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作物杂志,2024, 第1期: 180–186 doi: 10.16035/j.issn.1001-7283.2024.01.024

所属专题： 杂粮作物

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

烯效唑与褪黑素复配对红小豆萌发和根系形态的影响

刘佳1,2(), 吴天一1, 朱嘉宇1, 邓绍珠1, 张玉先1,2, 梁喜龙1, 金喜军1,2()   

  1. 1黑龙江八一农垦大学农学院，163319，黑龙江大庆
    2国家杂粮工程技术研究中心，163319，黑龙江大庆
  • 收稿日期:2022-11-21 修回日期:2023-09-25 出版日期:2024-02-15 发布日期:2024-02-20
  • 通讯作者: 金喜军，研究方向为作物栽培学与耕作学，E-mail：shaoxiang1979@163.com
  • 作者简介:刘佳，研究方向为作物栽培学与耕作学，E-mail：l197209elieensbing@163.com
  • 基金资助:
    黑龙江省“揭榜挂帅”科技攻关项目(2021ZXJ05B011);黑龙江省农垦总局科技攻关项目(HNK135-02-10);黑龙江八一农垦大学研究生科研创新资助项目(YJSCX2021-Y57);农业农村部大豆机械化生产重点实验室资助

Effects of Uniconazole Combined with Melatonin on Germination and Root Morphology of Adzuki Bean

Liu Jia1,2(), Wu Tianyi1, Zhu Jiayu1, Deng Shaozhu1, Zhang Yuxian1,2, Liang Xilong1, Jin Xijun1,2()   

  1. 1College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2National Coarse Cereals Engineering Technology Research Center, Daqing 163319, Heilongjiang, China
  • Received:2022-11-21 Revised:2023-09-25 Online:2024-02-15 Published:2024-02-20
  • Contact: Jin Xijun

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摘要：

为通过植物生长调节剂处理种子提升幼苗质量，设置了烯效唑浓度为2、4、8、16和32 mg/L，及上述浓度烯效唑与100 μmol/L褪黑素复配包衣处理，以无药剂溶液包衣为对照，研究不同浓度烯效唑及其与褪黑素复配对红小豆种子萌发、根系形态特征和抗氧化酶活性、糖类含量等的影响。结果表明，8 mg/L烯效唑（S8）及其与褪黑素复配（S8M）处理红小豆发芽指数较对照均显著提高，发芽率与对照无显著差异。随着烯效唑浓度提高，根系中果糖、可溶性糖和可溶性蛋白含量呈先上升后下降的趋势，均表现为S8最高，而根系蔗糖和淀粉含量则未表现出明显规律。与其他处理相比，S8及S8M处理更有利于同时促进抗氧化酶活性维持较高水平，显著提高根系活力。相关性分析表明，上述生理指标和根系形态指标存在显著和极显著相关关系。综合分析认为，8 mg/L烯效唑包衣及其复配100 μmol/L褪黑素包衣处理均可促进红小豆萌发和根系发育，有利于出苗整齐和壮苗形成。

关键词: 红小豆, 烯效唑, 褪黑素, 萌发, 根系形态建成

Abstract:

In order to improve the quality of seedlings by treating seeds with plant growth regulators, this study set the concentration of uniconazole at 2, 4, 8, 16 and 32 mg/L, and the above concentration of uniconazole and 100 μmol/L melatonin compound coating treatment, with no drug solution coating as the control, to study the effects of different concentrations of uniconazole and its combination with melatonin on seed germination, root morphological characteristics, antioxidant enzyme activities and sugar contents of adzuki bean. The results showed that the germination index of adzuki bean treated with 8 mg/L uniconazole (S8) and its combination with melatonin (S8M) were significantly higher than that of the control, and the germination rate was not significantly different compared with control. With the increase of uniconazole concentration, the contents of fructose, soluble sugar and soluble protein in roots showed a trend of first up and then down, all of which were the highest in S8 treatment, while the contents of sucrose and starch in roots did not show obvious regularity. Compared with other treatments, S8 and S8M treatments were more conducive to maintaining a high level of antioxidant enzyme activities and significantly improving root activity. Correlation analysis showed that there were significant and extremely significant correlations between the above physiological indexes and root morphological indexes. Comprehensive analysis showed that 8 mg/L uniconazole coating and its combination with 100 μmol/L melatonin coating could promote the germination and root development of adzuki bean, which was conducive to the emergence of neat and strong seedlings.

Key words: Adzuki bean, Uniconazole, Melatonin, Germination, Root morphogenesis

表1

包衣成分

处理Treatment 药剂及浓度Reagent and concentration
CK S3307 0 mg/L+MT 0 μmol/L
S2 S3307 2 mg/L
S2M S3307 2 mg/L+MT 100 μmol/L
S4 S3307 4 mg/L
S4M S3307 4 mg/L+MT 100 μmol/L
S8 S3307 8 mg/L
S8M S3307 8 mg/L+MT 100 μmol/L
S16 S3307 16 mg/L
S16M S3307 16 mg/L+MT 100 μmol/L
S32 S3307 32 mg/L
S32M S3307 32 mg/L+MT 100 μmol/L

图1

不同浓度烯效唑与褪黑素复配对红小豆萌发的影响 箱线图中箱体的上、下底分别代表数据的上四分位数（Q3）和下四分位数（Q1），箱体中横线为数据中位数（Q2）；上、下边缘分别代表该组数据的最大值和最小值；点代表异常值；不同字母表示不同处理间差异显著（P<0.05)。下同。

图2

不同浓度烯效唑与褪黑素复配对红小豆根系糖和可溶性蛋白含量的影响

图3

不同浓度烯效唑与褪黑素复配对红小豆根系抗氧化酶活性的影响

图4

不同浓度烯效唑与褪黑素复配对红小豆根系形态和活力的影响

图5

生理指标与根系形态和活力相关性分析 SU：蔗糖含量；FR：果糖含量；SS：可溶性糖含量；ST：淀粉含量；SP：可溶性蛋白含量；RL：根长；RSA：根表面积；RD：根直径；RV：根体积；NLR：侧根数；RA：根系活力。“*”代表相关性显著（P < 0.05），“**”代表相关性极显著（P < 0.01），“***”代表相关性极显著（P < 0.001）。

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