Crops ›› 2023, Vol. 39 ›› Issue (2): 238-244.doi: 10.16035/j.issn.1001-7283.2023.02.034

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Screening and Identification of Biocontrol Fungi against Hirschmanniella sp. and Analysis of Nematocidal Activity

Jia Luming(), Mo Aisu, Mo Yixue, Wu Haiyan()   

  1. Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety/Agricultural College of Guangxi University, Nanning 530004, Guangxi, China
  • Received:2021-08-04 Revised:2021-10-13 Online:2023-04-15 Published:2023-04-11

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Abstract:

Hirschmanniella sp. is common in all rice regions of the world, which causes some economic losses of rice production. From the nine strains with good virulence against root-knot nematodes, we selected the strains with good virulence against of rice latent root nematodes. The results showed that ZW-1 and XO-2 had high nematocidal activities against Hirschmanniella sp., and the mortality rate in 2.5×dilution for 72h were 93.7% and 96.7%, respectively, with LC50 values of 90.4451 and 76.3875μL/mL, respectively. Strains ZW-1 and XO-2 were identified as Aspergillus japonicus and A.niger, respectively, based on morphological and rDNA-ITS molecular analysis. The discovery of two biocontrol fungal strains provides new microbial resources for the biological control of plant nematode diseases.

Key words: Hirschmanniella sp., Biocontrol fungus, Screening, Nematocidal activity

Table 1

Mortality rate of Hirschmanniella sp. exposure to 2.5 times dilution of Czapek culture solution %"

处理
Treatment		 处理时间Incubation time
24h 48h 72h
2.5倍稀释液2.5 times dilution 0.00±0.00a 0.00±0.00a 0.74±0.74a
水Water 0.00±0.00a 0.00±0.00a 0.00±0.00a

Fig.1

Mortality rates of Hirschmanniella sp. exposure to five times dilution of fermentation filtrates with different duration time Different letters indicate significant difference (P < 0.05). The same below"

Fig.2

The shape of nematode exposure to five times ZW-1 and XO-2 fermentation filtrates after 72h a: normal shape of Hirschmanniella sp.; b-c: the shapes of Hirschmanniella sp. treated with ZW-1 and XO-2 fermentation filtrates; bar=100μm"

Fig.3

The morphology of ZW-1 strain a: colony morphology after one week on PDA; b-c: spore morphology; bar=20μm"

Fig.4

The morphology of XO-2 stain a: colony morphology after one week on PDA; b-c: spore morphology; bar=20μm"

Fig.5

Mortality rate of Hirschmanniella sp. Exposure to 2.5-fold dilution of ZW-1 strain of different time fermentation filtrates"

Fig.6

Mortality rate of Hirschmanniella sp. Exposure to different dilution times of ZW-1 strain two-week fermentation filtrates"

Fig.7

Mortality rate of Hirschmanniella sp. Exposure to 2.5-fold dilution of XO-2 strain of different time fermentation filtrates"

Fig.8

Mortality rate of Hirschmanniella sp. Exposure to different dilution times of XO-2 strain two-week fermentation filtrates"

Table 2

Toxicity of ZW-1 and XO-2 strains fermentation filtrates with two-week fermenting on Hirschmanniella sp."

处理时间
Incubation time (h)		 发酵液
Fermentation filtrate		 斜率
Slope (±SE)		 相关系数
Correlation coefficient		 LC50（95%置信区间）
LC50 (95% CI) (μL/mL)
24 ZW-1 1.7206（±0.2582） 0.9678** 384.9935（235.8036~628.5741）
XO-2 1.3485（±0.1992） 0.9688** 447.8656（266.6069~752.3573）
48 ZW-1 2.3146（±0.3341） 0.9701** 136.5220（102.0527~182.6336）
XO-2 2.3538（±0.2537） 0.9923*** 128.9058（111.7788~148.6570）
72 ZW-1 2.3769（±0.2791） 0.9799** 90.4451（72.0930~113.4688）
XO-2 2.6327（±0.1341） 0.9961*** 76.3875（69.0175~84.5446）
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