol/L isosteviol water solution were used to soak seeds of wheat, the control group CK (no salt stress) and model group (isosteviol soaking) were established. After seven days of growth, seedling length, root length, seedling fresh weight, root fresh weight, antioxidant enzymes (activities of SOD, CAT, POD) and content of MDA were measured. The results showed that the different contents of isosteviol played a certain role to improve the growth indicators and antioxidant enzyme activity of early wheat seedlings under salt stress, the most obvious role in promoting root growth and SOD activity. Under 100mmol/L NaCl salt stress condition, the best concentration of isosteviol for improving the salt resistance of wheat was 10-8mol/L. Compared with the model group, the root fresh weight of wheat seedlings increased by 89.7%, and SOD activity increased by 53.3%. Under 150mmol/L NaCl salt stress condition, the best concentration of isosteviol for improving the salt resistance of wheat was 10-9mol/L, compared with the model group, the root fresh weight of wheat seedlings increased by 80.8%, and SOD activity increased by 203.9%. In conclusion, isosteviol could relieve the stress of salt stress on early growth of wheat seedlings through improving the antioxidant enzyme activity, and the optimum concentration of isosteviol was different with the concentration of salt stress.

Key words: Isosteviol, Wheat, Salt stress, Antioxidant enzyme

Table 1

Factor levels"

处理Treatment NaCl (mmol/L) 异甜菊醇Isosteviol (mol/L)
A1 100 10-7
A2 100 10-8
A3 100 10-9
A4 100 10-10
模型组(A)
Model group (A)		 100 0
B1 150 10-7
B2 150 10-8
B3 150 10-9
B4 150 10-10
模型组(B)
Model group (B)		 150 0
CK 0 0

Table 2

Effects of isosteviol on growth of wheat seedlings under 100mmol/L NaCl salt stress"

处理
Treatment		 苗长
Seedling length (cm)		 根长
Root length (cm)		 苗鲜重
Seedling fresh weight (g)		 根鲜重
Root fresh weight (g)
CK 9.84±1.49 7.92±2.86 0.063±0.018 0.030±0.013
模型组(A) Model group (A) 7.80±1.226# 3.18±1.16## 0.061±0.013 0.029±0.006
A1 8.84±1.08* 7.12±1.14** 0.072±0.013 0.041±0.012*
A2 9.05±1.21* 6.56±1.58** 0.075±0.013 0.055±0.013**
A3 8.74±1.65* 7.76±1.06** 0.071±0.016 0.035±0.008*
A4 8.51±1.48* 5.85±1.22** 0.098±0.147 0.050±0.014**

Table 3

Effects of isosteviol on growth of wheat seedlings under 150mmol/L NaCl salt stress"

处理
Treatment		 苗长
Seedling length (cm)		 根长
Root length (cm)		 苗鲜重
Seedling fresh weight (g)		 根鲜重
Root fresh weight (g)
CK 9.84±1.49 7.92±2.86 0.063±0.018 0.030±0.013
模型组(B) Model group (B) 7.09±1.31# 2.93±0.70## 0.049±0.009# 0.026±0.007#
B1 7.06±0.92 3.94±0.70* 0.054±0.013 0.031±0.007*
B2 5.45±1.81 3.86±0.53* 0.045±0.012 0.031±0.011*
B3 7.37±1.33 4.29±0.52** 0.059±0.005* 0.047±0.012**
B4 5.54±1.17 2.95±0.35 0.038±0.013 0.030±0.007*

Fig.1

Effects of isosteviol on MDA content and SOD, POD, CAT activities of wheat leaves under 100mmol/L NaCl salt stress n=10, model group compared to CK group:“#”and“##”represent P < 0.05 and P < 0.01, respectively; experimental group compared to model group: “*”and“**”represent P < 0.05 and P < 0.01, respectively, the same below"

Fig.2

Effects of isosteviol on MDA content and SOD, POD, CAT activities of wheat leaves under 150mmol/L NaCl salt stress"

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