Crops ›› 2017, Vol. 33 ›› Issue (4): 117-122.doi: 10.16035/j.issn.1001-7283.2017.04.020

Previous Articles     Next Articles

Effects of Exogenous ABA and Its Inhibitor Fluridone on Cold Resistance Indicators of Winter Wheat Tiller Node

Yang Ning1,Zhao Botong2,Bao Yuzhuo1,Lü Yan1,Peng Kankan1,Tian Yu1,Wang Junhong1,Meng Jing1,Cang Jing1   

  1. 1 College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
    2 Guangdong Baiyun University,Guangzhou 510450,Guangdong,China
  • Received:2017-04-13 Revised:2017-06-19 Online:2017-08-15 Published:2018-08-26
  • Contact: Jing Cang

RichHTML

8

PDF (PC)

247

Abstract:

To investigate the effects of exogenous abscisic acid (ABA) on the cold-resistance of winter wheat, an elite freeze-tolerant cultivar ‘Dongnongdongmai No.1’ was selected as material and 10μmol/L ABA and 50μmol/L ABA inhibitor fluridone were sprayed (water treatment as the control) at the trefoil stage. The tiller nodes were collected for measuring physiological indicators when the ambient temperature reached approximately at 4℃, 0℃, -10℃ and -25℃. The results showed that with the decreasing of temperature, the content of soluble protein and soluble sugar presented upgrade firstly then descending latter. The content of proline and malondialdehyde (MDA), and activity of superoxide dismutase (SOD) increased progressively. The content of soluble sugar, soluble protein and proline, and SOD activity under the ABA treatment were significantly higher than that under the control, but the content of MDA was lower than that under ABA treatment. The indicators under fluridone treatment showed reverse trend with ABA treatment, indicating that exogenous ABA could increase the content of ABA in plant, then increase the content of osmolytes, finally improve the activity of antioxidant enzymes to increase the cold-resistance of Dongnongdongmai No.1. Therefore, a certain concentration of fluridone could be regarded as an ABA inhibitor to study the physiology of plant stress.

Key words: Dongnongdongmai No.1, Abscisic acid, Fluridone, Physiological index, Cold-resistance

Fig.1

Sampling date of Dongnongdongmai No.1 at different temperatures"

Fig.2

The effects of sampling temperature on soluble sugar content of tiller node in winter wheat Different letters mean significant difference among treatments (P < 0.05), the same below"

Fig.3

The effects of sampling temperature on soluble protein content of tiller node in winter wheat"

Fig.4

The effects of sampling temperature on proline content of tiller node in winter wheat"

Fig.5

The effects of sampling temperature on MDA content of tiller node in winter wheat"

Fig.6

The effects of sampling temperature on SOD activity of tiller node in winter wheat"

[1] 于晶, 张林, 崔红 , 等. 高寒地区冬小麦东农冬麦1号越冬前的生理生化特性. 作物学报, 2008,34(11):2019-2025.
doi: 10.3724/SP.J.1006.2008.02019
[2] Ton J, Flors V, Mauch-Mani B . The multifaceted role of ABA in disease resistance. Trends in Plant Science, 2009,14(6):310-317.
doi: 10.1016/j.tplants.2009.03.006 pmid: 19443266
[3] De S I, Zhang H , Inze D.et,al.A novel role for abscisic acid emerges from underground. Trends in Plant Science, 2006,11(9):434-439.
doi: 10.1016/j.tplants.2006.07.003 pmid: 16890475
[4] Chinnusamy V, Gong Z, Zhu J K . Abscisic acid-mediated epigenetic processes in plant development and stress responses. Journal of Integrative Plant Biology, 2008,50(10):1187-1195.
doi: 10.1111/j.1744-7909.2008.00727.x pmid: 19017106
[5] 田士林, 李莉 . 低温处理对小麦叶片中 ABA 含量的影响. 湖北农业科学, 2007,46(5):697-698.
doi: 10.3969/j.issn.0439-8114.2007.05.015
[6] 刘丽杰, 苍晶, 李怀伟 , 等. 外源ABA对冬小麦越冬期呼吸代谢关键酶与糖代谢的影响. 麦类作物学报, 2013,33(1):65-72.
doi: 10.7606/j.issn.1009-1041.2013.01.012
[7] 刘春玲, 陈慧萍, 刘娥娥 , 等. 水稻品种对几种逆境的多重耐性及与ABA的关系. 作物学报, 2003,29(5):725-729.
[8] 于晶, 张林, 苍晶 , 等. 外源ABA对寒地冬小麦东农冬麦1号幼苗生长及抗冷性的影响. 麦类作物学报, 2008,28(5):883-867.
doi: 10.7606/j.issn.1009-1041.2008.05.188
[9] Anderson M D, Prasad T K, Martin B A , et al. Differential gene expression in chilling-acclimated maize seedlings and evidence for the involvement of abscisic acid in chilling tolerance. Plant Physiology, 1994,105(1):331-338.
doi: 10.1104/pp.105.1.331
[10] 黄杏, 陈明辉, 杨丽涛 , 等. 低温胁迫下外源ABA对甘蔗幼苗抗寒性及内源激素的影响. 华中农业大学学报, 2013,34(4):6-11.
[11] Yoshioka T, Endo T, Satoh S . Restoration of seed germination at supraoptimal temperatures by fluridone,an inhibitor of abscisic acid biosynthesis. Plant Cell Physiology, 1998,39(3):307-312.
doi: 10.1093/oxfordjournals.pcp.a029371
[12] Rasmussen R D, Hole D, Hess J R , et al. Wheat kernel dormancy and (+)-abscisic acid level following exposure to fluridone. Journal of Plant Physiology, 1997,150(4):440-445.
doi: 10.1016/S0176-1617(97)80095-8
[13] 郝再彬, 苍晶, 徐仲 , 等. 植物生理实验.哈尔滨: 哈尔滨工业大学出版社, 2004.
[14] Liu J, Jiang M Y, Zhou Y F , et al. Production of polyamines is enhanced by endogenous abscisic acid in maize seedlings subjected to salt stress. Journal of Integrative Plant Biology, 2005,47(11):1326-1334.
doi: 10.1111/jipb.2005.47.issue-11
[15] Iqbal M, Ashraf M , Shafiq-ur-Rehman,et al.Does polyamine seed pretreatment modulate growth and levels of some plant growth regulators in hexaploid wheat (Triticum aestivum L.) plants under salt stress?. Botanical Studies, 2006,47(3):239-250.
[16] 陈尚武, 张大鹏 . ABA和Fluridone对苹果果实成熟的影响. 植物生理学报, 2000,26(2):123-129.
[17] 李馨园, 杨晔, 张丽芳 , 等. 外源ABA对低温胁迫下玉米幼苗内源激素含量及Asr1基因表达的调节. 作物学报, 2017,43(1):141-148.
[18] Galiba G, Kerepesi I . Mapping of genes involved in glutathione,carbohydrate and CORl4b cold induced protein accumulation during cold hardening in wheat. Euphytica, 2001,119:173-177.
doi: 10.1023/A:1017532725385
[19] 宋永骏, 杨延杰 . 亚低温对茄子幼苗叶片渗透调节物质含量及活性氧清除物质的影响. 华北农学报, 2011,26(4):228-231.
doi: 10.7668/hbnxb.2011.04.041
[20] Trovato M, Mattioli R, Costantino P . Multiple roles of proline in plant stress tolerance and development. Rendiconti Lincei, 2008,19(4):325-346.
doi: 10.1007/s12210-008-0022-8
[21] Savouré A . Proline:a multifunctional amino acid. Trends in Plant Science, 2010,15(2):89-97.
doi: 10.1016/j.tplants.2009.11.009 pmid: 20036181
[22] 董绪兵, 毕焕改, 刘业霞 , 等. 黄瓜幼苗干旱-低温交叉适应与渗透调节的关系. 中国农业科学, 2011,44(2):335-340.
doi: 10.3864/j.issn.0578-1752.2011.02.013
[23] 邓凤飞, 杨双龙, 龚明 . 外源ABA对低温胁迫下小桐子幼苗脯氨酸积累及其代谢途径的影响. 植物生理学报, 2015,51(2):221-226.
[24] 王秀田, 卢秋巍, 苍晶 , 等. 低温驯化对冬小麦叶绿素荧光特性及抗氧化酶活性的影响. 植物生理学报, 2016,52(12):1959-1969.
[25] 宋广树, 孙忠富, 孙蕾 , 等. 东北中部地区水稻不同生育时期低温处理下生理变化及耐冷性比较. 生态学报, 2011,31(13):3788-3795.
[26] 何开跃, 李晓储, 黄利斌 , 等. 冷冻胁迫对福建柏苗可溶性糖和丙二醛(MDA)含量的影响. 江苏林业科技, 2007,27(6):6-8.
[27] 李晶, 阎秀峰, 祖元刚 . 低温胁迫下红松幼苗活性氧的产生及保护酶的变化. 植物学报, 2000,42(2):148-152.
[28] 王宇, 王晶英 . 脱落酸对低温胁迫下水曲柳幼苗叶片抗寒生理指标的影响. 森林工程, 2010,26(4):32-36.
[1] Jingwen Fang,Yan Wu,Zhihua Liu. Effects of Salt Stress on Seed Germination and Physiological Characteristics of Apocynum venetum [J]. Crops, 2018, 34(4): 167-174.
[2] Xiaoyong Zhang,Youlian Yang,Shujiang Li,Rongchuan Xiong,Hong Xiang. Effects of Exogenous GA3 and 6-BA on Leaf Senescence in Low Temperature Stress of Virus-Free Potato Cutting Seedlings [J]. Crops, 2018, 34(4): 95-101.
[3] Xiyu Hao,Hongdan Wang,Zhichao Yin,Jie Liang,Fengxiang Yin,Jianjun Hao. Effects of PEG Stress on Drought Resistance at Seedling Stage of Adzuki Beans and the Establishment of Drought Resistance Identification System [J]. Crops, 2017, 33(4): 134-142.
[4] ,Lili Zhang,Ying Shi. Screening of Drought Resistant Germplasm Resources in Potato [J]. Crops, 2017, 33(4): 72-77.
[5] Xiaoxiao Xu,Jianqiang Zhu,Qixia Wu. Alleviation of Abscisic Acid and Nutrient to Waterlogged Wheat at Booting and Filling Stage [J]. Crops, 2016, 32(6): 85-90.
[6] Cuicui Yang,Fade Li,Yan Li,Chenglai Wu,Chunqing Zhang. Effects of Arc-Tooth-Shaped Corona Discharge Field Treatment on Improving Corn Seed Vigor and Physiological Indexes [J]. Crops, 2016, 32(6): 154-159.
[7] Yingmei Zuo,Weize Yang,Tianmei Yang,Meiquan Yang,Zongliang Xu,Shaobing Yang,Jinyu Zhang. Comparison of Resistant Physiological Index among Four Species in the Genus Panax under Water Stress [J]. Crops, 2016, 32(3): 84-88.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .