Crops ›› 2021, Vol. 37 ›› Issue (3): 51-56.doi: 10.16035/j.issn.1001-7283.2021.03.008

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Resistance and Molecular Identification to Powdery Mildew of Pea Germplasms in Sichuan

Xiang Chao1(), Sun Suli2, Zhu Zhendong2, Zong Xuxiao2(), Yang Tao2, Liu Rong2, Yang Mei1, Xian Dongfeng1, Yang Xiuyan1   

  1. 1Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2020-12-29 Revised:2021-01-29 Online:2021-06-15 Published:2021-06-22
  • Contact: Zong Xuxiao E-mail:xc2011cib@163.com;zongxuxiao@caas.cn

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Abstract:

Powdery mildew caused by Erysiphe pisi D. C. is one of the most important diseases of pea. The most economical, effective and environmentally friendly way to control powdery mildew of pea is to plant resistant varieties. A total of 400 pea germplasm resources were identified for the resistance to powdery mildew under greenhouse conditions. At the same time, seven molecular markers linked to known powdery mildew resistance genes of pea were used for genotype identification. The results showed that among the 400 identified germplasms, eight accessions showed immunity, three accessions showed resistance, five accessions showed resistance-susceptibility separation, and the remaining 384 accessions were susceptible. Among the 16 resistant accessions, ten accessions were from different latitudes in central of Sichuan and the remaining six accessions were imported from abroad. Seven molecular markers divided the 400 germplasms into 39 marker genotypes and 16 resistant germplasms were divided into seven marker genotypes. The resistant resources and their marker genotypes described above could be effectively used for the breeding of pea resistant to powdery mildew.

Key words: Pea, Powdery mildew, Germplasm resources, Resistance identification

Table 1

Molecular markers for genotyping pea germplasms"

分子标记
Molecular
marker		 引物序列
Primer sequence		 退火温度
Annealing temperature (°C)		 遗传距离
Genetic
distance (cM)		 条带大小
Band
size (bp)		 基因
Gene		 来源文献
Reference
ScOPD-10650
 F: GGTCTACACCTAAACAGTGTCCGT
R: GGTCTACACCTCATATCTTGATGA		 58.0 2.1 650 er1 Timmerman等[17],Tiwari等[18]
ScOPE-161600
 F: GGTGACTGTGGAATGACAAA
R: GGTGACTGTGACAATTCCAG		 55.0 4.0±2.0 1600 er1 Tiwari等[18]
ScOPO-181200
 F: CCCTCTCGCTATCCAATCC
R: CCTCTCGCTATCCGGTGTG		 60.0 0.0 1200 Er1 Tiwari等[18]
ScOPX-04880
 F: CCGCTACCGATGTTATGTTTG
R: CCGCTACCGAACTGGTTGGA		 58.0 0.6 880 Er1 Srivastava等[19]
ScX17_1400
 F: GGACCAAGCTCGGATCTTTC
R: GACACGGACCCAATGACATC		 65.0 2.6 1400 er2 Katoch等[20]
SCW4637
 F: CAGAAGCGGATGAGGCGGA
R: CAGAAGCGGATACAGTACTAAC		 55.0 0.0 637 Er3 Fondevilla等[21]
SCAB1874
 F: CCGTCGGTAGTAAACTA
R: CCGTCGGTAGCCACACCA		 59.5 2.8 874 Er3 Fondevilla等[21]

Fig.1

Comparison of resistant, immune and susceptible plants"

Table 2

Sichuan resistant resources distribution"

名称
Name		 编号
No.		 抗性
Resistance		 地点
Location
昭化麻-1 Zhaohuama-1 179 R/S 昭化
绵阳花Mianyanghua 209 R/S 绵阳
金堂黄麻-2 Jintanghuangma-2 329 I 金堂
大邑白豌豆Dayibaiwandou 382 I 大邑
眉山菜Meishancai 248 R/S 眉山
犍为大绿Qianweidalü 256 I 犍为
高县大麻-2 Gaoxiandama-2 168 R 高县
会理大菜豌Huilidacaiwan 100 R 会理
会理大白Huilidabai 98 R/S 会理
会理大白壳Huilidabaike 99 R/S 会理

Fig.2

Fragments amplified by seven SCAR markers linked to the powdery mildew resistant genes 369: Shijiaxiaocaiwan No.3, 370: Shijiatiancuiwan No.1-1, 371: Chengwan No.7, 372: Shijiadacaiwan No.1-2, 373: Shijiatiancuiwan No.1-2, 374: Chengwan No.8 (control), 375: Chengwan No.9, 376: Qingwandou, M: DL 2000 Marker"

Table 3

Number of accessions with amplified fragment of seven molecular markers"

标记
Marker		 扩增出条带数
Number of accessions
with amplified fragment		 占比
Proportion
(%)		 基因
Gene
ScOPD-10650 365 91.25 er1
ScOPE-161600 334 83.50 er1
ScOPO-181200 350 87.50 Er1
ScOPX-04880 367 91.75 Er1
ScX17_1400 359 89.75 er2
SCW4637 88 22.00 Er3
SCAB1874 394 98.50 Er3

Fig.3

Cluster analysis of molecular marker genotyping of 400 pea germplasms resistance to powdery mildew G1-G39 represent 39 marker genotypes, the first number in bracket represents the number of resources, the second number represents the number of resistant resources, and the red line represents the presence of resistant resources"

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