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作物杂志,2024, 第6期: 218–225 doi: 10.16035/j.issn.1001-7283.2024.06.029

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

水稻BR受体突变株Fn189拔节期生长发育及碳代谢分析

胡娅晴1,2(), 李春情2, 王冠1, 徐江2()   

  1. 1吉林农业大学农学院，130118，吉林长春
    2中国农业科学院作物科学研究所/农业农村部作物生理生态重点实验室，100081，北京
  • 收稿日期:2024-03-10 修回日期:2024-04-24 出版日期:2024-12-15 发布日期:2024-12-05
  • 通讯作者: 徐江，主要从事作物生理生化研究，E-mail：jiangxu_xj@163.com
  • 作者简介:胡娅晴，主要从事作物生理生化研究，E-mail：huyaqing2021@163.com
  • 基金资助:
    国家自然科学基金(31571589);中国农业科学院创新工程(01-ICS-20)

Analysis of Growth, Development and Carbon Metabolism of Rice BR Receptor Mutant Fn189 at Jointing Stage

Hu Yaqing1,2(), Li Chunqing2, Wang Guan1, Xu Jiang2()   

  1. 1College of Agronomy, Jilin Agricultural University, Changchun 130118, Jilin, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2024-03-10 Revised:2024-04-24 Online:2024-12-15 Published:2024-12-05

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摘要：

以水稻油菜素内酯（BR）受体BRI1编码基因（OsBRI1）的突变体Fn189及其野生型Tz65为供试材料，在大田条件下，采取随机区组试验设计，研究BR对拔节期水稻生长发育及碳代谢相关生理指标的影响。结果表明，与野生型相比，Fn189拔节期生长受到抑制，Fn189的株高、分蘖数分别降低了25.07%和15.82%。此外，Fn189拔节期叶片的Rubisco活性降低，叶片固定CO2的能力减弱；在蔗糖―淀粉代谢中，与野生型相比，Fn189拔节期叶片的蔗糖磷酸合成酶、蔗糖合酶、腺苷二磷酸葡萄糖焦磷酸化酶、可溶性淀粉合酶和颗粒结合型淀粉合酶活性降低，酸性转化酶活性增加，说明Fn189叶肉细胞内蔗糖和淀粉合成减弱，而蔗糖分解加速；结果显示Fn189叶片中蔗糖、可溶性糖、淀粉和非结构性碳水化合物含量均显著降低，较野生型分别降低了23.76%、13.46%、9.02%和10.40%。最终，Fn189的产量较野生型降低了71.04%。综上，BR信号转导受阻不仅影响了拔节期水稻的生长发育，还通过影响水稻叶片的光合作用、蔗糖和淀粉代谢，最终影响水稻产量。

关键词: 水稻, 油菜素内酯, 突变体Fn189, 拔节期, 生长发育, 碳代谢

Abstract:

Rice brassinosteroids (BR) receptor BRI1 coding gene (OsBRI1) mutant Fn189 and its wild type Tz65 were used as test materials. Under field conditions, a randomized block design was used to study the effects of BR on growth and development and carbon metabolism-related physiological indexes of rice at jointing stage. The results showed that compared with the wild-type Tz65, the growth of Fn189 was inhibited at jointing stage, and the plant height and tiller number of Fn189 were decreased by 25.07% and 15.82%, respectively. In addition, the Rubisco activity of Fn189 leaves decreased at jointing stage, and the ability of leaves to fix CO2 decreased. Compared with the wild-type, the activities of sucrose phosphate synthetase, sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase and granule-bound starch synthase of Fn189 leaves decreased at jointing stage, and the activity of acid invertase increased, indicating that the synthesis of sucrose and starch in the mesophyll cells of Fn189 decreased, while the decomposition of sucrose accelerated. The results showed that the contents of sucrose, soluble sugar, starch and non-structural carbohydrates in Fn189 leaves were significantly decreased by 23.76%, 13.46%, 9.02% and 10.40%, respectively, compared with the wild-type. Finally, the yield of Fn189 was 71.04% lower than that of wild-type. Therefore, BR signal transduction not only affected the growth and development of rice at jointing stage, it also affected the photosynthesis of rice leaves and the metabolism of sucrose and starch, and ultimately affected the yield of rice.

Key words: Rice, Brassinosteroids, Mutant Fn189, Jointing stage, Growth and development, Carbon metabolism

图1

拔节期Fn189和Tz65形态指标的比较 “*”和“**”分别表示在P < 0.05和P < 0.01水平上差异显著，下同。

图2

拔节期Fn189和Tz65光合相关指标的比较

图3

拔节期Fn189和Tz65蔗糖、淀粉代谢的比较

表1

收获期Fn189和Tz65产量及其构成因素的比较

材料
Material		 有效穗数
Effective number of panicles (/m2)		 穗粒数
Grains per panicle		 千粒重
1000-grain weight (g)		 结实率
Seed-setting rate (%)		 产量
Yield (kg/hm2)
Tz65 504.00 96.73 26.56 81.56 9818.80
Fn189 381.00** 106.40* 16.97** 42.58** 2843.27**
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