Crops ›› 2018, Vol. 34 ›› Issue (4): 167-174.doi: 10.16035/j.issn.1001-7283.2018.04.028

Previous Articles    

Effects of Salt Stress on Seed Germination and Physiological Characteristics of Apocynum venetum

Fang Jingwen,Wu Yan,Liu Zhihua   

  1. College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
  • Received:2017-12-11 Revised:2018-03-07 Online:2018-08-20 Published:2018-08-23

RichHTML

9

PDF (PC)

193

Abstract:

The effects of different salt levels [0 (CK), 100, 200, 300 and 400mmol/L NaCl] on physiological characteristics of seed germination of Apocynum venetum were investigated, taking Apocynum venetum seed from Bameng, Baotou, Tongliao, regions, in Inner Mongolia as test material. Salt resistance was comprehensively evaluated with salt damage index, salt damage level and subordinative function at this stage to investigate the response of varieties to salt stress. The results showed: The seed germination rate, germination index and vigor index were all significantly reduced when the salt concentration was in 0-400mmol/L NaCl; the content of malondialdehyde (MDA)in Bameng, Baotou and Tongliao areas showed a upward trend with the increase of salt concentration. However, the contents of supemxide dismutase (SOD), peroxidase (POD), proline (Pro) and soluble sugar of all three regions showed a upward trend at first and downward trend later with the increase of salt concentration evenly. At the same time, NaCl concentration less than 200mmol/L had a little influence on seed germination of Apocynum venetum. Salt resistance of Apocynum venetum seeds from 3 areas were evaluated by salt damage index, salt damage level and adapting subordinate function method in Fuzzy Mathematics. Ability of salt-resistance was: Bameng Apocynum venetum > Baotou Apocynum venetum > Tongliao Apocynum venetum.

Key words: Salt stress, Apocynum venetum, Physiological index, Subordinate function

Table 1

The location of study area and ecological factor"

地区
Area		 经度、纬度
Longitude and latitude		 平均温度(℃)
Average temperature		 降雨量(mm)
Rainfall		 气候类型
Climate type		 土壤特点
Soil characteristic
内蒙古农业大学农场
Inner Mongolia Agricultural
University farm		 E 111°42′
N 45°5′
 6.6

 400

 典型大陆性气候

 土壤为沙壤质暗栗钙土,pH 7.8~8.2,肥力适中,具有良好灌溉条件
巴盟Bameng
 E 106°9′
N 40°9′		 7.6
 147
 暖温带大陆性气候
 草甸土为主,表土层最高含盐量可达3.78%,盐碱化、沙化严重
包头Baotou
 E 109°52′
N 41°20′		 6.4
 310
 半干旱中温带
大陆性季风气候		 栗钙土、草甸土等易形成深厚的钙积层,工业污染重导致土壤盐碱化
通辽Tongliao
 E 119°14′
N 43°50′		 5.8
 400
 温带半干旱
大陆性气候		 土壤质地为壤质土、粉沙土,二者毛管性能强,使土壤积累盐分快而多

Table 2

Effects of salt stress on germination rate of Apocynum venetum seeds from different areas %"

NaCl浓度(mmol/L)
NaCl concentration		 包头
Baotou		 通辽
Tongliao		 巴盟
Bameng
CK 99.33±1.15a 98.67±1.15a 98.00±3.46a
100 99.33±1.15a 98.67±2.31a 97.33±1.15ab
200 97.33±1.15a 96.67±4.16a 96.67±1.15ab
300 92.00±2.00b 85.33±1.15b 92.67±3.06b
400 42.67±1.15c 37.33±2.31c 48.67±3.06c

Table 3

Effects of salt stress on germination index and vigor index of Apocynum venetum seeds from different areas"

NaCl浓度
(mmol/L)
NaCl concentration		 包头Baotou 通辽Tongliao 巴盟Bameng
发芽指数
Germination index		 活力指数
Vigor index		 发芽指数
Germination index		 活力指数
Vigor index		 发芽指数
Germination index		 活力指数
Vigor index
CK 29.06±0.63a 27.59±0.60a 26.89±0.59a 28.38±0.62a 26.48±0.77a 20.89±0.61a
100 23.22±0.48b 16.88±0.35b 23.44±1.13b 19.79±0.95b 23.11±0.38b 14.31±0.24b
200 20.37±0.38c 13.35±0.25c 19.76±1.12c 12.76±0.72c 22.50±0.93b 14.31±0.59b
300 13.96±0.27d 3.31±0.07d 11.98±0.48d 2.90±0.12d 13.00±0.86c 3.51±0.22c
400 4.33±0.30e 0.34±0.02e 3.97±0.28e 0.30±0.02e 5.30±0.35d 0.22±0.01d

Fig.1

Effects of salt stress on antioxidant enzyme activities of Apocynum venetum seeds from different areas"

Fig.3

Effects of salt stress on proline content and soluble sugar content of Apocynum venetum seeds from different areas"

Fig.2

Effects of salt stress on MDA content of Apocynum venetum seeds from different areas"

Table 4

Salt damage index and salt damage level of Apocynum venetum seeds from different areas"

NaCl浓度
(mmol/L)
NaCl concentration		 盐害指数(%)
Salt damage index		 盐害级别
Salt damage level
包头
Baotou		 通辽
Tongliao		 巴盟Bameng 包头
Baotou		 通辽Tongliao 巴盟Bameng
100 0.67 1.33 1.33 1级 1级 1级
200 2.00 2.67 2.00 1级 1级 1级
300 7.37 13.51 6.00 1级 1级 1级
400 57.03 62.15 50.35 3级 4级 3级

Table 5

Analysis of subordinate function values of salt tolerance indexes of the seeds of Apocynum venetum from different areas"

地区
Area		 发芽率
Germination rate		 发芽指数
Germination
index		 活力指数
Vigor
index		 SOD活性
SOD
activity		 MDA含量
MDA
content		 POD活性
POD
activity		 脯氨酸含量
Proline
content		 可溶性糖含量
Soluble sugar
content		 平均值Average value 排序
Order
巴盟Bameng 0.770 0.60 0.503 0.495 0.632 0.496 0.420 0.481 0.550 1
包头Baotou 0.767 0.56 0.440 0.488 0.656 0.479 0.360 0.349 0.512 2
通辽Tongliao 0.750 0.58 0.446 0.432 0.534 0.433 0.366 0.398 0.492 3

Table 6

Correlation analysis among parameters and salt concentration"

发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor index		 SOD 活性
SOD activity		 POD活性
POD activity		 MDA含量
MDA content		 脯氨酸含量
Proline content		 可溶性糖
Soluble sugar content
盐浓度
Salt concentration		 -0.790 -0.975** -0.986** 0.693 0.898* 0.967** 0.785 0.838
[1] 张建锋, 张旭东, 周金星 , 等. 世界盐碱地资源及其改良利用的基本措施. 水土保持研究, 2005,12(6):8.
[2] 周和平, 张立新, 禹锋 , 等. 我国盐碱地改良技术综述及展望. 现代农业科技, 2007(11):159.
doi: 10.3969/j.issn.1007-5739.2007.11.120
[3] 樊瑞苹, 周琴, 周波 , 等. 盐胁迫对高羊茅生长及抗氧化系统的影响. 草业学报, 2012,21(1):112-117.
doi: 10.11686/cyxb20120114
[4] Munns R . Comparative physiology of salt and water stress. Plant, Cell and Environment, 2002,25:239-250.
doi: 10.1046/j.0016-8025.2001.00808.x pmid: 11841667
[5] 揭雨成, 冷鹃, 许英 . 罗布麻生态特征与产业化研究进展. 中国麻业, 2001,23(3):34-37.
doi: 10.3969/j.issn.1671-3532.2001.03.009
[6] 中国植物志编辑委员会. 中国植物志: 第63卷. 北京:科学出版社, 1977.
[7] 王东清, 李国旗, 程志 . 罗布麻研究利用现状及展望. 江苏农业科学, 2011,39(3):310-313.
[8] Khan M A, Shelth K H . Effects of different levels of salinity on seeds germination and growth of Capsicum annuum L. Blologla, 1996,22:15-16.
[9] 张秀玲 . NaCl胁迫对罗布麻种子萌发的影响. 中国种业, 2007(5):48-49.
doi: 10.3969/j.issn.1671-895X.2007.05.021
[10] Parida A K, Das A B . Salt tolerance and salinity effects on plants:a review. Ecotoxicology Environmental Safety, 2005,60(3):324-349.
doi: 10.1016/j.ecoenv.2004.06.010 pmid: 15590011
[11] 赵博生, 衣艳君, 刘家尧 . 外源甜菜碱对干旱/盐胁迫下的小麦幼苗生长和光合功能的改善. 植物学通报, 2001(18):378-380.
doi: 10.3969/j.issn.1674-3466.2001.03.019
[12] 万海霞 . 盐胁迫和遮光处理对罗布麻生长及生理生化特性的影响. 银川:宁夏大学, 2008.
doi: 10.7666/d.y1681773
[13] 张紫薇, 庞春花, 张永清 , 等. 等渗NaCl和PEG胁迫及复水处理对藜麦种子萌发及幼苗生长的影响. 作物杂志, 2017(1):119-126.
doi: 10.16035/j.issn.1001-7283.2017.01.022
[14] 田鑫, 钟程, 李性苑 , 等. 盐胁迫对薏苡种子萌发及幼苗生长的影响. 作物杂志, 2015(2):140-143.
[15] 杨艳艳, 王庆祥 . 盐胁迫对不同玉米品种幼苗渗透调节物质的影响. 作物杂志, 2012(1):99-102.
doi: 10.3969/j.issn.1001-7283.2012.01.025
[16] 李占成, 张丽丽, 李玮 , 等. 盐胁迫对糜子种子发芽的影响. 作物杂志, 2011(6):122-123.
[17] 斯琴巴特尔, 吴红英 . 盐胁迫对玉米种子萌发及幼苗生长的影响. 干旱区资源与环境, 2000(4):77-81.
doi: 10.3969/j.issn.1003-7578.2000.04.014
[18] 程龙, 韩占江, 石新建 , 等. 白茎盐生草种子萌发特性及其对盐旱胁迫的响应. 干旱区资源与环境, 2015,29(3):131-136.
[19] 史树德 . 植物生理学实验指导. 北京: 中国林业出版社, 2011: 74-75.
[20] 李玲 . 植物生理学模块实验指导. 北京: 农业出版社, 2009: 97-98.
[21] 张殿忠, 汪沛洪, 赵会贤 . 测定小麦叶片游离脯氨酸含量的方法. 植物生理学通讯, 1990(4):62-65.
[22] 李合生 . 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 167-169.
[23] 陈德明, 俞仁培, 杨劲松 . 盐渍条件下小麦抗盐性的隶属函数值法评价. 土壤学报, 2002,39(3):368-373.
doi: 10.11766/trxb200101260311
[24] 柴媛媛, 史团省, 谷卫彬 . 种子萌发期甜高粱对盐胁迫的响应及其耐盐性综合评价分析. 种子, 2008,27(2):43-47.
[25] 马立群, 刘志华, 杨丽 . 内蒙古不同地区罗布麻种子形态观察与分析. 内蒙古农业科技, 2008(4):41-42.
doi: 10.3969/j.issn.1007-0907.2008.04.016
[26] 李海云, 赵可夫, 王秀峰 . 盐对盐生植物种子萌发的抑制. 山东农业大学学报(自然科学版), 2002,33(2):170-173.
[27] 徐小玉, 张凤银, 曾庆微 . NaCl和Na2SO4盐胁迫对波斯菊种子萌发的影响. 东北农业大学学报, 2014,45(4):55-59.
doi: 10.3969/j.issn.1005-9369.2014.04.011
[28] 范希峰, 候新村, 朱毅 , 等. 盐胁迫对柳枝稷苗期生长和生理特性的影响. 应用生态学报, 2012,23(6):1476-1480.
doi: 10.11733/j.issn.1007-0435.2015.02.020
[29] 李春龙 . 盐胁迫对油菜种子萌发的影响. 安徽农业科学, 2008,36(26):11198-11199.
doi: 10.3969/j.issn.0517-6611.2008.26.018
[30] 朱毅, 范希峰, 刘吉利 , 等. 盐胁迫对柳枝稷种子萌发的影响. 中国草地学报, 2014,36(4):38-43.
[31] 荆瑞英, 刘庆超, 王静 , 等. 盐胁迫对罗布麻种子萌发及幼苗生长的影响. 北方园艺, 2012(9):43-46.
[32] 陈涛, 王贵美, 沈伟伟 , 等. 盐胁迫对红麻幼苗生长及抗氧化酶活性的影响. 植物科学学报, 2011,29(4):493-501.
doi: 10.3724/SP.J.1142.2011.40493
[33] 孙天国, 沙伟 . 盐胁迫对甜瓜种子萌发及幼苗SOD•POD活性的影响. 安徽农业科学, 2009,37(24):11509-11510.
doi: 10.3969/j.issn.0517-6611.2009.24.068
[34] 彭立新, 周黎君, 冯涛 , 等. 盐胁迫对沙枣幼苗抗氧化酶活性和膜脂过氧化的影响. 天津农学院学报, 2009,16(4):1-4.
[35] 宁建凤, 郑青松, 邹献中 , 等. 罗布麻对不同浓度盐胁迫的生理响应. 植物学报, 2010,45(6):689-697.
doi: 10.3969/j.issn.1674-3466.2010.06.006
[36] 程淑娟, 唐东芹, 刘群录 . 盐胁迫对两种忍冬属植物活性氧平衡的影响. 南京林业大学学报(自然科学版), 2013,37(1):137-141.
doi: 10.3969/j.issn.1000-2006.2013.01.025
[37] 艾力江•麦麦提, 齐曼•尤努斯, 公勤 . NaCl胁迫对三种胡颓子属植物幼苗活性氧清除酶与渗透调节物质含量的影响. 新疆农业科学, 2008,45(6):1069-1075.
[38] 张舟, 邬忠康, 陈志成 , 等. 盐胁迫对4个品种豇豆种子萌发的影响. 种子, 2004,33(3):19-24.
doi: 10.3969/j.issn.1001-4705.2014.03.006
[1] Wu Ruixiang, Yang Jianchun, Wang Liqin, Guo Xiujuan. Evaluation of the Adaptability of Flax Drought#br# Resistance Based on Multiple Statistics Analysis [J]. Crops, 2018, 34(5): 10-16.
[2] Xiaoyu Liang, Chunyu Lin, Shumei Ma, Yang Wang. Mining Elite Alleles for Germination Ability in Rice (Oryza sativa L.) under Salt and Alkaline Stress [J]. Crops, 2018, 34(4): 48-52.
[3] Xiaoyong Zhang,Youlian Yang,Shujiang Li,Rongchuan Xiong,Hong Xiang. Effects of Exogenous GA3 and 6-BA on Leaf Senescence in Low Temperature Stress of Virus-Free Potato Cutting Seedlings [J]. Crops, 2018, 34(4): 95-101.
[4] Kailun Zhang,Shouming Chen,Hong Yin,Bin Li,Liangwen Xie,Fan He. and Antioxidant Activity of Tobacco Seedlings under Salt Stress [J]. Crops, 2018, 34(3): 123-128.
[5] Jianxia Liu,Xiaodan Zhang,Runmei Wang,Feng Zhou,Wenying Liu,Zhiping Liu. Effects of Seed Soaking with 6-BA on Germination and Physiological Characteristics of Mung Bean under Salt Stress [J]. Crops, 2018, 34(1): 166-172.
[6] Nannan Lu,Lihua Yan,Chongke Zheng,Haibo Yin,Shanli Guo,Xianzhi Xie. Effects of Salt Stress on Growth and Agronomic Traits of Yanfeng 47 and Yanjing 456 [J]. Crops, 2017, 33(5): 106-111.
[7] Xiyu Hao,Hongdan Wang,Zhichao Yin,Jie Liang,Fengxiang Yin,Jianjun Hao. Effects of PEG Stress on Drought Resistance at Seedling Stage of Adzuki Beans and the Establishment of Drought Resistance Identification System [J]. Crops, 2017, 33(4): 134-142.
[8] ,Lili Zhang,Ying Shi. Screening of Drought Resistant Germplasm Resources in Potato [J]. Crops, 2017, 33(4): 72-77.
[9] Ning Yang,Botong Zhao,Yuzhuo Bao,Yan Lü,Kankan Peng,Yu Tian,Junhong Wang,Jing Meng,Jing Cang. Effects of Exogenous ABA and Its Inhibitor Fluridone on Cold Resistance Indicators of Winter Wheat Tiller Node [J]. Crops, 2017, 33(4): 117-122.
[10] Junying Wu,Li Qin,Jin Yang,Dezhi Qin. Study of Salt Stress on Sugar Beet Seedling Growth and Nutrient Transport of Nongda Tianyan No. 6 [J]. Crops, 2017, 33(3): 75-80.
[11] Ziwei Zhang,Chunhua Pang,Yongqing Zhang,Ruijun Ni,Shifang Yang,Luyuan Wang,Liqin Liu. Effects of Iso-osmotic NaCl and PEG Stress and Rewatering on Seed Germination and Seedling Growth of Quinoa [J]. Crops, 2017, 33(1): 119-126.
[12] Cuicui Yang,Fade Li,Yan Li,Chenglai Wu,Chunqing Zhang. Effects of Arc-Tooth-Shaped Corona Discharge Field Treatment on Improving Corn Seed Vigor and Physiological Indexes [J]. Crops, 2016, 32(6): 154-159.
[13] Zhurong Zheng,Ruixiang Zhang,Tingting Yang,Lichao Wen,Xuefeng Shen. Effects of Salt Stress on Physiological and Biochemical Characteristics of Roots in Peanut [J]. Crops, 2016, 32(4): 142-145.
[14] Limin Sang,Caifeng Li,Yubo Wang,Lei Liu,Guanghao Guo,Jian Guo,Ming Chen. Effects of Na2SO4+NaCl Mixed Salt Stress on the Growth of Sugar Beet Seedlings [J]. Crops, 2016, 32(4): 137-141.
[15] Yingmei Zuo,Weize Yang,Tianmei Yang,Meiquan Yang,Zongliang Xu,Shaobing Yang,Jinyu Zhang. Comparison of Resistant Physiological Index among Four Species in the Genus Panax under Water Stress [J]. Crops, 2016, 32(3): 84-88.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .