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作物杂志,2026, 第3期: 171–184 doi: 10.16035/j.issn.1001-7283.2026.03.023

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

亚麻bHLH转录因子基因家族鉴定与表达分析

唐立郦1(), 杨洌1, 张文洁3, 宋喜霞1, 程莉莉1, 刘丹丹1, 姚丹丹1, 姜卫东1, 康庆华1, 樊超2, 袁红梅1()   

  1. 1 黑龙江省农业科学院经济作物研究所， 150086， 黑龙江哈尔滨
    2 黑龙江省农业科学院耕作栽培研究所， 150001， 黑龙江哈尔滨
    3 黑龙江大学现代农业与生态环境学院， 150080， 黑龙江哈尔滨
  • 收稿日期:2025-02-21 修回日期:2025-04-14 出版日期:2026-06-15 发布日期:2026-06-17
  • 通讯作者: 袁红梅，主要从事亚麻遗传育种研究，E-mail：yuanhm1979@163.com
  • 作者简介:唐立郦，主要从事麻类作物遗传育种研究，E-mail：tanglili19861126@126.com
  • 基金资助:
    黑龙江省省属科研业务费(CZKYF2022-1-B026);黑龙江省农业科技创新跨越工程农业科技基础创新优青项目(CX23YQ01);国家麻类产业技术体系(CARS-16-S3)

Identification and Expression Analysis of bHLH Transcription Factor Gene Family in Flax (Linum usitatissimum L.)

Tang Lili1(), Yang Lie1, Zhang Wenjie3, Song Xixia1, Cheng Lili1, Liu Dandan1, Yao Dandan1, Jiang Weidong1, Kang Qinghua1, Fan Chao2, Yuan Hongmei1()   

  1. 1 Institute of Industrial Crops of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2 Institute of Tillage and Cultivation of Heilongjiang Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, China
    3 College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2025-02-21 Revised:2025-04-14 Online:2026-06-15 Published:2026-06-17

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摘要：

bHLH（basic Helix-Loop-Helix）是一类成员较多的转录因子，参与调控植物生长发育、形态建成和环境胁迫应答等过程。基于亚麻基因组数据库，利用生物信息学在亚麻基因组水平上对bHLH转录因子家族成员的染色体定位、理化性质、保守基序、保守结构域、基因结构、启动子顺势作用元件、进化关系及不同发育期茎中部的表达模式等进行分析。结果表明，亚麻bHLH基因家族有159个成员，不均匀地分布在15条染色体中。基因保守性分析表明，Motif 1和Motif 2（bHLH超家族）是亚麻bHLH家族中保守的蛋白序列。启动子顺式作用元件预测显示，亚麻bHLH基因家族在光响应、植物激素、逆境胁迫、生长发育以及次级代谢产物合成方面均发挥重要功能。进化分析表明，亚麻的159个成员被分成16个亚家族，与拟南芥、陆地棉和毛果杨的共线性分析发现，亚麻与陆地棉的共线性基因最多，推测可能源于共同祖先。RNA-seq分析表明，bHLH家族成员在亚麻不同发育时期的茎中部都有表达，其中LusbHLH101基因表达量最高。

关键词: 亚麻, bHLH基因家族, 表达分析, 转录因子

Abstract:

The basic Helix-Loop-Helix (bHLH) is a class of transcription factors that are involved in the regulation of plant growth and development, morphogenesis, and response to environmental stress. Based on the flax (Linum usitatissimum L.) genome database, this study used bioinformatics methods to analyze the chromosome localization, physicochemical properties, conserved motifs, conserved domains, gene structure, promoter cis-acting elements, phylogeretic relationships, and expression patterns in the middle stem at different developmental stages of the bHLH transcription factor family. The results showed that there were 159 members of the flax bHLH gene family, which were unevenly distributed among 15 chromosomes. Gene conservation analysis indicated that Motif 1 and Motif 2 (bHLH superfamily) were the conserved protein sequences in the flax bHLH family. The prediction of promoter cis-acting elements revealed that the flax bHLH gene family played important roles in light response, plant hormones, stress responses, growth and development, and secondary metabolism synthesis. Evolutionary analysis showed that the 159 members of flax were divided into 16 sub-families. The collinearity analysis with Arabidopsis thaliana, Gossypium hirsutum, and Populus trichocarpa revealed that flax had the most collinear genes with G. hirsutum, suggesting a possible common ancestor. RNA-seq analysis indicated that the bHLH family members were expressed at different developmental stages in the middle part of the flax stem, and the LusbHLH101 gene had the highest expression level.

Key words: Flax, bHLH gene family, Expression analysis, Transcription facctor

表1

qRT-PCR分析的基因和引物

基因名称Gene name 上游序列（5’-3’）Upstream sequence (5’-3’) 下游序列（5’-3’）Downstream sequence (5’-3’)
LusbHLH2 GTTTGTGGCATTACTGACT CTACGTTGTATAGGCTTGG
LusbHLH13 TGTTTCAAGGAGGAGGGAT GCTATGGAGGCTGAGGATA
LusbHLH25 CGCCGGACCTTGAGAAG GGGTTTCATCGGAAATGG
LusbHLH46 GCGGGCTCATTTGTCCT CCGATTCCCAGATTCCA
LusbHLH56 GGTGGCATGTGGGAGCT GAGGAATGCTGGGAGGTG
LusbHLH87 CGCAGCAGGGCAAGGTA GGGTTGTGAGCCAGCATT
LusbHLH98 ACCTGATTTGGGCGGATTA CGAGCCTTGTGATGTTGTTG
LusbHLH112 GTTCTTGACGAGATGGTGGCT TCCCGATAATACCTAACATACTTGC
LusbHLH126 GACCATAGATCCGAGAACCG CCTGCGTAGTACCGTAGCG
LusbHLH143 TAAAGAGGCAGTGGAAAGG TGTTCTACCAACGCTATGAG
LuGAPDH AGGTTCTTCCCGCTCTCAAT CCTCCTTGATAGCAGCCTTG

表2

基本理化性质和亚细胞定位分析

基因ID
Gene ID		 基因名称
Gene
name		 氨基酸数目
Amino acid
number		 分子量
Molecular
weight (Da)		 等电点
Isoelectric
point		 不稳定系数
Instability
index		 蛋白亲疏水平均值
Grand average
of hydropathicity		 亚细胞定位
Subcelluar
location
L.us.o.m.scaffold0.136 LusbHLH1 218 24 547.48 5.90 63.88 -0.928 细胞核
L.us.o.m.scaffold0.470 LusbHLH2 276 31 289.10 6.37 51.45 -0.800 细胞核
L.us.o.m.scaffold1.384 LusbHLH3 420 45 464.91 6.64 55.42 -0.579 细胞核
L.us.o.m.scaffold1.73 LusbHLH4 383 42 440.93 5.75 53.99 -0.796 细胞核
L.us.o.m.scaffold10.31 LusbHLH5 474 51 449.70 8.62 63.74 -0.558 细胞核
L.us.o.m.scaffold10.338 LusbHLH6 499 52 540.36 6.35 57.60 -0.614 细胞核
L.us.o.m.scaffold102.186 LusbHLH7 328 35 189.21 8.64 62.24 -0.735 细胞核
L.us.o.m.scaffold103.98 LusbHLH8 279 31 153.50 6.87 47.63 -0.777 细胞核
L.us.o.m.scaffold105.29 LusbHLH9 352 38 962.25 5.60 58.24 -0.646 细胞核
L.us.o.m.scaffold106.197 LusbHLH10 260 28 734.83 6.47 45.82 -0.848 细胞核
L.us.o.m.scaffold106.64 LusbHLH11 94 10 660.15 9.61 77.23 -0.501 细胞核
L.us.o.m.scaffold107.103 LusbHLH12 275 29 901.92 6.55 59.73 -0.318 细胞核
L.us.o.m.scaffold109.135 LusbHLH13 429 47 086.38 9.12 49.02 -0.548 细胞核
L.us.o.m.scaffold110.167 LusbHLH14 263 28 768.11 9.05 65.79 -0.186 细胞核
L.us.o.m.scaffold110.26 LusbHLH15 271 30 912.54 6.25 49.00 -0.710 细胞核
L.us.o.m.scaffold111.39 LusbHLH16 594 65 546.62 6.35 49.28 -0.503 细胞核
L.us.o.m.scaffold111.77 LusbHLH17 465 50 611.89 5.43 52.84 -0.357 细胞核
L.us.o.m.scaffold115.108 LusbHLH18 238 26 757.44 4.67 64.59 -0.172 细胞核
L.us.o.m.scaffold116.31 LusbHLH19 238 26 551.43 8.32 76.85 -0.288 细胞核
L.us.o.m.scaffold12.60 LusbHLH20 352 36 868.14 6.15 61.88 -0.576 细胞核
L.us.o.m.scaffold127.80 LusbHLH21 236 26 831.88 5.99 46.87 -0.810 细胞核
L.us.o.m.scaffold128.19 LusbHLH22 390 43 903.48 6.33 52.71 -0.632 细胞核
L.us.o.m.scaffold13.155 LusbHLH23 345 36 703.09 5.15 60.35 -0.561 细胞核
L.us.o.m.scaffold13.327 LusbHLH24 256 28 119.51 9.51 50.04 -0.523 细胞核
L.us.o.m.scaffold13.40 LusbHLH25 238 27 057.31 6.08 49.00 -0.699 细胞核
L.us.o.m.scaffold13.522 LusbHLH26 350 38 104.69 6.45 59.80 -0.347 细胞核
L.us.o.m.scaffold132.31 LusbHLH27 299 31 991.03 5.72 52.74 -0.389 细胞核
L.us.o.m.scaffold133.90 LusbHLH28 298 33 201.31 6.20 61.09 -0.854 细胞核
L.us.o.m.scaffold135.56 LusbHLH29 282 31 228.42 6.36 58.73 -0.351 细胞核
L.us.o.m.scaffold137.133 LusbHLH30 372 39 919.34 5.72 66.10 -0.583 细胞核
L.us.o.m.scaffold139.24 LusbHLH31 606 68 790.38 5.20 47.22 -0.716 细胞核
L.us.o.m.scaffold14.229 LusbHLH32 344 38 920.47 5.87 63.12 -0.626 细胞核
L.us.o.m.scaffold14.357 LusbHLH33 383 41 128.15 6.32 53.02 -0.495 细胞核
L.us.o.m.scaffold14.381 LusbHLH34 268 30 592.29 8.85 47.47 -0.425 细胞核
L.us.o.m.scaffold140.126 LusbHLH35 301 33 233.15 6.53 65.40 -0.447 细胞核
L.us.o.m.scaffold148.77 LusbHLH36 350 38 667.67 6.16 71.72 -0.461 细胞核
L.us.o.m.scaffold149.54 LusbHLH37 474 53 355.79 5.65 63.17 -0.659 细胞核
L.us.o.m.scaffold15.217 LusbHLH38 219 24 152.19 8.68 57.69 -0.448 细胞核
L.us.o.m.scaffold151.121 LusbHLH39 321 35 316.79 8.93 60.51 -0.451 细胞核
L.us.o.m.scaffold155.57 LusbHLH40 260 28 977.78 4.75 77.12 -0.364 细胞核
L.us.o.m.scaffold157.134 LusbHLH41 240 27 537.77 6.07 59.70 -0.388 细胞核
L.us.o.m.scaffold159.41 LusbHLH42 268 29 753.25 9.11 53.54 -0.259 细胞核
L.us.o.m.scaffold159.46 LusbHLH43 325 36 251.01 6.63 52.18 -0.811 细胞核
L.us.o.m.scaffold16.399 LusbHLH44 325 35 197.59 6.29 63.64 -0.736 细胞核
L.us.o.m.scaffold16.89 LusbHLH45 399 42 964.91 6.67 65.06 -0.581 细胞核
L.us.o.m.scaffold163.73 LusbHLH46 299 32 054.07 5.82 51.90 -0.425 细胞核
L.us.o.m.scaffold167.57 LusbHLH47 349 36 520.76 6.30 59.86 -0.552 细胞核
L.us.o.m.scaffold17.76 LusbHLH48 511 57 346.01 5.41 59.04 -0.695 细胞核
L.us.o.m.scaffold170.107 LusbHLH49 364 40 248.14 6.28 45.74 -0.550 细胞核
L.us.o.m.scaffold170.49 LusbHLH50 311 34 202.41 6.45 50.76 -0.577 细胞核
L.us.o.m.scaffold18.139 LusbHLH51 609 66 368.93 8.53 58.93 -0.598 细胞核
L.us.o.m.scaffold18.253 LusbHLH52 448 49 854.72 9.01 45.99 -0.625 细胞核
L.us.o.m.scaffold184.9 LusbHLH53 222 24 815.29 9.67 60.66 -0.529 细胞核
L.us.o.m.scaffold186.12 LusbHLH54 350 39 218.02 4.77 57.17 -0.781 细胞核
L.us.o.m.scaffold196.37 LusbHLH55 305 33 769.48 6.30 59.97 -0.544 细胞核
L.us.o.m.scaffold2.337 LusbHLH56 640 70 012.53 5.60 51.53 -0.726 细胞核
L.us.o.m.scaffold204.55 LusbHLH57 258 28 141.84 8.44 62.55 -0.580 细胞核
L.us.o.m.scaffold207.31 LusbHLH58 305 34 515.80 6.89 65.31 -0.702 细胞核
L.us.o.m.scaffold207.55 LusbHLH59 354 39 778.00 8.90 56.70 -0.872 细胞核
L.us.o.m.scaffold210.51 LusbHLH60 359 39 769.62 5.87 68.02 -0.523 细胞核
L.us.o.m.scaffold212.33 LusbHLH61 281 30 541.55 6.89 57.69 -0.370 细胞核
L.us.o.m.scaffold214.79 LusbHLH62 285 31 511.70 9.03 72.31 -0.563 细胞核
L.us.o.m.scaffold22.216 LusbHLH63 379 40 775.84 6.32 52.97 -0.462 细胞核
L.us.o.m.scaffold22.257 LusbHLH64 265 30 280.80 8.35 51.65 -0.435 细胞核
L.us.o.m.scaffold22.44 LusbHLH65 356 40 677.44 6.18 69.34 -0.675 细胞核
L.us.o.m.scaffold220.29 LusbHLH66 376 40 382.21 5.50 52.88 -0.583 细胞核
L.us.o.m.scaffold220.35 LusbHLH67 186 21 230.57 8.68 48.50 -0.383 细胞核
L.us.o.m.scaffold23.363 LusbHLH68 376 41 667.04 6.00 52.26 -0.813 细胞核
L.us.o.m.scaffold232.31 LusbHLH69 321 35 272.72 9.24 60.58 -0.485 细胞核
L.us.o.m.scaffold247.72 LusbHLH70 179 19 672.51 9.46 38.17 -0.555 细胞核
L.us.o.m.scaffold25.237 LusbHLH71 242 26 729.09 5.44 48.03 -0.677 细胞核
L.us.o.m.scaffold255.14 LusbHLH72 315 34 014.11 6.13 50.16 -0.532 细胞核
L.us.o.m.scaffold259.54 LusbHLH73 94 10 660.15 9.61 77.23 -0.501 细胞核
L.us.o.m.scaffold26.106 LusbHLH74 361 38 763.47 6.76 56.44 -0.720 细胞核
L.us.o.m.scaffold26.19 LusbHLH75 137 15 209.46 8.82 45.70 -0.402 细胞核
L.us.o.m.scaffold262.61 LusbHLH76 337 36 414.53 4.80 49.77 -0.351 细胞核
L.us.o.m.scaffold264.3 LusbHLH77 349 38 248.95 6.14 47.28 -0.841 细胞核
L.us.o.m.scaffold27.89 LusbHLH78 365 39 751.42 6.19 60.27 -0.768 细胞核
L.us.o.m.scaffold272.32 LusbHLH79 466 51 091.39 6.06 44.91 -0.407 细胞核
L.us.o.m.scaffold272.6 LusbHLH80 311 33 507.24 6.71 49.03 -0.233 细胞核
L.us.o.m.scaffold275.39 LusbHLH81 521 55 952.27 7.29 73.13 -0.564 细胞核
L.us.o.m.scaffold279.45 LusbHLH82 741 81 405.73 6.11 41.96 -0.381 细胞核
L.us.o.m.scaffold28.151 LusbHLH83 283 31 581.33 9.99 32.56 -0.457 细胞核
L.us.o.m.scaffold29.181 LusbHLH84 350 38 925.12 6.26 64.99 -0.607 细胞核
L.us.o.m.scaffold3.283 LusbHLH85 354 38 257.74 6.43 66.62 -0.731 细胞核
L.us.o.m.scaffold3.349 LusbHLH86 91 10 353.64 9.75 86.83 -0.655 细胞核
L.us.o.m.scaffold3.357 LusbHLH87 242 26 755.17 5.57 47.48 -0.660 细胞核
L.us.o.m.scaffold3.531 LusbHLH88 434 48 281.03 4.67 49.53 -0.267 细胞核
L.us.o.m.scaffold30.299 LusbHLH89 243 27 626.63 8.55 62.31 -0.516 细胞核
L.us.o.m.scaffold304.4 LusbHLH90 358 39 184.01 6.50 52.70 -0.756 细胞核
L.us.o.m.scaffold31.12 LusbHLH91 244 26 916.44 7.71 49.28 -0.443 细胞核
L.us.o.m.scaffold31.254 LusbHLH92 519 58 717.08 5.33 54.98 -0.446 细胞核
L.us.o.m.scaffold31.364 LusbHLH93 315 34 962.33 5.57 53.57 -0.755 细胞核
L.us.o.m.scaffold32.239 LusbHLH94 627 70 296.82 5.29 44.47 -0.369 细胞核
L.us.o.m.scaffold34.120 LusbHLH95 322 35 893.86 5.53 54.21 -0.839 细胞核
L.us.o.m.scaffold34.315 LusbHLH96 1279 141 272.27 6.64 48.86 -0.355 细胞核
L.us.o.m.scaffold34.340 LusbHLH97 406 45 049.06 7.07 47.69 -0.101 细胞核
L.us.o.m.scaffold35.64 LusbHLH98 258 28 750.26 7.99 51.68 -0.756 细胞核
L.us.o.m.scaffold358.10 LusbHLH99 366 40 492.16 9.96 63.35 -0.840 细胞核
L.us.o.m.scaffold359.19 LusbHLH100 356 39 261.87 6.10 47.25 -0.629 细胞核
L.us.o.m.scaffold36.149 LusbHLH101 204 23 029.64 8.85 53.66 -0.311 细胞核
L.us.o.m.scaffold39.76 LusbHLH102 347 38 707.70 5.26 62.51 -0.642 细胞核
L.us.o.m.scaffold40.160 LusbHLH103 260 27 397.64 6.03 44.13 -0.258 细胞核
L.us.o.m.scaffold41.302 LusbHLH104 550 59 525.87 8.89 52.29 -0.838 细胞核
L.us.o.m.scaffold43.153 LusbHLH105 982 109 089.25 9.47 51.42 -0.341 细胞核
L.us.o.m.scaffold44.105 LusbHLH106 208 23 410.55 5.89 57.26 -0.795 细胞核
L.us.o.m.scaffold44.71 LusbHLH107 179 20 111.13 9.59 32.85 -0.673 细胞核
L.us.o.m.scaffold46.278 LusbHLH108 473 51 335.28 8.18 67.54 -0.627 细胞核
L.us.o.m.scaffold48.120 LusbHLH109 215 23 916.45 7.22 54.02 -0.701 细胞核
L.us.o.m.scaffold48.86 LusbHLH110 279 30 353.37 9.43 43.35 -0.886 细胞核
L.us.o.m.scaffold49.55 LusbHLH111 369 40 483.75 5.69 53.60 -0.743 细胞核
L.us.o.m.scaffold56.162 LusbHLH112 234 25 922.41 7.71 50.46 -0.701 细胞核
L.us.o.m.scaffold56.317 LusbHLH113 554 60 089.04 5.54 53.05 -0.594 细胞核
L.us.o.m.scaffold57.183 LusbHLH114 499 53 627.70 5.99 55.31 -0.689 细胞核
L.us.o.m.scaffold6.478 LusbHLH115 405 45 582.32 7.16 65.90 -0.872 细胞核
L.us.o.m.scaffold6.496 LusbHLH116 211 24 107.43 9.17 67.46 -0.830 细胞核
L.us.o.m.scaffold60.268 LusbHLH117 258 28 602.08 7.93 51.32 -0.713 细胞核
L.us.o.m.scaffold61.53 LusbHLH118 266 29 064.23 6.24 59.86 -0.709 细胞核
L.us.o.m.scaffold63.210 LusbHLH119 322 35 894.47 5.91 45.22 -0.776 细胞核
L.us.o.m.scaffold63.33 LusbHLH120 283 30 547.05 9.34 44.75 -0.140 细胞核
L.us.o.m.scaffold63.99 LusbHLH121 222 25 080.61 5.61 74.16 -0.964 细胞核
L.us.o.m.scaffold65.109 LusbHLH122 132 14 434.28 8.79 46.15 -0.436 细胞核
L.us.o.m.scaffold67.164 LusbHLH123 589 64 555.00 5.50 51.14 -0.551 细胞核
L.us.o.m.scaffold67.61 LusbHLH124 1292 142 840.25 7.03 50.42 -0.341 细胞核
L.us.o.m.scaffold67.93 LusbHLH125 418 46 479.74 8.46 47.00 -0.137 细胞核
L.us.o.m.scaffold68.10 LusbHLH126 248 27 687.93 8.47 52.88 -0.585 细胞核
L.us.o.m.scaffold68.213 LusbHLH127 351 38 374.19 6.60 62.53 -0.378 细胞核
L.us.o.m.scaffold69.36 LusbHLH128 378 42 566.96 6.43 54.14 -0.647 细胞核
L.us.o.m.scaffold69.37 LusbHLH129 385 43 257.25 6.66 62.43 -0.701 细胞核
L.us.o.m.scaffold70.116 LusbHLH130 319 35 360.29 5.67 56.04 -0.799 细胞核
L.us.o.m.scaffold70.235 LusbHLH131 103 11 394.83 8.71 64.45 -0.524 细胞核
L.us.o.m.scaffold71.36 LusbHLH132 247 27 284.12 9.05 58.96 -0.341 细胞核
L.us.o.m.scaffold72.178 LusbHLH133 180 20 462.70 9.74 32.17 -0.393 细胞核
L.us.o.m.scaffold72.63 LusbHLH134 266 29 455.93 8.26 58.56 -0.603 细胞核
L.us.o.m.scaffold74.45 LusbHLH135 431 46 442.75 6.62 54.40 -0.635 叶绿体、细胞核
L.us.o.m.scaffold75.44 LusbHLH136 599 66 189.33 6.28 48.71 -0.496 细胞核
L.us.o.m.scaffold76.200 LusbHLH137 214 23 468.40 9.16 57.20 -0.417 细胞核
L.us.o.m.scaffold77.31 LusbHLH138 425 44 768.59 6.23 59.74 -0.445 细胞核
L.us.o.m.scaffold8.547 LusbHLH139 538 60 178.03 9.40 68.67 -0.410 细胞核
L.us.o.m.scaffold80.179 LusbHLH140 190 21 245.20 6.18 65.51 -0.342 细胞核
L.us.o.m.scaffold80.23 LusbHLH141 735 80 924.58 6.44 45.57 -0.369 细胞核
L.us.o.m.scaffold81.168 LusbHLH142 266 29 191.45 6.46 64.41 -0.763 细胞核
L.us.o.m.scaffold82.200 LusbHLH143 203 23 188.29 9.12 57.94 -0.577 细胞核
L.us.o.m.scaffold82.231 LusbHLH144 280 30 670.82 9.68 42.96 -0.881 细胞核
L.us.o.m.scaffold83.173 LusbHLH145 289 32 166.18 6.12 65.58 -0.842 细胞核
L.us.o.m.scaffold84.203 LusbHLH146 226 25 074.55 6.77 58.47 -0.661 细胞核
L.us.o.m.scaffold84.208 LusbHLH147 212 24 163.59 5.91 54.44 -0.513 细胞核
L.us.o.m.scaffold84.233 LusbHLH148 249 27 908.90 6.51 44.02 -0.401 细胞核
L.us.o.m.scaffold89.73 LusbHLH149 266 29 191.45 6.46 64.41 -0.763 细胞核
L.us.o.m.scaffold90.35 LusbHLH150 307 33 427.91 6.45 52.13 -0.624 细胞核
L.us.o.m.scaffold92.76 LusbHLH151 184 21 216.54 9.21 54.56 -0.518 细胞核
L.us.o.m.scaffold95.74 LusbHLH152 634 69 664.24 5.40 48.12 -0.705 细胞核
L.us.o.m.scaffold96.165 LusbHLH153 769 84 680.24 5.97 42.89 -0.373 细胞核
L.us.o.m.scaffold97.70 LusbHLH154 390 43 823.34 5.89 63.69 -0.543 细胞核
L.us.o.m.scaffold98.106 LusbHLH155 464 50 852.28 5.91 44.94 -0.386 液泡
L.us.o.m.scaffold98.129 LusbHLH156 290 31 160.43 5.82 46.99 -0.278 细胞核
L.us.o.m.scaffold98.173 LusbHLH157 274 29 832.75 7.66 56.56 -0.375 细胞核
L.us.o.m.scaffold98.174 LusbHLH158 1039 113 759.70 7.17 57.91 -0.602 细胞核
L.us.o.m.scaffold99.197 LusbHLH159 286 31 806.14 8.43 67.86 -0.298 细胞核

图1

亚麻bHLH家族基因的染色体定位

图2

亚麻bHLH家族蛋白系统发育进化树

图3

亚麻bHLH家族成员基因结构分析 (a) 进化树；(b) Motif预测；(c) 蛋白结构域；(d) 基因结构。

图4

亚麻bHLH家族成员启动子区域顺式作用元件分析 (a) bHLH家族启动子区域顺式作用元件；(b) 顺式作用元件热图。

图5

亚麻和拟南芥、陆地棉、毛果杨基因组之间bHLH基因共线性

图6

亚麻bHLH基因家族在亚麻茎中部不同发育时期的表达量分析

图7

qRT-PCR验证RNA-seq筛选的基因表达模式

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