Crops ›› 2019, Vol. 35 ›› Issue (5): 64-68.doi: 10.16035/j.issn.1001-7283.2019.05.011

Previous Articles     Next Articles

Screening of Chemical Male Killing Agents for Millet

Guo Ruifeng,Ren Yuemei,Yang Zhong,Ren Guangbing,Zhang Shou,Feng Jing   

  1. Institute of Crops in High Latitude and Cold Climate Area, Shanxi Academy of Agricultural Sciences, Datong 037008, Shanxi, China
  • Received:2019-05-20 Revised:2019-08-12 Online:2019-10-15 Published:2019-11-07
  • Contact: Yuemei Ren

RichHTML

5

PDF (PC)

186

Abstract:

In order to enrich the cross-breeding methods of millet and improve the efficiency of cross-breeding, five kinds of chemical reagents were used to kill the male of different varieties of millet at the primordium differentiation stage of pistil and stamen of millet. Based on the fruiting of the treated millet after self-cross and saturated pollination, glyphosate ammonium salt was selected as the suitable chemical male killing agent for millet, and the suitable concentration of glyphosate ammonium salt was further screened. The results showed that the optimum application concentration of Datong 34 and Datong 27 were 3.75-5.00mg/L, and that of Dabaigu was 10.00mg/L. The best application concentration was different when the varieties were different.

Key words: Millet, Chemical male killing agent, Screening

Table 1

Chemical reagents and working concentrations"

化学杀雄剂
Chemical male killing agent		 施用浓度(mg/L)
Working concentration
草甘膦铵盐(75.7%) Glyphosate ammonium salt 2.5 5.0 10.0
乙烯利(40%) Ethrel 2 500 5 000 10 000
单嘧磺隆(10%) Monosulfuron 0.25 0.50 1.00
咪唑乙烟酸(5%) Imidazolinic acid 2.5 5.0 10.0
赤霉素(90%) Gibberellin 100 200 400

Table 2

Effects of different chemical reagent treatments on relative self-cross sterility rates of Datong 27 and Datong 34 %"

处理
Treatment		 试剂
Reagent		 浓度(mg/L)
Concentration		 大同27号Datong 27 大同34号Datong 34
Ⅰ Ⅱ Ⅲ 均值Mean value Ⅰ Ⅱ Ⅲ 均值Mean value
1 草甘膦铵盐 2.5 41.47 44.06 41.36 42.30Gg 25.14 25.86 23.73 24.91Hi
2 Glyphosate ammonium salt 5.0 69.89 69.02 71.11 70.01Dd 70.38 71.10 69.80 70.43CDcd
3 10.0 93.30 93.46 92.37 93.04Bb 98.50 98.14 98.50 98.38Aa
4 乙烯利Ethrel 2 500 44.03 44.09 46.78 44.97Ff 44.18 33.31 39.54 39.01FG
5 5 000 69.90 70.84 68.48 69.74Dd 30.52 29.65 21.85 27.34Hi
6 10 000 84.16 83.83 84.48 84.16Cc 69.84 68.14 62.03 66.67Dd
7 单嘧磺隆Monosulfuron 0.25 24.86 24.18 27.51 25.52Ii 42.03 42.37 46.93 43.78Ff
8 0.50 38.62 38.62 37.93 38.39Hh 32.69 33.49 32.71 32.96Gh
9 1.00 59.35 58.39 60.00 59.25Ee 74.36 73.27 74.52 74.05BCbc
10 咪唑乙烟酸 2.5 83.76 84.57 83.71 84.01Cc 76.58 74.63 74.66 75.29BCb
11 Imidazolinic acid 5.0 84.01 84.22 83.76 83.99Cc 77.54 77.74 78.19 77.82Bb
12 10.0 99.56 99.45 99.37 99.46Aa 99.91 99.81 99.66 99.80Aa
13 赤霉素Gibberellin 100 68.65 69.54 68.85 69.01Dd 68.07 68.19 67.92 68.06Dd
14 200 43.51 41.75 43.65 42.97FGg 52.92 53.91 55.90 54.24Ee
15 400 99.93 99.91 99.87 99.90Aa 67.20 68.01 67.18 67.46Dd

Table 3

Effects of different chemical reagents on the relative seed setting rate after saturated pollination of Datong 27 and Datong 34 %"

处理
Treatment		 试剂
Reagent		 浓度
Concentration
(mg/L)		 大同27号Datong 27 大同34号Datong 34
饱和授粉相对不育率
Relative sterility rate after
saturated pollination		 饱和授粉相对结实率
Relative seed setting rate after saturated pollination		 饱和授粉相对不育率
Relative sterility rate after
saturated pollination		 饱和授粉相对结实率
Relative seed setting rate after saturated pollination
Ⅰ Ⅱ Ⅲ 均值
Mean value		 Ⅰ Ⅱ Ⅲ 均值
Mean value
1 草甘膦铵盐 2.5 1.17 4.76 0.63 2.19 97.81 23.70 24.83 23.56 24.03 75.97
2 Glyphosate 5.0 35.71 34.47 37.02 35.73 64.27 60.84 60.99 61.88 61.24 38.76
3 ammonium salt 10.0 93.14 93.27 92.11 92.84 7.16 90.64 89.86 91.14 90.54 9.46
4 乙烯利Ethrel 2 500 46.93 43.63 44.97 45.18 54.82 44.04 33.22 37.35 38.20 61.80
5 5 000 53.61 55.85 52.88 54.11 45.89 30.05 27.77 22.33 26.72 73.28
6 10 000 85.64 82.79 84.46 84.30 15.70 69.61 67.90 65.64 67.72 32.28
7 单嘧磺隆 0.25 17.37 17.16 25.31 19.94 80.06 41.11 41.93 45.37 42.80 57.20
8 Monosulfuron 0.50 29.30 27.37 29.70 28.79 71.21 29.60 32.68 30.45 30.91 69.09
9 1.00 58.50 55.32 60.12 57.98 42.02 67.38 65.19 67.56 66.71 33.29
10 咪唑乙烟酸 2.5 83.54 84.16 83.31 83.67 16.33 75.71 74.23 74.32 74.75 25.25
11 Imidazolinic acid 5.0 83.72 84.07 83.47 83.75 16.25 77.43 77.57 78.15 77.71 22.29
12 10.0 99.50 99.48 99.28 99.42 0.58 99.90 99.85 99.62 99.79 0.21
13 赤霉素 100 70.32 69.48 67.17 68.99 31.01 57.22 57.93 56.92 57.36 42.64
14 Gibberellin 200 38.11 36.63 38.39 37.71 62.29 53.38 53.73 54.79 53.97 46.03
15 400 98.02 97.83 97.91 97.92 2.08 63.96 66.85 68.26 66.36 33.64

Fig.1

Effects of different treatments on self-cross in bagging and saturated pollination relative seed setting rate of Datong 27"

Fig.2

Effects of different treatments on self-cross in bagging and saturated pollination relative seed setting rate of Datong 34"

Table 4

Effects of different spraying concentrations of glyphosate ammonium salt on relative sterility rate of different millet varieties %"

品种
Variety		 浓度(mg/L)
Concentration		 自交相对不育率
Relative self-cross
sterility rate		 饱和授粉后相对不育率
Relative sterility rate after
saturated pollination		 饱和授粉后提高结实率
Increasing seed setting rate
after saturated pollination
大同27号Datong 27 2.50 21.80bA 21.71bA 0.08
3.75 31.27bA 24.68bA 6.60
5.00 28.15bA 25.42bA 2.73
7.50 44.81abA 45.00abA -0.19
10.00 70.92aA 70.89aA 0.03
大同34号Datong 34 2.50 8.01cC 6.95bB 1.06
3.75 22.65bcB 19.98bAB 2.67
5.00 48.05abAB 41.95abA 6.11
7.50 63.78aA 63.65aA 0.14
10.00 68.18aA 69.93aA -1.75
大白谷Dabaigu 2.50 20.80cB 20.51cC 0.28
3.75 31.27cB 25.68cC 5.60
5.00 41.04bcB 38.30bcBC 2.73
7.50 57.70abAB 57.89abAB -0.19
10.00 81.09aA 73.71aA 7.38
[1] 智慧, 王永强, 李伟 , 等. 利用野生青狗尾草的细胞质培育谷子质核互作雄性不育材料. 植物遗传资源学报, 2007,8(3):261-264.
[2] 赵治海, 崔文生 . 谷子光(温)敏不育系821选育及其不育性与光、温关系的研究. 中国农业科学, 1996,29(5):23-31.
[3] 王玉文, 王随保, 李会霞 , 等. 谷子光敏雄性不育系选育及应用研究. 中国农业科学, 2003,36(6):714-717.
[4] 王春芳, 张温典, 刘国庆 , 等. 谷子不育系的应用与杂种优势研究进展. 河北民族师范学院学报, 2017,37(1):116-122.
[5] 辛淑芳 . 谷子杂交技术—温汤去雄接触授粉法. 内蒙古农业科技, 1973(6):13-16.
[6] 史宏, 陈瑛, 史关燕 , 等. 谷子太阳能杀雄技术的研究. 杂粮作物, 2008,28(6):360-362.
[7] Moore R H . Several effects of maleic hydrazide on plants. Science, 1950,112:52-53.
[8] Naylor A W . Observations on the effects of maleic hydrazide on the flowering of tobacco,maize and cocklebur. Proceedings of the National Academy of Sciences of the United States of America, 1950,36(4):230-232.
[9] 刘宏伟, 张改生, 王军卫 , 等. 化学杂交剂SQ-1诱导小麦雄性不育及与不同小麦品种互作效应的研究. 西北农林科技大学学报(自然科学版), 2003,31(4):15-18.
[10] 官春云, 王国槐, 赵均田 , 等. “杀雄剂一号”诱导油菜雄性不育的效果及其原理的研究. 遗传, 1981,3(5):15-17.
[11] 官春云, 王国槐 . 几种化学药物对油菜杀雄效果的研究. 作物研究, 1993,7(3):13-16.
[12] 崔秀辉 . 化学杂交剂SQ-1诱导糜子雄性不育效果研究. 作物学报, 2008,34(1):106-110.
doi: 10.3724/SP.J.1006.2008.00106
[13] 张小东, 冯佰利, 王鹏科 , 等. 糜子化学杀雄剂筛选及杀雄效果研究初报. 西北农业学报, 2011,20(8):77-81.
[14] 侯国裕, 黄景航, 王增骐 , 等. 甲基胂酸钠作为水稻化学杀雄剂的研究. 化学通讯, 1979,4(2):15-21.
[15] 黄雪清, 高东迎, 杨安南 , 等. 化学杀雄剂Ⅲ号诱导水稻雄性不育过程中幼穗、颖花、花药中核酸和蛋白质代谢研究. 作物学报, 2001,27(6):827-831.
[16] 史关燕, 史更生, 史宏 , 等. 谷子化学杀雄技术的探讨. 杂粮作物, 2001,21(6):29-30.
[1] Shi Liran,Hao Hongbo,Cui Haiying,Li Mingzhe. Effects of Shading on Photosynthetic Characteristics and Rapid Chlorophyll Fluorescence Kinetic Characteristics of Foxtail Millet [J]. Crops, 2019, 35(5): 125-128.
[2] Zhang Yongfang,Qian Xiaona,Wang Runmei,Shi Pengqing,Yang Rong. Identification of Drought Resistance of Different Soybean Materials and Screening of Drought Tolerant Varieties [J]. Crops, 2019, 35(5): 41-45.
[3] Yue Linqi,Shi Weiping,Guo Jiahui,Guo Pingyi,Guo Jie. Response of Cutin Synthetic Genes of Foxtail Millet to Drought Stress [J]. Crops, 2019, 35(4): 183-190.
[4] Zhang Yaowen,Hou Junli,Zhao Xiaoguang,Guan Zhoubo,Li Dianrong,Tian Jianhua,Dong Yuhong,Wang Zhuyun. Comprehensive Identification and Selection System and Method for Rape Germplasm with High Photosynthetic Efficiency [J]. Crops, 2019, 35(4): 69-76.
[5] Li Yanfang,Du Yanwei,Zhang Zheng,Wang Gaohong,Zhao Genyou,Zhao Jinfeng,Yu Aili. Establishment and Optimization of Agrobacterium Mediated Transformation System for Mature Embryo of Foxtail Millet [J]. Crops, 2019, 35(3): 73-79.
[6] Shaoguang Liu,Xiatong Zhao,Xi’e Song,Xiangyang Yuan,Shuqi Dong,Meijun Guo,Pingyi Guo. Effects of Intermembrane Spraying Pre-Germination Herbicide on the Safety of Millet and Weed Control [J]. Crops, 2019, 35(2): 173-178.
[7] Zhiyong Hao,Guangdong Yang,Guangwei Qiu,Zunyan Hu,Lichun Wang,Haiyan Wang. Screening of High Carotenoid Resources in Potato [J]. Crops, 2019, 35(2): 71-77.
[8] Ying Fu,Yinan Shen,Yanchun Liu,Xiaojiao Chai,Xianrui Wang,Xiaolei Bai,Shutian Li. Correlation Analysis of Amylopectin Content, Nutritional Quality and Agronomic Traits in Spring Millet Varieties [J]. Crops, 2019, 35(2): 90-93.
[9] Yanwei Du,Jinfeng Zhao,Gaohong Wang,Yanfang Li,Genyou Zhao,Xiaoguang Yan. Study of Lodging Resistance of Spring-Sowing Foxtail Millet in Maturity Stages [J]. Crops, 2019, 35(1): 141-145.
[10] Si Chen,Xue Yang,Xiukun Yang,Hongmei Yuan,Wengong Huang,Yan Liu,Yubo Yao,Guangwen Wu. Screening Pasmo-Resistant Germplasm Resources from Flax Varieties (Lines) [J]. Crops, 2019, 35(1): 63-67.
[11] Xinling Yang,Qian Yao,Wenli Ping,Yiqiong Ma,Baolin Wang,Guotao Jia,Yongfeng Yang,Hong Cui. Screening of High Aroma Mutants from Progenies of EMS Mutagenized Flue-Cured Tobacco [J]. Crops, 2019, 35(1): 68-74.
[12] Xiaolan Jing,Zhihua Li,Xun Dong. Effects of Different Sowing Dates on Growth and Yield of Different Millet Varieties [J]. Crops, 2019, 35(1): 146-151.
[13] Ruiyu Ren,Jihong He,Kongjun Dong,Lei Zhang,Tianpeng Liu,Tianyu Yang. Analysis on Suitable Sowing Date of Four Main Cultivars of Broomcorn Millet in Huining, Gansu [J]. Crops, 2019, 35(1): 152-158.
[14] Li Chunhong,Lu Xianglong,Zhang Peitong,Su Yanjing,Wang Yiming,Guo Wenqi,Yin Jianmei,Han Xiaoyong,Wang Li,Huo Enjie. Screening Herbicides to Control Weeds for Sweet Sorghum [J]. Crops, 2018, 34(6): 158-161.
[15] Meiri· Aheihati,Wumaierxiati· Tahan,Tahan· Ailinbieke,Wang Yuxiang. Phenotypic Diversity of Proso Millet (Panicum miliaceum L.) Germplasm Resources in Xinjiang and Its Surrounding Areas [J]. Crops, 2018, 34(6): 48-52.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wang Haitao,Liu Cunjing,Tang Liyuan,Zhang Sujun,Li Xinghe,Cai Xiao,Zhang Xiangyun,Zhang Jianhong. Status and Developmental Tendency of Hybrid Cotton in Hebei Province[J]. Crops, 2019, 35(5): 1 -8 .
[2] Liu Nianxi,Chen Liang,Li Zhi,Liu Baoquan,Liu Jia,Yi Zhigang,Dong Zhimin,Wang Shuming. Advances in Molecular Markers of Soybean Disease Resistance[J]. Crops, 2019, 35(4): 10 -16 .
[3] Huang Yufang,Ye Youliang,Zhao Yanan,Yue Songhua,Bai Hongbo,Wang Yang. Effects of Nitrogen Application Rates on Yield and Mineral Concentrations of Winter Wheat Grains in the North of Henan Province[J]. Crops, 2019, 35(5): 104 -108 .
[4] Meng Fanlai,Guo Huachun. Effects of Enhanced UV-B on Photosynthetic Characteristics and UV-Absorbing Compounds of Sweet Potato[J]. Crops, 2019, 35(5): 114 -119 .
[5] Zhang Yanhua,Chang Xuhong,Wang Demei,Tao Zhiqiang,Wang Yanjie,Yang Yushuang,Zhao Guangcai. Effects of Zinc Topdressing Fertilizer on Yield and Quality of Wheat under Different Soil Conditions[J]. Crops, 2019, 35(5): 109 -113 .
[6] Li Song,Zhang Shicheng,Dong Yunwu,Shi Delin,Shi Yundong. Genetic Diversity Analysis of Rice Varieties in Tengchong, Yunnan Based on SSR Markers[J]. Crops, 2019, 35(5): 15 -21 .
[7] Wang Yongxing,Shan Feibiao,Yan Wenzhi,Du Ruixia,Yang Qinfang,Liu Chunhui,Bai Lihua. Genetic Diversity Analysis and Code Classification Based on DUS Testing in Sunflower[J]. Crops, 2019, 35(5): 22 -27 .
[8] Shi Zhaokang,Zhao Zequn,Zhang Yuanhang,Xu Shiying,Wang Ning,Wang Weijie,Cheng Hao,Xing Guofang,Feng Wanjun. The Response and Cluster Analysis of Biomass Accumulation and Root Morphology of Maize Inbred Lines Seedlings to Two Nitrogen Application Levels[J]. Crops, 2019, 35(5): 28 -36 .
[9] Zhang Zhongwei,Yang Hailong,Fu Jun,Xie Wenjin,Feng Guang. Genetic Analysis of the Kernel Length of Maize with Mixed Model of Major Gene Plus Polygene[J]. Crops, 2019, 35(5): 37 -40 .
[10] Zhang Yongfang,Qian Xiaona,Wang Runmei,Shi Pengqing,Yang Rong. Identification of Drought Resistance of Different Soybean Materials and Screening of Drought Tolerant Varieties[J]. Crops, 2019, 35(5): 41 -45 .