Crops ›› 2021, Vol. 37 ›› Issue (3): 84-90.doi: 10.16035/j.issn.1001-7283.2021.013

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Effects of Genotype, Nitrogen Application Rate and Their Interactions on Polyphenols in Flue-Cured Tobacco

Zhang Xi(), Wang Huifang, Dai Zhuoyi, Xue Gang, Xu Shixiao, Yang Tiezhao()   

  1. College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2020-09-10 Revised:2020-10-22 Online:2021-06-15 Published:2021-06-22
  • Contact: Yang Tiezhao E-mail:xiaoxi59065166@126.com;yangtiezhao@126.com

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Abstract:

To explore the effects of genotypes, nitrogen application rates, and their interactions on flue-cured tobacco polyphenols, a two-factor randomized block experiment was conducted with three flue-cured tobacco varieties, Zhongyan 100, Yunyan 87, and K326, and three nitrogen levels, low nitrogen (45kg/ha), medium nitrogen (60kg/ha) and high nitrogen (75kg/ha) was used as treatments to study their effects on the contents of total phenols, chlorogenic acid, rutin and scopoletin in tobacco leaves. The results showed that genotypes, nitrogen application rates, and their interactions contributed differently to the variation of flue-cured tobacco total phenols, chlorogenic acid, rutin, and scopoletin. The contribution rates of genotypes were 2.50%, 5.13%, 4.70% and 15.95%; The contribution rates of nitrogen application rates were 59.30%, 42.55%, 38.30%, and 67.79%; genotype and nitrogen application rate interaction were 36.40%, 51.20%, 51.50%, and 15.03%. The results of the interaction effect between genotype and nitrogen application rate showed that the contents of total phenols, chlorogenic acid, and rutin were higher under the conditions of high nitrogen and Zhongyan 100, and the contents of total phenols and rutin of K326 were higher. The contents of total phenols, chlorogenic acid, and rutin of Yunyan 87 were higher under medium nitrogen conditions, while those of total phenols, rutin, and scopoletin were higher when interacting with K326. The chlorogenic acid content of Yunyan 87 was higher under the condition of low nitrogen, . It is an important measure to increase the content of polyphenols in tobacco leaves by applying suitable nitrogen application rates for planting varieties.

Key words: Genotype, Nitrogen application rate, Interaction, Flue-cured tobacco, Polyphenols

Table 1

Analysis of variance of total phenol content"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of square		 均方
Evenly split		 F F0.05 F0.01 贡献率
Contribution rate (%)
基因型Genotype 2 0.871 0.435 10.845 3.634 6.226 2.50
施氮量Nitrogen application rate 2 21.084 10.542 262.626 3.634 6.226 59.30
基因型×施氮量
Genotype×nitrogen application rate		 4 12.931 3.233 80.532 3.007 4.773 36.40
误差Error 16 0.642 0.040 1.80
总变异Total variation 26 35.587

Table 2

The total phenol contents of flue-cured tobacco leaves between different genotypes and nitrogen application rates mg/g"

项目Item 平均值Average
施氮量Nitrogen application rate N1 14.14c
N2 16.26a
N3 15.58b
基因型Genotype 中烟100 15.52a
云烟87 15.38a
K326 15.09b

Table 3

Interaction effects of genotypes and nitrogen application rates"

施氮量
Nitrogen
application rate		 中烟100
Zhongyan 100		 云烟87
Yunyan 87		 K326 N主效应
N main
effect
N1 -0.27 0.71 -0.44 -1.19
N2 -0.66 0.60 0.05 0.93
N3 0.93 -1.31 0.38 0.25
基因型主效应
Genotype main effect		 0.19 0.05 -0.24

Fig.1

Difference of total phenol content in different nitrogen application rates Different letters indicate significant difference at the level of 0.05, the same below"

Table 4

Analysis of variance of chlorogenic acid content"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of square		 均方
Evenly split		 F F0.05 F0.01 贡献率
Contribution rate (%)
基因型Genotype 2 0.769 0.384 36.754 3.634 6.226 5.13
施氮量Nitrogen application rate 2 6.374 3.187 304.708 3.634 6.226 42.55
基因型×施氮量
Genotype×Nitrogen application rate		 4 7.649 1.912 182.809 3.007 4.773 51.20
误差Error 16 0.167 0.010 1.12
总变异Total variation 26 15.009

Table 5

Chlorogenic acid content of flue-cured tobacco leaves with different genotypes and nitrogen application rates mg/g"

项目Item 平均值Average
施氮量Nitrogen application rate N1 8.31c
N2 9.49a
N3 9.05b
基因型Genotype 中烟100 8.95b
云烟87 9.16a
K326 8.75c

Table 6

Interaction effects between genotypes and nitrogen application rates"

施氮量
Nitrogen
application rate		 中烟100
Zhongyan 100		 云烟87
Yunyan 87		 K326 N主效应
N main
effect
N1 0.14 -0.30 -0.47 -0.64
N2 -0.86 0.94 0.46 0.54
N3 0.73 -0.64 0.01 0.10
基因型主效应Genotype main effect 0.01 0.21 -0.20

Fig.2

Difference of chlorogenic acid content in different nitrogen application rates"

Table 7

Analysis of variance of rutin content"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of square		 均方
Evenly split		 F F0.05 F0.01 贡献率
Contribution rate (%)
基因型Genotype 2 0.333 0.167 6.842 3.634 6.226 4.70
施氮量Nitrogen application rate 2 2.723 1.361 55.884 3.634 6.226 38.30
基因型×施氮量
Genotype×Nitrogen application rate		 4 3.662 0.915 37.581 3.007 4.773 51.50
误差Error 16 0.390 0.024 5.50
总变异Total variation 26 7.110

Table 8

Rutin content of flue-cured tobacco leaves with different genotypes and nitrogen application rates mg/g"

项目Item 平均值Average
施氮量Nitrogen application rate N1 5.61b
N2 6.33a
N3 6.22a
基因型Genotype 中烟100 6.19a
云烟87 5.92b
K326 6.06ab

Table 9

Interaction effect between genotype and nitrogen application rate"

施氮量
Nitrogen
application rate		 中烟100
Zhongyan 100		 云烟87
Yunyan 87		 K326 N主效应
N main
effect
N1 -0.39 -0.08 0.03 -0.44
N2 0.16 0.56 -0.44 0.28
N3 0.24 -0.48 0.41 0.17
基因型主效应
Genotype main effect		 0.13 -0.14 0.01

Fig.3

Difference of rutin content in different nitrogen application rates"

Table 10

Analysis of variance of scopoletin content"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of square		 均方
Evenly split		 F F0.05 F0.01 贡献率
Contribution rate (%)
基因型Genotype 2 0.052 0.026 105.242 3.634 6.226 15.95
施氮量Nitrogen application rate 2 0.221 0.110 447.751 3.634 6.226 67.79
基因型×施氮量
Genotype×Nitrogen application rate		 4 0.049 0.012 49.776 3.007 4.773 15.03
误差Error 16 0.004 0 1.23
总变异Total variation 26 0.326

Table 11

Scopoletin content of flue-cured tobacco leaves with different genotypes and nitrogen application rates mg/g"

项目Item 平均值Average
施氮量Nitrogen application rate N1 0.22c
N2 0.44a
N3 0.29b
基因型Genotype 中烟100 0.38a
云烟87 0.30b
K326 0.28b

Table 12

Interaction effect between genotype and nitrogen application rate"

施氮量
Nitrogen
application rate		 中烟100
Zhongyan 100		 云烟87
Yunyan 87		 K326 N主效应
N main
effect
N1 -0.02 0.01 0.01 -0.10
N2 0.05 -0.08 0.03 0.12
N3 -0.03 0.07 -0.04 -0.01
基因型主效应Genotype main effect 0.06 -0.02 -0.04

Fig.4

Difference of scopoletin content in different nitrogen application rates"

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