Crops ›› 2024, Vol. 40 ›› Issue (4): 33-42.doi: 10.16035/j.issn.1001-7283.2024.04.005

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Genome-Wide Identification and Expression Analysis of C2H2 Zinc-Finger Protein Transcription Factor Family in Sugar Beet under Cadmium Stress

Yao Qi1,2,3(), Wang Hao2,3, Xu Lingqing2,3, Xing Wang2,3, Liu Dali2,3, Lu Zhenqiang1()   

  1. 1Province Key Laboratory of Plant Gene and Biological Fermentation in Cold Regions / College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2National Beet Medium-Term Gene Bank / College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
    3Key Laboratory of Sugar Beet Genetics and Breeding / College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
  • Received:2023-05-06 Revised:2023-08-22 Online:2024-08-15 Published:2024-08-14

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Abstract:

As an emerging energy crop, sugar beet has potential advantages in the remediation of heavy metal polluted soil. Exploring the response mechanism of sugar beet under cadmium stress is of great significance for breeding new germplasm resistant to heavy metal stress and ecological restoration. Zinc-finger protein transcription factor is a widely existing gene family in plants, which takes part in plant growth and development in various aspects and responds to biological and abiotic stresses. Through genome-wide identification and analysis of physicochemical properties, gene structure, conserved motifs, chromosome location and cis-element, we found that there were 32 members of the C2H2 zinc-finger protein transcription factor family in sugar beet, which could be divided into three subfamilies. Each member had different zinc-finger domains in different numbers, and the gene structure and sequence characteristics of different subfamilies were significantly different. By analyzing the expression of C2H2 zinc-finger protein transcription factors and the regulatory network of differentially expressed genes in sugar beet under cadmium stress, we found that the expression levels of BVRB_6g137660, BVRB_8g189640, BVRB_1g000840 and BVRB_007840 were up-regulated to varying degrees under cadmium stress. Speculating that these transcription factors play a key role in improving the cadmium tolerance of sugar beet.

Key words: Sugar beet, Zinc-finger protein, Cadmium stress, Transcription factor

Table 1

Physicochemical properties and subcellular localization of C2H2 zinc-finger protein family members in sugar beet"

基因ID
Gene ID		 转录本ID
Transcript ID		 氨基酸数量
Number of amino acids		 分子量
Molecular weight (u)		 等电点
Isoelectric point		 不稳定系数
Instability index		 亚细胞定位
Subcellular localization
BVRB_1g018520 KMT00022 366 41 146.19 8.46 70.84 细胞核
BVRB_6g136840 KMT08971 534 59 773.12 9.10 59.57 细胞核
BVRB_9g212520 KMT01234 342 37 043.98 8.84 71.81 细胞外，细胞核
BVRB_5g113850 KMT10727 563 62 233.31 8.77 56.24 细胞核
BVRB_1g012990 KMT19293 456 50 064.47 5.93 60.88 细胞核
BVRB_9g206210 KMT02271 343 37 459.07 6.24 60.35 细胞核
BVRB_7g179220 KMS97061 340 37 135.81 7.75 55.44 细胞核
BVRB_8g189640 KMT03820 242 25 661.67 8.09 53.01 细胞核
BVRB_1g015270 KMT19130 275 29 083.48 8.78 60.67 细胞核
BVRB_2g037310 KMT17528 186 20 667.45 8.86 57.60 细胞核
BVRB_1g018510 KMT00021 523 56 286.76 6.37 51.56 细胞核
BVRB_7g174490 KMT05313 231 25 076.07 5.84 38.19 细胞核
BVRB_2g046350 KMS99266 543 60 544.44 7.17 39.85 细胞核
BVRB_5g111450 KMT11057 237 26 905.18 8.52 59.79 细胞核
BVRB_6g147790 KMT07654 226 25 663.52 9.10 62.68 细胞核
BVRB_5g106070 KMT11669 187 21 346.69 5.52 59.95 细胞核
BVRB_6g130850 KMT09633 208 22 645.27 8.60 47.07 细胞核
BVRB_3g067630 KMS98868 154 17 264.82 5.64 63.00 细胞核
BVRB_3g067720 KMS98878 247 27 624.67 9.20 45.64 细胞核
BVRB_007850 KMS95480 371 41 815.85 6.12 41.96 细胞核
BVRB_3g068910 KMS98817 309 34 263.02 8.70 58.91 细胞核
BVRB_3g063640 KMT15255 323 35 834.41 8.56 58.90 细胞核
BVRB_6g138130 KMT08530 1460 164 803.16 6.37 42.75 细胞核
BVRB_016580 KMS94655 176 19 758.12 6.15 48.27 细胞核
BVRB_6g141700 KMT08346 247 25 923.68 8.64 68.65 细胞核
BVRB_007840 KMS95479 414 46 178.78 8.58 53.94 细胞核
BVRB_007870 KMS95482 370 41 964.66 8.12 44.37 细胞核
BVRB_007880 KMS95483 406 45 560.31 7.52 46.30 细胞核
BVRB_1g000840 KMT20065 513 57 833.77 5.84 49.07 细胞核
BVRB_6g137660 KMT09067 559 58 440.17 8.62 72.22 细胞核
BVRB_042870 KMS64767 88 10 142.79 11.72 50.60 叶绿体，线粒体，细胞核
BVRB_5g123490 KMS97785 641 73 777.76 5.00 56.65 高尔基体，细胞核

Fig.1

Evolutionary tree of C2H2 zinc-finger protein family in sugar beet"

Fig.2

Phylogenetic tree (a), conserved motifs (b), domains (c) and gene structure (d) analysis of C2H2 zinc-finger protein family members in sugar beet"

Fig.3

Conserved motif sequences of C2H2 zinc-finger protein family members in sugar beet"

Fig.4

C2H2 zinc-finger protein phylogenetic tree of sugar beet, Arabidopsis and rice Pink is sugar beet, green is Arabidopsis, blue is rice."

Fig.5

Chromosome location of C2H2 zinc-finger protein family members in sugar beet Chromosome color indicates gene density, with higher density for redder colors and lower density for bluer colors."

Fig.6

Promoter cis-element analysis of C2H2 zinc-finger protein transcription factor in sugar beet"

Fig.7

Heatmap of C2H2 zinc-finger transcription factor expression in sugar beet"

Fig.8

Regulatory network map of C2H2 zinc-finger transcription factors and target genes in sugar beet"

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