Crops ›› 2025, Vol. 41 ›› Issue (2): 228-233.doi: 10.16035/j.issn.1001-7283.2025.02.031

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Effects of Exogenous Betaine on Germination and Seedling Physiological Characteristics of Foxtail Millet under Drought Stress

Hou Xiaomin(), Yan Feng, Dong Yang, Zhao Fuyang, Li Qingquan(), Ji Shengdong, Liu Yue, Lan Ying   

  1. Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161006, Heilongjiang, China
  • Received:2024-05-23 Revised:2024-06-27 Online:2025-04-15 Published:2025-04-16

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Abstract:

The foxfail millet variety 'Nenxuan 19' was employed in this experiment, and 18% polyethylene glycol 6000 (PEG-6000) was chosen to simulate drought stress. The changes of seed germination index, seedling growth, antioxidant enzyme activity, membrane lipid peroxidation and osmotic adjustment substance content of foxtail millet under drought stress condition were analyzed by seed germination test and pot experiment. The results showed that the exogenous betaine could significantly increased the germination rate, germination potential and germination index of foxtail millet seeds under drought stress, as well as increased plant height, root length, shoot and root biomass of seedlings, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were significantly increased. At the same time, the relative water content, proline, soluble sugar and soluble protein contents were increased. Betaine could effectively reduce the root-shoot ratio, relative electrical conductivity and malondialdehyde (MDA) content of foxtail millet seedlings, and reduce the degree of membrane lipid peroxidation. Therefore, exogenous betaine can promote the germination and seedling growth of foxtail millet under drought stress, and promote osmotic adjustment ability and antioxidant ability, thus effectively alleviate the damage of drought stress to the growth and development of foxtail millet.

Key words: Foxtail millet, Betaine, Drought stress, Germination, Antioxidant enzyme

Table 1

Effect of betaine on millet seeds germination under drought stress"

处理
Treatment		 发芽率
Germination
rate (%)		 发芽势
Germination
potential (%)		 发芽指数
Germination
index
CK 85.0±2.8b 73.0±1.1b 120.6±1.9b
T 92.0±0.7a 88.0±1.3a 134.1±2.1a
S 48.0±2.3d 39.0±2.5d 103.8±1.5d
ST 62.0±3.8c 45.0±3.6c 115.0±2.7c

Table 2

Effects of betaine on biomass accumulation of foxtail millet seedlings under drought stress"

处理
Treatment		 鲜重Fresh weight (g) 干重Dry weight (g) 株高
Plant height (cm)		 根长
Root length (cm)		 根冠比
Root-shoot ratio
地上部Shoot 根系Root 地上部Shoot 根系Root
CK 0.521±0.028a 0.155±0.001ab 0.076±0.011a 0.016±0.002a 27.42±1.38ab 0.37±0.01b 0.25±0.04c
T 0.563±0.012a 0.173±0.018a 0.078±0.005a 0.015±0.001a 33.49±2.01a 0.48±0.02a 0.25±0.06c
S 0.232±0.035c 0.107±0.012c 0.053±0.002c 0.010±0.001b 19.67±1.03c 0.25±0.01c 0.35±0.02a
ST 0.367±0.056b 0.133±0.020bc 0.059±0.004b 0.017±0.003b 24.18±0.78b 0.34±0.01b 0.27±0.02b

Fig.1

Effects of betaine on the activities of SOD, POD, CAT and APX in foxtail millet seedlings under drought stress"

Fig.2

Effects of betaine on proline, soluble protein and soluble sugar contents of foxtail millet seedlings under drought stress"

Fig.3

Effects of betaine on MDA content of foxtail millet seedlings under drought stress"

Fig.4

Effects of betaine on relative electrical conductivity of foxtail millet seedlings under drought stress"

Fig.5

Effects of betaine on leaf relative water content of foxtail millet seedlings under drought stress"

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