Crops ›› 2016, Vol. 32 ›› Issue (6): 33-37.doi: 10.16035/j.issn.1001-7283.2016.06.006

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Combining Ability Analysis of Verticillium Wilt-Resistance in Five Resistant Cultivars

Zhu Xiefei1,Li Shenghua2,Wang Sen1   

  1. 1 Cotton Institute,Nanjing Agricultural University/State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,Jiangsu,China
    2 Shengdi Institute of Industrial Crops of Suiyang District,Shangqiu 476025,Henan,China
  • Received:2016-06-23 Revised:2016-10-14 Online:2016-12-15 Published:2018-08-26

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Abstract:

Five disease-resistant upland cotton cultivars as paternal parents and four susceptible cultivars as maternal parents were used, and incomplete diallel crosses were made to analyze the combining ability of verticillium wilt-resistance. The results indicated that Ji 122 and Zhongzhimian 2 had high positive general combining ability (GCA) in incidence, disease index, and relative disease index,while Jimian 616, Yumian 112, and Xumian 18 had the negative effects. Special combining ability (SCA) analysis indicated that the above traits showed positive effects in nine crosses and negative effects in eleven crosses. Analysis of variance from GCA and SCA showed three traits (incidence, disease index, and relative disease index) were dominated by additive effects, implying that these traits had high broad-sense heritability and could be selected at early generations.

Key words: Cotton, Cultivar, Verticillium, Combining ability

Table 1

The field resistance identifying to vertillium wilt for parents and crosses"

材料名称Material name 发病率Morbidity(%) 病情指数Disease index 相对病情指数Relative disease index 抗病类型Disease resistance type
B1×A1 83.33 45.56 44.65 感病
B2×A1 76.67 43.33 42.39 感病
B3×A1 48.89 26.67 26.15 耐病
B4×A1 43.33 24.17 23.72 耐病
B1×A2 60.00 31.11 30.52 耐病
B2×A2 60.00 34.44 33.61 耐病
B3×A2 40.00 23.06 22.61 耐病
B4×A2 42.22 23.61 22.96 耐病
B1×A3 48.89 26.94 26.18 耐病
B2×A3 45.56 23.89 23.46 耐病
B3×A3 40.00 23.33 22.74 耐病
B4×A3 33.33 19.17 18.66 抗病
B1×A4 84.44 46.03 45.02 感病
B2×A4 86.67 48.89 47.93 感病
B3×A4 52.22 29.72 29.14 耐病
B4×A4 66.67 38.33 37.50 感病
B1×A5 32.22 18.06 17.65 抗病
B2×A5 34.44 19.72 19.28 抗病
B3×A5 30.00 20.00 19.73 抗病
B4×A5 32.22 19.44 19.09 抗病
A1 27.78 19.17 18.76 抗病
A2 25.56 14.44 14.07 抗病
A3 24.44 15.28 14.93 抗病
A4 22.22 16.94 16.51 抗病
A5 21.11 12.22 12.00 抗病
B1 90.00 50.00 49.01 感病
B2(CK) 83.33 51.11 50.00 感病
B3 71.11 41.11 40.37 感病
B4 64.44 37.50 36.65 感病

Table 2

Analysis of variance"

变异来源Source of variation 自由度df 发病率Morbidity(%) 病情指数Disease index 相对病情指数Relative disease index
区组Between blocks 2 47.65 34.93 4.60
组合Between groups 28 1 437.37** 427.46** 410.59**
误差Error 56 34.54 16.15 15.87

Table 3

Variance of combining ability"

变异来源Source of variation 自由度df 发病率Morbidity(%) 病情指数Disease index 相对病情指数Relative disease index
区组Between blocks 2 78.01 20.57 7.17
组合Between groups 19 1 046.89** 324.09** 290.26**
母本Female 4 3 110.25** 964.58* 864.71*
父本Male 3 1 688.21** 443.96** 391.47**
父本×母本Male×Female 12 198.77** 80.62** 73.47**
误差Error 38 37.41 18.71 18.68

Table 4

Analysis results of general combining ability on vertillium wilt resistance in parents"

亲本Parents 发病率Morbidity(%) 病情指数Disease index 相对病情指数Relative disease index
冀122 Ji122 21.13 18.58 19.47
冀棉616 Jimian616 -2.88 -4.76 -4.27
豫棉112 Yumian112 -19.42 -20.79 -20.56
中植棉2号Zhongzhimian2hao 39.27 41.44 39.26
徐棉18号Xumian18hao -38.10 -34.47 -33.91
苏棉2186 Sumian2186 18.68 16.36 14.50
泗棉3号Simian3hao 16.54 15.61 16.35
中江棉8号Zhongjiangmian8hao -18.89 -16.64 -15.97
宁棉2号Ningmian2hao -16.33 -15.33 -14.87

Table 5

Analysis results of special combining ability on vertillium wilt resistance"

编号
Number		 组合
Crosses		 发病率
Morbidity(%)		 病情指数
Disease index		 相对病情指数
Relative disease index
1 B1×A1 20.28 19.70 21.88
2 B2×A1 9.61 12.93 12.15
3 B3×A1 -8.33 -11.41 -12.21
4 B4×A1 -21.56 -21.21 -21.81
5 B1×A2 -0.54 -5.98 -3.70
6 B2×A2 1.60 6.08 5.24
7 B3×A2 -1.39 -0.33 -0.85
8 B4×A2 0.32 0.23 -0.69
9 B1×A3 -5.34 -4.10 -2.58
10 B2×A3 -9.60 -13.72 -13.90
11 B3×A3 15.15 16.65 15.91
12 B4×A3 -0.21 1.18 0.58
13 B1×A4 4.27 10.99 3.39
14 B2×A4 10.67 8.91 11.67
15 B3×A4 -20.07 -23.91 -21.58
16 B4×A4 5.12 4.01 6.52
17 B1×A5 -18.68 -20.60 -18.99
18 B2×A5 -12.28 -14.19 -15.15
19 B3×A5 14.63 19.00 18.73
20 B4×A5 16.33 15.79 15.40

Table 6

Estimates of heritability and population genetic variances"

遗传参数
Genetic parameter		 发病率(%)
Morbidity		 病情指数
Disease index		 相对病情指数
Relative disease index
σ2m 242.62 73.66 65.94
σ2f 99.30 24.22 21.20
σ2f+m 341.92 97.89 87.14
σ2fm 53.79 20.64 18.27
σ2f+m/σ2fm 6.36 4.74 4.77
h2B(%) 91.36 86.37 84.95
h2N(%) 78.94 71.33 70.23
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