Crops ›› 2023, Vol. 39 ›› Issue (5): 98-103.doi: 10.16035/j.issn.1001-7283.2023.05.014

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Effects of Dark and Strong Light Dehydration on the Photosystem Activity in Wheat Leaves in Vitro

Yang Cheng1(), Zhang Deqi1(), Du Simeng1, Zhang Lijia2, Jin Haiyang1, Li Ying1, Shao Yunhui1, Wang Hanfang1, Fang Baoting1, Li Xiangdong1(), Liu Meijun2()   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences/Henan Engineering Research Center for Wheat Yield-Quality Simultaneous Improvement/Zhengzhou University Graduate Training Base/Scientific Observing and Experimental Station of Crop Cultivation in Central Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory of Wheat Biology, Zhengzhou 450002, Henan, China
    2College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • Received:2022-03-29 Revised:2022-04-06 Online:2023-10-15 Published:2023-10-16

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Abstract:

Dehydration is the natural process of wheat leaves under drought conditions. In order to explore the mechanism of dark and strong light dehydration on photosynthetic mechanism and their differences in detached wheat leaves, the effects of dehydration with dark and strong light on the activity of wheat photosystem were determined and analyzed by using chlorophyll fluorescence dynamics technology. The results showed that the strong light accelerated the leaf dehydration process. Under the condition of dark dehydration, the maximum photochemical efficiency did not decrease significantly with the increase of dehydration degree, but decreased significantly under strong light, and the decline rate gradually accelerated with the increase of dehydration degree. Under the condition of strong light dehydration, the electron transfer from QA to QB was inhibited, and the degree of inhibition was greater than that of dark dehydration. There was no significant change in photosystem II (PSII) donor side activity under either dark or strong light dehydration conditions. The coupling degree between PSII units did not change significantly under dark conditions, but decreased significantly under strong light dehydration. Finally, it showed that the decline of PSII activity and photosynthetic performance caused by dehydration depended on light, under strong light, with the increase of dehydration degree, the absorption and transfer activity of PSII to light energy and the degree of energy coupling between PSII units gradually decline, while under dark conditions, only severe dehydration will lead to the maximum photochemical efficiency, the number of reaction centers and the electron transfer activity from QA to QB decreased slightly.

Key words: Wheat, Dehydration, Drought, Photosynthesis, Photosystem II

Table 1

Parameters and formulas of rapid chlorophyll fluorescence inducing kinetic curve"

参数Parameter 计算方法Method of calculation
FM 暗适应后照光获得的最大荧光强度
FO 叶绿素荧光诱导动力学曲线20μs的荧光强度
Fv Fv=FM?FO
Ft t时的荧光强度F
FK K点（0.3ms）的荧光强度
FJ J点（3ms）的荧光强度
FI I点（30ms）的荧光强度
VJ VJ=(FJ?FO)/(FM?FO)
VI VI=(FI ?FO)/(FM ?FO)
MO MO=4(F300μs?FO)/(FM?FO)
ΨO ψO=1?VJ
φPO φPO=Fv/FM=(FM?FO)/FM
φEO φEo=ETo/ABS=[1?(FO/FM)]?ψO
φDO φDO=1?φPO
φRO φRO=φPO×(1?VI)
σRO σRO=(1?VI)/(1?VJ)
ABS/RC ABS/RC=MO?(1/ VJ)?(1/φPO)
RC/CSm RC/CSm=φPO×(VJ/MO)×FM
PIABS PIABS=(RC/ABS)×[φPO/(1?φPO)]×[ψO/(1?ψO)]

Fig.1

RWCs of wheat leaves at different times under strong light and dark dehydration"

Fig.2

OJIP curves of wheat leaves at different times under dark and strong light dehydration"

Fig.3

O-J part of OJIP curves of wheat leaves at different times under dark and strong light dehydration"

Fig.4

Effects of dark and strong light dehydration on O-K part of OJIP curves of wheat leaves at different times"

Fig.5

Changes of fluorescence parameters in wheat leaves with different RWC under dark and strong light dehydration treatments"

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