Crops ›› 2016, Vol. 32 ›› Issue (3): 33-36.doi: 10.16035/j.issn.1001-7283.2016.03.007

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The Study on the Influence Factors of Callus and Adventitious Bud Induction in Pea

Zhang Lijuan,Lu Jianying,Wang Chang,Min Gengmei,Yang Xiaoming   

  1. Crop Institute,Gansu Academy of Agricultural Sciences,Gansu 730070,Lanzhou,China
  • Received:2016-01-18 Revised:2016-04-02 Online:2016-06-15 Published:2018-08-26

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Abstract:

The pea variety Longwan No.1 was used as experimental material to study the factors in callus and adventitious bud induction process, such as seed treatment,explants selection, optimal medium, adventitious bud and rooted inducing.The results showed that the optimal disinfection treatments for seed was 75% alcohol solution for 30 seconds, and then 0.1% HgCl2 for 10min. Hypocotyl as the explants, the optimal culture medium of callus was 0.2mg/L 2,4-D and 1.0mg/L TDZ with an induction rate of 91.11%. Only the addition of TDZ could induce adventitious bud,but the rooting rate was only 8.89%. We also analyzed the factors affecting the growth of root,which provided the basis for subsequent research.

Key words: Pea, Callus, Adventitious shoot

Table 1

Effects of different disinfection treatment on seed germination"

处理
Treatments		 75%乙醇消毒时间(s)
Disinfection time of 75% alcohol solution		 0.1%升汞消毒时间(min)
Disinfection time of0.1% HgCl2		 出苗率(%)
Emergence rate		 污染情况
Condition of contamination
C1 30 0 14.44±1.11g +++
C2 30 5 54.45±2.22e ++
C3 30 10 92.22±2.94a -
C4 30 15 93.33±1.93a -
C5 30 20 84.44±1.11b -
C6 30 30 51.11±1.11e -
C7 60 0 27.78±2.94f +++
C8 60 5 70.00±1.92d +
C9 60 10 90.00±1.92ab -
C10 60 15 87.78±1.11ab -
C11 60 20 76.67±1.93c -
C12 60 30 48.89±1.11e -

Table 2

Effects of different explants on callus induction"

外植体Explants 接种外植体数No.of explants(个) 产生愈伤组织外植体数No.of Callus induction(个) 愈伤组织诱导率Callus induction rate(%)
下胚轴Hypocotyl 90×3 224 82.97±1.34aA
子叶Cotyledon 90×3 64 23.70±0.74bB
胚根Radicel 90×3 17 6.30±1.34cC

Table 3

Effect of different hormones on callus induction of hypocotyl"

组合代号
Code of combination		 2,4-D浓度(mg/L)
2,4-D concentration		 6-BA浓度(mg/L)
6-BA concentration		 TDZ浓度(mg/L)
TDZ concentration		 愈伤组织诱导率(%)
Callus induction rate
Y1 0.2 0 0 0.00±0.00e
Y2 1.0 0 0 0.00±0.00e
Y3 0.2 0.2 0 57.78±2.94d
Y4 0.2 0.5 0 74.44±2.94c
Y5 0.2 0 0.5 83.33±1.93b
Y6 0.2 0 1.0 91.11±1.11a

Table 4

Effect of TDZ on adventitious shoot differentiation"

TDZ浓度(mg/L)
TDZ concentration		 接种愈伤组织块数
No.of callus		 形成不定芽的愈伤组织块数
No.of callus with shoots		 不定芽分化率(%)
Shoot differentiation rate		 不定芽增殖数(个)
No.of shoots per explant
0.5 30×3 75 83.33±3.85a 10.00±1.15c
1.0 30×3 81 90.00±1.92a 17.67±1.20c
2.0 30×3 82 91.11±2.94a 25.00±2.52b
4.0 30×3 82 91.11±1.11a 26.33±0.88a

Table 5

Effect of different hormones on root induction of adventitious shoot"

激素浓度(mg/L)
Hormone concentration		 生根率 Rooting rate(%)
NAA IBA IAA
0.5 0 0 0
1.0 0 0 0
2.0 0 8.89 0
4.0 0 0 0
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