Crops ›› 2022, Vol. 38 ›› Issue (4): 214-220.doi: 10.16035/j.issn.1001-7283.2022.04.030

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Effects of Exogenous SLs and Nano-K2MoO4 on Seed Germination of Brassica napus L. under Drought Stress

Pang Xingyue(), Wan Lin, Li Su, Wang Yuhang, Liu Chen, Xiao Xiaolu, Li Xinhao, Ma Ni()   

  1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2021-04-22 Revised:2021-07-10 Online:2022-08-15 Published:2022-08-22
  • Contact: Ma Ni E-mail:pangxingyue58@qq.com;mani@caas.cn

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Abstract:

Drought stress is one of the major abiotic constraints affecting rapeseed (Brassica napus L.) germination and growth. Using Q2 (drought-resistant) and Qinyou 8 (sensitive) as materials, the effects of drought on germination and the physiological mechanmism of seed germination under drought treated by exogenous growth regulators strigolactones (SLs) and nanomaterials (n-K2MoO4) were explored. The findings demonstrated that drought stress significantly inhibited seed germination, SLs and n-K2MoO4 significantly increased germination rate, seedling dry weight, and cotyledon chlorophyll content, as well as superoxide dismutase and peroxidase activities and soluble sugar, proline, and decreased malondialdehyde contents. In comparison to other treatments, 0.10μmol/L GR24 with 0.24mmol/L n-K2MoO4 exhibited stronger mitigation effects on seed germination and oxidative damage. According to this study, exogenous SLs and n-K2MoO4 could increase the activities of protective enzymes and the levels of osmotic regulators during rapeseed germination, which improved the ability to withstand drought. The results indicate plant hormones and nanomaterials might be used in agriculture.

Key words: Strigolactones, Nanomaterial, Drought stress, Seed germination, Physiological mechanism

Table 1

Effects of drought and SLs、n-K2MoO4 on the germination traits of rapeseed under drought stress"

品种
Variety		 处理
Treatment		 GR (%) GI MT VI 出苗率
Rate of emergence (%)
Q2 CK1 100.00±0.00a 58.82±1.15a 1.10±0.09e 515.82±35.92b 97.78±3.85a
CK2 98.89±0.96a 30.88±0.70f 3.30±0.11b 51.46±7.43f 35.56±10.18d
T1 100.00±0.00a 53.18±1.60bc 1.48±0.13de 686.09±13.22a 73.33±2.89b
T2 100.00±0.00a 36.80±0.19e 2.73±0.05c 257.13±10.51d 62.22±11.10bc
T3 100.00±0.00a 51.25±1.25cd 1.61±0.11d 189.78±4.88e 76.11±10.72b
秦优8号 CK1 100.00±0.00a 55.40±2.92ab 1.32±0.21de 454.05±43.46c 96.67±4.41a
Qinyou 8 CK2 40.67±16.17b 8.06±1.84g 6.64±0.85a 16.55±5.95f 16.67±6.67e
T1 98.89±1.93a 39.77±0.49e 2.58±0.05c 456.9±45.99c 34.44±2.55d
T2 98.61±0.00a 31.38±3.98f 3.04±0.13bc 147.65±1.84e 44.44±12.95 d
T3 98.89±1.93a 49.39±1.40d 1.72±0.17d 186.54±57.76e 49.44±13.98cd

Table 2

Effects of drought and SLs、n-K2MoO4 on the agronomic traits of rape seedlings under drought stress"

品种
Variety		 处理
Treatment		 干重（mg/株）
Dry weight (mg/plant)		 主根长（cm/株）
Main root length (cm/plant)		 侧根长（cm/株）
Lateral root length (cm/plant)		 侧根数
Root hair number
Q2 CK1 30.90±1.59cd 7.40±0.72c 7.09±1.41a 8.00±1.31b
CK2 25.47±5.18ef 1.40±0.18g 1.12±0.40ef 2.00±0.36c
T1 37.46±2.75a 12.24±0.43a 3.17±0.85bc 10.00±2.31a
T2 36.93±1.53a 6.15±0.47d 2.12±1.49cde 3.00±1.22c
T3 35.57±1.40ab 2.96±0.07ef 1.27±0.21def 4.00±0.58c
秦优8号 CK1 26.97±0.90de 7.08±0.50cd 4.19±0.26b 7.00±1.67b
Qinyou 8 CK2 21.40±1.82f 2.05±0.41fg 0.32±0.07f 1.00±0.23c
T1 27.30±1.70de 10.87±0.77b 3.59±0.76bc 11.00±0.67a
T2 32.33±1.60bc 3.63±0.45e 2.40±0.40cd 3.00±0.50c
T3 31.10±3.30cd 3.22±1.37e 1.23±0.35def 3.00±0.58c

Table 3

Effects of drought and SLs、n-K2MoO4 on chlorophyll content of rape seedlings under drought stress mg /g FW"

处理
Treatment		 Chl a Chl b Chl a+b Chl a/Chl b
Q2 秦优8号
Qinyou 8		 Q2 秦优8号
Qinyou 8		 Q2 秦优8号
Qinyou 8		 Q2 秦优8号
Qinyou 8
CK1 8.93±0.32bc 6.78±0.66e 4.40±0.19abc 3.97±0.36bce 13.33±0.49bc 10.75±1.02d 2.03±0.04bc 1.71±0.01d
CK2 7.22±0.31e 5.47±0.37f 3.84±0.19cef 3.31±0.24f 11.06±0.37d 8.78±0.60e 1.88±0.12d 1.65±0.03e
T1 8.28±0.12cd 6.89±0.60e 4.27±0.19abc 4.10±0.29bce 12.55±0.31c 10.98±0.39d 1.94±0.06c 1.69±0.27d
T2 10.04±0.59a 7.96±0.29d 4.73±0.20a 4.77±0.88a 14.77±0.79a 12.72±0.59c 2.12±0.05a 1.71±0.35c
T3 9.45±0.42ab 5.93±0.32f 4.53±0.14ab 3.58±0.08ef 13.98±0.57ab 9.52±0.40e 2.08±0.03ab 1.65±0.06e

Fig.1

Effects of drought and SLs, n-K2MoO4 on the SOD and POD activities of oilseed rape leaves Different letters mean significant difference among treatments at the 5% level, the same below。。"

Fig.2

Effects of drought and SLs, n-K2MoO4 on the active oxygen contents of oilseed rape leaves"

Fig.3

Effects of drought and SLs, n-K2MoO4 on soluble sugar and proline contents of rape leaves"

Table 4

Correlation coefficients between seed germination parameters and physiological indexes in seedlings"

指标Index GR GI VI MT DW LRL MRL Chl SOD POD MDA H2O2 O-2· Pro SS
GR 1
GI 0.731**
VI 0.462* 0.683**
MT -0.836** -0.965** -0.626**
DW 0.591** 0.428* 0.455* -0.514**
LRL 0.067 0.438* 0.650** -0.303 -0.066
MRL 0.211 0.357 0.878** -0.330 0.227 0.561**
Chl 0.543** 0.334 0.339 -0.389 0.727** 0.115 0.183
SOD 0.201 -0.027 -0.124 -0.064 0.470* -0.585** -0.177 0.108
POD 0.287 0.154 -0.056 -0.198 0.519** -0.489** -0.154 0.080 0.744**
MDA -0.817** -0.925** -0.530** 0.939** -0.511** -0.361 -0.169 -0.506** -0.089 -0.169
H2O2 -0.629** -0.862** -0.720** 0.844** -0.478** -0.658** -0.417* -0.486** 0.126 -0.027 0.883**
O-2· -0.889** -0.890** -0.645** 0.935** -0.672** -0.322 -0.358 -0.670** -0.105 -0.232 0.927** 0.852**
Pro 0.308 0.062 -0.136 -0.146 0.576** -0.596** -0.234 0.320 0.909** 0.789** -0.188 0.081 -0.260
SS 0.181 -0.130 -0.185 0.040 0.453* -0.647** -0.165 0.280 0.887** 0.562** -0.023 0.195 -0.115 0.891** 1
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