Crops ›› 2017, Vol. 33 ›› Issue (1): 38-43.doi: 10.16035/j.issn.1001-7283.2017.01.007

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Mutagenic Effects of 60Co-γ-Ray Radiation on Dry Seeds of Foxtail Millet

Zhao Lijuan1,2,Ma Jinfeng2,Li Yandong2,Li Xiangyu2,Li Zhijiang2,Yuan Hongmei3,Guo Wendong4   

  1. 1Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,Heilongjiang,China
    2Crop Breeding Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
    3Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
    4Nature and Ecology Institute,Heilongjiang Academy of Sciences,Harbin 150040,Heilongjiang,China
  • Received:2016-10-13 Revised:2016-12-08 Online:2017-02-15 Published:2018-08-26

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Abstract:

In order to study the application of γ-ray in the mutation breeding of foxtail millet, four different doses of 60Co-γ ray were used to irradiate four different foxtail millet cultivars. A small amount of seeds after radiation were used for germination rate analysis. Seedling emergence date, seedling emergence rate, surviving plant rate, seedling ditstortion rate, etc of M1 generation were recorded, and the mutant individuals were screened in M2 generation. The results showed that there was no significant effect on germination rate in the dosage range of 0.20-0.35KGY, but the delayed emergence date extended, surviving plant rate decreased and seedling ditstortion rate increased with the increase of the dosage of irridiation. Radio sensitivity and suitable dosage of the four tested cultivars was significantly different. Radio sensitivity from strong to weak was: Chaogu 3-1, Longxuan 1, Longgu 32, Longgu 25. Suitable dosage of the varieties: Chaogu 3-1 for 0.25KGY, Longgu 32 for 0.25KGY, Longgu 25 for 0.30KGY, Longxuan 1 for 0.30KGY.

Key words: Foxtail millet, 60Co-γ ray, Radio sensitivity, Suitable radiation dosage

Table 1

Effects of 60Co-γ-ray radiation on seed germination and seedling emergence in M1 field"

品种（系）
Varieties (Strain)		 辐射剂量
(KGY)
Radiation dose		 发芽率(%)
Germination rate		 出苗期(d)
Seedling emergence day		 出苗率(%)Seedling emergence rate 成株率(%)
Survived rate		 畸变苗率(%)
Aberration rate		 辐射敏感性
Radio
sensitivity		 明显致矮剂量
Obvious dwarfing dose
龙谷32
Longgu 32		 0（CK） 94b 17 94.5b 100.0a 9.1e
0.20 93b 19 100.0a 90.0b 17.9d
0.25 85c 21 94.5b 73.3c 26.8c √ √
0.30 93b 27 73.0c 52.4d 56.4b √
0.35 97a 27 74.0c 28.1e 77.8a
龙谷25
Longgu 25		 0（CK） 93ab 17 91.5b 100.0a 7.5e
0.20 93ab 19 96.0a 85.0b 18.9d
0.25 92b 20 88.5c 74.5c 27.2c √ √
0.30 95a 27 73.5d 60.2d 39.4b √
0.35 81c 30 63.5e 57.8e 63.6a
朝谷3-1
Chaogu 3-1		 0（CK） 98a 19 71.0c 100.0a 13.5e
0.20 98a 21 83.5b 68.5b 19.0d √
0.25 85d 27 89.0a 52.3c 35.9c √
0.30 93b 30 58.0d 20.1d 40.7b
0.35 89c 32 58.0d 19.6d 67.8a
龙选1
Longxuan 1		 0（CK） 98a 19 90.0b 100.0a 2.3e
0.20 95b 20 93.5a 79.1b 14.6d
0.25 97ab 27 86.5c 65.2c 27.6c √
0.30 91c 27 83.8d 51.1d 34.1b √
0.35 99a 28 74.5e 17.6e 49.5a

Fig.1

Effect of different radiation dose on surviving plant rate of foxtail millet"

Table 2

The statistics of M2 generation mutants after 60Co-γ-ray radiation 株"

品种（系）
Varieties
(Strain)		 辐射剂量
(KGY)
Radiation dose		 早熟突变
Prematurity mutation		 晚熟突变
Late-maturing mutation		 矮化突变
Dwarf mutation		 穗型突变
Spike mutant		 高不育
High
infertility		 谷色突变
Color mutation of millet grains		 叶色突变
Leaf color mutation
朝谷3-1
Chaogu 3-1		 0.20 1 0 0 0 0 0 0
0.25 0 0 0 2 0 0 0
0.30 0 0 0 0 0 0 0
0.35 0 0 0 0 0 0 0
龙谷25
Longgu 25		 0.20 0 0 0 0 0 0 0
0.25 0 0 0 0 0 0 0
0.30 2 0 1 0 0 7 8
0.35 0 0 0 0 0 0 0
龙谷32
Longgu 32		 0.20 1 0 0 0 0 0 0
0.25 4 0 1 2 0 0 0
0.30 2 0 0 0 0 0 0
0.35 0 0 0 0 0 0 0
龙选1
Longxuan 1		 0.20 0 0 0 0 0 0 0
0.25 0 0 0 0 1 0 0
0.30 3 0 0 0 0 0 0
0.35 2 1 0 0 1 0 0
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