Crops ›› 2022, Vol. 38 ›› Issue (4): 167-171.doi: 10.16035/j.issn.1001-7283.2022.04.023

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Response of Tobacco Seedling Growth and Development to Combined Application of Brassinolide and Microbial Agents

Li Diqin1(), Peng Yuanyuan1, Wang Yan1, Li Sijun2, Peng Tianwei1, Wang Qing1, Li Qiang1, Xie Huiya3()   

  1. 1College of Agronomy, Hunan Agricultural University, Changsha 410128, Hunan, China
    2Chenzhou Company of Hunan Tobacco Company, Chenzhou 423000, Hunan, China
    3Zhuzhou Company of Hunan Tobacco Company, Zhuzhou 412000, Hunan, China
  • Received:2021-04-12 Revised:2022-06-14 Online:2022-08-15 Published:2022-08-22
  • Contact: Xie Huiya E-mail:ldqhnnd2009@163.com;290615790@qq.com

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Abstract:

In order to study the effects of brassinolide and Bacillus subtilis on the growth and development of tobacco seedlings, Xiangyan 5 was used as the material, and different concentrations of brassinolide and Bacillus subtilis agents were sprayed on tobacco seedlings of four leaves-one core. The amount of dry matter accumulation, root activity, chlorophyll levels, and related enzyme activities of eight leaves-one core of tobacco seedlings were measured and analysed. The results demonstrated that the amount of dry matter accumulation, root activity, chlorophyll content, and nitrate reductase activity of tobacco seedlings treated with brassinolide and Bacillus subtilis agents were superior to those of the controls; and the treatment of spraying 0.004% brassinolide with 0.25 or 7.50g Bacillus subtilis agent the dry weight of whole plant (4.59g/plant), root activity [90.31μg/(g·h) FW], the max-leaf chlorophyll content (0.5018mg/g FW) and nitrate reductase activity (0.99U/g FW) were the highest and significantly (P < 0.05) higher than that of the control; and activities of superoxide dismutase activity (56.5U/g FW) and peroxidase (2714.7U/g FW), and the content of malondialdehyde (8.8mg/g FW) were the lowest and significantly (P < 0.05) lower than that of the control. The results showed that the mixture of brassinolide and Bacillus subtilis agents could improve the quality of tobacco seedlings and cultivate strong seedlings.

Key words: Flue cured tobacco, Growth and development, Brassinolide, Bacillus subtilis

Fig.1

Dry weight and ratio of fresh to dry weight of different treatments The different small letters indicate significant difference at the 5% level"

Table 1

Chlorophyll contents of the largest leaf and root vigor of different treatments"

处理
Treatment		 根系活力
Root vigor [μg/(g·h FW)]		 叶绿素a含量
Chlorophyll a content (mg/g FW)		 叶绿素b含量
Chlorophyll b content (mg/g FW)		 叶绿素(a+b)含量
Chlorophyll (a+b) content (mg/g FW)
T1 90.30±0.50a 0.336±0.002a 0.166±0.010a 0.502±0.002a
T2 69.00±0.59d 0.295±0.002b 0.123±0.001c 0.417±0.003c
T3 81.00±0.13b 0.326±0.001a 0.136±0.001b 0.462±0.001b
T4 66.90±0.13e 0.280±0.001bc 0.110±0.001e 0.390±0.001d
T5 74.10±0.15c 0.319±0.001a 0.117±0.002d 0.437±0.002c
T6 64.10±0.17f 0.264±0.001c 0.086±0.001f 0.351±0.001e
CK 60.10±0.17g 0.199±0.034d 0.073±0.001e 0.273±0.034f

Table 2

Enzymes activities and MDA contents in the largest leaf of different treatments"

处理
Treatment		 SOD活性
SOD activity (U/g FW)		 POD活性
POD activity (U/g FW)		 CAT活性
CAT activity (U/g FW)		 NR活性
NR activity (U/g FW)		 MDA含量
MDA content (mg/g FW)
T1 56.5±1.1e 2714.7±40.7e 10.0±0.4f 0.99±0.02a 8.8±0.0g
T2 69.7±0.5c 3110.8±61.5c 32.9±0.2c 0.54±0.01d 12.1±0.0d
T3 59.2±2.0de 2934.7±47.4d 12.6±0.2e 0.86±0.01b 10.4±0.0f
T4 79.4±1.6b 3566.7±50.4b 36.9±0.2b 0.49±0.01e 12.9±0.0c
T5 61.7±1.0d 3039.3±131.2cd 13.7±0.2d 0.76±0.01c 11.4±0.0e
T6 82.0±0.7ab 3680.6±59.4b 36.9±0.3b 0.47±0.01f 13.6±0.1b
CK 84.7±2.6a 4219.6±30.0a 41.0±0.2a 0.43±0.01g 15.9±0.1a

Table 3

Correlation of dry matter content with enzyme activities and MDA content"

部位Position SOD活性
SOD activity		 POD活性
POD activity		 CAT活性
CAT activity		 NR活性
NR activity		 MDA含量
MDA content
根干重Root dry weight -0.9637** -0.9348** -0.9608** 0.9153** -0.9032**
茎干重Stem dry weight -0.8947** -0.8224** -0.7940* 0.8907** -0.9528**
叶干重Leaf dry weight -0.9136** -0.9748** -0.9161** 0.9689** -0.8610**

Table 4

Correlation between the largest leaf chlorophyll content and enzyme activities and MDA content"

指标
Index		 叶绿素a含量
Chlorophyll a
content		 叶绿素b含量
Chlorophyll b
content		 叶绿素(a+b)含量
Chlorophyll
(a+b) content
SOD活性
SOD activity		 0.903** 0.899** 0.925**
POD活性
POD activity		 0.869* 0.932** 0.918**
CAT活性
CAT activity		 0.826* 0.831* 0.851*
NR活性
NR activity		 0.838* 0.892** 0.883**
MDA含量
MDA content		 0.862* 0.956** 0.922**
[1] 李生, 李思军, 陈焘, 等. 循环运动机械化育苗方式对烤烟烟苗素质的影响. 作物研究, 2020, 34(5):444-446,458.
[2] 吴杰, 冉茂, 宗学凤, 等. 烤烟浅水育苗与漂浮育苗技术的比较研究. 西南农业学报, 2011, 24(6):2443-2445.
[3] 朱振国. 六种植物生长调节剂对烟草幼苗生长发育的影响. 泰安:山东农业大学, 2019.
[4] 梁琼月, 潘明君, 尹永强, 等. 育苗期施用复合植物生长调节剂对烤烟生长、产量及品质的影响. 甘肃农业大学学报, 2020, 55(2):98-104.
[5] 杨波, 苏鸿雁, 刘硕然. 干旱胁迫下不同植物生长调节剂对烟草幼苗抗逆性的影响. 西南农业学报, 2014, 27(6):2661-2665.
[6] 周晓艺, 薛红卫. 生长素与油菜素甾醇相互作用机制的研究进展. 中国科学:生命科学, 2013, 43(12):1047-1053.
[7] 阮英慧, 董守坤, 刘丽君, 等. 干旱胁迫下油菜素内酯对大豆花期生理特性的影响. 作物杂志, 2011(6):33-37.
[8] 孙石昂, 何发林, 姚向峰, 等. 芸苔素内酯可提高玉米幼苗的抗旱性. 植物生理学报, 2019, 55(6):829-836.
[9] Anjum S A, Wang L C, Farooq M, et al. Brassinolide application improves the drought tolerance in maize through modulation of enzymatic antioxidants and leaf gas exchange. Journal of Agronomy and Crop Science, 2011, 197(3):177-185.
doi: 10.1111/j.1439-037X.2010.00459.x
[10] 褚世海, 李林, 朱文达. 0.01%芸苔素内酯水剂对水稻生长、产量和品质的影响. 湖北农业科学, 2016, 55(24):6445-6447.
[11] 李红, 赵冰梅, 吴文忠, 等. 芸乐收对棉花抗逆增产效果. 中国棉花, 2018, 45(5):23-25.
[12] 孙晓, 姜兴印, 姚晨涛, 等. 3种不同结构芸苔素内酯在棉花上的应用研究. 中国农学通报, 2019, 35(11):121-126.
[13] 陆宁海, 郎剑锋, 张俊伟, 等. 芸苔素内酯对小麦种子萌发、幼苗生长及茎基腐病的影响. 河南科技学院学报(自然科学版), 2015, 43(3):31-35.
[14] 蒋南, 龚湛武, 陈力力, 等. 施用枯草芽孢杆菌的土壤养分含量与三大微生物间灰色关联分析. 作物杂志, 2019(3):142-149.
[15] 杨超才, 朱列书, 李迪秦, 等. 不同枯草芽孢杆菌用量对植烟土壤养分含量的影响. 西南农业学报, 2018, 31(4):779-785.
[16] Shasmita, Swain H, Naik S K, et al. Comparative analysis of different biotic and abiotic agents for growth promotion in rice (Oryza sativa L.) and their effect on induction of resistance against Rhizoctonia solani:A soil borne pathogen. Biological Control, 2019(133):123-133.
[17] 杜公福, 李晓亮, 戚志强, 等. 硫酸亚铁协同枯草芽孢杆菌对辣椒疫病抑制作用研究. 植物保护, 2020, 46(5):142-149.
[18] 黄亚丽, 郑立伟, 黄媛媛, 等. 枯草芽孢杆菌菌剂不同施用方式对甜瓜土壤微生物多样性及生长的影响. 生物工程学报, 2020, 36(12):2644-2656.
[19] 易有金, 肖浪涛, 王若仲, 等. 内生枯草芽孢杆菌B-001对烟草幼苗的促生作用及其生长动态. 植物保护学报, 2007, 34(6):619-623.
[20] 汤小朋, 陈磊, 熊康宁. 有效微生物菌群在贵州喀斯特地区农业生产中的应用前景展望. 中国农业科技导报, 2020, 22(4):129-138.
doi: 10.13304/j.nykjdb.2019.0134
[21] 黄亚丽, 郑立伟, 黄媛媛, 等. 枯草芽孢杆菌菌剂不同施用方式对甜瓜土壤微生物多样性及生长的影响. 生物工程学报, 2020, 36(12):2644-2656.
[22] 肖浪涛, 王三根. 植物生理学实验技术. 北京: 中国农业出版社, 2005.
[23] 韩景峰. 烟草栽培生理. 北京: 中国农业出版社, 2003.
[24] 王慧芳, 张希, 冯小虎, 等. 不同植物生长调节剂对烤烟生长发育的影响. 作物杂志, 2021(3):173-177
[25] 孙中华, 赵铂锤, 陈仕红, 等. 枯草芽孢杆菌B67对黄瓜幼苗生长发育的影响. 中国瓜菜, 2017, 30(2):15-18.
[26] 邓闻杨, 罗学刚, 罗蓝, 等. 混合接种3种微生物对凤眼莲吸附铀的影响. 核农学报, 2018, 32(9):1864-1871.
doi: 10.11869/j.issn.100-8551.2018.09.1864
[27] 高玉红, 闫生辉, 邓黎黎. 逆境胁迫对甜瓜幼苗生长的影响及综合抗逆鉴定指标的筛选. 江苏农业科学, 2018, 46(15):116-1118.
[28] 温日宇, 刘建霞, 张珍华, 等. 干旱胁迫对不同藜麦种子萌发及生理特性的影响. 作物杂志, 2019(1):121-126.
[29] 李安, 舒健虹, 刘晓霞, 等. 干旱胁迫下枯草芽孢杆菌对玉米种子抗旱性及生理指标的影响. 作物杂志, 2021(6):217-223.
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