Crops ›› 2017, Vol. 33 ›› Issue (1): 168-172.doi: 10.16035/j.issn.1001-7283.2017.01.030

Previous Articles    

Cytological Observation in Fusarium culmorum-Infected Potato Tuber

Sun Meili,Xu Yongqing,Shi Lijuan,Shan Weiyu,Chai Ying,Li Xiuyu,Li Lei,Li Fenglan   

  1. College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2016-09-05 Revised:2016-12-11 Online:2017-02-15 Published:2018-08-26

RichHTML

6

PDF (PC)

202

Abstract:

Potato dry rot causes corruption of stored potato tubers, affecting their commodity value. Fusarium culmorum is the main pathogenic fungus for potato dry rot in Heilongjiang province. In order to study the infection process of Fusarium in potato, we investigated a histological and cytological observation during Fusarium culmorum infection progress in potato tubers with different resistant(Kexin 1 and Daxiyang). The results showed that hyphae and spores of pathogens accumulated in potato tubers 2d after the infection and potato tubers rotted seriously 16d after the infection. The tubers lesion area in Kexin 1 was 44.44% of the total area, and that in Atlantic was 89.34%. This result indicate that the Fusarium culmorum can cause the large area damage of the inner structure of the potato tuber. By the microstructure observation, we found that there were a large number Fusarium culmorum mycelia in infected potato tubers 3d after the infection and the cell wall of potato tuber thickened and broke in the infection course.However this appeared relatively late for resistant variety, showing Kexin 1 had higher resistance to Fusarium culmorum than Daxiyang.

Key words: Potato, Dry rot, Fusarium culmorum, Cytological observation

Fig.1

Cytological observation of F.culmorum infection in potato tuber"

[1] 谢从华 . 马铃薯产业的现状与发展. 华中农业大学学报(社会科学版), 2012,97(1):1-4.
[2] 王云龙 . 黑龙江省马铃薯产业现状与展望. 中国马铃薯, 2014,28(3):185-188.
[3] 刁小琴, 关海宁, 魏雅冬 , 等. 窖藏马铃薯干腐病病原菌的分离及绿色防治技术研究. 食品研究与开发, 2015,36(1):122-125.
[4] 张俊祺, 代昌明, 杨军 , 等. 马铃薯Y病毒贵州黔西烟草分离物P1基因序列分析. 植物病理学报, 2012,4(1):88-92.
[5] 韩峰, 李凤兰, 李学湛 , 等. 马铃薯干腐病主要致病菌DNA提取方法比较. 中国农学通报, 2011,27(1):209-213.
[6] 林镇跃, 阙友雄, 刘平武 , 等. 植物致病镰刀菌的研究进展.中国糖料, 2014(1):58-64.
[7] Stevenson W R, Loria R, Franc G D , et al. Compendium of Potato Diseases.The American Phytopathological Society,St. Paul, MN, 2001.
[8] Mecteau M R, Arul J, Tweddell R J . Effect of organic and inorganic salts on the growth and development of Fusarium sambucinum,a causal agent of potato dry rot. Mycological Research, 2002,106(3):688-696.
doi: 10.1017/S0953756202005944
[9] Leslie J F, Sununerell B A. The Fusarium Laoratory Manual. Blackwell Publishing, 2006: 121-274.
[10] 石立航, 胡俊, 蒙美莲 , 等. 华北地区马铃薯贮藏病害种类调查及病原菌鉴定.内蒙古农业大学学报(自然科学版), 2010(4):53-57.
[11] Pearson K A, Taylor A F S, Fuchs R M E, et al. Characterisation and pathogenicity of Fusarium taxa isolated from ragwort (Jacobaea vulgaris) roots. Fungal Ecology, 2016,20:186-192.
doi: 10.1016/j.funeco.2015.12.011
[12] Valentina S, Amedeo R, Francesca V , et al. Relationship between European corn borer injury,Fusarium proliferatum and F.subglutinans infection and moniliformin contamination in maize. Field Crops Research, 2015,183:69-78.
doi: 10.1016/j.fcr.2015.07.014
[13] 刘晓燕, 罗振鹏, 崔伟康 , 等. 绿色荧光蛋白标记下镰刀菌侵染地黄的组织学观察. 菌物学报, 2015,34(6):1092-1100.
[14] 陈彦云 . 宁夏西吉县马铃薯贮藏期病害调查及药剂防治研究.耕作与栽培, 2007(3):15-16.
[15] 敏凡祥, 王晓丹, 胡林双 , 等. 黑龙江省马铃薯干腐病菌种类鉴定及致病性. 植物保护, 2010,36(4):112-115.
[16] 李凤兰, 蒋先锋, 史丽娟 , 等. 两株马铃薯干腐病病原菌的分离和鉴定.作物杂志, 2013(4):125-129.
[17] 左豫虎, 史洁, 韩青梅 , 等. 茄链格孢菌侵染马铃薯叶片过程的细胞学观察.中国蔬菜, 2011(14):35-40.
[18] 张文解, 王成明 . 马铃薯病虫害诊断与防治.甘肃: 甘肃科学技术出版社, 2010: 7-11.
[19] King B C, Waxman K D, Nenni N V , et al. Arsenal of plant cell wall degrading enzymes reflects host preference among plant pathogenic fungi. Biotechnology for Biofuels, 2011,4(1):363-368.
[20] 王金生 . 分子植物病理学.北京: 中国农业出版社, 2001: 21-22.
[21] 拓宁, 张君, 邱慧珍 , 等. 立枯丝核菌对马铃薯侵染过程的显微结构观察与胞壁降解酶活性的测定. 草业学报, 2015,24(12):74-82.
doi: 10.11686/cyxb2015051
[22] 康振生, 黄丽丽, 韩青梅 , 等. 禾谷镰刀菌侵染引致小麦穗组织细胞壁成分变化的细胞化学研究. 植物病理学报, 2007,37(6):623-628.
[23] 梁艳丽, 赵婧, 刘林 , 等. 植物细胞壁在植物与病原菌互作中的作用. 分子植物育种, 2016,14(5):1255-1261.
[24] 唐文菊, 赵庆芳, 李巧峡 , 等. 当归根显微结构及其根腐病真菌分布研究. 广西植物, 2015,35(4):487-492.
[25] 袁广胜, 赵茂俊, 张志明 , 等. 串珠镰刀菌引起玉米穗粒腐病防御酶变化及其电镜观察. 植物病理学报, 2011,41(4):385-392.
[1] Su Feifei, Zhang Jinghua, Li Yong, Liu Shangwu, Liu Zhenyu, Wang Shaopeng, Wan Shuming, Chen Xi, Gao Yunfei, Hu Linshuang, Lü Dianqiu. Effects of Different Irrigation Methods on#br# Physiological Characteristics and Water#br# Use Efficiency of Potato [J]. Crops, 2018, 34(5): 97-103.
[2] Ying Chai,Yongqing Xu,Yao Fu,Xiuyu Li,Fumeng He,Yingqi Han,Zhe Feng,Fenglan Li. Characteristics of Cell Wall Degradation Enzyme Produced by Main Pathogenic Fusarium spp. in Potato Dry Rot [J]. Crops, 2018, 34(4): 154-160.
[3] Yajun Liu,Qiguo Hu,Fengli Chu,Wenjing Wang,Aimei Yang. Effects of Different Cultivation Methods and Planting Densities on the Yield and Storage Root Tuberization of Sweet Potato cv. "Shangshu 9" [J]. Crops, 2018, 34(4): 89-94.
[4] Xiaoyong Zhang,Youlian Yang,Shujiang Li,Rongchuan Xiong,Hong Xiang. Effects of Exogenous GA3 and 6-BA on Leaf Senescence in Low Temperature Stress of Virus-Free Potato Cutting Seedlings [J]. Crops, 2018, 34(4): 95-101.
[5] Yiran Ye, ,Bencai Sha,Wenxiang Wang,Hongda Ye,Shixian Geng,Jingjin Cheng,Meirong Hai. Effects of Different Fertilizers on Photosynthetic Characteristics of Winter Potato [J]. Crops, 2018, 34(3): 135-140.
[6] Yong Cui. The Research Progress of Water-Dry Rotation Methods in Paddy Field [J]. Crops, 2018, 34(3): 8-14.
[7] Shumin Liang,Ying Wang,Zhechao Pan,Lei Zhang,Ningsheng Xu,Yanshan Li,Qiongfen Yang,Xianping Li,Jianming ai,Chunguang Yao,Lili Lu,Qijun Sui. Effects of Soil Moisture and Temperature with Different Cultivation Methods on the Yield and Tuberization of Potato [J]. Crops, 2018, 34(3): 90-96.
[8] Xiaohua Shi,Haiying Yang,Wenqin Kang,Yonglin Qin,Mingshou Fan,Liguo Jia. Effects of Nitrogen Fertilization on Crop Yields and Soil Nitrogen Balance in Potato-Wheat System [J]. Crops, 2018, 34(2): 108-116.
[9] Yong Cui. Influence of Continuous Cropping and Controlling Measures on Continuous Cropping Potato [J]. Crops, 2018, 34(2): 87-92.
[10] Mingming Yan,Qiujun Chen,Min Tang,Zhiwen Liu. Effects of Different Concentration Combinations of Hormone and Organics on Rapid Propagation of Sweet Potato Virus-Free Seedling [J]. Crops, 2018, 34(2): 68-72.
[11] Shumin Liang,Lei Zhang,Shengding He,Qunqing Yang,Yanshan Li,Pinggen He,Shaolin Wang,Ying Wang,Qiongfen Yang,Qijun Sui. Yield and Economic Benefit of Potato under the Different Cultivation Patterns in Northwest Yunnan [J]. Crops, 2017, 33(6): 79-83.
[12] Qian Liu,Song Hou,Qing Liu,Huan Li,Yanxi Shi. Effects of Transplanting Date on Yield and Quality of Edible Sweet Potato cv. Yanshu No.25 [J]. Crops, 2017, 33(5): 136-141.
[13] Hongda Ye, ,Bencai Sha,Wenxiang Wang,Jia Liu,Yiran Ye,Meirong Hai. Effects of Altitude on Photosynthetic and Fluorescence Characteristics, and Antioxidant Properties on Solanum tuberosum L. [J]. Crops, 2017, 33(5): 93-99.
[14] Yue Huang,Shuaibing Li,Ying Shi. Differences in Mineral Nutrition Qualityof Different Potato Varieties [J]. Crops, 2017, 33(4): 33-37.
[15] ,Lili Zhang,Ying Shi. Screening of Drought Resistant Germplasm Resources in Potato [J]. Crops, 2017, 33(4): 72-77.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .