Crops ›› 2023, Vol. 39 ›› Issue (6): 41-46.doi: 10.16035/j.issn.1001-7283.2023.06.006

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Molecular Detection and Geographic Distribution of Stripe Rust Resistance Gene Loci in Yunnan Wheat Landraces

Chen Dan1(), Xiong Furong2(), Wu Shaoyun1, Bai Xiaodong3, Zhou Guoyan1, Wu Xiaoyang1, Cai Qing1()   

  1. 1Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southwest China, Ministry of Agriculture and Rural Affairs/Yunnan Key Laboratory of Agricultural Biotechnology/National Crop Germplasm Bank-Yunnan Branch,Kunming 650205, Yunnan, China
    2School of Biology and Chemistry, Pu'er University, Pu'er 665000, Yunnan,China
    3Wuding County Maojie Town Agricultural and Rural Service Center of Chuxiong Prefecture,Yunnan Province, Chuxiong 651607, Yunnan, China
  • Received:2022-07-25 Revised:2022-09-05 Online:2023-12-15 Published:2023-12-15

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Abstract:

The Yunnan wheat germplasm resources stored in the Yunnan Province Crop Germplasm Resources Bank are rich and diverse, and is a precious genetic resource for modern wheat breeding and improvement. Evaluation of exploration of the superior resistance genes from them are crucial steps in the enhancement of current wheat breeding. This study used molecular markers which were closely linked or co-isolated with resistance genes of Yr5, Yr10 and Yr18 to detect the gene loci in 208 yunnan wheat landrace, to clarify the distribution of stripe rust resistance gene loci of Yunnan wheat landrace, and provide theoretical reference for the selection and breeding of stripe rust resistance resources. The results showed that, Yr10 and Yr18 resistance genes were widely distributed in Yunnan wheat landrace, and a total of 143 test materials carried the two resistance genes above, accounting for 68.75%. Among them, the distribution frequency of Yr10 was the highest, with 104 materials carrying target fragments, there were 39 materials containing Yr18, however, Yr5 was less distributed, with only 12, accounting for 5.77%. At the same time, the geographical distribution of three disease resistance genes were studied. It was found that three resistance genes could be detected simultaneously in Baoshan Longyang District, Changning, Weishan, Fengqing and Lancang of Yunnan province. We speculate that this region might be the concentrated distribution region of Yr5, Yr10 and Yr18 genes.

Key words: Wheat, Landrace, Stripe rust resistance gene, Geographic distribution

Table 1

Molecular markers for detection of wheat stripe rust resistance genes"

基因
Gene		 标记名称
Marker name		 引物序列
Primer sequence		 连锁距离
Linkage distance (cM)		 退火温度
Annealing temperature (℃)		 文献
Reference
Yr5
 S1320
 F：CAATAGTTAGGCAAATTACATCG
R：GCTCAGTCAAACCGCTACTTCT		 0.3、0.0
 60
 [12]
Yr10
 SC200
 F：CTGCAGAGTGACATCATACA
R：TCGAACTAGTAGATGCTGGC		 0.5
 57
 [13]
Yr18
 csLV34
 F：GTTGGTTAAGACTGGTGATGG
R：TGCTTGCTATTGCTGAATAGT		 0.4、2.5
 60
 [14]

Table 2

Distribution of Yr5, Yr10 and Yr18 resistance genes in Yunnan wheat landraces"

编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip		 编号
Nunber		 Yr18 Yr10 Yr5 无带
No strip
1 + + 54 + 107 + 160 +
2 + 55 + 108 + 161 +
3 + 56 + 109 + 162 +
4 + 57 + 110 + 163 +
5 + 58 + 111 + 164 +
6 + 59 + 112 + 165 +
7 + 60 + 113 166 +
8 + 61 + 114 + + 167 +
9 + 62 + 115 + + 168 +
10 + + 63 + 116 + 169 +
11 + 64 + 117 + + 170 +
12 + 65 + 118 + 171 +
13 + 66 + 119 + 172 +
14 + 67 + 120 + 173 +
15 + 68 + 121 + + 174 +
16 + 69 + 122 + 175 +
17 + 70 + 123 + 176 +
18 + 71 + 124 + 177 +
19 + 72 + 125 + + 178 +
20 + 73 + 126 + 179 +
21 + 74 + 127 + 180 +
22 + 75 + 128 + 181 +
23 + 76 + 129 + 182 +
24 + 77 + 130 + 183 +
25 + 78 + 131 + 184 + +
26 + 79 + 132 + 185 + +
27 + 80 + 133 + + 186 +
28 + 81 + 134 + 187 +
29 + 82 + + 135 + 188 +
30 + 83 + 136 + 189 +
31 + 84 + 137 + 190 +
32 + 85 + 138 + + 191 +
33 + 86 + 139 + 192 +
34 + 87 + 140 + + 193 +
35 + 88 + 141 + 194 +
36 + 89 + 142 + 195 +
37 + 90 + 143 + 196 +
38 + 91 + 144 + 197 +
39 + 92 + 145 + 198 +
40 + 93 + + + 146 + 199 +
41 + 94 + 147 + 200 +
42 + 95 + 148 + 201 +
43 + 96 + 149 + 202 +
44 + 97 + 150 + 203 +
45 + 98 + 151 + 204 +
46 + 99 + 152 + 205 +
47 + 100 + 153 + 206 +
48 + 101 + 154 + 207 +
49 + 102 + 155 + 208 +
50 103 + 156 + Avocet S - - -
51 + + 104 + 157 + 阳性对照
Positive control		 + + +
52 + 105 + 158 +
53 + 106 + 159 +

Table 3

Distribution proportion of Yr5, Yr10 and Yr18 resistance genes in Yunnan wheat landraces"

项目Item Yr5 Yr10 Yr18 Yr18+Yr10 Yr18+Yr5 Yr10+Yr5 Yr18+Y10+Yr5 无带No strip
携带目标条带品种数量
Number of landraces with target bands		 12 104 39 5 6 3 1 67
携带目标条带品种占比
Percentage of the landraces with target bands (%)		 5.77 50.00 18.75 2.40 2.88 1.44 0.48 32.21

Fig.1

Detection results of molecular markers of Yr5, Yr10 and Yr18 resistance genes M: DL2000 marker; (a) 1-22 are part of samples, 23-24 are negative control Avocet S, 25 is positive control Avocet S*6/Yr5; (b) 1-28 are part of samples, 29-30 are negative control Avocet S, 31 is positive control Avocet S*6/Yr10; (c) 1-26 are part of samples, 27 is negative control Avocet S, 28-29 are positive control Avocet S*6/Yr18"

Table 4

Statistics on the number of Yunnan wheat landraces carrying resistance genes"

收集地
Select place		 Yr5 Yr10 Yr18 Yr18+
Yr10		 Yr10+
Yr5		 Yr18+
Yr5		 Yr18+
Yr10+Yr5
保山市隆阳区
Longyang District, Baoshan		 1 7 4 0 0 1 0
昌宁Changning 1 1 3 0 0 0 1
龙陵Longling 1 1 0 0 0 0 0
腾冲Tengchong 0 1 0 0 0 0 0
宾川Binchuan 0 0 0 0 0 0 0
剑川Jianchuan 0 0 1 0 0 0 0
南涧Nanjian 0 0 0 0 0 0 0
巍山Weishan 2 3 2 1 1 1 0
云龙Yunlong 0 0 1 0 0 0 0
德钦Deqin 0 0 0 0 0 0 0
维西Weixi 0 5 1 1 0 0 0
香格里拉
Shangri-La		 1 0 2 0 0 1 0
石屏Shiping 0 0 0 0 0 0 0
晋宁Jinning 0 1 2 1 0 0 0
永胜Yongsheng 0 1 0 0 0 0 0
凤庆Fengqing 1 24 8 0 0 1 0
耿马Gengma 0 2 0 0 0 0 0
临沧Lincang 0 4 2 0 0 0 0
双江Shuangjiang 0 8 0 0 0 0 0
永德Yongde 3 20 0 0 2 0 0
云县Yunxian 0 9 4 0 0 0 0
镇康Zhenkang 0 9 1 1 0 0 0
贡山Gongshan 0 1 0 0 0 0 0
兰坪Lanping 1 0 1 0 0 1 0
澜沧Lancang 1 6 4 0 0 1 0
普洱Pu?er 0 1 2 1 0 0 0
曲靖市麒麟区
Qilin District, Qujing		 0 0 0 0 0 0 0
玉溪红塔区
Hongta District, Yuxi		 0 0 1 0 0 0 0
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