Crops ›› 2020, Vol. 36 ›› Issue (2): 176-181.doi: 10.16035/j.issn.1001-7283.2020.02.027

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Isolation and Identification of IAA - Producing Rhizobacteria and Its Effects on Seed Germination and Seedling Growth of Tobacco

Chen Yue,Li Hulin,Zhu Shimiao,Yan Han,Lang Bin,Ji Wenxiu   

  1. College of Agriculture, Yanbian University, Yanji 133002, Jilin, China
  • Received:2019-09-25 Revised:2019-12-28 Online:2020-04-15 Published:2020-04-13

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Abstract:

In order to obtain the high-quality bacteria resources and to explore their effects on the seed germination and seedling growth of tobacco, we isolated and screened IAA-producing strains from rhizosphere soil of tobacco in Yanbian area and carried out 16S rRNA sequencing identification, as well as investigated the effects of the single and mixed bacteria on the germination and seedling growth of tobacco. The results showed that both Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 produced more IAA than other strains, and the optimal culture time of m-53, m-60 and their mixture for growth were 24, 48 and 48h, and their corresponding IAA yields were 98, 41 and 141mg/L, respectively. Compared with the control, both single and mixed bacteria could promote seed germination and seedling growth of tobacco, especially the mixed bacteria had the best promotion effect. Sphingobacterium sp. m-53 and Jeotgalicoccus sp. m-60 can be applied separately or in combination as an alternative resource for microbial fertilizers of tobacco.

Key words: Tobacco, Plant growth-promoting rhizobacteria, Indole acetic acid, Promoting effect

Table 1

The ability of IAA production of rhizosphere bacteria from tobacco mg/L"

编号No. IAA含量IAA content 编号No. IAA含量IAA content 编号No. IAA含量IAA content
m-53 43.06±3.17a m-125 19.14±0.79cdefg m-113 15.47±0.88fghi
m-60 41.37±5.56a m-10 18.42±1.47cdefgh m-109 15.47±0.30fghi
m-75 27.95±2.79b m-7 18.28±1.54cdefgh m-8 15.37±1.48fghi
m-118 25.81±2.64bc m-74 17.89±0.28cdefgh m-97 14.70±1.07fghi
m-48 24.45±1.77bcd m-119 16.36±1.55defghi m-64 10.86±2.54ghi
m-72 24.36±1.68bcd m-117 16.09±2.35efghi m-129 10.34±0.76hi
m-89 22.28±2.59bcdef m-90 16.06±0.30efghi m-130 10.34±0.33i
m-65 20.23±2.09bcdef m-102 15.75±1.01efghi m-4 9.31±2.04i
m-66 19.73±0.89cdef m-122 15.59±1.99fghi m-24 8.31±0.17i

Table 2

Comparison of 16S rRNA sequence and GenBank-BLAST of bacteria"

编号
No.		 相似菌属
Similar genera		 相似度
Similarity (%)		 登录号
Login ID
m-53 鞘氨醇杆菌属Sphingobacterium 100 KC009697.1
m-60 咸海鲜球菌属Jeotgalicoccus 100 KT989860.1

Fig.1

Morphology of m-53 and m-60 strains"

Fig.2

The results of antagonistic reaction between m-53 and m-60 strains"

Fig.3

The effects of culture time on the growth of strain and its combination"

Fig.4

The effects of culture time on IAA production ability of strain and its combination"

Table 3

The effects of bacteria on germination indexes of tobacco seeds"

处理
Treatment		 发芽势
Germination
potential (%)		 发芽率
Germination
rate (%)		 发芽指数
Germination
index		 活力指数
Vitality
index
CK 56.67±8.74c 86.00±6.66a 0.90±0.02b 2.41±0.06c
m-53 66.00±2.65b 89.67±1.53a 0.95±0.02b 2.64±0.06b
m-60 66.00±8.19b 87.00±4.00a 0.95±0.06b 2.56±0.15bc
m-53 + m-60 72.67±2.08a 88.67±4.51a 1.12±0.02a 3.23±0.06a

Table 4

The effects of bacteria on agronomic traits of tobacco seedlings"

处理
Treatment		 株高(cm)
Plant height		 根长(cm)
Root length		 最大叶长(cm)
Maximum leaf length		 最大叶宽(cm)
Maximum leaf width		 茎围(mm)
Stem circumference		 叶片数
Number of leaves
CK 2.34±0.11c 2.06±0.38c 1.36±0.05c 1.24±0.05c 0.050±0.007c 3.02±0.04c
m-53 3.26±0.11b 2.54±0.09b 2.08±0.08b 1.66±0.05b 0.084±0.005b 3.16±0.11ab
m-60 2.28±0.13c 1.76±0.38d 1.36±0.05c 1.16±0.05c 0.050±0.006c 3.04±0.05c
m-53 + m-60 3.66±0.09a 4.42±0.43a 2.32±0.28a 1.82±0.04a 0.118±0.026a 3.20±0.07a

Fig.5

The effects of growth-promoting bacteria on the growth of tobacco seedlings"

Table 5

The effects of bacteria on biomass of tobacco seedlings"

处理
Treatment		 地上部Shoot 地下部Root 根冠比Root-shoot ratio
鲜重Fresh weight (mg) 干重Dry weight(mg) 鲜重Fresh weight (mg) 干重Dry weight (mg) 鲜重Fresh weight 干重Dry weight
CK 46.00±1.00c 2.67±0.41c 3.30±0.07c 0.28±0.10c 0.072±0.022b 0.109±0.045b
m-53 91.20±1.64b 5.99±0.17b 11.10±0.20b 0.92±0.05b 0.122±0.002a 0.154±0.005ab
m-60 41.40±0.55d 1.59±0.01d 3.00±0.22c 0.29±0.13c 0.072±0.029b 0.181±0.071a
m-53 + m-60 106.20±4.21a 7.02±0.36a 14.24±1.23a 1.28±0.19a 0.134±0.011a 0.184±0.032a
[1] Spaepen S, Versees W, Gocke D , et al. Characterization of phenylpyruvate decarboxylase,involved in auxin production of Azospirillum brasilense. Journal of Bacteriology, 2007,189(21):7626-7633.
[2] 张东艳, 刘晔, 吴越 , 等. 花生根际产lAA菌的筛选鉴定及其效应研究. 中国油料作物学报, 2016,38(1):104-110.
[3] Pothier J F, Wisniewski-Dyé F, Weiss-Gayet M , et al. Promoter-trap identification of wheat seed extract induced-genes in the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245. Microbiology, 2007,153(10):3608-3622.
[4] Baig D N, Mehnaz S . Determination and distribution of cry-type genes in halophilc Bacillus thuringiensis isolates of Arabian Sea sedimentary rocks. Microbiological Research, 2009,165(5):376-383.
[5] 李婉, 刘淼, 张必弦 , 等. 植物根际促生菌的研究进展及其应用现状. 中国农学通报, 2014,30(24):1-5.
[6] 徐立国, 方换男, 肖云峰 , 等. 烟草根际溶磷解钾细菌的筛选鉴定及对烟草的促生作用. 山东农业科学, 2018,50(3):107-112.
[7] 邢芳芳, 高明夫, 胡兆平 , 等. 1株高产IAA菌株的筛选、鉴定及对白菜的促生作用. 江苏农业科学, 2016,44(10):458-460.
[8] 汪钱龙, 张德智, 王菊芬 , 等. 不同植物促生细菌对玉米生长的影响及其生长素分泌能力研究. 云南农业大学学报, 2015,30(4):494-498.
[9] Sun S L, Yang W L, Fang W W , et al. The plant growth-promoting rhizobacterium Variovorax boronicumulans CGMCC 4969 regulates the level of indole-3-acetic acid synthesized from indole-3-acetonitrile. Applied and Environmental Microbiology, 2018: e00298-18.
[10] 王呈玉, 温芳悦, 萧龙珍 , 等. 溶磷菌剂发酵工艺条件的优化及其促生效果. 吉林农业大学学报, 2019,41(4):408-413.
[11] 王勇, 朱洪磊, 郭洁心 , 等. 梓树内生解淀粉芽孢杆菌GZ-5发酵条件的优化及其促生作用测定. 山西农业科学, 2019,47(2):152-155.
[12] 闫寒, 李虎林, 郎彬 , 等. 吉烟9号内生细菌的分离及多样性分析. 中国烟草科学, 2019,40(1):75-81.
[13] 崔月贞, 万志文, 冯疆荣 , 等. 东祁连山高寒草地优势牧草内生细菌产IAA能力的研究. 草地学报, 2016,24(3):618-623.
[14] 李振东, 陈秀蓉, 李鹏 , 等. 珠芽蓼内生菌Z5产IAA和抑菌能力测定及其鉴定. 草业学报, 2010,19(2):61-68.
[15] 科瓦尔库克, 德·布鲁金, 海德 , 等. 分子微生物生态学手册. 丁雄译. 2版. 荷兰多德雷赫特:克卢沃学术出版社, 2004.
[16] 李引, 虞丽, 李辉信 , 等. 一株花生根际促生菌的筛选鉴定及其特性研究. 生态与农村环境学报, 2012,28(4):416-421.
[17] 陈波, 丁延芹, 马海林 , 等. 樱桃根际促生细菌的筛选与鉴定. 微生物学通报, 2012,39(12):1746-1754.
[18] 吴翔, 谢丽源, 谭昊 , 等. 响应面法优化细菌MY07产lAA液体发酵培养基. 生物技术, 2015,25(2):201-204.
[19] 张英, 孙广正, 马骢毓 , 等. 披碱草根际促生菌筛选及其接种剂的促生作用. 植物营养与肥料学报, 2016,22(4):1039-1048.
[20] Chen L H, Yang X M, Raza W , et al. Solid-state fermentation of agro-industrial wastes to produce bioorganic fertilizer for the biocontrol of Fusarium wilt of cucumber in continuously cropped soil. Bioresource Technology, 2011,102(4):3900-3910.
[21] 徐婧, 邵锴, 李东芳 , 等. 产IAA菌的筛选、鉴定及其培养基的优化. 苏州科技大学学报(自然科学版), 2018,35(2):45-54.
[22] Casimiro I, Beeckman T, Graham N , et al. Dissecting Arabidopsis lateral root development. Trends in Plant Science, 2003,8(4):165-171.
[23] 王婧 . 桔黄假单胞菌JD37菌株对植物的促生作用及其微生物肥料的研制. 上海:上海师范大学, 2012.
[24] Zemrany H E, Czarnes S, Hallett P D , et al. Early changes in root characteristics of maize (Zea mays) following seed inoculation with the PGPR Azospirllnm lipoferum CRT1. Plant and Soil, 2007,291(1/2):109-118.
[25] 王辰月, 陈秀蓉, 杨成德 . 分泌吲哚乙酸洽草内生细菌的筛选及其对种子发芽的影响. 草原与草坪, 2013,33(1):21-24.
[26] 刘青海 . 六株溶磷菌与四株固氮菌互作效应及其菌剂对苜蓿促生效果研究. 兰州:甘肃农业大学, 2011.
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