Crops ›› 2018, Vol. 34 ›› Issue (2): 68-72.doi: 10.16035/j.issn.1001-7283.2018.02.012

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Effects of Different Concentration Combinations of Hormone and Organics on Rapid Propagation of Sweet Potato Virus-Free Seedling

Yan Mingming,Chen Qiujun,Tang Min,Liu Zhiwen   

  1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China
  • Received:2017-12-08 Revised:2018-01-23 Online:2018-04-20 Published:2018-08-27

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Abstract:

The effects of the MS basic mediums adding different concentration of hormone or organics combination on the growth of virus-free sweet potato Ganshu No.1 seedling rapid propagation were studied in this research. The results indicated that the order of all hormones and organics affecting the sum of the indexes of the virus-free plantlet was as follow: 6-BA > NAA > IAA and niacin > inositol > VB1 > glycine > VB6. The optimal combination of hormones and organics were NAA (0mg/L)+6-BA (0.1mg/L)+IAA (0.1mg/L) and glycine (3.0mg/L)+VB1 (0.6mg/L)+VB6 (0.25mg/L)+niacin (100mg/L)+inositol (1.00mg/L). The total value of morphological indicators of the seedlings was 25.52, and the index of the rapid propagation was up to 24.98 after culturing under the optimal medium for 30 days.

Key words: Hormone, Organics, Sweet potato, Virus-free plantlet, Propagation

Table 1

Effects of the three hormones with different combination ratios to proliferation of sweet potato seedlings"

编号
Code		 NAA 6-BA IAA 空列
Null
column		 新生叶片数
No. of new leaf		 根生长数
No. of root		 茎尖新生数
No. of stem tip		 株高增加(cm)Increasing
plant height		 苗的增重量(g)
Increasing
seedling weight		 总值
Total value
Ⅰ1 1(1.0) 1(2.0) 1(2.0) 1 0.00±0.00g 1.33±0.15f 0.00±0.00g 0.00±0.00e 3.47±0.32ab 4.80
Ⅰ2 1 2(1.0) 2(1.0) 2 0.40±0.08f 4.60±0.98d 0.20±0.03f 0.00±0.00e 2.71±0.30d 7.91
Ⅰ3 1 3(0.5) 3(0.5) 3 0.80±0.08f 3.40±0.47e 2.00±0.19d 0.53±0.05cd 3.31±0.31bc 10.04
Ⅰ4 1 4(0.1) 4(0.1) 4 3.00±0.25cd 25.75±4.78a 2.75±0.26cd 0.77±0.07cd 3.34±0.24bc 35.61
Ⅰ5 1 5(0) 5(0) 5 3.40±0.32c 5.00±1.05d 5.00±0.85ab 1.98±0.16ab 2.90±0.27cd 18.28
Ⅰ6 2(0.5) 1 2 3 5.20±0.36b 3.40±0.51e 7.75±1.78a 0.78±0.08cd 3.89±0.41a 21.02
Ⅰ7 2 2 3 4 0.75±0.78f 2.75±0.32e 0.75±0.06e 0.65±0.06cd 3.06±0.26c 7.96
Ⅰ8 2 3 4 5 1.40±0.15e 2.80±0.42e 1.40±0.11de 0.30±0.03d 2.90±0.25cd 8.80
Ⅰ9 2 4 5 1 0.00±0.00g 1.40±0.13f 0.00±0.00g 0.50±0.06cd 2.89±0.28cd 4.79
Ⅰ10 2 5 1 2 2.60±0.19d 5.20±1.05d 2.60±0.23cd 1.06±0.78bc 2.90±0.26cd 14.36
Ⅰ11 3(0.1) 1 3 5 1.00±0.12ef 2.00±0.21f 1.40±0.11de 1.00±0.10bc 2.75±0.32d 8.15
Ⅰ12 3 2 4 1 1.00±0.11ef 12.6±2.78b 1.40±0.12de 0.60±0.06cd 3.40±0.29bc 19.00
Ⅰ13 3 3 5 2 0.40±0.07f 2.80±0.31e 1.20±0.08e 1.20±0.11bc 3.27±0.30bc 8.87
Ⅰ14 3 4 1 3 7.20±0.58a 2.00±0.18f 7.40±1.56a 2.64±0.25a 3.05±0.32c 22.29
Ⅰ15 3 5 2 4 3.80±0.34c 3.80±0.65de 4.00±0.56bc 1.14±0.12bc 3.76±0.38a 16.50
Ⅰ16 4(0.02) 1 4 2 0.80±0.09f 3.40±0.57e 1.40±0.13de 1.55±0.15b 3.21±0.28bc 10.36
Ⅰ17 4 2 5 3 2.40±0.22de 2.60±0.24e 3.00±0.32c 1.10±0.10bc 2.82±0.29cd 11.92
Ⅰ18 4 3 1 4 3.20±0.29cd 9.20±2.46bc 3.00±0.35c 2.04±0.17ab 3.93±0.45a 21.37
Ⅰ19 4 4 2 5 5.20±0.28b 1.80±0.15f 5.60±1.12a 2.10±0.18ab 2.98±0.33cd 17.68
Ⅰ20 4 5 3 1 6.00±0.51b 2.80±0.28e 5.20±0.97ab 2.66±0.28a 3.22±0.32bc 19.88
Ⅰ21 5(0) 1 5 4 1.60±0.17e 6.80±1.23c 1.40±0.15de 2.08±0.19ab 3.06±0.25c 14.94
Ⅰ22 5 2 1 5 6.00±0.45b 5.00±1.12d 6.60±1.26a 2.22±0.21a 3.38±0.25bc 23.20
Ⅰ23 5 3 2 1 2.60±0.21d 7.40±1.45c 2.40±0.20cd 2.50±0.18a 3.37±0.26bc 18.27
Ⅰ24 5 4 3 2 4.40±0.28c 7.00±1.27c 5.60±1.01a 2.30±0.25a 3.61±0.40ab 22.91
Ⅰ25 5 5 4 3 3.25±0.27cd 4.25±1.01d 3.50±0.48bc 0.86±0.09cd 3.06±0.31c 14.92
K1 76.64 59.27 86.02 66.74
K2 56.93 69.99 81.38 64.41
K3 74.81 67.35 68.94 80.19
K4 81.21 103.28 88.69 96.38
K5 94.24 83.94 58.80 76.11
R 7.46 8.80 5.98 6.39

Table 2

Effects of the five organics with different concentration combination to proliferation of sweet potato seedlings"

编号 Code 有机物Organic matter (mg/L) 结果Result
甘氨酸
Glycine		 VB1 VB6 肌醇
Inositol		 烟酸
Niacin		 新生叶片数
No. of new leaf		 根生长数
No. of root		 茎尖新生数
No. of stem tip		 株高增高(cm)
Increasing
plant height		 苗的增重量(g)
Increasing
seedling weight		 总值
Total
value
Ⅱ1 1(1.0) 1(0.2) 1(0.25) 1(50) 1(0.25) 2.00±0.26e 2.00±0.17b 3.00±0.29f 0.75±0.11d 4.63±0.51a 12.38
Ⅱ2 1 2(0.4) 2(0.50) 2(100) 2(0.50) 4.20±0.51cd 1.60±0.13b 4.60±0.42d 1.80±0.15b 4.06±0.37b 16.26
Ⅱ3 1 3(0.6) 3(0.75) 3(150) 3(0.75) 4.00±0.38cd 1.80±0.18b 4.40±0.39d 0.73±0.10d 4.51±0.48a 15.44
Ⅱ4 1 4(0.8) 4(1.00) 4(200) 4(1.00) 4.75±0.77bc 2.50±0.28a 6.00±0.72bc 2.13±0.22b 4.37±0.37ab 19.75
Ⅱ5 2(2.0) 1 2 3 4 4.20±0.49cd 2.00±0.18b 5.20±0.51c 1.60±0.17bc 3.75±0.34bc 16.75
Ⅱ6 2 2 1 4 3 4.20±0.59cd 2.00±0.22b 5.20±0.55c 1.90±0.21b 4.19±0.38ab 17.49
Ⅱ7 2 3 4 1 2 4.80±0.96bc 1.80±0.17b 5.80±0.48bc 1.34±0.15c 4.42±0.40a 18.16
Ⅱ8 2 4 3 2 1 5.80±1.29b 1.80±0.16b 6.60±0.68ab 3.24±0.41a 3.37±0.37c 20.81
Ⅱ9 3(3.0) 1 3 4 2 4.50±0.68c 2.50±0.22a 3.75±0.41e 1.58±0.14bc 4.37±0.32ab 16.70
Ⅱ10 3 2 4 3 1 5.20±1.16b 2.00±0.21b 5.00±0.47cd 2.68±0.35a 3.88±0.43bc 18.76
Ⅱ11 3 3 1 2 4 7.00±1.78a 2.00±0.16b 7.50±0.79a 2.50±0.28ab 4.19±0.48ab 23.19
Ⅱ12 3 4 2 1 3 3.40±0.41d 1.00±0.11c 5.20±0.45c 1.68±0.14bc 4.00±0.42b 15.28
Ⅱ13 4(4.0) 1 4 2 3 4.20±0.47cd 1.80±0.16b 4.20±0.41de 1.70±0.16bc 4.32±0.44ab 16.22
Ⅱ14 4 2 3 1 4 4.80±0.87bc 1.60±0.14b 5.20±0.49c 1.84±0.17b 4.46±0.51a 17.90
Ⅱ15 4 3 2 4 1 4.40±0.58c 2.00±0.19b 4.20±0.44de 1.66±0.15bc 4.27±0.39ab 16.53
Ⅱ16 4 4 1 3 2 2.60±0.32e 0.80±0.09c 3.60±0.40ef 1.16±0.12cd 4.36±0.41ab 12.52
K1 63.83 62.05 65.58 63.72 68.48
K2 73.21 70.41 64.82 76.48 63.64
K3 73.93 73.32 70.85 63.47 64.43
K4 63.17 68.36 72.89 70.47 77.59
R 2.69 2.82 2.02 3.25 3.49

Table 3

The comparison of the growing performance of the virus-free plantlet on the un-optimization and optimization mediums"

处理
Treatment		 新生叶片数
No. of new leaf		 根生长数
No. of root		 茎尖新生数
No. of stem tip		 株高增高(cm)
Increasing plant height		 苗的增重量(g)
Increasing
seedling weight		 总值
Total
value		 繁殖系数
Propagation
coefficient
MS 5.00±0.45c 2.50±0.36a 5.05±0.29b 2.45±0.21b 2.95±0.36b 18.40 11.21±1.34c
MS+NAA(0mg/L)+6-BA
(0.1mg/L)+IAA(0.1mg/L)		 6.70±0.68b 2.31±0.21a 7.46±1.27a 2.84±0.25a 4.05±0.44a 23.36 20.36±1.81b
MS+Ⅱ11 8.41±1.25a 2.45±0.26a 7.09±0.59a 3.12±0.28a 4.45±0.41a 25.52 24.98±2.51a
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