Crops ›› 2025, Vol. 41 ›› Issue (2): 40-46.doi: 10.16035/j.issn.1001-7283.2025.02.006

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Cultivation of New Wheat Lines with Low Lipoxygenase (LOX) Activity and Storage Tolerance by Anther Culture Technology

Zhao Yuanling1(), Tan Weiwei2, Liu Zhaojun2, Li Tie1, Li Dongmei1, Sun Minglong1, Gao Fengmei1, Wang Yongbin2()   

  1. 1Crop Resources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2024-02-18 Revised:2024-04-24 Online:2025-04-15 Published:2025-04-16

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Abstract:

The aging and deterioration of wheat during storage process will cause serious economic losses. Seven main varieties or intermediate materials in Heilongjiang province, were hybridized with three transgenic wheat materials with low lipoxygenase activity by using RNAi technology. In the F1-F3 generation materials of ten combinations respectively, six new wheat lines with excellent agronomic traits and low LOX activity were rapidly cultivated by using anther culture technology suitable for spring wheat in Heilongjiang Province, which enabled rapid transfer of excellent genes.

Key words: Wheat, Lipoxygenase, Anther culture, Rapid introgressive hybridization

Fig.1

Comparison of LOX activities between the transgenic materials and the major varieties as well as excellent lines “**”indicates extremely significant difference."

Fig.2

The results of PCR amplification of part F2 plants M: DNA Marker (DL2000); 1-21: F2 plants; 22: positive control Zhuan 4; 23: negative control Longfumai 12; 24: H2O."

Fig.3

The breeding process of anther culture in wheat (a) Microspores at the late uninucleate stage; (b) Embryos; (c) Regenerated seedling; (d) Transplanting plants; (e) Seed-setting plants; (f) H1 plants."

Table 1

Efficiency of anther culture in different years"

年份Year 穗数Spikes 花药数Anthers 愈伤数Calluses 绿苗数Green plants 成活植株数Surviving plants 结实株数Seed-setting plants
2010 65 2921 1093 544 347 67
2011 489 18 889 1776 775 305 99
2012 307 16 385 1146 551 337 93

Table 2

Characteristics of anther culture from different hybrids %"

世代
Generation		 组合
Hybrid		 出愈率
Callus induction
rate		 绿苗分化率
Green seedling
regeneration rate		 绿苗产率
Green seedling
productivity		 成活率
Survival
rate		 加倍率
Doubling
rate
F1 06k508×龙辐麦12×转3 36.46ab 53.38bc 17.70bc 50.61bcde 27.53bc
06k508×转3 40.16ab 49.20bc 19.12b 67.10ab 19.26cd
龙辐麦18×转3 41.08ab 63.00ab 26.20ab 78.17a 11.13de
06k508×龙辐麦12×转4 51.85a 62.53ab 33.33a 66.67ab 66.49a
06k508×龙辐麦12×转2 25.38bc 71.63a 18.31b 61.59abc 12.51de
F2 龙辐麦8×转2 9.64cd 56.14abc 5.37d 33.67e 30.31bc
龙辐麦17×转3 6.30d 36.68d 2.34d 39.44de 0.00e
龙麦33×转3 13.05cd 42.01c 5.69cd 38.34e 31.50bc
龙麦33×转4 12.74cd 40.63c 5.18d 42.27cde 31.39bc
06k508×转4 9.55cd 49.25bc 4.86d 59.48abcd 32.92bc
F3 06k508×龙辐麦12×转2 6.59d 47.23bc 3.20d 62.41abc 23.20bcd
均值 22.89 51.97 12.85 54.52 26.02

Table 3

Statistics on LOX activity of anther culture offspring in different hybrids"

组合
Hybrid		 品系总数
Total line
number		 父母本之间品系数
Line number
between the parents		 低于父本品系数
Number of lines less
than the male parents		 高于母本品系数
Number of lines more
than the female parents
（06k508×龙辐麦12号）×转3 (06k508×Longfumai 12)×Zhuan 3 16 7 9 0
06k508×转3 06k508×Zhuan 3 18 11 5 2
龙辐麦18×转3 Longfumai 18×Zhuan 3 7 2 4 1
龙麦33×转3 Longmai 33×Zhuan 3 9 5 4 0
龙麦33×转4 Longmai 33×Zhuan 4 11 11 0 0
龙辐麦8号×转2 Longfumai 8×Zhuan 2 1 1 0 0
合计Total 62 37 22 3
均值Mean value (%) 59.68 35.48 4.84

Fig.4

Comparison of LOX activities between superior lines and parents"

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