水杨酸浸种对盐胁迫藜麦种子萌发及幼苗生长的影响

doi:10.16035/j.issn.1001-7283.2025.01.024

摘要/Abstract

摘要：

为了探究水杨酸浸种对盐胁迫抑制藜麦种子萌发和幼苗生长的缓解机制，以藜麦品种陇藜1号（盐敏感型）和陇藜4号（耐盐型）为试验材料，研究不同浓度（0、0.10、0.25、0.50、1.00 mmol/L）水杨酸浸种对中度盐胁迫（200 mmol/L NaCl）下藜麦种子萌发和幼苗生长及生理的影响。结果表明，2个藜麦品种在盐胁迫下种子发芽率均显著下降，幼苗生长受到抑制。经水杨酸处理后，盐胁迫引起的负面影响得到明显缓解，发芽率、发芽势以及发芽指数在浸种处理后均显著提高，有效促进了种子发芽。此外，2个藜麦品种幼苗的生长参数、地上部干重、抗氧化酶活性、渗透调节物质、叶绿素含量和根系活力均随水杨酸浸种浓度的增加呈先升后降的变化趋势，MDA含量则随水杨酸浸种浓度的增加先降后升，其中0.25 mmol/L水杨酸浸种浓度对盐胁迫下陇藜1号调控效果最佳，0.10 mmol/L对陇藜4号调控效果最佳。

关键词: 藜麦, 水杨酸浸种, 盐胁迫, 种子萌发, 幼苗生长

Abstract:

In order to investigate the alleviating mechanism of seed soaking with salicylic acid on salt-induced inhibition of seed germination and seedling growth in quinoa (Chenopodium quinoa Willd.), Longli 1 (salt- sensitive) and Longli 4 (salt-tolerant) were used as experimental materials to study the effects of different concentrations (0, 0.10, 0.25, 0.50, 1.00 mmol/L) of salicylic acid on seed germination, seedling growth, and physiology under 200 mmol/L NaCl moderate salt stress. The results showed that the seed germination rates of both quinoa varieties significantly decreased under salt stress, and the growth of seedlings were inhibited. After treatmenting with salicylic acid, the negative effects caused by salt stress were significantly alleviated, the germination rates, germination vigor, and germination indexes were significantly increased after soaking treatments, and effectively promoted seed germination. Furthermore, the seedling growth parameters of two quinoa varieties, aboveground dry weight, antioxidant enzyme activity, osmoregulatory substances, chlorophyll content, and root activity all exhibited a trend of first rising and then falling as the salicylic acid soaking concentration increased. The MDA content first decreased and then increased with the increasing of salicylic acid soaking concentration. It was discovered that the optimal concentration of 0.25 mmol/L salicylic acid for seed soaking had the best regulatory effect on Longli 1 under salt stress, while the optimal concentration of 0.10 mmol/L salicylic acid for seed soaking had the best regulatory effect on Longli 4 under salt stress.

Key words: Quinoa, Seed soaking with salicylic acid, Salt stress, Seed germination, Seedling growth

景茂雅, 张子玉, 张萌, 合佳敏, 严翻翻, 高艳梅, 张永清. 水杨酸浸种对盐胁迫藜麦种子萌发及幼苗生长的影响[J]. 作物杂志, 2025 (1): 194–201

Jing Maoya, Zhang Ziyu, Zhang Meng, He Jiamin, Yan Fanfan, Gao Yanmei, Zhang Yongqing. Effects of Seed Soaking with Salicylic Acid on Seed Germination and Seedling Growth of Quinoa under Salt Stress[J]. Crops, 2025(1): 194–201

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品种 Variety	处理 Treatment	发芽率 Germination rate (%)	发芽势 Germination vigor (%)	发芽指数 Germination index
L1	CK	92.2±0.0ab	88.7±0.0a	68.2±3.6a
	S1	85.0±0.0de	82.3±0.0b	59.3±4.4a
	S2	88.9±0.0bc	86.7±0.0a	61.8±3.9a
	S3	92.7±0.0a	90.7±0.0a	73.6±5.9a
	S4	87.8±0.0cd	87.3±0.0a	60.8±1.6a
	S5	83.9±0.0e	81.0±0.0b	58.3±1.4a
L4	CK	95.6±0.0a	92.7±0.0a	72.2±1.6a
	S1	88.9±0.0b	87.7±0.0b	60.2±1.1b
	S2	96.1±0.0a	93.3±0.0a	72.7±4.6a
	S3	91.7±0.0b	89.3±0.0b	68.5±1.9ab
	S4	88.9±0.0b	87.3±0.0b	59.0±0.6b
	S5	80.0±0.0c	79.0±0.0c	47.9±1.4c

品种Variety	处理Treatment	株高Plant height	根长Root length
L1	CK	20.5±0.3a	11.3±0.4b
	S1	17.1±0.7b	13.3±0.3ab
	S2	20.1±0.3a	14.5±0.5a
	S3	21.4±0.6a	15.7±0.3a
	S4	19.3±0.5ab	14.4±1.1a
	S5	17.1±1.0b	11.6±0.3b
L4	CK	23.0±0.9a	8.3±0.2d
	S1	18.3±0.7b	10.7±0.3c
	S2	23.3±0.6a	13.6±0.5a
	S3	21.2±0.7ab	12.2±0.2b
	S4	18.1±1.1b	10.5±0.4c
	S5	12.5±0.6c	7.1±0.4d