Crops ›› 2023, Vol. 39 ›› Issue (2): 238-244.doi: 10.16035/j.issn.1001-7283.2023.02.034

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Screening and Identification of Biocontrol Fungi against Hirschmanniella sp. and Analysis of Nematocidal Activity

Jia Luming(), Mo Aisu, Mo Yixue, Wu Haiyan()   

  1. Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety/Agricultural College of Guangxi University, Nanning 530004, Guangxi, China
  • Received:2021-08-04 Revised:2021-10-13 Online:2023-04-15 Published:2023-04-11

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Abstract:

Hirschmanniella sp. is common in all rice regions of the world, which causes some economic losses of rice production. From the nine strains with good virulence against root-knot nematodes, we selected the strains with good virulence against of rice latent root nematodes. The results showed that ZW-1 and XO-2 had high nematocidal activities against Hirschmanniella sp., and the mortality rate in 2.5×dilution for 72h were 93.7% and 96.7%, respectively, with LC50 values of 90.4451 and 76.3875μL/mL, respectively. Strains ZW-1 and XO-2 were identified as Aspergillus japonicus and A.niger, respectively, based on morphological and rDNA-ITS molecular analysis. The discovery of two biocontrol fungal strains provides new microbial resources for the biological control of plant nematode diseases.

Key words: Hirschmanniella sp., Biocontrol fungus, Screening, Nematocidal activity

Table 1

Mortality rate of Hirschmanniella sp. exposure to 2.5 times dilution of Czapek culture solution %"

处理
Treatment		 处理时间Incubation time
24h 48h 72h
2.5倍稀释液2.5 times dilution 0.00±0.00a 0.00±0.00a 0.74±0.74a
水Water 0.00±0.00a 0.00±0.00a 0.00±0.00a

Fig.1

Mortality rates of Hirschmanniella sp. exposure to five times dilution of fermentation filtrates with different duration time Different letters indicate significant difference (P < 0.05). The same below"

Fig.2

The shape of nematode exposure to five times ZW-1 and XO-2 fermentation filtrates after 72h a: normal shape of Hirschmanniella sp.; b-c: the shapes of Hirschmanniella sp. treated with ZW-1 and XO-2 fermentation filtrates; bar=100μm"

Fig.3

The morphology of ZW-1 strain a: colony morphology after one week on PDA; b-c: spore morphology; bar=20μm"

Fig.4

The morphology of XO-2 stain a: colony morphology after one week on PDA; b-c: spore morphology; bar=20μm"

Fig.5

Mortality rate of Hirschmanniella sp. Exposure to 2.5-fold dilution of ZW-1 strain of different time fermentation filtrates"

Fig.6

Mortality rate of Hirschmanniella sp. Exposure to different dilution times of ZW-1 strain two-week fermentation filtrates"

Fig.7

Mortality rate of Hirschmanniella sp. Exposure to 2.5-fold dilution of XO-2 strain of different time fermentation filtrates"

Fig.8

Mortality rate of Hirschmanniella sp. Exposure to different dilution times of XO-2 strain two-week fermentation filtrates"

Table 2

Toxicity of ZW-1 and XO-2 strains fermentation filtrates with two-week fermenting on Hirschmanniella sp."

处理时间
Incubation time (h)		 发酵液
Fermentation filtrate		 斜率
Slope (±SE)		 相关系数
Correlation coefficient		 LC50（95%置信区间）
LC50 (95% CI) (μL/mL)
24 ZW-1 1.7206（±0.2582） 0.9678** 384.9935（235.8036~628.5741）
XO-2 1.3485（±0.1992） 0.9688** 447.8656（266.6069~752.3573）
48 ZW-1 2.3146（±0.3341） 0.9701** 136.5220（102.0527~182.6336）
XO-2 2.3538（±0.2537） 0.9923*** 128.9058（111.7788~148.6570）
72 ZW-1 2.3769（±0.2791） 0.9799** 90.4451（72.0930~113.4688）
XO-2 2.6327（±0.1341） 0.9961*** 76.3875（69.0175~84.5446）
[1] 周银丽, 尹体刘, 白建波, 等. 杏仁等6种植物提取液对水稻潜根线虫的抑杀作用. 湖北农业科学, 2011, 50(6):1153-1155.
[2] 尹淦鏐, 冯志新. 农作物寄生线虫的初步调查鉴定. 植物保护学报, 1981, 8(2):111-126.
[3] 胡先奇, 余敏, 林丽飞, 等. 云南水稻潜根线虫种类及生态分布研究. 中国农业科学, 2004, 37(5):681-686.
[4] 谢家廉, 杨芳, 黄文坤, 等. 近年水稻主要线虫病害的研究进展. 植物保护学报, 2017, 44(6):940-949.
[5] Campos-Garcia J, Martinez D S T, Rezende K F O, et al. Histopathological alterations in the gills of Nile tilapia exposed to carbofuran and multiwalled carbon nanotubes. Ecotoxicology and Environmental Safety, 2016, 133:481-488.
doi: 10.1016/j.ecoenv.2016.07.041 pmid: 27543744
[6] Abd-Elgawad M M M, Askary T H. Factors affecting success of biological agents used in controlling the plant-parasitic nematodes. Egyptian Journal of Biological Pest Control, 2020, 30:17.
doi: 10.1186/s41938-020-00215-2
[7] Haarith D, Kim D G, Strom N B, et al. In vitro screening of a culturable soybean cyst nematode cyst mycobiome for potential biological control agents and biopesticides. Phytopathology, 2020, 110:1388-1397.
doi: 10.1094/PHYTO-01-20-0015-R pmid: 32286919
[8] Wu H Y, Zhang L Y, Zhou X B. Effects of Myrothecium verrucaria ZW-2 fermentation filtrates on various plant-parasitic nematodes. Journal of Plant Diseases and Protection, 2020, 127:545-552.
doi: 10.1007/s41348-020-00336-8
[9] 段玉玺, 靳莹莹, 王胜君, 等. 生防菌株Snef85的鉴定及其发酵液对不同种类线虫的毒力. 植物保护学报, 2008, 35(2):132-136.
[10] Kiewnick S, Sikora R A. Evaluation of Paecilomyces lilacinus strain 251 for the biological control of the northern root-knot nematode Meloidogyne hapla chitwood. Nematology, 2006, 8:69-78.
doi: 10.1163/156854106776179926
[11] 孙漫红, 刘杏忠, 晋治波. 淡紫拟青霉对大豆胞囊线虫卵及2龄幼虫的影响. 植物保护学报, 2002, 29(1):57-61.
[12] 张壤心, 梁英辉, 逯昕明, 等. 杀松材线虫活性菌株筛选及活性成分分析. 吉林农业大学学报, 2018, 40(2):157-163.
[13] Lee S I, Lee K J, Chun H H, et al. Process development of oxalic acid production in submerged culture of Aspergillus niger F22 and its biocontrol efficacy against the root-knot nematode Meloidogyne incognita. Bioprocess and Biosystems Engineering, 2017, 41:345-352.
doi: 10.1007/s00449-017-1867-y
[14] Jin C Z, Liu G Y, Liu X, et al. Nematicidal activity of ten kinds of plant extract against Hirschmanniella spp.. Agricultural Science and Technology, 2014, 15(12):2164-2166,2200.
[15] 周银丽, 胡先奇, 白建波, 等. 几种植物提取液对水稻潜根线虫的室内抑杀作用. 江苏农业科学, 2012, 40(3):103-104.
[16] Shemshura O N, Bekmakhanova N E, Mazunina M N, et al. Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus. Fems Microbiol Letters, 2016, 363(5):fnw026.
doi: 10.1093/femsle/fnw026
[17] Jin N, Liu S M, Peng H, et al. Isolation and characterization of Aspergillus niger NBC 001 underlying suppression against Heterodera glycines. Scientific Reports, 2019, 9:591.
doi: 10.1038/s41598-018-37827-6 pmid: 30679719
[18] He Q, Wang D Y, Li B X, et al. Nematicidal evaluation and active compounds isolation of Aspergillus japonicus ZW 1 against root-knot nematodes Meloidogyne incognita. Agronomy, 2020, 10:1222.
doi: 10.3390/agronomy10091222
[19] Jang J Y, Choi Y H, Shin T S, et al. Biological control of Meloidogyne incognita by Aspergillus niger F 22 producing oxalic acid. PLoS ONE, 2016, 11:e0156230.
doi: 10.1371/journal.pone.0156230
[20] Li J, Zou C G, Xu J P, et al. Molecular mechanisms of nematode- nematophagous microbe interactions:basis for biological control of plant-parasitic nematodes. Annual Review of Phytopathology, 2015, 53:67-95.
doi: 10.1146/phyto.2015.53.issue-1
[21] Naz I, Khan R A A, Masood T, et al. Biological control of root knot nematode, Meloidogyne incognita, in vitro, greenhouse and field in cucumber. Biological Control, 2020, 152:104429.
doi: 10.1016/j.biocontrol.2020.104429
[22] Affokpon A, Coyne D L, Htay C C, et al. Biocontrol potential of native Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biology and Biochemistry, 2011, 43:600-608.
doi: 10.1016/j.soilbio.2010.11.029
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