Crops ›› 2017, Vol. 33 ›› Issue (4): 117-122.doi: 10.16035/j.issn.1001-7283.2017.04.020

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Effects of Exogenous ABA and Its Inhibitor Fluridone on Cold Resistance Indicators of Winter Wheat Tiller Node

Yang Ning1,Zhao Botong2,Bao Yuzhuo1,Lü Yan1,Peng Kankan1,Tian Yu1,Wang Junhong1,Meng Jing1,Cang Jing1   

  1. 1 College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
    2 Guangdong Baiyun University,Guangzhou 510450,Guangdong,China
  • Received:2017-04-13 Revised:2017-06-19 Online:2017-08-15 Published:2018-08-26
  • Contact: Jing Cang

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Abstract:

To investigate the effects of exogenous abscisic acid (ABA) on the cold-resistance of winter wheat, an elite freeze-tolerant cultivar ‘Dongnongdongmai No.1’ was selected as material and 10μmol/L ABA and 50μmol/L ABA inhibitor fluridone were sprayed (water treatment as the control) at the trefoil stage. The tiller nodes were collected for measuring physiological indicators when the ambient temperature reached approximately at 4℃, 0℃, -10℃ and -25℃. The results showed that with the decreasing of temperature, the content of soluble protein and soluble sugar presented upgrade firstly then descending latter. The content of proline and malondialdehyde (MDA), and activity of superoxide dismutase (SOD) increased progressively. The content of soluble sugar, soluble protein and proline, and SOD activity under the ABA treatment were significantly higher than that under the control, but the content of MDA was lower than that under ABA treatment. The indicators under fluridone treatment showed reverse trend with ABA treatment, indicating that exogenous ABA could increase the content of ABA in plant, then increase the content of osmolytes, finally improve the activity of antioxidant enzymes to increase the cold-resistance of Dongnongdongmai No.1. Therefore, a certain concentration of fluridone could be regarded as an ABA inhibitor to study the physiology of plant stress.

Key words: Dongnongdongmai No.1, Abscisic acid, Fluridone, Physiological index, Cold-resistance

Fig.1

Sampling date of Dongnongdongmai No.1 at different temperatures"

Fig.2

The effects of sampling temperature on soluble sugar content of tiller node in winter wheat Different letters mean significant difference among treatments (P < 0.05), the same below"

Fig.3

The effects of sampling temperature on soluble protein content of tiller node in winter wheat"

Fig.4

The effects of sampling temperature on proline content of tiller node in winter wheat"

Fig.5

The effects of sampling temperature on MDA content of tiller node in winter wheat"

Fig.6

The effects of sampling temperature on SOD activity of tiller node in winter wheat"

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